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The map label for this gene is lexA

Identifier: 83593233

GI number: 83593233

Start: 2199791

End: 2200546

Strand: Direct

Name: lexA

Synonym: Rru_A1898

Alternate gene names: 83593233

Gene position: 2199791-2200546 (Clockwise)

Preceding gene: 83593232

Following gene: 83593235

Centisome position: 50.54

GC content: 65.48

Gene sequence:

>756_bases
ATGCTCACGCGCAAGCAGTACCTGCTGTTGTCGTTCATCGACCAGCGTTTGAAGCTTTCGGGCGTTTCCCCCTCGTTCGA
CGAGATGAAGGACGCCCTGGGGCTCAAGTCCAAATCGGGCATTCACCGCCTGATCAAAGGGCTGGAGGAACGGGGCTTTC
TCAAGCGCCTTCCCCATCGGGCCCGCGCCCTGGAAGTGCTGCGCCTGCCCTGCAATCTGACCTTCGACGGCGACGGCGAA
GCCCTTGACGACGGGCAGCCCGCGCCGCTGTTCGGCGGCCCTCTGCCGGAAACCGGATTCTCGCCCCAGGTCATCCGCGG
CAATTTCACCCCGACCCTGCCGTCGACCCAAGTACCGCAGGTGCTGTTCACCGAGTCGATCAGCCTGCCGCTTTTGGGCA
AGATCGCCGCCGGCACGCCGATCGCCGCCCTGATCGATCCGACGTCGAGCATCGATGTGCCGGCCAGCATGGTGCGCGGC
GGCGAGCATTTCGCCCTGCGCATCGAAGGGGACTCGATGATCGAAGCGGGCATCCTCAGTGGTGATCTGGCGGTGGTGCG
GCGCTGCGACGAAGCCGAAAACGGCACGGTCATCGTCGCCCTGGTCGATAACGAGGAAGCCACCCTGAAGCGCCTGCGCC
GCAAGGGGGCCTCGATCGCCCTGGAACCGGCCAACCGCGCCTTCAAAACCCAGATCTACGGCCCCGACCGCGTGCGCATC
CAGGGTCGGCTGGTCGGGCTGATCCGCAGCTATTAG

Upstream 100 bases:

>100_bases
CTTCCAGGCGGTATTCACGTTTGGTTCTTCTTGTCGCCCGCTCAGAGGGTGGGCGCGCAGAGGTTGAGGGGCCGGGCAAA
AGGCTGGGAGTCGCTCTGGC

Downstream 100 bases:

>100_bases
GGCAAATCCGTCTTCGCAAAAAAAAGATCCCCGCCGCTCGGCGCCGGGGATCTTTTGGCGATCAGGCCTTGGCGAACAAT
AACCGTTCGGCCGTGCGTTC

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MLTRKQYLLLSFIDQRLKLSGVSPSFDEMKDALGLKSKSGIHRLIKGLEERGFLKRLPHRARALEVLRLPCNLTFDGDGE
ALDDGQPAPLFGGPLPETGFSPQVIRGNFTPTLPSTQVPQVLFTESISLPLLGKIAAGTPIAALIDPTSSIDVPASMVRG
GEHFALRIEGDSMIEAGILSGDLAVVRRCDEAENGTVIVALVDNEEATLKRLRRKGASIALEPANRAFKTQIYGPDRVRI
QGRLVGLIRSY

Sequences:

>Translated_251_residues
MLTRKQYLLLSFIDQRLKLSGVSPSFDEMKDALGLKSKSGIHRLIKGLEERGFLKRLPHRARALEVLRLPCNLTFDGDGE
ALDDGQPAPLFGGPLPETGFSPQVIRGNFTPTLPSTQVPQVLFTESISLPLLGKIAAGTPIAALIDPTSSIDVPASMVRG
GEHFALRIEGDSMIEAGILSGDLAVVRRCDEAENGTVIVALVDNEEATLKRLRRKGASIALEPANRAFKTQIYGPDRVRI
QGRLVGLIRSY
>Mature_251_residues
MLTRKQYLLLSFIDQRLKLSGVSPSFDEMKDALGLKSKSGIHRLIKGLEERGFLKRLPHRARALEVLRLPCNLTFDGDGE
ALDDGQPAPLFGGPLPETGFSPQVIRGNFTPTLPSTQVPQVLFTESISLPLLGKIAAGTPIAALIDPTSSIDVPASMVRG
GEHFALRIEGDSMIEAGILSGDLAVVRRCDEAENGTVIVALVDNEEATLKRLRRKGASIALEPANRAFKTQIYGPDRVRI
QGRLVGLIRSY

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family

Homologues:

Organism=Escherichia coli, GI1790476, Length=133, Percent_Identity=36.0902255639098, Blast_Score=79, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEXA_RHORT (Q2RT47)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_426985.1
- ProteinModelPortal:   Q2RT47
- SMR:   Q2RT47
- STRING:   Q2RT47
- MEROPS:   S24.001
- GeneID:   3835322
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A1898
- NMPDR:   fig|1085.1.peg.2471
- eggNOG:   COG1974
- HOGENOM:   HBG679610
- OMA:   LEPANAR
- PhylomeDB:   Q2RT47
- ProtClustDB:   PRK00215
- BioCyc:   RRUB269796:RRU_A1898-MONOMER
- GO:   GO:0006508
- GO:   GO:0006350
- HAMAP:   MF_00015
- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991
- Gene3D:   G3DSA:2.10.109.10
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00726
- TIGRFAMs:   TIGR00498

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.88

Molecular weight: Translated: 27250; Mature: 27250

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: NA

Important sites: ACT_SITE 172-172 ACT_SITE 210-210

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTRKQYLLLSFIDQRLKLSGVSPSFDEMKDALGLKSKSGIHRLIKGLEERGFLKRLPHR
CCCCHHHHHHHHHHHHHEECCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHCCHH
ARALEVLRLPCNLTFDGDGEALDDGQPAPLFGGPLPETGFSPQVIRGNFTPTLPSTQVPQ
HHHHHHEECCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCC
VLFTESISLPLLGKIAAGTPIAALIDPTSSIDVPASMVRGGEHFALRIEGDSMIEAGILS
EEECCCCCCCCHHHHCCCCCEEEEECCCCCCCCCHHHHCCCCEEEEEECCCCEEEHHHCC
GDLAVVRRCDEAENGTVIVALVDNEEATLKRLRRKGASIALEPANRAFKTQIYGPDRVRI
CCHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHCCCCEEECCCCCEEEEEECCCCEEEE
QGRLVGLIRSY
CCEEEEHHHCC
>Mature Secondary Structure
MLTRKQYLLLSFIDQRLKLSGVSPSFDEMKDALGLKSKSGIHRLIKGLEERGFLKRLPHR
CCCCHHHHHHHHHHHHHEECCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHCCHH
ARALEVLRLPCNLTFDGDGEALDDGQPAPLFGGPLPETGFSPQVIRGNFTPTLPSTQVPQ
HHHHHHEECCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCC
VLFTESISLPLLGKIAAGTPIAALIDPTSSIDVPASMVRGGEHFALRIEGDSMIEAGILS
EEECCCCCCCCHHHHCCCCCEEEEECCCCCCCCCHHHHCCCCEEEEEECCCCEEEHHHCC
GDLAVVRRCDEAENGTVIVALVDNEEATLKRLRRKGASIALEPANRAFKTQIYGPDRVRI
CCHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHCCCCEEECCCCCEEEEEECCCCEEEE
QGRLVGLIRSY
CCEEEEHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA