Click here to switch to the map view.

The map label for this gene is luxO [H]

Identifier: 83593436

GI number: 83593436

Start: 2435742

End: 2437178

Strand: Reverse

Name: luxO [H]

Synonym: Rru_A2101

Alternate gene names: 83593436

Gene position: 2437178-2435742 (Counterclockwise)

Preceding gene: 83593437

Following gene: 83593435

Centisome position: 55.99

GC content: 65.48

Gene sequence:

>1437_bases
ATGCACCGTATCTTGATGGTGGAGGATTCGCCCTCCAACGCCGAACTTTACGCCACCTATCTGAGGGCGTCGGGGATGAC
CGTCACCTTGGCCGGGACGGGAGGGCAGGCCCTCGACTATCTGGTCGCCGATGCCAGCGCCTTCGATCTGGTGGTGCTCG
ATCTCGGTTTGCCTGATATGGACGGCCTGGAAATCCTGCGCAAGCTGGGCGGTCCGGCGGCGATGCCGCCGACTGTCGTG
CTGACCGGGTCGGGATCGGTGCGCCGCGCCGTCGAGGCGATGCGCGAGGGGGCGATCGATTTCCTGGTCAAGCCCTGCGG
CGCCGACAAACTGCGCACCGCCGTTGAAGAGGCTTTGGAGCGGCGCACGCCCACCGCCGAAGGCCGGGCGCCGGCCGCCG
ATCCGACCCGGGCCAAACGCGGCCCCTCGGGTTTTCTGGGCTCCTCACCGGCCATGTCCAACGTCTTTAGCTTGATCGAG
GCGGCGGCGGCCAGCAGCACCGCCAGCGTGTTCATCACCGGCGAGACCGGGACGGGCAAGGAACTCTGCGCCAACGCCAT
CCATAATCTGTCGGACCGTCGCAACGGCCCCTTCGTCGCCATCAACTGCGGCGCCATTCCCCGCGAATTGATGGAAAGCG
AGATCTTCGGCCATCGCAAGGGCGCCTTCACCGGGGCGATCAACGACCGCGAGGGCGCGGCCAGCCGGGCCGATGGCGGA
ACCCTGTTCCTTGATGAACTGTGCGAGATGGACCTCGATCTTCAGGTGAAGCTGCTGCGCTTCATCCAGACCGGCACCTA
TCGCAAGGTGGGCGACGGCGCCGATCGTCAGGTCAATATCCGCTTCGTCTGCGCCACCAATCGCGATCCGCTGGTCGAGG
TCAAGGCCGGGCGCTTCCGCGAGGATCTGTATTACCGGCTTTATGTGGTGCCCATCCACCTGCCGCCGCTTTCGGCGCGC
GGCGATGACATCATCTTGCTGGCCCGGCGGTTTCTCGCCGATTTCTCCAAGCTCGAAGGCAAGCGCTTCCAGGAGTTCTC
GGCCGATGCCCTGGGGCGGATCATGGCCTATCGCTGGCCGGGCAATGTCCGCCAGCTTGAAAACGTCGTGCGCAATATCG
TCGTGCTCAATGACGGGGTGACGGTCGAGCCGACCATGTTGCCACCGCCGCTTGACTGCGTCGAAGCCGTGGTCAGCCTG
CCCGTCGACCCGCAAAGCGCCACCGGGGCCGTTGTTGGCGGGGGAACGCTGTCGCTTGATGAGATCCTCGCCCGCCCCTT
GGCCGAACTGGAAGCCATGGCCATCGACGCCGCCTTGCGGTTGTCGGACAACAATGTGACGCGGGCGGCGCGGCTTCTTG
ATGTCTCGCCCTCGACCATCTATCGCAAGCATTCCGCCTATAAATCGGGCGGAACGGGCGGAAGCGCCGGGCGCTGA

Upstream 100 bases:

>100_bases
GGCGCGAGGCGACGATAGACGTGGCTTCGGGCGACGGCTATGGTGGACGCAACCCATGATTAGGGGGTCACGGTAAGGGA
TCGAAGGGTCTTTGGCGTCC

Downstream 100 bases:

