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The map label for this gene is speE [H]

Identifier: 83593026

GI number: 83593026

Start: 1982256

End: 1983107

Strand: Reverse

Name: speE [H]

Synonym: Rru_A1691

Alternate gene names: 83593026

Gene position: 1983107-1982256 (Counterclockwise)

Preceding gene: 83593030

Following gene: 83593025

Centisome position: 45.56

GC content: 67.72

Gene sequence:

>852_bases
ATGACCGAATGGTTCAGCGAGACCCTTCACCCCCATTGGCGCCAGAGCTTCGCCGCCGACCGCGTGCTCTTGCGCGAACA
GACCGCCCATCAGGATCTGGTGATCCTCGAGAATCAGACCTTCGGCCGGGTGATGGTGCTTGACGGCATGGTGCAGCTGA
CCGAGCGCGACGAATTCGTCTATCACGAGATGATCGCCCATGTGCCGTTGTTCGGCCACGGCGCGGCGCGGCGGGTGCTA
ATCATCGGCGGCGGCGATGGCGGCACGCTGCGCGAAGTCCTGCGCCATAAAACCGTCGAGCGGGCGACCATGGTCGAAAT
CGACGCCACCGTCGTCGAGATGGCGCGCGACCATATGCCGATGGTCAGCGATGGCGCCTTCGACGATCCGCGCGCCGAGC
TGATCATCGCCGACGGCATCGCCTTCGTCGCCGGCGCCCATCAGCCCTATGACGTGATCATCATCGATTCCACCGATCCC
TTCGGTCCGGCCGAGGGGCTGTTCACCCCGGCCTTCTATGGCGCCTGCCGCCAGTTGCTGACCCCGGGCGGCGTGCTGGT
GGTGCAGGCCGGCGTGCCCTTCCTGCAGCCGCGCGAGATCGCCCATGTCAACGCCGCCCTGTCGGCGGCCTTCCCCGAGG
TGGCCTTCTATGCCATCGCCGTTCCCACCTATGTCGGCGGCTTGATGACCCTGGGCTGGGCGAGCGAGGATGCCGGCAAG
AAGGTTCCCGATCCGGCGGTTCTGGCCGCGCGGGTGGCGGCCAGCGGCCTTGCGTTCGGCTATTACTCGCCCGAGGGCCA
TGCCGGCGCCTTCGCCCTGCCCCCCCATATCCGCGCCGCCGCCCGAGGCTGA

Upstream 100 bases:

>100_bases
CGCCGCTCTTTTTCGGACGCAACCGTTGCGCAAGGACCACAAGGTGGGTTTCTCTAGGGCGGTTCTTCAAGTCCCTTCCG
TTGCCAAAGGACGTTTTTTC

Downstream 100 bases:

>100_bases
GCGCGCCCGCTGAAAAAACCGCCGCCGCTCTACAAGGAGATCTCGCCCGTGACCTCGCCTTCGCCGCCGCCCCCGTCGCC
GATCGATCGCTTGCTCGCCC

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY [H]

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MTEWFSETLHPHWRQSFAADRVLLREQTAHQDLVILENQTFGRVMVLDGMVQLTERDEFVYHEMIAHVPLFGHGAARRVL
IIGGGDGGTLREVLRHKTVERATMVEIDATVVEMARDHMPMVSDGAFDDPRAELIIADGIAFVAGAHQPYDVIIIDSTDP
FGPAEGLFTPAFYGACRQLLTPGGVLVVQAGVPFLQPREIAHVNAALSAAFPEVAFYAIAVPTYVGGLMTLGWASEDAGK
KVPDPAVLAARVAASGLAFGYYSPEGHAGAFALPPHIRAAARG

Sequences:

>Translated_283_residues
MTEWFSETLHPHWRQSFAADRVLLREQTAHQDLVILENQTFGRVMVLDGMVQLTERDEFVYHEMIAHVPLFGHGAARRVL
IIGGGDGGTLREVLRHKTVERATMVEIDATVVEMARDHMPMVSDGAFDDPRAELIIADGIAFVAGAHQPYDVIIIDSTDP
FGPAEGLFTPAFYGACRQLLTPGGVLVVQAGVPFLQPREIAHVNAALSAAFPEVAFYAIAVPTYVGGLMTLGWASEDAGK
KVPDPAVLAARVAASGLAFGYYSPEGHAGAFALPPHIRAAARG
>Mature_282_residues
TEWFSETLHPHWRQSFAADRVLLREQTAHQDLVILENQTFGRVMVLDGMVQLTERDEFVYHEMIAHVPLFGHGAARRVLI
IGGGDGGTLREVLRHKTVERATMVEIDATVVEMARDHMPMVSDGAFDDPRAELIIADGIAFVAGAHQPYDVIIIDSTDPF
GPAEGLFTPAFYGACRQLLTPGGVLVVQAGVPFLQPREIAHVNAALSAAFPEVAFYAIAVPTYVGGLMTLGWASEDAGKK
VPDPAVLAARVAASGLAFGYYSPEGHAGAFALPPHIRAAARG

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=279, Percent_Identity=36.2007168458781, Blast_Score=199, Evalue=3e-51,
Organism=Escherichia coli, GI1786312, Length=278, Percent_Identity=53.9568345323741, Blast_Score=312, Evalue=1e-86,
Organism=Caenorhabditis elegans, GI25154106, Length=265, Percent_Identity=41.8867924528302, Blast_Score=197, Evalue=5e-51,
Organism=Caenorhabditis elegans, GI212645616, Length=223, Percent_Identity=46.1883408071749, Blast_Score=185, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6323175, Length=280, Percent_Identity=39.6428571428571, Blast_Score=217, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6325326, Length=274, Percent_Identity=35.7664233576642, Blast_Score=194, Evalue=1e-50,
Organism=Drosophila melanogaster, GI24645443, Length=274, Percent_Identity=37.2262773722628, Blast_Score=191, Evalue=5e-49,
Organism=Drosophila melanogaster, GI21355941, Length=211, Percent_Identity=35.0710900473934, Blast_Score=135, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 30492; Mature: 30361

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1 ; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEWFSETLHPHWRQSFAADRVLLREQTAHQDLVILENQTFGRVMVLDGMVQLTERDEFV
CCCHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEECCHHHHHCCHHHH
YHEMIAHVPLFGHGAARRVLIIGGGDGGTLREVLRHKTVERATMVEIDATVVEMARDHMP
HHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHCCC
MVSDGAFDDPRAELIIADGIAFVAGAHQPYDVIIIDSTDPFGPAEGLFTPAFYGACRQLL
CCCCCCCCCCCCEEEEECCEEEEECCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHH
TPGGVLVVQAGVPFLQPREIAHVNAALSAAFPEVAFYAIAVPTYVGGLMTLGWASEDAGK
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
KVPDPAVLAARVAASGLAFGYYSPEGHAGAFALPPHIRAAARG
CCCCHHHHHHHHHHCCEEEEEECCCCCCCCEECCCCCHHCCCC
>Mature Secondary Structure 
TEWFSETLHPHWRQSFAADRVLLREQTAHQDLVILENQTFGRVMVLDGMVQLTERDEFV
CCHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEECCHHHHHCCHHHH
YHEMIAHVPLFGHGAARRVLIIGGGDGGTLREVLRHKTVERATMVEIDATVVEMARDHMP
HHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHCCC
MVSDGAFDDPRAELIIADGIAFVAGAHQPYDVIIIDSTDPFGPAEGLFTPAFYGACRQLL
CCCCCCCCCCCCEEEEECCEEEEECCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHH
TPGGVLVVQAGVPFLQPREIAHVNAALSAAFPEVAFYAIAVPTYVGGLMTLGWASEDAGK
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
KVPDPAVLAARVAASGLAFGYYSPEGHAGAFALPPHIRAAARG
CCCCHHHHHHHHHHCCEEEEEECCCCCCCCEECCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]