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The map label for this gene is ispDF

Identifier: 83593009

GI number: 83593009

Start: 1963245

End: 1964399

Strand: Reverse

Name: ispDF

Synonym: Rru_A1674

Alternate gene names: 83593009

Gene position: 1964399-1963245 (Counterclockwise)

Preceding gene: 83593024

Following gene: 83593007

Centisome position: 45.13

GC content: 70.39

Gene sequence:

>1155_bases
ATGGCGAAAGTCGCGGCGCTGATCGTGGCCGCCGGGCGCGGAAAGCGCTTTGGCGGCGACCTGCCCAAGCAGTATCAAAC
CCTTGGCGGGCGACCGATCCTCCGCCACACCCTGGCCCGTTTCGCCGCCCACCCCGATGTCGCCCTGGTCCGCGCCATCA
TCCATCCCGAGGATCGCGACCTCTATGGCGCCGCCGCCCATGGCCTCGCCACCCTGCTCGACGCGGTCGAGGGCGGGGCC
GAGCGCCAGGATTCGGTGCGCCTCGGGCTGGAAAGCCTGACCGATGTCGCCCCCGATTACGTGCTGATCCACGATGGCGC
CCGGCCGCTGGTCGATGGCGCGCTGATCGACCGGGTGATCGCCGCCCTGGCCGATCACCCCGGCGCCATTCCCGCCCTGG
CCGTGGCCGACACCCTGAAGCGCGGGGCCGGCGGCATGATCGGCCAAACCGTCGATCGCGCCGGTCTGTGGCGGGCGCAG
ACCCCCCAGGGCTTCCGCTACGACGCCATCCTCGCCGCCCACCGGGCCCAGGCCGGCACCATGCTCACCGATGACGCCGC
CGTTCTCGAAGCCGCCGGCGGCGCCGTCGCCCTGGTCGACGGCGCCGAGGACAACGCCAAGATCACGACCTTCGCCGATC
TGGAGCGGGCCGAACGGCTGTTTCGCGGCGAGGGCGAGCAGCGGATCGGCTCGGGCTTCGATGTCCATCGCCTGGGGCCG
GGGGATTTCGTTACCCTCAACGGCATCCGCATTCCCCATAGCCATGGGTTGGTTGGCCATTCCGATGCGGACGCGCCGAT
GCACGCCCTGACCGACGCCCTGCTCGGCTGCCTGAACGCCGGCGACATCGGCCGGCATTTCCCACCCTCGGACATGCGCT
GGAAGGGGGCGGATTCGGCGATTTTCCTGCGCCATGCGGCGGCGCTGATCACCGCCCTGGGCGGGCGCATCCTCAATGTC
GATATCACGATCTTGTGCGAGCAGCCCAAGATCGGCCCGCACCGCGCCCGCATGTCGGCGCGGCTGGCCGAAATCCTCGA
AATCTCGGCGACCCGCGTCGCCGTCAAGGCCACCACCACCGAGGAACTGGGCTTCACCGGCCGTGGCGAAGGCATCGCCG
CCCAGGCCACGGCGCTGATCACCCTGCCCTTCTAA

Upstream 100 bases:

>100_bases
GATGCGCCCGGAAAACAATCGCCCGCCACGTTCGGCCCTGCTAGGGTTGGGGCCATTTTCGCGTTTATTCCCCTCCTTTA
GGTAGTCAAGGAACCCATCC

Downstream 100 bases:

>100_bases
AAACAAACCTCCAGCCGGCCCGCGTCACCCGGGCCGGCTGTTCGCGGTGATCCAGTCGAGAAACCCGCCATCGCCGGCTT
GCACCGGCAAGGCGACGATG

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; MECDP-synthase; MECPS

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MAKVAALIVAAGRGKRFGGDLPKQYQTLGGRPILRHTLARFAAHPDVALVRAIIHPEDRDLYGAAAHGLATLLDAVEGGA
ERQDSVRLGLESLTDVAPDYVLIHDGARPLVDGALIDRVIAALADHPGAIPALAVADTLKRGAGGMIGQTVDRAGLWRAQ
TPQGFRYDAILAAHRAQAGTMLTDDAAVLEAAGGAVALVDGAEDNAKITTFADLERAERLFRGEGEQRIGSGFDVHRLGP
GDFVTLNGIRIPHSHGLVGHSDADAPMHALTDALLGCLNAGDIGRHFPPSDMRWKGADSAIFLRHAAALITALGGRILNV
DITILCEQPKIGPHRARMSARLAEILEISATRVAVKATTTEELGFTGRGEGIAAQATALITLPF

Sequences:

>Translated_384_residues
MAKVAALIVAAGRGKRFGGDLPKQYQTLGGRPILRHTLARFAAHPDVALVRAIIHPEDRDLYGAAAHGLATLLDAVEGGA
ERQDSVRLGLESLTDVAPDYVLIHDGARPLVDGALIDRVIAALADHPGAIPALAVADTLKRGAGGMIGQTVDRAGLWRAQ
TPQGFRYDAILAAHRAQAGTMLTDDAAVLEAAGGAVALVDGAEDNAKITTFADLERAERLFRGEGEQRIGSGFDVHRLGP
GDFVTLNGIRIPHSHGLVGHSDADAPMHALTDALLGCLNAGDIGRHFPPSDMRWKGADSAIFLRHAAALITALGGRILNV
DITILCEQPKIGPHRARMSARLAEILEISATRVAVKATTTEELGFTGRGEGIAAQATALITLPF
>Mature_383_residues
AKVAALIVAAGRGKRFGGDLPKQYQTLGGRPILRHTLARFAAHPDVALVRAIIHPEDRDLYGAAAHGLATLLDAVEGGAE
RQDSVRLGLESLTDVAPDYVLIHDGARPLVDGALIDRVIAALADHPGAIPALAVADTLKRGAGGMIGQTVDRAGLWRAQT
PQGFRYDAILAAHRAQAGTMLTDDAAVLEAAGGAVALVDGAEDNAKITTFADLERAERLFRGEGEQRIGSGFDVHRLGPG
DFVTLNGIRIPHSHGLVGHSDADAPMHALTDALLGCLNAGDIGRHFPPSDMRWKGADSAIFLRHAAALITALGGRILNVD
ITILCEQPKIGPHRARMSARLAEILEISATRVAVKATTTEELGFTGRGEGIAAQATALITLPF

Specific function: Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl- D-erythritol 4-phosphate (MEP) (ispD), and converts 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C- methyl-D-erythri

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the ispF family

Homologues:

Organism=Homo sapiens, GI157412259, Length=245, Percent_Identity=26.9387755102041, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI1789103, Length=154, Percent_Identity=52.5974025974026, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1789104, Length=224, Percent_Identity=39.2857142857143, Blast_Score=117, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPDF_RHORT (Q2RTS1)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_426761.1
- HSSP:   P62617
- ProteinModelPortal:   Q2RTS1
- SMR:   Q2RTS1
- STRING:   Q2RTS1
- GeneID:   3835094
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A1674
- NMPDR:   fig|1085.1.peg.2251
- eggNOG:   COG1211
- HOGENOM:   HBG672839
- OMA:   IVLIHDA
- PhylomeDB:   Q2RTS1
- ProtClustDB:   CLSK943350
- BioCyc:   RRUB269796:RRU_A1674-MONOMER
- HAMAP:   MF_01520
- InterPro:   IPR001228
- InterPro:   IPR018294
- InterPro:   IPR003526
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00453
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF01128 IspD; PF02542 YgbB; SSF69765 YgbB_synth

EC number: =2.7.7.60; =4.6.1.12

Molecular weight: Translated: 40288; Mature: 40156

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS01295 ISPD; PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKVAALIVAAGRGKRFGGDLPKQYQTLGGRPILRHTLARFAAHPDVALVRAIIHPEDRD
CCCCEEEEEECCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCC
LYGAAAHGLATLLDAVEGGAERQDSVRLGLESLTDVAPDYVLIHDGARPLVDGALIDRVI
CHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHH
AALADHPGAIPALAVADTLKRGAGGMIGQTVDRAGLWRAQTPQGFRYDAILAAHRAQAGT
HHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCC
MLTDDAAVLEAAGGAVALVDGAEDNAKITTFADLERAERLFRGEGEQRIGSGFDVHRLGP
EEECCHHHHHCCCCEEEEEECCCCCCEEEEHHHHHHHHHHHCCCCHHHHCCCCCEEEECC
GDFVTLNGIRIPHSHGLVGHSDADAPMHALTDALLGCLNAGDIGRHFPPSDMRWKGADSA
CCEEEECCEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCH
IFLRHAAALITALGGRILNVDITILCEQPKIGPHRARMSARLAEILEISATRVAVKATTT
HHHHHHHHHHHHHCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHEEEEEECCH
EELGFTGRGEGIAAQATALITLPF
HHCCCCCCCCCEEEEEEEEEEECC
>Mature Secondary Structure 
AKVAALIVAAGRGKRFGGDLPKQYQTLGGRPILRHTLARFAAHPDVALVRAIIHPEDRD
CCCEEEEEECCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCC
LYGAAAHGLATLLDAVEGGAERQDSVRLGLESLTDVAPDYVLIHDGARPLVDGALIDRVI
CHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHH
AALADHPGAIPALAVADTLKRGAGGMIGQTVDRAGLWRAQTPQGFRYDAILAAHRAQAGT
HHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCC
MLTDDAAVLEAAGGAVALVDGAEDNAKITTFADLERAERLFRGEGEQRIGSGFDVHRLGP
EEECCHHHHHCCCCEEEEEECCCCCCEEEEHHHHHHHHHHHCCCCHHHHCCCCCEEEECC
GDFVTLNGIRIPHSHGLVGHSDADAPMHALTDALLGCLNAGDIGRHFPPSDMRWKGADSA
CCEEEECCEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCH
IFLRHAAALITALGGRILNVDITILCEQPKIGPHRARMSARLAEILEISATRVAVKATTT
HHHHHHHHHHHHHCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHEEEEEECCH
EELGFTGRGEGIAAQATALITLPF
HHCCCCCCCCCEEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA