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The map label for this gene is yiaE [C]

Identifier: 83593150

GI number: 83593150

Start: 2110944

End: 2111915

Strand: Direct

Name: yiaE [C]

Synonym: Rru_A1815

Alternate gene names: 83593150

Gene position: 2110944-2111915 (Clockwise)

Preceding gene: 83593148

Following gene: 83593151

Centisome position: 48.5

GC content: 67.08

Gene sequence:

>972_bases
GTGCTTGGAAAACCTGTTGTTCTTGTCACCCGCACCCTCCCCGCGGCCGTCGAGGAGCGGCTGCTTGGGGATTACGATGT
TTGGCTGAACCGCGACGACCGGCCAATCCCCCCCGAAGACCTGCCCGCTCTGGCCCGGCGCCTTGGCGCCCAGGCGATGC
TGGTGACGCCCACGGACAGGCTTGAGCGAGCCGTTATCGAGGCCTTGCCTAACAGCGTCGCCATCATCGCCAGCTTCTCG
GTGGGCTACGAGCATATCGACCACAATGCCGCCGCCCGCCGGGGCATTCTGGTGACCAATACGCCGGGCGTCCTCAGCGA
CGCCACCGCCGATATCGCCTTGTTGCTGATGCTGGGCGCGGCGCGGCGCGCCTCGGAAGGCGAGCGGCTGGTGCGCAGCG
GCTATTGGAAAGGACTAACCCCGGTGCAATTGCTCGGGCGCCACCTGCACGGCCAGCGCCTGGGCATCCTGGGCATGGGG
CGGATCGGTCAGGCCCTGGCCGAGCGCGCCCGGCCGCTTGGCCTGGAGATCCATTACCACAACCGCACGCCGATTGCCGA
AGACGCGGCCAAGGGGGCGATCTTCCATGCCACGGTCGAGGATTTGCTGGCGGTCAGCGACGTGCTCAGCCTGCACTGCC
CGGCAACACCCCTGACCCGCAAGCTGCTCAACGCCGAGCGCCTCGCCCTGCTGCCGCCGGGCGCCATCGTCGTCAACACA
GCGCGCGGTATCCTGATCGATGACGAGGCCCTGATCGCCGCCCTGAACAGCGGTCAGGTTTTCGCCGCCGGGCTCGATGT
TTACGACAACGAACCCGACCTTCATCCGGCCTACCGCAGCCTGCCCAATGTTTTCCTGCTGCCCCACCTGGGCAGCGCGA
CGATCGAAACCCGAACCGCCATGGGCTTCTTGGCCCTGGATAATCTTGACGCCTATTTCGGCGGCCGCGAGCCGCCCCAC
CGGATCGCCTGA

Upstream 100 bases:

>100_bases
GGCGCTTTTGAAGACCCGACTCCGCGCGGCGCCCCGCTGGACCTGACCCCGACGACCGATCTTCCCGGGGCCGCCCTGGA
CGAGATATAGGAATGCCTGC

Downstream 100 bases:

>100_bases
CCGCCCTTGGTCAGGCCGACCGTCCGAACGGGAGGGAAAGACCATGACCGACACGCGCGCCGAAGCCGACTTAACCGAGG
TTTGGCGGGCCTGGGCGGCC

Product: glycolate reductase

Products: NA

Alternate protein names: GDH; Glyoxylate reductase; Hydroxypyruvate dehydrogenase; NADH-dependent hydroxypyruvate reductase; HPR [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MLGKPVVLVTRTLPAAVEERLLGDYDVWLNRDDRPIPPEDLPALARRLGAQAMLVTPTDRLERAVIEALPNSVAIIASFS
VGYEHIDHNAAARRGILVTNTPGVLSDATADIALLLMLGAARRASEGERLVRSGYWKGLTPVQLLGRHLHGQRLGILGMG
RIGQALAERARPLGLEIHYHNRTPIAEDAAKGAIFHATVEDLLAVSDVLSLHCPATPLTRKLLNAERLALLPPGAIVVNT
ARGILIDDEALIAALNSGQVFAAGLDVYDNEPDLHPAYRSLPNVFLLPHLGSATIETRTAMGFLALDNLDAYFGGREPPH
RIA

Sequences:

>Translated_323_residues
MLGKPVVLVTRTLPAAVEERLLGDYDVWLNRDDRPIPPEDLPALARRLGAQAMLVTPTDRLERAVIEALPNSVAIIASFS
VGYEHIDHNAAARRGILVTNTPGVLSDATADIALLLMLGAARRASEGERLVRSGYWKGLTPVQLLGRHLHGQRLGILGMG
RIGQALAERARPLGLEIHYHNRTPIAEDAAKGAIFHATVEDLLAVSDVLSLHCPATPLTRKLLNAERLALLPPGAIVVNT
ARGILIDDEALIAALNSGQVFAAGLDVYDNEPDLHPAYRSLPNVFLLPHLGSATIETRTAMGFLALDNLDAYFGGREPPH
RIA
>Mature_323_residues
MLGKPVVLVTRTLPAAVEERLLGDYDVWLNRDDRPIPPEDLPALARRLGAQAMLVTPTDRLERAVIEALPNSVAIIASFS
VGYEHIDHNAAARRGILVTNTPGVLSDATADIALLLMLGAARRASEGERLVRSGYWKGLTPVQLLGRHLHGQRLGILGMG
RIGQALAERARPLGLEIHYHNRTPIAEDAAKGAIFHATVEDLLAVSDVLSLHCPATPLTRKLLNAERLALLPPGAIVVNT
ARGILIDDEALIAALNSGQVFAAGLDVYDNEPDLHPAYRSLPNVFLLPHLGSATIETRTAMGFLALDNLDAYFGGREPPH
RIA

Specific function: Active on hydroxypyruvate and glyoxylate [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=321, Percent_Identity=35.8255451713396, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI23308577, Length=253, Percent_Identity=31.6205533596838, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI61743967, Length=258, Percent_Identity=32.5581395348837, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI4557497, Length=258, Percent_Identity=32.5581395348837, Blast_Score=114, Evalue=8e-26,
Organism=Homo sapiens, GI145580578, Length=239, Percent_Identity=31.7991631799163, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI4557499, Length=239, Percent_Identity=31.7991631799163, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI145580575, Length=239, Percent_Identity=31.7991631799163, Blast_Score=108, Evalue=9e-24,
Organism=Escherichia coli, GI87082289, Length=317, Percent_Identity=35.6466876971609, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1789279, Length=260, Percent_Identity=32.3076923076923, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1787645, Length=291, Percent_Identity=29.8969072164948, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI87081824, Length=192, Percent_Identity=27.6041666666667, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1788660, Length=246, Percent_Identity=25.2032520325203, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17532191, Length=267, Percent_Identity=28.0898876404494, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI25147481, Length=289, Percent_Identity=29.0657439446367, Blast_Score=102, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6324055, Length=274, Percent_Identity=35.7664233576642, Blast_Score=173, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6320925, Length=292, Percent_Identity=30.4794520547945, Blast_Score=129, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6322116, Length=308, Percent_Identity=28.5714285714286, Blast_Score=127, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6321253, Length=218, Percent_Identity=31.1926605504587, Blast_Score=84, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324964, Length=311, Percent_Identity=28.6173633440514, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6325144, Length=304, Percent_Identity=24.3421052631579, Blast_Score=83, Evalue=7e-17,
Organism=Drosophila melanogaster, GI28571528, Length=283, Percent_Identity=38.1625441696113, Blast_Score=176, Evalue=3e-44,
Organism=Drosophila melanogaster, GI24585516, Length=278, Percent_Identity=31.6546762589928, Blast_Score=137, Evalue=7e-33,
Organism=Drosophila melanogaster, GI28574286, Length=281, Percent_Identity=33.4519572953737, Blast_Score=134, Evalue=6e-32,
Organism=Drosophila melanogaster, GI45552429, Length=240, Percent_Identity=35, Blast_Score=131, Evalue=6e-31,
Organism=Drosophila melanogaster, GI24585514, Length=240, Percent_Identity=35, Blast_Score=131, Evalue=6e-31,
Organism=Drosophila melanogaster, GI28574282, Length=240, Percent_Identity=35, Blast_Score=131, Evalue=6e-31,
Organism=Drosophila melanogaster, GI45551003, Length=240, Percent_Identity=35, Blast_Score=130, Evalue=8e-31,
Organism=Drosophila melanogaster, GI28574284, Length=240, Percent_Identity=35, Blast_Score=130, Evalue=9e-31,
Organism=Drosophila melanogaster, GI19921140, Length=287, Percent_Identity=29.9651567944251, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24646446, Length=223, Percent_Identity=37.219730941704, Blast_Score=128, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24646448, Length=223, Percent_Identity=37.219730941704, Blast_Score=128, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24646452, Length=223, Percent_Identity=37.219730941704, Blast_Score=128, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24646450, Length=223, Percent_Identity=37.219730941704, Blast_Score=128, Evalue=6e-30,
Organism=Drosophila melanogaster, GI62472511, Length=224, Percent_Identity=37.9464285714286, Blast_Score=127, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.29 [H]

Molecular weight: Translated: 34791; Mature: 34791

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGKPVVLVTRTLPAAVEERLLGDYDVWLNRDDRPIPPEDLPALARRLGAQAMLVTPTDR
CCCCCEEEEECCCHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHCCCEEEECCHHH
LERAVIEALPNSVAIIASFSVGYEHIDHNAAARRGILVTNTPGVLSDATADIALLLMLGA
HHHHHHHHCCCCEEEEEEECCCHHHHCCCHHHHCCEEEECCCCCCCCHHHHHHHHHHHHH
ARRASEGERLVRSGYWKGLTPVQLLGRHLHGQRLGILGMGRIGQALAERARPLGLEIHYH
HHCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCEECEEECCHHHHHHHHHCCCCEEEEEEC
NRTPIAEDAAKGAIFHATVEDLLAVSDVLSLHCPATPLTRKLLNAERLALLPPGAIVVNT
CCCCCHHHHHCCCEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEECCCCEEEEEC
ARGILIDDEALIAALNSGQVFAAGLDVYDNEPDLHPAYRSLPNVFLLPHLGSATIETRTA
CCCEEEECHHHEEEECCCCEEEEECCCCCCCCCCCHHHHCCCCEEEECCCCCCEEHHHHH
MGFLALDNLDAYFGGREPPHRIA
HEEEEECCCHHHCCCCCCCCCCC
>Mature Secondary Structure
MLGKPVVLVTRTLPAAVEERLLGDYDVWLNRDDRPIPPEDLPALARRLGAQAMLVTPTDR
CCCCCEEEEECCCHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHCCCEEEECCHHH
LERAVIEALPNSVAIIASFSVGYEHIDHNAAARRGILVTNTPGVLSDATADIALLLMLGA
HHHHHHHHCCCCEEEEEEECCCHHHHCCCHHHHCCEEEECCCCCCCCHHHHHHHHHHHHH
ARRASEGERLVRSGYWKGLTPVQLLGRHLHGQRLGILGMGRIGQALAERARPLGLEIHYH
HHCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCEECEEECCHHHHHHHHHCCCCEEEEEEC
NRTPIAEDAAKGAIFHATVEDLLAVSDVLSLHCPATPLTRKLLNAERLALLPPGAIVVNT
CCCCCHHHHHCCCEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEECCCCEEEEEC
ARGILIDDEALIAALNSGQVFAAGLDVYDNEPDLHPAYRSLPNVFLLPHLGSATIETRTA
CCCEEEECHHHEEEECCCCEEEEECCCCCCCCCCCHHHHCCCCEEEECCCCCCEEHHHHH
MGFLALDNLDAYFGGREPPHRIA
HEEEEECCCHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8120891; 2114287 [H]