>100_bases
GGCTTGGGGCCGGTCCGGGGCTTGCAACCGGGGCGGCGTCGGGGTCTTATCGAGATCAGCCCCCCGCAAGGTCGCCACGC
GCGAACCCGTCCCGGAAAGC

Product: two component, sigma54 specific, Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MHRILMVEDSPSNAELYATYLRASGMTVTLAGTGGQALDYLVADASAFDLVVLDLGLPDMDGLEILRKLGGPAAMPPTVV
LTGSGSVRRAVEAMREGAIDFLVKPCGADKLRTAVEEALERRTPTAEGRAPAADPTRAKRGPSGFLGSSPAMSNVFSLIE
AAAASSTASVFITGETGTGKELCANAIHNLSDRRNGPFVAINCGAIPRELMESEIFGHRKGAFTGAINDREGAASRADGG
TLFLDELCEMDLDLQVKLLRFIQTGTYRKVGDGADRQVNIRFVCATNRDPLVEVKAGRFREDLYYRLYVVPIHLPPLSAR
GDDIILLARRFLADFSKLEGKRFQEFSADALGRIMAYRWPGNVRQLENVVRNIVVLNDGVTVEPTMLPPPLDCVEAVVSL
PVDPQSATGAVVGGGTLSLDEILARPLAELEAMAIDAALRLSDNNVTRAARLLDVSPSTIYRKHSAYKSGGTGGSAGR

Sequences:

>Translated_478_residues
MHRILMVEDSPSNAELYATYLRASGMTVTLAGTGGQALDYLVADASAFDLVVLDLGLPDMDGLEILRKLGGPAAMPPTVV
LTGSGSVRRAVEAMREGAIDFLVKPCGADKLRTAVEEALERRTPTAEGRAPAADPTRAKRGPSGFLGSSPAMSNVFSLIE
AAAASSTASVFITGETGTGKELCANAIHNLSDRRNGPFVAINCGAIPRELMESEIFGHRKGAFTGAINDREGAASRADGG
TLFLDELCEMDLDLQVKLLRFIQTGTYRKVGDGADRQVNIRFVCATNRDPLVEVKAGRFREDLYYRLYVVPIHLPPLSAR
GDDIILLARRFLADFSKLEGKRFQEFSADALGRIMAYRWPGNVRQLENVVRNIVVLNDGVTVEPTMLPPPLDCVEAVVSL
PVDPQSATGAVVGGGTLSLDEILARPLAELEAMAIDAALRLSDNNVTRAARLLDVSPSTIYRKHSAYKSGGTGGSAGR
>Mature_478_residues
MHRILMVEDSPSNAELYATYLRASGMTVTLAGTGGQALDYLVADASAFDLVVLDLGLPDMDGLEILRKLGGPAAMPPTVV
LTGSGSVRRAVEAMREGAIDFLVKPCGADKLRTAVEEALERRTPTAEGRAPAADPTRAKRGPSGFLGSSPAMSNVFSLIE
AAAASSTASVFITGETGTGKELCANAIHNLSDRRNGPFVAINCGAIPRELMESEIFGHRKGAFTGAINDREGAASRADGG
TLFLDELCEMDLDLQVKLLRFIQTGTYRKVGDGADRQVNIRFVCATNRDPLVEVKAGRFREDLYYRLYVVPIHLPPLSAR
GDDIILLARRFLADFSKLEGKRFQEFSADALGRIMAYRWPGNVRQLENVVRNIVVLNDGVTVEPTMLPPPLDCVEAVVSL
PVDPQSATGAVVGGGTLSLDEILARPLAELEAMAIDAALRLSDNNVTRAARLLDVSPSTIYRKHSAYKSGGTGGSAGR

Specific function: Acts negatively to control the expression of luminescence. At low cell density, luxO is phosphorylated, and together with sigma-54, causes repression of the luxCDABEGH operon. This repression could be indirect, luxO could activate a negative regulator of

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=462, Percent_Identity=40.4761904761905, Blast_Score=307, Evalue=9e-85,
Organism=Escherichia coli, GI1788550, Length=470, Percent_Identity=38.7234042553192, Blast_Score=294, Evalue=7e-81,
Organism=Escherichia coli, GI1788905, Length=468, Percent_Identity=36.5384615384615, Blast_Score=283, Evalue=2e-77,
Organism=Escherichia coli, GI1790299, Length=469, Percent_Identity=36.8869936034115, Blast_Score=278, Evalue=7e-76,
Organism=Escherichia coli, GI87082152, Length=326, Percent_Identity=42.3312883435583, Blast_Score=221, Evalue=1e-58,
Organism=Escherichia coli, GI1789233, Length=326, Percent_Identity=41.4110429447853, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI87082117, Length=239, Percent_Identity=47.2803347280335, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI1789087, Length=250, Percent_Identity=45.6, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI87081872, Length=309, Percent_Identity=39.4822006472492, Blast_Score=192, Evalue=5e-50,
Organism=Escherichia coli, GI1787583, Length=284, Percent_Identity=38.7323943661972, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1786524, Length=258, Percent_Identity=42.2480620155039, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI87081858, Length=305, Percent_Identity=30.1639344262295, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1789828, Length=187, Percent_Identity=39.572192513369, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1786784, Length=119, Percent_Identity=33.6134453781513, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI1786911, Length=125, Percent_Identity=36.8, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 51073; Mature: 51073

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRILMVEDSPSNAELYATYLRASGMTVTLAGTGGQALDYLVADASAFDLVVLDLGLPDM
CCEEEEEECCCCCCCEEEEEEECCCCEEEEECCCCHHHHHHHCCCCCEEEEEEECCCCCC
DGLEILRKLGGPAAMPPTVVLTGSGSVRRAVEAMREGAIDFLVKPCGADKLRTAVEEALE
HHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHH
RRTPTAEGRAPAADPTRAKRGPSGFLGSSPAMSNVFSLIEAAAASSTASVFITGETGTGK
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCH
ELCANAIHNLSDRRNGPFVAINCGAIPRELMESEIFGHRKGAFTGAINDREGAASRADGG
HHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCC
TLFLDELCEMDLDLQVKLLRFIQTGTYRKVGDGADRQVNIRFVCATNRDPLVEVKAGRFR
EEEHHHHHCCCCCHHHHHHHHHHCCCCEECCCCCCCEEEEEEEEECCCCCEEEECCCCHH
EDLYYRLYVVPIHLPPLSARGDDIILLARRFLADFSKLEGKRFQEFSADALGRIMAYRWP
CCEEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHEEEECC
GNVRQLENVVRNIVVLNDGVTVEPTMLPPPLDCVEAVVSLPVDPQSATGAVVGGGTLSLD
CCHHHHHHHHHEEEEECCCCEECCCCCCCCHHHHHHHHHCCCCCCCCCCEEEECCCCCHH
EILARPLAELEAMAIDAALRLSDNNVTRAARLLDVSPSTIYRKHSAYKSGGTGGSAGR
HHHHHHHHHHHHHHHHHEEEECCCCHHHHHHEEECCCHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MHRILMVEDSPSNAELYATYLRASGMTVTLAGTGGQALDYLVADASAFDLVVLDLGLPDM
CCEEEEEECCCCCCCEEEEEEECCCCEEEEECCCCHHHHHHHCCCCCEEEEEEECCCCCC
DGLEILRKLGGPAAMPPTVVLTGSGSVRRAVEAMREGAIDFLVKPCGADKLRTAVEEALE
HHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHH
RRTPTAEGRAPAADPTRAKRGPSGFLGSSPAMSNVFSLIEAAAASSTASVFITGETGTGK
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCH
ELCANAIHNLSDRRNGPFVAINCGAIPRELMESEIFGHRKGAFTGAINDREGAASRADGG
HHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCC
TLFLDELCEMDLDLQVKLLRFIQTGTYRKVGDGADRQVNIRFVCATNRDPLVEVKAGRFR
EEEHHHHHCCCCCHHHHHHHHHHCCCCEECCCCCCCEEEEEEEEECCCCCEEEECCCCHH
EDLYYRLYVVPIHLPPLSARGDDIILLARRFLADFSKLEGKRFQEFSADALGRIMAYRWP
CCEEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHEEEECC
GNVRQLENVVRNIVVLNDGVTVEPTMLPPPLDCVEAVVSLPVDPQSATGAVVGGGTLSLD
CCHHHHHHHHHEEEEECCCCEECCCCCCCCHHHHHHHHHCCCCCCCCCCEEEECCCCCHH
EILARPLAELEAMAIDAALRLSDNNVTRAARLLDVSPSTIYRKHSAYKSGGTGGSAGR
HHHHHHHHHHHHHHHHHEEEECCCCHHHHHHEEECCCHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8065259 [H]