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The map label for this gene is dacF [H]

Identifier: 83593448

GI number: 83593448

Start: 2451696

End: 2453102

Strand: Direct

Name: dacF [H]

Synonym: Rru_A2113

Alternate gene names: 83593448

Gene position: 2451696-2453102 (Clockwise)

Preceding gene: 83593442

Following gene: 83593455

Centisome position: 56.32

GC content: 67.52

Gene sequence:

>1407_bases
ATGAACCATTTGCCGGAACTTCCGTCATCCGGCCGTGCCGGCTCTGGCGCGACGGAGCCGGCGGCGGGGCGCCTTCGGAT
CCTGGCGATGCTTGCCGTTGTTTTCCTGCTGCTCGGCCTGCCCACCCAGGCCTTCGCCGGCTACGCCTCGATCGTCGTTG
ACGCGTCGACGGGCCGGGTTTTGCAGGCGCGCAACGCCGATATGCGCAACTACCCCGCCTCGCTGACCAAGATGATGACC
CTTTATCTGCTGTTCGAGGCGATCGACAACGGCAAATACACCCTGGAAAGCCGGCTGCCCGTGTCGAAATGGGCGGCCAA
TCAGGCGCCGTCCAAGCTGGGGGTGGCCGCGGGGACCGGCATCAAGGTTGAACACGCGATCAAGGCCCTGGTCACCAAAT
CGGCCAATGACGTGGCCGTGGTTATCGCCGAGGCCCTGGGCGGCACCGAAGCCAATTTCTCGGCCCTGATGACCCGCAAG
GCGGCGGCGCTCGGCATGAGCCAGACCACCTTTCGCAACGCCTCGGGCCTGCCCAACAACGGCCAGATGACCACCGCCCG
TGACATGGCGGTGCTGTCGATGGCGCTGATCCGCCATTTTCCCAACCATTACCGCTTCTTCGCCACCCAGTCCTTCCGCT
TTGGCAACCGGACGATCACCACCCATAACCATGTCGTGCGCGAATACCCCGGGGCCGATGGCCTGAAGACCGGCTATATC
CAGGCCTCGGGCTTCAATGTCGCCTCCTCGGCGGTGCGCAATGGCCGTCGTCTGGTCGCCGTGGTCTTCGGCGGACAGAC
GGCGCGGGCCCGCGACACCCAGATGATGGCCCTGCTGGACGAGGGCTTCGCCACCCTGGCCCGCGAAGGCGGCAGCGTCG
TTCAGGCCAAGCGCAAACCCGATGCCATCGCCTCGATCATCAAAGCCAAGGAACCGCCGGCCGCCGATATGGGCTCGGCC
GACGACGCGCCGATGCCCCCGGCCTACAAGCCCGGCTCGCTGACCGCCTCGGTCAGCGGCGGACGGACCCAGCAGATGGC
CCTGGCCACCCCAATGCGCTCCTCGCCCGCTCCCTCGGCCAAGAGCGCCCCCGGCCCCCGGCCAACCCCCATCGCCCGCA
CGACGCCCGTCCCAAGCAAGGGCGCGGCCAAGGGGGACTGGGGCATTCAGGTTGGCGCCTATGCCAAATCGGCGGCGGCG
ACGGCGGCGGCCAATAAGGCCATCGGCCTTCTCGCCGCCGAGGCCCGCAGGCAGGTTTCGCCCGTCGTCGTTCAGCGCAA
AAGCGGATCTGGGACCATTTATCGGGCACGGCTGACCGGATTGTCCAACGAGGCGGCGGCCCGCACCGCCTGCGCCACCC
TCGTCCGCAACGACCATTCCTGTCTGGTCGTCGCCCCGCAGAAATAG

Upstream 100 bases:

>100_bases
ATTCGGGCTTCAAGCGTGGACACCGACTCGTCCTTGCCATATTCTATCCATCCATTCTTACCCGAACGACGCCGCGTTCA
ACACTGATCATGGGGTAGCG

Downstream 100 bases:

>100_bases
CGCCCCCGAACTCTGGCGCCGGCCCCTAACACCGGCCCTGGCGCCCGGTCGCCAGGGCGCCGGCCATCAGGCAGCCGGCG
ACGGCGGCGGCGCAGAGCAC

Product: serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MNHLPELPSSGRAGSGATEPAAGRLRILAMLAVVFLLLGLPTQAFAGYASIVVDASTGRVLQARNADMRNYPASLTKMMT
LYLLFEAIDNGKYTLESRLPVSKWAANQAPSKLGVAAGTGIKVEHAIKALVTKSANDVAVVIAEALGGTEANFSALMTRK
AAALGMSQTTFRNASGLPNNGQMTTARDMAVLSMALIRHFPNHYRFFATQSFRFGNRTITTHNHVVREYPGADGLKTGYI
QASGFNVASSAVRNGRRLVAVVFGGQTARARDTQMMALLDEGFATLAREGGSVVQAKRKPDAIASIIKAKEPPAADMGSA
DDAPMPPAYKPGSLTASVSGGRTQQMALATPMRSSPAPSAKSAPGPRPTPIARTTPVPSKGAAKGDWGIQVGAYAKSAAA
TAAANKAIGLLAAEARRQVSPVVVQRKSGSGTIYRARLTGLSNEAAARTACATLVRNDHSCLVVAPQK

Sequences:

>Translated_468_residues
MNHLPELPSSGRAGSGATEPAAGRLRILAMLAVVFLLLGLPTQAFAGYASIVVDASTGRVLQARNADMRNYPASLTKMMT
LYLLFEAIDNGKYTLESRLPVSKWAANQAPSKLGVAAGTGIKVEHAIKALVTKSANDVAVVIAEALGGTEANFSALMTRK
AAALGMSQTTFRNASGLPNNGQMTTARDMAVLSMALIRHFPNHYRFFATQSFRFGNRTITTHNHVVREYPGADGLKTGYI
QASGFNVASSAVRNGRRLVAVVFGGQTARARDTQMMALLDEGFATLAREGGSVVQAKRKPDAIASIIKAKEPPAADMGSA
DDAPMPPAYKPGSLTASVSGGRTQQMALATPMRSSPAPSAKSAPGPRPTPIARTTPVPSKGAAKGDWGIQVGAYAKSAAA
TAAANKAIGLLAAEARRQVSPVVVQRKSGSGTIYRARLTGLSNEAAARTACATLVRNDHSCLVVAPQK
>Mature_468_residues
MNHLPELPSSGRAGSGATEPAAGRLRILAMLAVVFLLLGLPTQAFAGYASIVVDASTGRVLQARNADMRNYPASLTKMMT
LYLLFEAIDNGKYTLESRLPVSKWAANQAPSKLGVAAGTGIKVEHAIKALVTKSANDVAVVIAEALGGTEANFSALMTRK
AAALGMSQTTFRNASGLPNNGQMTTARDMAVLSMALIRHFPNHYRFFATQSFRFGNRTITTHNHVVREYPGADGLKTGYI
QASGFNVASSAVRNGRRLVAVVFGGQTARARDTQMMALLDEGFATLAREGGSVVQAKRKPDAIASIIKAKEPPAADMGSA
DDAPMPPAYKPGSLTASVSGGRTQQMALATPMRSSPAPSAKSAPGPRPTPIARTTPVPSKGAAKGDWGIQVGAYAKSAAA
TAAANKAIGLLAAEARRQVSPVVVQRKSGSGTIYRARLTGLSNEAAARTACATLVRNDHSCLVVAPQK

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1786851, Length=240, Percent_Identity=34.1666666666667, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1787062, Length=273, Percent_Identity=34.7985347985348, Blast_Score=135, Evalue=6e-33,
Organism=Escherichia coli, GI87082022, Length=249, Percent_Identity=32.9317269076305, Blast_Score=131, Evalue=9e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 48825; Mature: 48825

Theoretical pI: Translated: 11.08; Mature: 11.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHLPELPSSGRAGSGATEPAAGRLRILAMLAVVFLLLGLPTQAFAGYASIVVDASTGRV
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCEEEEEEECCCCCE
LQARNADMRNYPASLTKMMTLYLLFEAIDNGKYTLESRLPVSKWAANQAPSKLGVAAGTG
EEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHCCCCCCCCCEECCCC
IKVEHAIKALVTKSANDVAVVIAEALGGTEANFSALMTRKAAALGMSQTTFRNASGLPNN
CCHHHHHHHHHHCCCCCEEEEEEHHHCCCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCC
GQMTTARDMAVLSMALIRHFPNHYRFFATQSFRFGNRTITTHNHVVREYPGADGLKTGYI
CCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCEEEECCCCHHHHCCCCCCCCCCEE
QASGFNVASSAVRNGRRLVAVVFGGQTARARDTQMMALLDEGFATLAREGGSVVQAKRKP
EECCCCHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCH
DAIASIIKAKEPPAADMGSADDAPMPPAYKPGSLTASVSGGRTQQMALATPMRSSPAPSA
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEECCCCCCCCCCC
KSAPGPRPTPIARTTPVPSKGAAKGDWGIQVGAYAKSAAATAAANKAIGLLAAEARRQVS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCC
PVVVQRKSGSGTIYRARLTGLSNEAAARTACATLVRNDHSCLVVAPQK
CEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCC
>Mature Secondary Structure
MNHLPELPSSGRAGSGATEPAAGRLRILAMLAVVFLLLGLPTQAFAGYASIVVDASTGRV
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCEEEEEEECCCCCE
LQARNADMRNYPASLTKMMTLYLLFEAIDNGKYTLESRLPVSKWAANQAPSKLGVAAGTG
EEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHCCCCCCCCCEECCCC
IKVEHAIKALVTKSANDVAVVIAEALGGTEANFSALMTRKAAALGMSQTTFRNASGLPNN
CCHHHHHHHHHHCCCCCEEEEEEHHHCCCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCC
GQMTTARDMAVLSMALIRHFPNHYRFFATQSFRFGNRTITTHNHVVREYPGADGLKTGYI
CCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCEEEECCCCHHHHCCCCCCCCCCEE
QASGFNVASSAVRNGRRLVAVVFGGQTARARDTQMMALLDEGFATLAREGGSVVQAKRKP
EECCCCHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCH
DAIASIIKAKEPPAADMGSADDAPMPPAYKPGSLTASVSGGRTQQMALATPMRSSPAPSA
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEECCCCCCCCCCC
KSAPGPRPTPIARTTPVPSKGAAKGDWGIQVGAYAKSAAATAAANKAIGLLAAEARRQVS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCC
PVVVQRKSGSGTIYRARLTGLSNEAAARTACATLVRNDHSCLVVAPQK
CEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]