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The map label for this gene is nuoB

Identifier: 83592891

GI number: 83592891

Start: 1836556

End: 1837113

Strand: Direct

Name: nuoB

Synonym: Rru_A1556

Alternate gene names: 83592891

Gene position: 1836556-1837113 (Clockwise)

Preceding gene: 83592890

Following gene: 83592892

Centisome position: 42.19

GC content: 65.05

Gene sequence:

>558_bases
ATGGGAGTGAGCACGCCCGTGATCGTTCCGCGGGGCGGGGCGCCGGTGCCGCCGGGCCCCGATCAGGATCTGATCCTTGG
TCAGGTCGCCCGCGAGATCGAGGAAAAGGGCTTCGTTCTGTCCAATGTCGACAGTCTGGTCAACTGGGCGCGCGCCGGAT
CGCTATGGCCGATGACCTTCGGGCTCGCCTGCTGCGCCCTGGAAATGATCCATTGCGCCTGCGCCCGCTATGATCTTGAC
CGCTTCGGCACGGTTTTCCGGCCCAGTCCGCGCCAGTCCGACGTGATGATTGTCGCCGGCACCCTGACGAACAAGATGGC
CCCGGCGCTGCGCAAGGTTTACGACCAGATGGCGGAGCCGCGCTGGGTGATCTCGATGGGCAGTTGCGCCAATGGCGGCG
GCTATTATCACTACTCCTATTCGGTGGTGCGCGGCTGCGACCGCATCGTGCCGGTCGACATCTATGTGCCGGGCTGCCCG
CCGACCTCGGAGGCCCTGCTGTACGGGGTGTTGCAGCTGCAAAAGAAGATCAAGCGGACCGGCGCGCTTTACCGGTGA

Upstream 100 bases:

>100_bases
GCCTGGGCGCGATCGGCGTCTTCGGCTTCTGGTCGATGGTCGTGTTTCTTGGCGTGCTGACCGTGGGCTTCGTCTACGAA
TGGAAGAAGGGAGCTTTGGA

Downstream 100 bases:

>100_bases
CCGGTCGGCAACGCCTTAGAGTGGGGAACGGCGCGGAATGACGAGTGTTTTCGAAGAACGGGTCGAGAGCCTCAAGGAAC
TTGGGGCCCGCATCGAAGCC

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B

Number of amino acids: Translated: 185; Mature: 184

Protein sequence:

>185_residues
MGVSTPVIVPRGGAPVPPGPDQDLILGQVAREIEEKGFVLSNVDSLVNWARAGSLWPMTFGLACCALEMIHCACARYDLD
RFGTVFRPSPRQSDVMIVAGTLTNKMAPALRKVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCP
PTSEALLYGVLQLQKKIKRTGALYR

Sequences:

>Translated_185_residues
MGVSTPVIVPRGGAPVPPGPDQDLILGQVAREIEEKGFVLSNVDSLVNWARAGSLWPMTFGLACCALEMIHCACARYDLD
RFGTVFRPSPRQSDVMIVAGTLTNKMAPALRKVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCP
PTSEALLYGVLQLQKKIKRTGALYR
>Mature_184_residues
GVSTPVIVPRGGAPVPPGPDQDLILGQVAREIEEKGFVLSNVDSLVNWARAGSLWPMTFGLACCALEMIHCACARYDLDR
FGTVFRPSPRQSDVMIVAGTLTNKMAPALRKVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPP
TSEALLYGVLQLQKKIKRTGALYR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=146, Percent_Identity=82.1917808219178, Blast_Score=261, Evalue=2e-70,
Organism=Escherichia coli, GI1788624, Length=180, Percent_Identity=45, Blast_Score=171, Evalue=2e-44,
Organism=Escherichia coli, GI1789074, Length=126, Percent_Identity=41.2698412698413, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1788834, Length=123, Percent_Identity=38.2113821138211, Blast_Score=88, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17509685, Length=142, Percent_Identity=73.943661971831, Blast_Score=242, Evalue=1e-64,
Organism=Drosophila melanogaster, GI18859983, Length=176, Percent_Identity=69.3181818181818, Blast_Score=263, Evalue=5e-71,
Organism=Drosophila melanogaster, GI24642371, Length=176, Percent_Identity=69.3181818181818, Blast_Score=263, Evalue=5e-71,
Organism=Drosophila melanogaster, GI24651058, Length=146, Percent_Identity=78.0821917808219, Blast_Score=252, Evalue=8e-68,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB_RHORT (Q2RU39)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_426643.1
- HSSP:   Q56218
- ProteinModelPortal:   Q2RU39
- SMR:   Q2RU39
- STRING:   Q2RU39
- GeneID:   3834973
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A1556
- NMPDR:   fig|1085.1.peg.2811
- eggNOG:   COG0377
- HOGENOM:   HBG553221
- OMA:   GKPIGSN
- PhylomeDB:   Q2RU39
- ProtClustDB:   PRK06411
- BioCyc:   RRUB269796:RRU_A1556-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 20199; Mature: 20068

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
7.6 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVSTPVIVPRGGAPVPPGPDQDLILGQVAREIEEKGFVLSNVDSLVNWARAGSLWPMTF
CCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHH
GLACCALEMIHCACARYDLDRFGTVFRPSPRQSDVMIVAGTLTNKMAPALRKVYDQMAEP
HHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCC
RWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTSEALLYGVLQLQKKIKRT
EEEEEECCCCCCCCEEEEHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
GALYR
CCCCC
>Mature Secondary Structure 
GVSTPVIVPRGGAPVPPGPDQDLILGQVAREIEEKGFVLSNVDSLVNWARAGSLWPMTF
CCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHH
GLACCALEMIHCACARYDLDRFGTVFRPSPRQSDVMIVAGTLTNKMAPALRKVYDQMAEP
HHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCC
RWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTSEALLYGVLQLQKKIKRT
EEEEEECCCCCCCCEEEEHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
GALYR
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA