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The map label for this gene is phaZ1 [H]

Identifier: 83593304

GI number: 83593304

Start: 2276369

End: 2277490

Strand: Direct

Name: phaZ1 [H]

Synonym: Rru_A1969

Alternate gene names: 83593304

Gene position: 2276369-2277490 (Clockwise)

Preceding gene: 83593303

Following gene: 83593308

Centisome position: 52.3

GC content: 72.01

Gene sequence:

>1122_bases
ATGAAAAACCCCTTCGCCCACGGTTCCCTGCCCCCTATCCCCCTGCCCGATCTGACCGAAGCGACCCGCCTGACCCGCAG
CGGTCGGCTGGGCGAGGCCACGGCGCTGATCGGCCGCCTGCTTGGCCGGGGTCAGCCCGAGGCCACGCCACCCTTCGAGC
CGTCCCCCCTGACGCCGCCAACCATCGACGGCGAGGCGATCGAGATCACGCCCCGCCCCGGTGGCGCCCCCCGTCCCCAG
GCGCCGCCGGCCGGTCGCGGCGCCTTCCTGGCGCTGACCCATCGCGGCCCGGCCGGCACGCGTGATTACAAGCTGTTCGT
GCCGCCCGGCTCCGGGGACAGCTCCCTGCCGCTGATCCTCATGCTGCACGGCTGCACCCAGGACCCCGATGATTTCGCCG
CCGGCACCGGCATGAACAAGCTGGCCGAGGAGGCGGGCTGTCTGGTCGCCTATCCGCGTCAGCCGGCCTCGGCCAATCCG
TCGAAATGCTGGAACTGGTTCAATGACGAGAACCAGCATCGCGGCCAGGGCGAGGCCGCCCTGCTGGCCGAGCTGACCCG
GGCGATCATCGGCGAGCGCCGGGTCGATCCGGCGCGGGTTTATGTGGCGGGCTTATCGGCGGGCGGCGCGGCGGCGGCGA
TCCTGGCCGTCACCTACCCCGATCTTTTCGCCGCCGTCGGCGTGCATTCTGGTCTGGCCTGCGGCGCGGCGCGCGACATG
GCCTCAGCCTTCGCCGCCATGCGCCAGGGCGGCGCCACCGTCCGCCCCGCCCCCTCGGCGATCCCGGCGATCGTTTTCCA
CGGCGATCGCGATACCACCGTTCATCCGGGCAACGGCGATTCCCTGGTGTCGCAATTCACCGCCGACCGCAGCGGCCTGC
GCGAAACCAGCCAGACGGGTCGGGCGCCGGGCGGCCATGGCTATCGCCGCACCCTTCACAGCGCCGCCGACGGCACGGTG
CTGTGCGAGCAATGGACGATCCAGGGCGGTGGCCACGCCTGGTCGGGCGGTCAGCCGGCGGGATCGTTCACCGACCCCCG
GGGGCCCGACGCCAGCGCTGAAATGCTCCGCTTCTTCCTGGCGCAGTGCCATCCTGGACTGGGCGCCAAAGCCCCCTGCT
AA

Upstream 100 bases:

>100_bases
CCGTAGCGCCTGATCGCCTTTTGCCCCCCTTCGCGCCTTTCTCCGTTGACCCATGGCTTCCCGATCCTCGGGAAAGTCCC
CGCCCCAGGACTTCACGCCC

Downstream 100 bases:

>100_bases
AGAACAGCCACCGGGCGTTTGGTGATCCGCAGCACCAGGGCGGTGATCAGGGTGCCAACGACCAGGGCGACGATATAGCC
CAGCAAATGGGTGACGGCAT

Product: esterase, PHB depolymerase

Products: NA

Alternate protein names: PHA depolymerase; PHB depolymerase [H]

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MKNPFAHGSLPPIPLPDLTEATRLTRSGRLGEATALIGRLLGRGQPEATPPFEPSPLTPPTIDGEAIEITPRPGGAPRPQ
APPAGRGAFLALTHRGPAGTRDYKLFVPPGSGDSSLPLILMLHGCTQDPDDFAAGTGMNKLAEEAGCLVAYPRQPASANP
SKCWNWFNDENQHRGQGEAALLAELTRAIIGERRVDPARVYVAGLSAGGAAAAILAVTYPDLFAAVGVHSGLACGAARDM
ASAFAAMRQGGATVRPAPSAIPAIVFHGDRDTTVHPGNGDSLVSQFTADRSGLRETSQTGRAPGGHGYRRTLHSAADGTV
LCEQWTIQGGGHAWSGGQPAGSFTDPRGPDASAEMLRFFLAQCHPGLGAKAPC

Sequences:

>Translated_373_residues
MKNPFAHGSLPPIPLPDLTEATRLTRSGRLGEATALIGRLLGRGQPEATPPFEPSPLTPPTIDGEAIEITPRPGGAPRPQ
APPAGRGAFLALTHRGPAGTRDYKLFVPPGSGDSSLPLILMLHGCTQDPDDFAAGTGMNKLAEEAGCLVAYPRQPASANP
SKCWNWFNDENQHRGQGEAALLAELTRAIIGERRVDPARVYVAGLSAGGAAAAILAVTYPDLFAAVGVHSGLACGAARDM
ASAFAAMRQGGATVRPAPSAIPAIVFHGDRDTTVHPGNGDSLVSQFTADRSGLRETSQTGRAPGGHGYRRTLHSAADGTV
LCEQWTIQGGGHAWSGGQPAGSFTDPRGPDASAEMLRFFLAQCHPGLGAKAPC
>Mature_373_residues
MKNPFAHGSLPPIPLPDLTEATRLTRSGRLGEATALIGRLLGRGQPEATPPFEPSPLTPPTIDGEAIEITPRPGGAPRPQ
APPAGRGAFLALTHRGPAGTRDYKLFVPPGSGDSSLPLILMLHGCTQDPDDFAAGTGMNKLAEEAGCLVAYPRQPASANP
SKCWNWFNDENQHRGQGEAALLAELTRAIIGERRVDPARVYVAGLSAGGAAAAILAVTYPDLFAAVGVHSGLACGAARDM
ASAFAAMRQGGATVRPAPSAIPAIVFHGDRDTTVHPGNGDSLVSQFTADRSGLRETSQTGRAPGGHGYRRTLHSAADGTV
LCEQWTIQGGGHAWSGGQPAGSFTDPRGPDASAEMLRFFLAQCHPGLGAKAPC

Specific function: Specific for poly(hydroxyalkanoic acid) consisting of monomers of four or five carbon atoms and for P- nitrophenylbutyrate as substrates [H]

COG id: COG3509

COG function: function code Q; Poly(3-hydroxybutyrate) depolymerase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Lipase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010126 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 38615; Mature: 38615

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNPFAHGSLPPIPLPDLTEATRLTRSGRLGEATALIGRLLGRGQPEATPPFEPSPLTPP
CCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
TIDGEAIEITPRPGGAPRPQAPPAGRGAFLALTHRGPAGTRDYKLFVPPGSGDSSLPLIL
CCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEECCCCCCCCCCEEE
MLHGCTQDPDDFAAGTGMNKLAEEAGCLVAYPRQPASANPSKCWNWFNDENQHRGQGEAA
EEECCCCCHHHHHHCCCHHHHHHHCCCEEEECCCCCCCCHHHHHCCCCCCCCCCCCCHHH
LLAELTRAIIGERRVDPARVYVAGLSAGGAAAAILAVTYPDLFAAVGVHSGLACGAARDM
HHHHHHHHHHCCCCCCCEEEEEEECCCCCHHEEEEEEHHHHHHHHHHHHCCHHHHHHHHH
ASAFAAMRQGGATVRPAPSAIPAIVFHGDRDTTVHPGNGDSLVSQFTADRSGLRETSQTG
HHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHC
RAPGGHGYRRTLHSAADGTVLCEQWTIQGGGHAWSGGQPAGSFTDPRGPDASAEMLRFFL
CCCCCCHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
AQCHPGLGAKAPC
HHCCCCCCCCCCC
>Mature Secondary Structure
MKNPFAHGSLPPIPLPDLTEATRLTRSGRLGEATALIGRLLGRGQPEATPPFEPSPLTPP
CCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
TIDGEAIEITPRPGGAPRPQAPPAGRGAFLALTHRGPAGTRDYKLFVPPGSGDSSLPLIL
CCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEECCCCCCCCCCEEE
MLHGCTQDPDDFAAGTGMNKLAEEAGCLVAYPRQPASANPSKCWNWFNDENQHRGQGEAA
EEECCCCCHHHHHHCCCHHHHHHHCCCEEEECCCCCCCCHHHHHCCCCCCCCCCCCCHHH
LLAELTRAIIGERRVDPARVYVAGLSAGGAAAAILAVTYPDLFAAVGVHSGLACGAARDM
HHHHHHHHHHCCCCCCCEEEEEEECCCCCHHEEEEEEHHHHHHHHHHHHCCHHHHHHHHH
ASAFAAMRQGGATVRPAPSAIPAIVFHGDRDTTVHPGNGDSLVSQFTADRSGLRETSQTG
HHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHC
RAPGGHGYRRTLHSAADGTVLCEQWTIQGGGHAWSGGQPAGSFTDPRGPDASAEMLRFFL
CCCCCCHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
AQCHPGLGAKAPC
HHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8269961 [H]