Click here to switch to the map view.

The map label for this gene is asnB [H]

Identifier: 83592819

GI number: 83592819

Start: 1749861

End: 1751711

Strand: Reverse

Name: asnB [H]

Synonym: Rru_A1483

Alternate gene names: 83592819

Gene position: 1751711-1749861 (Counterclockwise)

Preceding gene: 83592820

Following gene: 83592811

Centisome position: 40.24

GC content: 71.64

Gene sequence:

>1851_bases
ATGTGCGGGCTGGTGGCGATTTTGGAGCGACGGGGACCGGCCGACGAAGCGGCGGTGTCCAAGGCGCTCGCCCGTCTGGC
GCGGCGCGGTCCCGATGGCGAGGGCCGGGCGGCGTTCTGGGACGGCCGTCTGCTGCTCGGTCATCGGCGGCTGGCGATTT
TCGGCAAGGGCGCCGACGGCGCCCAGCCGATGGTCGATCCCGAGACCGGCAGCGTGCTGGTCTTCAATGGCGCGCTCTAT
AACTATGTCGAACTGCGCCAGGCCCTTCAGGCCCTGGGCCAGCGTTTCGCCGGCGAGAGCGACACCGAGGTCGCTCTGCT
CGCCTGGCGGCAGTGGGGCGAGGCGGCCTTCGCCCGCTTCAACGGCATGTGGGCCATGGTCCTCCTCGACGCCCCAGGCG
ATCGGCTGGTGGTGTCGCGCGATCGCCTGGGCGTCAAACCGCTGTACTTGTGCGACACCGGCGGCCGCGTGACCTTCGCC
AGCACCATCGCCGCCACCCTGGCGGCCAGTGGCATCGCCCCCCGGGTCGATGACGACGCGGTCTTCGATTTCCTGGTCGG
CGGTCTGACCGACCATCGCGGCGGCAGCTTCTTCGCCGGCATCGTCGAGGTTCCCGCCGGCGCCCTGTGGTCGGTCGACC
GGAGCGGGGGCATCGACCGCCGCCGCTTCCACGATTGGCGAAAGGAGGGCAGCGGGGAAGCAGCGCCGCCGTCGGCCGAA
GCGCTGCGCGCGACCTTGAGCGATGCCGTGCGCCTGCGCCTGCGCGCCGATGTTCCGGCGGTCGCCCTGCTCTCGGGCGG
GCTTGATTCCTCGATCGTCACCCGCTTGGCCACGGCGCCCGACATGCCGCGCGGCGCCCTGGCCGGGCTGCTGACCTATG
GCTATCGCGACGCCACCGAAGCCGATGAGACGCGCGAGGCCGGCCGCTTCGCCGCCACGCTCGACAGCGCCCTGCCCCAC
GACATCCTGCGCGTCGCCCCGCTGCCCGCCGCTCCGGACCTTGACGCCCTGCTCGCCGCCCAGGAGCAGCCTTTCAACAC
CCCCTCGCTGGTCGCCGCCTTCCGACTTTATCGGTTGATCGCGGCGCGCGGCGTCAAGGTGGCGATCACCGGCGAGGGCG
CCGACGAGTTGTTCGCCGGCTATACGAACCGCTATGGCGCCCTTCTCGCCCGCGAGCACCTGCGCCTGGGCCGCCTCCGC
CCCTTGGGCGCCCTGCTCGGCTCGGCCCATTGCCCGCCGCGGCTGGTGGCCAACCGCCTCGCCTGGGATCTTCCCTTGGC
CATCGTCCGCCCGTTGATGGCCGGGCGCGTCCATGGCCGGGTGATCGCCCGATCGTTCTGGCAAGACTCGGCCGAACGCT
TCGAGGCCCTGGCGGCCTATCACCACCAGCCGCTGGCGTCGCGCCTGCGCGATGACGCCCTGCGCGCCCTGCTGCCCTAT
TCCCTGCGCTTCGCCGATCGCAGCGGCATGGCCTCGGGCGTCGAGGTGCGGTCGCCGTTCCTCGATTACCGGGTGGTCGA
AGCCGCCCTCGCCCTGCCGGTCGCCGCGAAGATCGACGATCGGGGCGGCAAGCGCATCCTACGCCAAGCCTTCCGCCACG
ACCTACCGGCCGAAATCATCGGCGCGGGCAAAACCCATGGCCTGGGCATGGCCGAACAGTTTCAGGTCGGACACCTTGAT
TGGGCAGCCTTGGCCGAGGCCCCGCCGACCCTGGCGGCGCGCTATCTTGATCTCGGGCGCCTGAAAACCGCCCTGGCCGC
CCACCCCAGTGATCCCAGGCTGTGGTGGCCGCTCTGCCTGCTGCGCTGGCTGGCCTGGGTGGAAGGCGGCATGGCCGACG
CCCCCCGCTAG

Upstream 100 bases:

>100_bases
CTCGGTCCGCGCTATCTGAAAAGCAACAGCCTCTTTCTGGGACTGGTGATCAAGGAATGGATCAGGCTCAGGCGCGGCGG
GGCGGAGGACACCTAGGATC

Downstream 100 bases:

>100_bases
AGTGAATAGAGTAAAACGGTCAATAGGCGTTGGGATCGCGGATCAGGCGGAGCGGCGTCAGGGCGAGGATCCACAGATCA
AGCCCAAGACTCCAGTGGTC

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 616; Mature: 616

Protein sequence:

>616_residues
MCGLVAILERRGPADEAAVSKALARLARRGPDGEGRAAFWDGRLLLGHRRLAIFGKGADGAQPMVDPETGSVLVFNGALY
NYVELRQALQALGQRFAGESDTEVALLAWRQWGEAAFARFNGMWAMVLLDAPGDRLVVSRDRLGVKPLYLCDTGGRVTFA
STIAATLAASGIAPRVDDDAVFDFLVGGLTDHRGGSFFAGIVEVPAGALWSVDRSGGIDRRRFHDWRKEGSGEAAPPSAE
ALRATLSDAVRLRLRADVPAVALLSGGLDSSIVTRLATAPDMPRGALAGLLTYGYRDATEADETREAGRFAATLDSALPH
DILRVAPLPAAPDLDALLAAQEQPFNTPSLVAAFRLYRLIAARGVKVAITGEGADELFAGYTNRYGALLAREHLRLGRLR
PLGALLGSAHCPPRLVANRLAWDLPLAIVRPLMAGRVHGRVIARSFWQDSAERFEALAAYHHQPLASRLRDDALRALLPY
SLRFADRSGMASGVEVRSPFLDYRVVEAALALPVAAKIDDRGGKRILRQAFRHDLPAEIIGAGKTHGLGMAEQFQVGHLD
WAALAEAPPTLAARYLDLGRLKTALAAHPSDPRLWWPLCLLRWLAWVEGGMADAPR

Sequences:

>Translated_616_residues
MCGLVAILERRGPADEAAVSKALARLARRGPDGEGRAAFWDGRLLLGHRRLAIFGKGADGAQPMVDPETGSVLVFNGALY
NYVELRQALQALGQRFAGESDTEVALLAWRQWGEAAFARFNGMWAMVLLDAPGDRLVVSRDRLGVKPLYLCDTGGRVTFA
STIAATLAASGIAPRVDDDAVFDFLVGGLTDHRGGSFFAGIVEVPAGALWSVDRSGGIDRRRFHDWRKEGSGEAAPPSAE
ALRATLSDAVRLRLRADVPAVALLSGGLDSSIVTRLATAPDMPRGALAGLLTYGYRDATEADETREAGRFAATLDSALPH
DILRVAPLPAAPDLDALLAAQEQPFNTPSLVAAFRLYRLIAARGVKVAITGEGADELFAGYTNRYGALLAREHLRLGRLR
PLGALLGSAHCPPRLVANRLAWDLPLAIVRPLMAGRVHGRVIARSFWQDSAERFEALAAYHHQPLASRLRDDALRALLPY
SLRFADRSGMASGVEVRSPFLDYRVVEAALALPVAAKIDDRGGKRILRQAFRHDLPAEIIGAGKTHGLGMAEQFQVGHLD
WAALAEAPPTLAARYLDLGRLKTALAAHPSDPRLWWPLCLLRWLAWVEGGMADAPR
>Mature_616_residues
MCGLVAILERRGPADEAAVSKALARLARRGPDGEGRAAFWDGRLLLGHRRLAIFGKGADGAQPMVDPETGSVLVFNGALY
NYVELRQALQALGQRFAGESDTEVALLAWRQWGEAAFARFNGMWAMVLLDAPGDRLVVSRDRLGVKPLYLCDTGGRVTFA
STIAATLAASGIAPRVDDDAVFDFLVGGLTDHRGGSFFAGIVEVPAGALWSVDRSGGIDRRRFHDWRKEGSGEAAPPSAE
ALRATLSDAVRLRLRADVPAVALLSGGLDSSIVTRLATAPDMPRGALAGLLTYGYRDATEADETREAGRFAATLDSALPH
DILRVAPLPAAPDLDALLAAQEQPFNTPSLVAAFRLYRLIAARGVKVAITGEGADELFAGYTNRYGALLAREHLRLGRLR
PLGALLGSAHCPPRLVANRLAWDLPLAIVRPLMAGRVHGRVIARSFWQDSAERFEALAAYHHQPLASRLRDDALRALLPY
SLRFADRSGMASGVEVRSPFLDYRVVEAALALPVAAKIDDRGGKRILRQAFRHDLPAEIIGAGKTHGLGMAEQFQVGHLD
WAALAEAPPTLAARYLDLGRLKTALAAHPSDPRLWWPLCLLRWLAWVEGGMADAPR

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786889, Length=424, Percent_Identity=29.4811320754717, Blast_Score=126, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6321563, Length=416, Percent_Identity=27.4038461538462, Blast_Score=120, Evalue=8e-28,
Organism=Saccharomyces cerevisiae, GI6325403, Length=414, Percent_Identity=28.0193236714976, Blast_Score=117, Evalue=4e-27,
Organism=Drosophila melanogaster, GI45553209, Length=398, Percent_Identity=29.3969849246231, Blast_Score=115, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 66400; Mature: 66400

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGLVAILERRGPADEAAVSKALARLARRGPDGEGRAAFWDGRLLLGHRRLAIFGKGADG
CCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCEEEECCCEEEEEECCCCC
AQPMVDPETGSVLVFNGALYNYVELRQALQALGQRFAGESDTEVALLAWRQWGEAAFARF
CCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHH
NGMWAMVLLDAPGDRLVVSRDRLGVKPLYLCDTGGRVTFASTIAATLAASGIAPRVDDDA
CCEEEEEEEECCCCEEEEECCCCCCCEEEEECCCCCEEHHHHHHHHHHHCCCCCCCCHHH
VFDFLVGGLTDHRGGSFFAGIVEVPAGALWSVDRSGGIDRRRFHDWRKEGSGEAAPPSAE
HHHHHHHCCCCCCCCCEEEHHHHCCCCCEEECCCCCCCCHHHHHHHHHCCCCCCCCCHHH
ALRATLSDAVRLRLRADVPAVALLSGGLDSSIVTRLATAPDMPRGALAGLLTYGYRDATE
HHHHHHHHHHHHHHHCCCCEEHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCC
ADETREAGRFAATLDSALPHDILRVAPLPAAPDLDALLAAQEQPFNTPSLVAAFRLYRLI
CHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHH
AARGVKVAITGEGADELFAGYTNRYGALLAREHLRLGRLRPLGALLGSAHCPPRLVANRL
HHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHH
AWDLPLAIVRPLMAGRVHGRVIARSFWQDSAERFEALAAYHHQPLASRLRDDALRALLPY
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
SLRFADRSGMASGVEVRSPFLDYRVVEAALALPVAAKIDDRGGKRILRQAFRHDLPAEII
HHEECCCCCCCCCCCHHCCHHHHHHHHHHHHCCHHEECCCCCHHHHHHHHHHCCCCHHHH
GAGKTHGLGMAEQFQVGHLDWAALAEAPPTLAARYLDLGRLKTALAAHPSDPRLWWPLCL
CCCCCCCCCCHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
LRWLAWVEGGMADAPR
HHHHHHHCCCCCCCCC
>Mature Secondary Structure
MCGLVAILERRGPADEAAVSKALARLARRGPDGEGRAAFWDGRLLLGHRRLAIFGKGADG
CCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCEEEECCCEEEEEECCCCC
AQPMVDPETGSVLVFNGALYNYVELRQALQALGQRFAGESDTEVALLAWRQWGEAAFARF
CCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHH
NGMWAMVLLDAPGDRLVVSRDRLGVKPLYLCDTGGRVTFASTIAATLAASGIAPRVDDDA
CCEEEEEEEECCCCEEEEECCCCCCCEEEEECCCCCEEHHHHHHHHHHHCCCCCCCCHHH
VFDFLVGGLTDHRGGSFFAGIVEVPAGALWSVDRSGGIDRRRFHDWRKEGSGEAAPPSAE
HHHHHHHCCCCCCCCCEEEHHHHCCCCCEEECCCCCCCCHHHHHHHHHCCCCCCCCCHHH
ALRATLSDAVRLRLRADVPAVALLSGGLDSSIVTRLATAPDMPRGALAGLLTYGYRDATE
HHHHHHHHHHHHHHHCCCCEEHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCC
ADETREAGRFAATLDSALPHDILRVAPLPAAPDLDALLAAQEQPFNTPSLVAAFRLYRLI
CHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHH
AARGVKVAITGEGADELFAGYTNRYGALLAREHLRLGRLRPLGALLGSAHCPPRLVANRL
HHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHH
AWDLPLAIVRPLMAGRVHGRVIARSFWQDSAERFEALAAYHHQPLASRLRDDALRALLPY
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
SLRFADRSGMASGVEVRSPFLDYRVVEAALALPVAAKIDDRGGKRILRQAFRHDLPAEII
HHEECCCCCCCCCCCHHCCHHHHHHHHHHHHCCHHEECCCCCHHHHHHHHHHCCCCHHHH
GAGKTHGLGMAEQFQVGHLDWAALAEAPPTLAARYLDLGRLKTALAAHPSDPRLWWPLCL
CCCCCCCCCCHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
LRWLAWVEGGMADAPR
HHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]