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The map label for this gene is trmFO

Identifier: 83593092

GI number: 83593092

Start: 2050135

End: 2051583

Strand: Direct

Name: trmFO

Synonym: Rru_A1757

Alternate gene names: 83593092

Gene position: 2050135-2051583 (Clockwise)

Preceding gene: 83593090

Following gene: 83593095

Centisome position: 47.1

GC content: 68.25

Gene sequence:

>1449_bases
ATGACACAGCACCCTCCCCTCACCATCGTCGGCGGCGGTCTTGCCGGATGCGAAGCCGCTTGGCAGGCCGCCCGTGCCGG
GCTGCGCGTGATCCTGATCGAGATGCGGCCGCTCCGCACTACCGAGGCCCATCTCGGCGACGGACTGGCCGAACTGGTCT
GTTCGAATTCGCTGCGCTCGGATGATCCCCTTTATAACGCCGTCGGCCTCCTCCACGAGGAGATGCGCCGCGCCGGATCG
TTGATCCTGGCGATGGCCGAGGCCCACCGGGTTCCCGCCGGCGGCGCCTTGGCTGTTGATCGCCAGGGCTTTTCCGACGC
CATCACCCGCGCCCTGGCCGAGCATCCGCTGATCGAGATCCGCCGCGGCGAGGTCGACCGTCTGCCCGCAGTCGAGGACG
GACCGGCGATCATCGCCAGCGGGCCGCTGACCTCGGCGGCTTTGGCCGCCGCCATCGCCGAGGCGACCGGCGAGACCAGT
CTGGCCTTTTTCGACGCCATCGCCCCGATCGTCCATAAGGACAGCATCGATTTCGACCGCGCCTGGTTCCAGTCGCGCTA
CGACAAGGGCGACGGCCGCGACTACATCAATTGCCCGCTGACCCGCGACCAATACGACGCCTTCGTCGACGCCCTGCTGG
CCGGCGAGAAAACCATGTTCAAGGAATGGGAAGGCACGCCCTATTTCGACGGCTGCCTGCCCATCGAGGTGATGGCCGAG
CGCGGCCGCGAAACCCTGGCCTTCGGACCGATGAAGCCGGTGGGTCTGACCGACCCGCGCAATCCCGGTCTGCGCCCCCA
TGCGGTGGTGCAGCTGCGCCAGGATAACGCCCTGGGAACCCTCTACAACATGGTCGGCTTCCAAACCAAGCTGAAGCATG
GCGAACAGGCGCGGATCTTCCGCATGATCCCCGGGCTGGAAAACGCCGAATTCGCCCGACTCGGCGGCATTCACCGCAAT
ACCTTTCTCAACAGCCCGCGCCTTCTCGATCCGACGCTGCGCCTCAAGGCCCGCCCCCATCTGCGCTTCGCCGGCCAGAT
CACCGGCTGCGAGGGCTATGTGGAAAGCGCCGCCATCGGGCTGCTCGCCGGTCGTTTCGCCAGCGCCGAGATCCTCGACG
CCGCCTCGTTTGCGCCGCCGCCGCCGACAACCGCCCTGGGCGGGCTGCTTGGCCATATCACCGGCGGCGCCAATGCCGAA
ACCTTCCAGCCGATGAATGTCAATTTCGGCCTGTTGCCGCCGCTTGAAGCCCGCCCCGTGCGCCCCGGCGCCAAGCCCCG
GGTGCCCAAGGGCCGCGAGCGCAAGCTGGAATCCGCCCGCCGGGCTTTGGTCGATCTCGGCGATTGGCTGCGCCAGCCCT
CGCCCTGGTCGGCCGAGGATTCCCCGCGCGCCGCCCTTCCCATCCCCGAGCCCACCCCGCTGGGCCCAGCGAGCGGCTCA
AGCGAGTAA

Upstream 100 bases:

>100_bases
TTGTGTTGCCCAGGCGGGGCTTCCTGTCAAGGCCCGCCGCCAAGGGAACAGGCGCGGCTATTTCTAGACAGCCGGCTCGC
GCCGCATTATGGGAGAAGCC

Downstream 100 bases:

>100_bases
AGCAAAGTCCAACAAGGGGTTATGCGTCATTCCTCACAAAAAAACAGGCCCGGAAACGGGCCTGTTGAGAGGGATAGGGA
TGGGGAGGACGGACCAGCAA

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase

Number of amino acids: Translated: 482; Mature: 481

Protein sequence:

>482_residues
MTQHPPLTIVGGGLAGCEAAWQAARAGLRVILIEMRPLRTTEAHLGDGLAELVCSNSLRSDDPLYNAVGLLHEEMRRAGS
LILAMAEAHRVPAGGALAVDRQGFSDAITRALAEHPLIEIRRGEVDRLPAVEDGPAIIASGPLTSAALAAAIAEATGETS
LAFFDAIAPIVHKDSIDFDRAWFQSRYDKGDGRDYINCPLTRDQYDAFVDALLAGEKTMFKEWEGTPYFDGCLPIEVMAE
RGRETLAFGPMKPVGLTDPRNPGLRPHAVVQLRQDNALGTLYNMVGFQTKLKHGEQARIFRMIPGLENAEFARLGGIHRN
TFLNSPRLLDPTLRLKARPHLRFAGQITGCEGYVESAAIGLLAGRFASAEILDAASFAPPPPTTALGGLLGHITGGANAE
TFQPMNVNFGLLPPLEARPVRPGAKPRVPKGRERKLESARRALVDLGDWLRQPSPWSAEDSPRAALPIPEPTPLGPASGS
SE

Sequences:

>Translated_482_residues
MTQHPPLTIVGGGLAGCEAAWQAARAGLRVILIEMRPLRTTEAHLGDGLAELVCSNSLRSDDPLYNAVGLLHEEMRRAGS
LILAMAEAHRVPAGGALAVDRQGFSDAITRALAEHPLIEIRRGEVDRLPAVEDGPAIIASGPLTSAALAAAIAEATGETS
LAFFDAIAPIVHKDSIDFDRAWFQSRYDKGDGRDYINCPLTRDQYDAFVDALLAGEKTMFKEWEGTPYFDGCLPIEVMAE
RGRETLAFGPMKPVGLTDPRNPGLRPHAVVQLRQDNALGTLYNMVGFQTKLKHGEQARIFRMIPGLENAEFARLGGIHRN
TFLNSPRLLDPTLRLKARPHLRFAGQITGCEGYVESAAIGLLAGRFASAEILDAASFAPPPPTTALGGLLGHITGGANAE
TFQPMNVNFGLLPPLEARPVRPGAKPRVPKGRERKLESARRALVDLGDWLRQPSPWSAEDSPRAALPIPEPTPLGPASGS
SE
>Mature_481_residues
TQHPPLTIVGGGLAGCEAAWQAARAGLRVILIEMRPLRTTEAHLGDGLAELVCSNSLRSDDPLYNAVGLLHEEMRRAGSL
ILAMAEAHRVPAGGALAVDRQGFSDAITRALAEHPLIEIRRGEVDRLPAVEDGPAIIASGPLTSAALAAAIAEATGETSL
AFFDAIAPIVHKDSIDFDRAWFQSRYDKGDGRDYINCPLTRDQYDAFVDALLAGEKTMFKEWEGTPYFDGCLPIEVMAER
GRETLAFGPMKPVGLTDPRNPGLRPHAVVQLRQDNALGTLYNMVGFQTKLKHGEQARIFRMIPGLENAEFARLGGIHRNT
FLNSPRLLDPTLRLKARPHLRFAGQITGCEGYVESAAIGLLAGRFASAEILDAASFAPPPPTTALGGLLGHITGGANAET
FQPMNVNFGLLPPLEARPVRPGAKPRVPKGRERKLESARRALVDLGDWLRQPSPWSAEDSPRAALPIPEPTPLGPASGSS
E

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily

Homologues:

Organism=Escherichia coli, GI2367273, Length=118, Percent_Identity=38.135593220339, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMFO_RHORT (Q2RTI8)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_426844.1
- ProteinModelPortal:   Q2RTI8
- SMR:   Q2RTI8
- STRING:   Q2RTI8
- GeneID:   3835179
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A1757
- NMPDR:   fig|1085.1.peg.2332
- eggNOG:   COG1206
- HOGENOM:   HBG285041
- OMA:   FEGCLPI
- ProtClustDB:   PRK05335
- BioCyc:   RRUB269796:RRU_A1757-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01037
- InterPro:   IPR004417
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00137

Pfam domain/function: PF01134 GIDA

EC number: =2.1.1.74

Molecular weight: Translated: 51826; Mature: 51695

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQHPPLTIVGGGLAGCEAAWQAARAGLRVILIEMRPLRTTEAHLGDGLAELVCSNSLRS
CCCCCCEEEECCCHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHCCHHHHHHHCCCCCC
DDPLYNAVGLLHEEMRRAGSLILAMAEAHRVPAGGALAVDRQGFSDAITRALAEHPLIEI
CCHHHHHHHHHHHHHHHHHHEEEEHHHHHCCCCCCEEEECCCCHHHHHHHHHHHCCCEEE
RRGEVDRLPAVEDGPAIIASGPLTSAALAAAIAEATGETSLAFFDAIAPIVHKDSIDFDR
ECCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHH
AWFQSRYDKGDGRDYINCPLTRDQYDAFVDALLAGEKTMFKEWEGTPYFDGCLPIEVMAE
HHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCHHHHHHH
RGRETLAFGPMKPVGLTDPRNPGLRPHAVVQLRQDNALGTLYNMVGFQTKLKHGEQARIF
CCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCHHHHH
RMIPGLENAEFARLGGIHRNTFLNSPRLLDPTLRLKARPHLRFAGQITGCEGYVESAAIG
HHCCCCCCCCHHHHCCCHHHHHCCCCCCCCCHHHCCCCCCEEEECEECCCHHHHHHHHHH
LLAGRFASAEILDAASFAPPPPTTALGGLLGHITGGANAETFQPMNVNFGLLPPLEARPV
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
RPGAKPRVPKGRERKLESARRALVDLGDWLRQPSPWSAEDSPRAALPIPEPTPLGPASGS
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SE
CC
>Mature Secondary Structure 
TQHPPLTIVGGGLAGCEAAWQAARAGLRVILIEMRPLRTTEAHLGDGLAELVCSNSLRS
CCCCCEEEECCCHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHCCHHHHHHHCCCCCC
DDPLYNAVGLLHEEMRRAGSLILAMAEAHRVPAGGALAVDRQGFSDAITRALAEHPLIEI
CCHHHHHHHHHHHHHHHHHHEEEEHHHHHCCCCCCEEEECCCCHHHHHHHHHHHCCCEEE
RRGEVDRLPAVEDGPAIIASGPLTSAALAAAIAEATGETSLAFFDAIAPIVHKDSIDFDR
ECCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHH
AWFQSRYDKGDGRDYINCPLTRDQYDAFVDALLAGEKTMFKEWEGTPYFDGCLPIEVMAE
HHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCHHHHHHH
RGRETLAFGPMKPVGLTDPRNPGLRPHAVVQLRQDNALGTLYNMVGFQTKLKHGEQARIF
CCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCHHHHH
RMIPGLENAEFARLGGIHRNTFLNSPRLLDPTLRLKARPHLRFAGQITGCEGYVESAAIG
HHCCCCCCCCHHHHCCCHHHHHCCCCCCCCCHHHCCCCCCEEEECEECCCHHHHHHHHHH
LLAGRFASAEILDAASFAPPPPTTALGGLLGHITGGANAETFQPMNVNFGLLPPLEARPV
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
RPGAKPRVPKGRERKLESARRALVDLGDWLRQPSPWSAEDSPRAALPIPEPTPLGPASGS
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SE
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA