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The map label for this gene is mdeA [H]

Identifier: 83593171

GI number: 83593171

Start: 2133380

End: 2134672

Strand: Direct

Name: mdeA [H]

Synonym: Rru_A1836

Alternate gene names: 83593171

Gene position: 2133380-2134672 (Clockwise)

Preceding gene: 83593164

Following gene: 83593172

Centisome position: 49.01

GC content: 65.2

Gene sequence:

>1293_bases
ATGGCGACCGACAGCACCCATCCCGAAACCCTGGTTCTTCACGCCGGATACCGCAGCGACGAGGCCACCAACGCGGTCGC
CGTGCCGATCTACCAAACCACCTCCTATCAGTTCCGCGACACCGAACAGGCGGCCAACCTGTTCGCGCTGAAGGAACTGG
GCAATATCTACACCCGCATCATGAACCCGACCTGCGACGCCCTTGAGCAGCGGGTGGCCGCCCTTGAAGGCGGCGTCGCC
GCCCTCGCCGTTTCCTCGGGGCAGACGGCGAGCACCTTCGCCGTGCTCAACCTGTGTCAGGCCGGCGATAATTTCGTAAC
CTCGACCGACCTCTATGGCGGCACCTGGAACCTGTTTGCCAATACCTTCAAGCAGATGGGCATCGAGGCGCGCTTCGTCG
ATCCCAGCGATCCCGAGGCCTTCCGCCGGGCGACCGACGAAAAAACCCGCTGCTATTACGCCGAGACCCTGCCCAATCCC
AAACTGCACGTCTTCCCGATCGCCGAGGTCGCCGCCATCGGGCGGGCCCTGGGCGTGCCGCTGATCGTCGACAACACCGC
CGCCCCCGTGCTGTGCCGGCCGCTGGCCCTGGGCGCCGCCGTCGTCGTCTATTCGGCGACCAAATACATCGGCGGCCATG
GCACCAGCATCGGCGGCATCGTCGTCGATGGCGGCAATTTCGACTGGGAGGCCCATGGGGCGCGCTTCCCCACCCTGACC
AACCCCGACCCCAGCTATCACGGCGCGGTCTGGACCGAAGCGGTCAAACCCCTGGGGCCGATCGCCTATATCTTGCGCAT
GCGGGTGATCTTGCTGCGCGACATCGGCGCCGCGCTCAGCCCGTTCAACGCCTTTTTGTTGATCCAGGGGCTGGAGACCC
TGCCCTTGCGCATGCGCGCCCATTGCGAAAACGCCCTCAAGGTCGTCGATTTCCTCAAATCCCACCCCCAGGTCACCACC
GTCATCCACCCCAGCGTCCAGACCGGCGAGTTGGCGCGGCGGCGCGACGGGGCGCTTGCCCACGGCCAGGGCGCCCTGGT
CGGCTTTGAACTGAAGGGCGGGCTGGAGGCCGGGCGCGCCTTCATCGACGCCCTGAAGATGCTCTACCATGTCGCCAATA
TCGGCGATGCCCGCAGTCTGGCCATTCACCCCGCCAGCACCACCCACTCGCAGTTGTCGGCCGAAGACCAGCTTGCAACC
GGCGTCTCGCAGGGGTATGTCCGTCTTTCCATCGGCATCGAGCATGCCGACGATATCATTGGTGACCTGAAACAGGCCTT
GGAGTCGTTGTGA

Upstream 100 bases:

>100_bases
AACAGTCTTTGATCTTGAATTCGGCCGGCGCCAGGGGTTGAGTGAAGGGCCCCCGCGCCTTGCGGCGGGCGATCCGTTCC
GTTACGCGAAAGGCCGATCC

Downstream 100 bases:

>100_bases
GCATCACCATTCCCAATCTGGGCAGCTTTCCCCGTACCCGCCTGCGGCGTCCGCGCCGGGACGATTGGTCCCGTCGCCTG
CTCGCCGAAAACACCTTGAC

Product: O-acetylhomoserine/O-acetylserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MATDSTHPETLVLHAGYRSDEATNAVAVPIYQTTSYQFRDTEQAANLFALKELGNIYTRIMNPTCDALEQRVAALEGGVA
ALAVSSGQTASTFAVLNLCQAGDNFVTSTDLYGGTWNLFANTFKQMGIEARFVDPSDPEAFRRATDEKTRCYYAETLPNP
KLHVFPIAEVAAIGRALGVPLIVDNTAAPVLCRPLALGAAVVVYSATKYIGGHGTSIGGIVVDGGNFDWEAHGARFPTLT
NPDPSYHGAVWTEAVKPLGPIAYILRMRVILLRDIGAALSPFNAFLLIQGLETLPLRMRAHCENALKVVDFLKSHPQVTT
VIHPSVQTGELARRRDGALAHGQGALVGFELKGGLEAGRAFIDALKMLYHVANIGDARSLAIHPASTTHSQLSAEDQLAT
GVSQGYVRLSIGIEHADDIIGDLKQALESL

Sequences:

>Translated_430_residues
MATDSTHPETLVLHAGYRSDEATNAVAVPIYQTTSYQFRDTEQAANLFALKELGNIYTRIMNPTCDALEQRVAALEGGVA
ALAVSSGQTASTFAVLNLCQAGDNFVTSTDLYGGTWNLFANTFKQMGIEARFVDPSDPEAFRRATDEKTRCYYAETLPNP
KLHVFPIAEVAAIGRALGVPLIVDNTAAPVLCRPLALGAAVVVYSATKYIGGHGTSIGGIVVDGGNFDWEAHGARFPTLT
NPDPSYHGAVWTEAVKPLGPIAYILRMRVILLRDIGAALSPFNAFLLIQGLETLPLRMRAHCENALKVVDFLKSHPQVTT
VIHPSVQTGELARRRDGALAHGQGALVGFELKGGLEAGRAFIDALKMLYHVANIGDARSLAIHPASTTHSQLSAEDQLAT
GVSQGYVRLSIGIEHADDIIGDLKQALESL
>Mature_429_residues
ATDSTHPETLVLHAGYRSDEATNAVAVPIYQTTSYQFRDTEQAANLFALKELGNIYTRIMNPTCDALEQRVAALEGGVAA
LAVSSGQTASTFAVLNLCQAGDNFVTSTDLYGGTWNLFANTFKQMGIEARFVDPSDPEAFRRATDEKTRCYYAETLPNPK
LHVFPIAEVAAIGRALGVPLIVDNTAAPVLCRPLALGAAVVVYSATKYIGGHGTSIGGIVVDGGNFDWEAHGARFPTLTN
PDPSYHGAVWTEAVKPLGPIAYILRMRVILLRDIGAALSPFNAFLLIQGLETLPLRMRAHCENALKVVDFLKSHPQVTTV
IHPSVQTGELARRRDGALAHGQGALVGFELKGGLEAGRAFIDALKMLYHVANIGDARSLAIHPASTTHSQLSAEDQLATG
VSQGYVRLSIGIEHADDIIGDLKQALESL

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=429, Percent_Identity=32.6340326340326, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI25453487, Length=427, Percent_Identity=29.2740046838407, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI299473759, Length=429, Percent_Identity=29.8368298368298, Blast_Score=134, Evalue=1e-31,
Organism=Escherichia coli, GI1790375, Length=424, Percent_Identity=30.6603773584906, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1789383, Length=426, Percent_Identity=25.3521126760563, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17538055, Length=427, Percent_Identity=31.8501170960187, Blast_Score=186, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17560056, Length=433, Percent_Identity=32.3325635103926, Blast_Score=180, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI71980708, Length=418, Percent_Identity=28.4688995215311, Blast_Score=173, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6323333, Length=439, Percent_Identity=44.4191343963554, Blast_Score=338, Evalue=1e-93,
Organism=Saccharomyces cerevisiae, GI6319307, Length=434, Percent_Identity=33.4101382488479, Blast_Score=167, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6321254, Length=445, Percent_Identity=26.7415730337079, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6321170, Length=316, Percent_Identity=24.6835443037975, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI19922554, Length=427, Percent_Identity=32.3185011709602, Blast_Score=192, Evalue=4e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46045; Mature: 45914

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATDSTHPETLVLHAGYRSDEATNAVAVPIYQTTSYQFRDTEQAANLFALKELGNIYTRI
CCCCCCCCCEEEEECCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHH
MNPTCDALEQRVAALEGGVAALAVSSGQTASTFAVLNLCQAGDNFVTSTDLYGGTWNLFA
CCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEECCCCCCCHHHHH
NTFKQMGIEARFVDPSDPEAFRRATDEKTRCYYAETLPNPKLHVFPIAEVAAIGRALGVP
HHHHHCCCEEEECCCCCHHHHHHCCCCCCEEEEECCCCCCCEEEEEHHHHHHHHHHHCCC
LIVDNTAAPVLCRPLALGAAVVVYSATKYIGGHGTSIGGIVVDGGNFDWEAHGARFPTLT
EEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCC
NPDPSYHGAVWTEAVKPLGPIAYILRMRVILLRDIGAALSPFNAFLLIQGLETLPLRMRA
CCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHEECCCHHCCHHHHH
HCENALKVVDFLKSHPQVTTVIHPSVQTGELARRRDGALAHGQGALVGFELKGGLEAGRA
HHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHCCCCCCCCCCCEEEEEECCCHHHHHH
FIDALKMLYHVANIGDARSLAIHPASTTHSQLSAEDQLATGVSQGYVRLSIGIEHADDII
HHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHCCHHHHHHHHCCCEEEEEEECCHHHHHH
GDLKQALESL
HHHHHHHHCC
>Mature Secondary Structure 
ATDSTHPETLVLHAGYRSDEATNAVAVPIYQTTSYQFRDTEQAANLFALKELGNIYTRI
CCCCCCCCEEEEECCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHH
MNPTCDALEQRVAALEGGVAALAVSSGQTASTFAVLNLCQAGDNFVTSTDLYGGTWNLFA
CCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEECCCCCCCHHHHH
NTFKQMGIEARFVDPSDPEAFRRATDEKTRCYYAETLPNPKLHVFPIAEVAAIGRALGVP
HHHHHCCCEEEECCCCCHHHHHHCCCCCCEEEEECCCCCCCEEEEEHHHHHHHHHHHCCC
LIVDNTAAPVLCRPLALGAAVVVYSATKYIGGHGTSIGGIVVDGGNFDWEAHGARFPTLT
EEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCC
NPDPSYHGAVWTEAVKPLGPIAYILRMRVILLRDIGAALSPFNAFLLIQGLETLPLRMRA
CCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHEECCCHHCCHHHHH
HCENALKVVDFLKSHPQVTTVIHPSVQTGELARRRDGALAHGQGALVGFELKGGLEAGRA
HHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHCCCCCCCCCCCEEEEEECCCHHHHHH
FIDALKMLYHVANIGDARSLAIHPASTTHSQLSAEDQLATGVSQGYVRLSIGIEHADDII
HHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHCCHHHHHHHHCCCEEEEEEECCHHHHHH
GDLKQALESL
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]