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The map label for this gene is potH [H]

Identifier: 83593381

GI number: 83593381

Start: 2365727

End: 2366947

Strand: Reverse

Name: potH [H]

Synonym: Rru_A2046

Alternate gene names: 83593381

Gene position: 2366947-2365727 (Counterclockwise)

Preceding gene: 83593382

Following gene: 83593380

Centisome position: 54.38

GC content: 65.36

Gene sequence:

>1221_bases
ATGGGCGCCGAGATGTCGCTGAAGTCCAAGCTGCGCCGCGCCGAACGCCTGCGGCAGTTGCGCGCCTTCGGTCTGATCCT
GCCCCTGTTGTTGTTCCTGGTCGTCACCTTCGTCGTGCCGATCGCCGATATGCTGCGGCGCTCGGTTCACGATCCCGAGT
TGTCCCAGGTGTGGCCGGCGACCACGAGCGCCATCAAGGGATGGACCGCGCGCGAGCGCCTGCCCGACGAGGCGGTTTTC
GCCGCCCTGGCCGGCGACCTGCGGGCCAGCCGCGACGCGGCGACGCTGGCGACGGCGGCGCGGCGTCTCAATTACGATTT
CAACGGATCGCGAACCCTGCTGTTCACCACGGCGCGCAAGCTGCCGGCGAGCGCGCCCTCGTGGACCGAGGCGCTGATCG
CCGCCGATCCGCGCTGGGGCGAGACCGAGGTCTGGGGGGCGATCAACCGGGCCTCGGGACCGCTTACGGCGTTTTTCCTG
ATGCAGGCCGTTGATCTGGAACGCGACGCCACTGGGGCCCTGCGCGGCGCTCCGACCGATGAGGCGATCTATATCGACGT
TCTCGGCCGCACCTTCGGCATCAGCCTGACCGTCACCGTGCTCTGCCTCCTGCTCGGCTTTCCGGTCGCCTATCTTTTGG
CCAATCTGCCGCCGCGTCAGGCCAATTTGTTGATGATCCTGGTGCTTTTGCCGTTCTGGACCTCGCTCCTGGTGCGCACG
GCCGCCTGGGTCGTGCTGCTCCAGGATCAGGGGCTGGTCAATAACCTGCTGATTTGGCTCGGCGTTATCGAAAAGCCGCT
GCGCTTGATGTACAACCGCGTCGGGGTGATCGTGGCGATGACCCATGTGTTGCTGCCCTTCATGATCTTGCCGCTCTATT
CGGTGATGAAGGGCATCTCGCCGGTTTATCTGCGCGCGGCGCGTTCGCTCGGCGCCACGCCGACCATCGCCTTCTTGCGG
GTTTATCTGCCGCAATGTCTGCCCGGCTTTGGCGCGGGCGCCCTGCTGACCTTCATTCTGGCTTTGGGTTATTACATCAC
GCCGGCCCTGGTCGGCGGCGCGGCCGATCAGATGCTCAGCTACTTCATCGCCTTCTATACCTCCGACACGGTGAACTGGG
GCATGGCCTCGGCGCTGGGGGCGGTCTTGCTGGCGGCGACCCTGGTGCTCTACGCCGTCTATACCCGGCTGGTCGGTACG
GATTCGGTGAAGCTGTCTTAA

Upstream 100 bases:

>100_bases
GGGCCGCCGTCCAGTAAGGACGCGCCCGCTCGGCGCCCGGGAACCCTGGGGAGGTGTTCCCGGGCGCACGGTTTGGGTTT
TCTAGGAAGGGGAGATGCCG

Downstream 100 bases:

>100_bases
AATCGCCAGGGGATTGTCTCCAAGTCCCTGGATCCCGGGAGGCCCGATCATGCGCTCTACCCATACCCGCACCGGCGAGG
AAAAGTTCTGGATCACCCTG

Product: binding-protein dependent transport system inner membrane protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MGAEMSLKSKLRRAERLRQLRAFGLILPLLLFLVVTFVVPIADMLRRSVHDPELSQVWPATTSAIKGWTARERLPDEAVF
AALAGDLRASRDAATLATAARRLNYDFNGSRTLLFTTARKLPASAPSWTEALIAADPRWGETEVWGAINRASGPLTAFFL
MQAVDLERDATGALRGAPTDEAIYIDVLGRTFGISLTVTVLCLLLGFPVAYLLANLPPRQANLLMILVLLPFWTSLLVRT
AAWVVLLQDQGLVNNLLIWLGVIEKPLRLMYNRVGVIVAMTHVLLPFMILPLYSVMKGISPVYLRAARSLGATPTIAFLR
VYLPQCLPGFGAGALLTFILALGYYITPALVGGAADQMLSYFIAFYTSDTVNWGMASALGAVLLAATLVLYAVYTRLVGT
DSVKLS

Sequences:

>Translated_406_residues
MGAEMSLKSKLRRAERLRQLRAFGLILPLLLFLVVTFVVPIADMLRRSVHDPELSQVWPATTSAIKGWTARERLPDEAVF
AALAGDLRASRDAATLATAARRLNYDFNGSRTLLFTTARKLPASAPSWTEALIAADPRWGETEVWGAINRASGPLTAFFL
MQAVDLERDATGALRGAPTDEAIYIDVLGRTFGISLTVTVLCLLLGFPVAYLLANLPPRQANLLMILVLLPFWTSLLVRT
AAWVVLLQDQGLVNNLLIWLGVIEKPLRLMYNRVGVIVAMTHVLLPFMILPLYSVMKGISPVYLRAARSLGATPTIAFLR
VYLPQCLPGFGAGALLTFILALGYYITPALVGGAADQMLSYFIAFYTSDTVNWGMASALGAVLLAATLVLYAVYTRLVGT
DSVKLS
>Mature_405_residues
GAEMSLKSKLRRAERLRQLRAFGLILPLLLFLVVTFVVPIADMLRRSVHDPELSQVWPATTSAIKGWTARERLPDEAVFA
ALAGDLRASRDAATLATAARRLNYDFNGSRTLLFTTARKLPASAPSWTEALIAADPRWGETEVWGAINRASGPLTAFFLM
QAVDLERDATGALRGAPTDEAIYIDVLGRTFGISLTVTVLCLLLGFPVAYLLANLPPRQANLLMILVLLPFWTSLLVRTA
AWVVLLQDQGLVNNLLIWLGVIEKPLRLMYNRVGVIVAMTHVLLPFMILPLYSVMKGISPVYLRAARSLGATPTIAFLRV
YLPQCLPGFGAGALLTFILALGYYITPALVGGAADQMLSYFIAFYTSDTVNWGMASALGAVLLAATLVLYAVYTRLVGTD
SVKLS

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=206, Percent_Identity=35.4368932038835, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI1787369, Length=216, Percent_Identity=35.1851851851852, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI1787713, Length=181, Percent_Identity=33.7016574585635, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 44242; Mature: 44111

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAEMSLKSKLRRAERLRQLRAFGLILPLLLFLVVTFVVPIADMLRRSVHDPELSQVWPA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCC
TTSAIKGWTARERLPDEAVFAALAGDLRASRDAATLATAARRLNYDFNGSRTLLFTTARK
HHHHHCCCHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEEEEEHHH
LPASAPSWTEALIAADPRWGETEVWGAINRASGPLTAFFLMQAVDLERDATGALRGAPTD
CCCCCCCHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCC
EAIYIDVLGRTFGISLTVTVLCLLLGFPVAYLLANLPPRQANLLMILVLLPFWTSLLVRT
CEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
AAWVVLLQDQGLVNNLLIWLGVIEKPLRLMYNRVGVIVAMTHVLLPFMILPLYSVMKGIS
HHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PVYLRAARSLGATPTIAFLRVYLPQCLPGFGAGALLTFILALGYYITPALVGGAADQMLS
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
YFIAFYTSDTVNWGMASALGAVLLAATLVLYAVYTRLVGTDSVKLS
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
GAEMSLKSKLRRAERLRQLRAFGLILPLLLFLVVTFVVPIADMLRRSVHDPELSQVWPA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCC
TTSAIKGWTARERLPDEAVFAALAGDLRASRDAATLATAARRLNYDFNGSRTLLFTTARK
HHHHHCCCHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEEEEEHHH
LPASAPSWTEALIAADPRWGETEVWGAINRASGPLTAFFLMQAVDLERDATGALRGAPTD
CCCCCCCHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCC
EAIYIDVLGRTFGISLTVTVLCLLLGFPVAYLLANLPPRQANLLMILVLLPFWTSLLVRT
CEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
AAWVVLLQDQGLVNNLLIWLGVIEKPLRLMYNRVGVIVAMTHVLLPFMILPLYSVMKGIS
HHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PVYLRAARSLGATPTIAFLRVYLPQCLPGFGAGALLTFILALGYYITPALVGGAADQMLS
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
YFIAFYTSDTVNWGMASALGAVLLAATLVLYAVYTRLVGTDSVKLS
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]