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The map label for this gene is pyrG

Identifier: 83593222

GI number: 83593222

Start: 2185815

End: 2187443

Strand: Reverse

Name: pyrG

Synonym: Rru_A1887

Alternate gene names: 83593222

Gene position: 2187443-2185815 (Counterclockwise)

Preceding gene: 83593223

Following gene: 83593221

Centisome position: 50.25

GC content: 62.86

Gene sequence:

>1629_bases
ATGACTCGTTTCATCTTTATCACCGGCGGTGTGGTTTCTTCCCTGGGCAAGGGCTTGGCTTCGGCGGCGCTTGGCGCCCT
GTTGCAGGCGCGCGGCTATAAGGTTCGGCTGCGCAAGCTTGATCCCTACCTCAACGTCGATCCCGGCACGATGAGCCCGA
CCCAGCATGGCGAGGTCTTCGTTACCGATGACGGGGCGGAAACCGACCTTGATCTGGGCCATTACGAGCGCTTCACCGGC
GTTTCGGCCCGGCGCAGCGACAATGTGACGACGGGGCGCATCTATTCCGACGTCATCGCCCGCGAGCGTCGCGGCGATTA
TCTGGGCAAGACCGTTCAGGTCATTCCCCATGTGACCGACGCCATCAAGGAATTCGCCACCGCCGACCTCACCGACGAGG
ATTTCGTGCTGTGCGAGATCGGCGGCACGGTCGGCGATATCGAAAGCCTGCCCTTCCTCGAGGCGATCCGCCAGCTCCGC
AACGAGATGGGGGCCGAGCGCAGCCTGATCATTCACCTGACGCTGCTGCCCTATATCCCCTCGGCCGGCGAATTGAAGAC
CAAGCCGACCCAGCATTCGGTCAAGGAGCTGCTGGGTCTGGGCATTCAGCCCGATCTTTTGATGTGCCGCTCCGACCGGC
CGATCCCGGAAGCCGAGCGCAACAAGATCTCGCTGTTTTGCAACGTGCGGCGCGAGGCGGTGATCCCGGCCCTTGACGTC
GACACCATCTATCAGGTGCCCGTCAGCTACCACGCCCAGGGTCTTGACGTTCAGGTCTGCCGCCATTTCCACCTGCCGAT
GAACGAGGATCTCGACCTCACGCGCTGGCAGACCATCGTCGATCGCGTTCGCCATCCCGAAGGCGAGGTGACCATCGCCG
TCGTCGGCAAGTATACCAGCCTGATCGACAGCTATAAGTCGCTGGCCGAGGCCCTGGTCCATGGCGGCATCGCCAATGCG
GTCAAGGTCAAGCTGGTCTGGCTCGACAGCGAGATCTTCGAACGCGAGGACGCCGTTCATTATCTGGAAGGCGTCAACGG
CATTCTGGTGCCCGGCGGCTTCGGCGAGCGCGGCTCGGAAGGCAAGATCGAAGCGGCGCGCTTCGCCCGCGAGCGCTCCG
TGCCTTATTTCGGCATCTGCTTTGGCATGCAGATGGCGGTGATCGAATTCGCCCGCCATGTCGCCGGTCTCCCGGGGGCC
AGTTCCTCGGAATTCGGCGTTCCCGAGGTGCCGTTGGTCGGCTTGATGACCGAATGGGTGCGCGGCAACGAACGCGTGGC
GCGCGGCTCGGGCGATGACCTGGGCGGCACCATGCGGCTGGGCGCCTATGAATGCCATCTGCGGGCCCTGAGCAAGGTGC
GCGAGATCTATGGCGCCGAGGTGATCCACGAGCGTCACCGCCATCGCTATGAGGTCAATGTCACCTTCAAGGACCAGCTT
GAGGAAAAGGGGCTGGCGTTTTCCGGCATGTCGCCCGATGGGGTGTTGCCCGAGATCGTCGAGCTTCCCGATCATCCCTG
GTTCATCGGCGTGCAGTTCCACCCCGAACTGAAATCCAAGCCCTTCGATCCCCATCCGCTGTTCACATCCTTTGTCCAGG
CGGCGATTACCCAATCCCGCCTGGTCTGA

Upstream 100 bases:

>100_bases
CCGGCTTCGTCCGGCGGAATGAAAGGGGTCCAGGTCCGTCGGCCAAGGGTCGACGGACTGGGTCTGATGTTGTTTTTTGG
TCAGCGGGCACGACACGCGC

Downstream 100 bases:

>100_bases
TCCGCCCCCGCCGACGCGGGGCCCGGGCGGGCGCCGCGCGGTTGCCACGAGAGGTCTTCCCCCGCATGACTCCCCGCCAC
GTCGCCGTTGGCCCCGTCAC

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase

Number of amino acids: Translated: 542; Mature: 541

Protein sequence:

>542_residues
MTRFIFITGGVVSSLGKGLASAALGALLQARGYKVRLRKLDPYLNVDPGTMSPTQHGEVFVTDDGAETDLDLGHYERFTG
VSARRSDNVTTGRIYSDVIARERRGDYLGKTVQVIPHVTDAIKEFATADLTDEDFVLCEIGGTVGDIESLPFLEAIRQLR
NEMGAERSLIIHLTLLPYIPSAGELKTKPTQHSVKELLGLGIQPDLLMCRSDRPIPEAERNKISLFCNVRREAVIPALDV
DTIYQVPVSYHAQGLDVQVCRHFHLPMNEDLDLTRWQTIVDRVRHPEGEVTIAVVGKYTSLIDSYKSLAEALVHGGIANA
VKVKLVWLDSEIFEREDAVHYLEGVNGILVPGGFGERGSEGKIEAARFARERSVPYFGICFGMQMAVIEFARHVAGLPGA
SSSEFGVPEVPLVGLMTEWVRGNERVARGSGDDLGGTMRLGAYECHLRALSKVREIYGAEVIHERHRHRYEVNVTFKDQL
EEKGLAFSGMSPDGVLPEIVELPDHPWFIGVQFHPELKSKPFDPHPLFTSFVQAAITQSRLV

Sequences:

>Translated_542_residues
MTRFIFITGGVVSSLGKGLASAALGALLQARGYKVRLRKLDPYLNVDPGTMSPTQHGEVFVTDDGAETDLDLGHYERFTG
VSARRSDNVTTGRIYSDVIARERRGDYLGKTVQVIPHVTDAIKEFATADLTDEDFVLCEIGGTVGDIESLPFLEAIRQLR
NEMGAERSLIIHLTLLPYIPSAGELKTKPTQHSVKELLGLGIQPDLLMCRSDRPIPEAERNKISLFCNVRREAVIPALDV
DTIYQVPVSYHAQGLDVQVCRHFHLPMNEDLDLTRWQTIVDRVRHPEGEVTIAVVGKYTSLIDSYKSLAEALVHGGIANA
VKVKLVWLDSEIFEREDAVHYLEGVNGILVPGGFGERGSEGKIEAARFARERSVPYFGICFGMQMAVIEFARHVAGLPGA
SSSEFGVPEVPLVGLMTEWVRGNERVARGSGDDLGGTMRLGAYECHLRALSKVREIYGAEVIHERHRHRYEVNVTFKDQL
EEKGLAFSGMSPDGVLPEIVELPDHPWFIGVQFHPELKSKPFDPHPLFTSFVQAAITQSRLV
>Mature_541_residues
TRFIFITGGVVSSLGKGLASAALGALLQARGYKVRLRKLDPYLNVDPGTMSPTQHGEVFVTDDGAETDLDLGHYERFTGV
SARRSDNVTTGRIYSDVIARERRGDYLGKTVQVIPHVTDAIKEFATADLTDEDFVLCEIGGTVGDIESLPFLEAIRQLRN
EMGAERSLIIHLTLLPYIPSAGELKTKPTQHSVKELLGLGIQPDLLMCRSDRPIPEAERNKISLFCNVRREAVIPALDVD
TIYQVPVSYHAQGLDVQVCRHFHLPMNEDLDLTRWQTIVDRVRHPEGEVTIAVVGKYTSLIDSYKSLAEALVHGGIANAV
KVKLVWLDSEIFEREDAVHYLEGVNGILVPGGFGERGSEGKIEAARFARERSVPYFGICFGMQMAVIEFARHVAGLPGAS
SSEFGVPEVPLVGLMTEWVRGNERVARGSGDDLGGTMRLGAYECHLRALSKVREIYGAEVIHERHRHRYEVNVTFKDQLE
EKGLAFSGMSPDGVLPEIVELPDHPWFIGVQFHPELKSKPFDPHPLFTSFVQAAITQSRLV

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI28559085, Length=557, Percent_Identity=45.2423698384201, Blast_Score=475, Evalue=1e-134,
Organism=Homo sapiens, GI28559083, Length=557, Percent_Identity=45.2423698384201, Blast_Score=475, Evalue=1e-134,
Organism=Homo sapiens, GI221316689, Length=557, Percent_Identity=45.2423698384201, Blast_Score=475, Evalue=1e-134,
Organism=Homo sapiens, GI148491070, Length=557, Percent_Identity=45.0628366247756, Blast_Score=467, Evalue=1e-131,
Organism=Escherichia coli, GI1789142, Length=537, Percent_Identity=59.0316573556797, Blast_Score=617, Evalue=1e-178,
Organism=Caenorhabditis elegans, GI25148299, Length=614, Percent_Identity=38.1107491856677, Blast_Score=396, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6322563, Length=569, Percent_Identity=42.3550087873462, Blast_Score=440, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6319432, Length=572, Percent_Identity=40.5594405594406, Blast_Score=425, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24664469, Length=561, Percent_Identity=43.6720142602496, Blast_Score=439, Evalue=1e-123,
Organism=Drosophila melanogaster, GI21357815, Length=506, Percent_Identity=43.2806324110672, Blast_Score=379, Evalue=1e-105,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRG_RHORT (Q2RT58)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_426974.1
- HSSP:   P17812
- ProteinModelPortal:   Q2RT58
- SMR:   Q2RT58
- STRING:   Q2RT58
- GeneID:   3835311
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A1887
- NMPDR:   fig|1085.1.peg.2460
- eggNOG:   COG0504
- HOGENOM:   HBG597806
- OMA:   TEWLRGN
- PhylomeDB:   Q2RT58
- ProtClustDB:   PRK05380
- BioCyc:   RRUB269796:RRU_A1887-MONOMER
- HAMAP:   MF_01227
- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991
- TIGRFAMs:   TIGR00337

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase

EC number: =6.3.4.2

Molecular weight: Translated: 60018; Mature: 59887

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 380-380 ACT_SITE 514-514 ACT_SITE 516-516

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRFIFITGGVVSSLGKGLASAALGALLQARGYKVRLRKLDPYLNVDPGTMSPTQHGEVF
CCEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEECCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFTGVSARRSDNVTTGRIYSDVIARERRGDYLGKTVQVIPHVTD
EECCCCCCCCCCCHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHCCCHHCCHHHHHHHHHH
AIKEFATADLTDEDFVLCEIGGTVGDIESLPFLEAIRQLRNEMGAERSLIIHLTLLPYIP
HHHHHHCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCC
SAGELKTKPTQHSVKELLGLGIQPDLLMCRSDRPIPEAERNKISLFCNVRREAVIPALDV
CCCCCCCCCCHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCEEEEEEECCHHHCCCCCCC
DTIYQVPVSYHAQGLDVQVCRHFHLPMNEDLDLTRWQTIVDRVRHPEGEVTIAVVGKYTS
CHHEECCHHHCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECHHHH
LIDSYKSLAEALVHGGIANAVKVKLVWLDSEIFEREDAVHYLEGVNGILVPGGFGERGSE
HHHHHHHHHHHHHHCCCCCEEEEEEEEECHHHHHHHHHHHHHHCCCEEEECCCCCCCCCC
GKIEAARFARERSVPYFGICFGMQMAVIEFARHVAGLPGASSSEFGVPEVPLVGLMTEWV
CCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHH
RGNERVARGSGDDLGGTMRLGAYECHLRALSKVREIYGAEVIHERHRHRYEVNVTFKDQL
CCCCEEECCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEHHHH
EEKGLAFSGMSPDGVLPEIVELPDHPWFIGVQFHPELKSKPFDPHPLFTSFVQAAITQSR
HHCCCEECCCCCCCCCHHHHHCCCCCEEEEEEECCHHCCCCCCCCHHHHHHHHHHHHHHC
LV
CC
>Mature Secondary Structure 
TRFIFITGGVVSSLGKGLASAALGALLQARGYKVRLRKLDPYLNVDPGTMSPTQHGEVF
CEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEECCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFTGVSARRSDNVTTGRIYSDVIARERRGDYLGKTVQVIPHVTD
EECCCCCCCCCCCHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHCCCHHCCHHHHHHHHHH
AIKEFATADLTDEDFVLCEIGGTVGDIESLPFLEAIRQLRNEMGAERSLIIHLTLLPYIP
HHHHHHCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCC
SAGELKTKPTQHSVKELLGLGIQPDLLMCRSDRPIPEAERNKISLFCNVRREAVIPALDV
CCCCCCCCCCHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCEEEEEEECCHHHCCCCCCC
DTIYQVPVSYHAQGLDVQVCRHFHLPMNEDLDLTRWQTIVDRVRHPEGEVTIAVVGKYTS
CHHEECCHHHCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECHHHH
LIDSYKSLAEALVHGGIANAVKVKLVWLDSEIFEREDAVHYLEGVNGILVPGGFGERGSE
HHHHHHHHHHHHHHCCCCCEEEEEEEEECHHHHHHHHHHHHHHCCCEEEECCCCCCCCCC
GKIEAARFARERSVPYFGICFGMQMAVIEFARHVAGLPGASSSEFGVPEVPLVGLMTEWV
CCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHH
RGNERVARGSGDDLGGTMRLGAYECHLRALSKVREIYGAEVIHERHRHRYEVNVTFKDQL
CCCCEEECCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEHHHH
EEKGLAFSGMSPDGVLPEIVELPDHPWFIGVQFHPELKSKPFDPHPLFTSFVQAAITQSR
HHCCCEECCCCCCCCCHHHHHCCCCCEEEEEEECCHHCCCCCCCCHHHHHHHHHHHHHHC
LV
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA