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The map label for this gene is lipB

Identifier: 83593331

GI number: 83593331

Start: 2306189

End: 2306839

Strand: Direct

Name: lipB

Synonym: Rru_A1996

Alternate gene names: 83593331

Gene position: 2306189-2306839 (Clockwise)

Preceding gene: 83593320

Following gene: 83593332

Centisome position: 52.98

GC content: 65.59

Gene sequence:

>651_bases
ATGGAGTGGCGTTTCGCCGATACCCCCGTTTCCTACCCCGAAGCCCTGGCCTTCATGGACGACCGCATCGCCAAGATCCG
CGAGGATCGGGCCGGGGAATGCGTCTGGCTGCTTGAACACCCCCCGCTTTATACGGCGGGGACCAGCGCCGATCCGGCCG
ACCTGCTCAGCCCCGATCGCTTTCCGGTCTATGCCGCCGGGCGCGGCGGCCAATACACCTATCATGGCCCCGGCCAACGC
GTCGGCTATGTGATGCTCGACCTGCGTCGACGCGGCAAGGACGTGCGCTGCTTCGTGCATAATCTGGAAGAGCTGATGAT
CCGCGCCCTGGCCGAGTTCAATATCCGGGGCGAGCGCCGCGAGGGCCGGGTCGGCATCTGGGTGGCGCGCGGCGGCGGAC
GCGAGGACAAGATCGGCGCCATCGGCGTGCGGGTCCGCCATTGGATCACCTTCCACGGCTTCGCCCTCAACGTCGAACCC
GATCTGTCGCATTTCTCGGGCATCGTGCCCTGCGGCATCAGCGACCACGGGGTGACCAGCCTGTGGGATCTGGGCCAGAC
GGCGACACTCCAAGAGGTTGATTCCGCTTTGATGCGCAGCTTTGATGCGGTATTTGGGCCCTGCCCGACGGAAAGCTTGA
CCTCGACGTGA

Upstream 100 bases:

>100_bases
CTTCTTGCCCTGCGAGGGCGGATGAAATAGCAAACCCCTTGGACCTCGGCAATCCCTTTTCTTTCACGCCTCGCACTTTT
TTTCGAAGGATCCCGTCGTT

Downstream 100 bases:

>100_bases
ACCAATCGCGCCAAATATGCGTAGTATAGGCCATGAAAGCGCCCTCTCCTCCTCTGCGCGCTCTGCTCATGACCGCGCTC
TCGCCGCTGTTCGCCGCCTG

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MEWRFADTPVSYPEALAFMDDRIAKIREDRAGECVWLLEHPPLYTAGTSADPADLLSPDRFPVYAAGRGGQYTYHGPGQR
VGYVMLDLRRRGKDVRCFVHNLEELMIRALAEFNIRGERREGRVGIWVARGGGREDKIGAIGVRVRHWITFHGFALNVEP
DLSHFSGIVPCGISDHGVTSLWDLGQTATLQEVDSALMRSFDAVFGPCPTESLTST

Sequences:

>Translated_216_residues
MEWRFADTPVSYPEALAFMDDRIAKIREDRAGECVWLLEHPPLYTAGTSADPADLLSPDRFPVYAAGRGGQYTYHGPGQR
VGYVMLDLRRRGKDVRCFVHNLEELMIRALAEFNIRGERREGRVGIWVARGGGREDKIGAIGVRVRHWITFHGFALNVEP
DLSHFSGIVPCGISDHGVTSLWDLGQTATLQEVDSALMRSFDAVFGPCPTESLTST
>Mature_216_residues
MEWRFADTPVSYPEALAFMDDRIAKIREDRAGECVWLLEHPPLYTAGTSADPADLLSPDRFPVYAAGRGGQYTYHGPGQR
VGYVMLDLRRRGKDVRCFVHNLEELMIRALAEFNIRGERREGRVGIWVARGGGREDKIGAIGVRVRHWITFHGFALNVEP
DLSHFSGIVPCGISDHGVTSLWDLGQTATLQEVDSALMRSFDAVFGPCPTESLTST

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=216, Percent_Identity=33.7962962962963, Blast_Score=76, Evalue=3e-14,
Organism=Escherichia coli, GI87081767, Length=189, Percent_Identity=37.037037037037, Blast_Score=127, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6323268, Length=211, Percent_Identity=26.5402843601896, Blast_Score=85, Evalue=8e-18,
Organism=Drosophila melanogaster, GI21355483, Length=175, Percent_Identity=36.5714285714286, Blast_Score=101, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_RHORT (Q2RSU9)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_427083.1
- ProteinModelPortal:   Q2RSU9
- SMR:   Q2RSU9
- STRING:   Q2RSU9
- GeneID:   3835421
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A1996
- NMPDR:   fig|1085.1.peg.2570
- eggNOG:   COG0321
- HOGENOM:   HBG716160
- OMA:   HPPVFTL
- PhylomeDB:   Q2RSU9
- ProtClustDB:   PRK14341
- BioCyc:   RRUB269796:RRU_A1996-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 24064; Mature: 24064

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 171-171

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEWRFADTPVSYPEALAFMDDRIAKIREDRAGECVWLLEHPPLYTAGTSADPADLLSPDR
CCCEECCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEECCCCCCHHHHCCCCC
FPVYAAGRGGQYTYHGPGQRVGYVMLDLRRRGKDVRCFVHNLEELMIRALAEFNIRGERR
CEEEEECCCCCEEECCCCCCHHHEEEEHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
EGRVGIWVARGGGREDKIGAIGVRVRHWITFHGFALNVEPDLSHFSGIVPCGISDHGVTS
CCCEEEEEECCCCCCCCEEEEEEEEEEEEEEEEEEEEECCCHHHHCCCCCCCCCCCCCHH
LWDLGQTATLQEVDSALMRSFDAVFGPCPTESLTST
HHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MEWRFADTPVSYPEALAFMDDRIAKIREDRAGECVWLLEHPPLYTAGTSADPADLLSPDR
CCCEECCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEECCCCCCHHHHCCCCC
FPVYAAGRGGQYTYHGPGQRVGYVMLDLRRRGKDVRCFVHNLEELMIRALAEFNIRGERR
CEEEEECCCCCEEECCCCCCHHHEEEEHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
EGRVGIWVARGGGREDKIGAIGVRVRHWITFHGFALNVEPDLSHFSGIVPCGISDHGVTS
CCCEEEEEECCCCCCCCEEEEEEEEEEEEEEEEEEEEECCCHHHHCCCCCCCCCCCCCHH
LWDLGQTATLQEVDSALMRSFDAVFGPCPTESLTST
HHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA