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The map label for this gene is priA

Identifier: 83592558

GI number: 83592558

Start: 1441961

End: 1444396

Strand: Direct

Name: priA

Synonym: Rru_A1222

Alternate gene names: 83592558

Gene position: 1441961-1444396 (Clockwise)

Preceding gene: 83592555

Following gene: 83592559

Centisome position: 33.13

GC content: 71.14

Gene sequence:

>2436_bases
ATGGCCGGTCTCCCGGCTTTGCCCCCCGGTTCCCGGGAGCTTTTCCCCGAAGACGCCCACGCCGAGCCCGTCGTCGCCGT
GTTGCTGCCGCTGCCTTTGGCCGGGGCCTATGACTATAAGGTGCCCGCCGGCATGGCCCGGCCCGCCGTCGGTACCCTGG
TGCGGGTGCCGCTGGGGCGACGGGAGGAAATCGGCGTGGTTTGGGGGGCGGGGGCGGGCGAAACCCCGCCCGAACGCCTG
AAGCCGCTGATCGGCTTTCCCGAGTGTCCGCCCCTGCCCGCGCCTTTGCGCGCTTTTATCGACTGGGTTGCGGCTTATAC
GGTGCAGCCGCCCGGGGCGGTGTTGCGCATGGCGCTCAGCGTGCCCGCCGCCCTGGAAGCGCCGCCGCCGGCCCTTGGCT
GGCGCCGTCCTTCGGCGGGTCAGCGGGCGGCGGGTCAGCGGGCGGAGGGGCAGGGGCCGCTTCCGGGGGGGGCGCGGCTG
AGCCCCGGCCGCCAAAGGGTTCTGGCGGTTCTTGACGACCATCCCGGTCTGCCCTTCGCCGGCGCCGATCTGGCGCGGGA
AGCGGCCGTGGGGCCGGCGGTGGTGGCGGCGATGGCCAAGGCCGGGCTGCTCGAGGCGGTGACCCGTTCGAATGAATGGT
CGCCGCAAGCCCCCGATGCCGACCGGCCCGGACCGCTGCTGTCGGCCGATCAACAGGCGGCGGCGGACGGCCTGCGCACG
GCGCTTGACCAGGGATTTTCCGGTCTGTTGCTGGAAGGGGTCACCGGCTCGGGCAAGACCGAGGTCTATTTCGAGGCGAT
CGCCGAAACCCTGCGCCGCGGGCGGCAGGCTCTGGTGCTGTTGCCCGAAATCGCCCTGGCCGCCCAATGGCCGCGGCGCT
TCGCCGACCGCTTCGGCGCCGCGCCGGTGCAATGGCATTCGCAAATGGGCGCGGCGGCGCGGCGGCGGGCTTGGCGGGCG
GTGGCCCTGGGGCGGGCGCCGGTGGTGGTGGGGGCGCGATCGGCGCTGTTTCTGCCCTATCCCGATCTGGGCTTGATCAT
CGTCGATGAAGAACACGACAGCGCCTTCAAACAAGAGGAGGGCGTGCCGTATAACGCCCGCGACATGGCCGTGGTCCGCG
CCCGCCTCGGCGGCTTTCCCGCCGTGCTCGCCTCGGCCACGCCGTCGCTGGAAACCATCGAGAACGCCCGCCAGGGCCGC
TATCGGCATCTGGTGCTGCCCCGTCGCCACGGCGGAGCGGAAATGCCCGAGATCACCCTGCTCGATCTGCGCCGCGCCCC
GCCGCAAAAATGGCTGCCCACGGATTTTGCCGGACCCGGCGGGTCCGAGGGTCTGGCCGCGCCCGGCGGGGCGAACGACG
AGGCCGAGGAGCAAAAAGCGCCCCCCCCTTCGCCTACGGCCTCCCCTTCGCCTACGGCCTCTCCTTCGCCCATGGCCCGC
CCGGCCCGCTTGGGCTGGCTGAGCCCGCCGCTGATCACCGCCGTCGAGGAAACCCTGGCGGCGGGCGAGCAGGTTCTGCT
GTTTCTCAATCGCCGGGGCTATGCGCCGCTGACCCTGTGTCGGAGCTGCGGTCATCGGCTGAAATGCCCGCGCTGCACCG
CTTGGCTGGTTGAACACCGCCGCGATGGGCGCCTGCGCTGTCACCATTGCGGCTATCAGCAGCCGATCCCCGAGACCTGT
CCGGCCTGTGGGGTGGCCGATTCCCTGGCGCCTTGCGGCCCGGGCGTCGAGCGATTGGCCGAAGAGGCCGCCCATCGCTT
TCCTAAGGCGCGCATGGATGTTGCCGCCAGCGATACGGTGACCGGACCGAAAGAGGCCGCCGCCCTGGCGACACGAATCG
CCAATCACGACATCGATCTGATCATCGGCACCCAGATCATGGCCAAGGGCCACCACTTTCCGCTGATCACCCTGGTCGGC
GTCGTCGATGGCGATTTGGGGCTAACCGGCGGCGATCTGCGGGCTTCGGAACGAACCCACCAGCTGCTCCATCAGGTCGC
GGGCCGGGCCGGGCGGGCCGAGCGGCCGGGGCGGGTGCTGATCCAGACCGTCGATCCCGGCCATCCGGTGATGGAGGCCC
TGGCCTCGGGCGATCCGGCGCTGTTTCTCGAGGTCGAGGCGGCCGAGCGTCAGGCCCTGGCGATGCCCCCCTTCGGCCGG
CTGGTCGCCCTGGTGATCTCGGGCGAGGACTCTGCCCGGGTCCAGGCCGTGGCCGCGGCCCTTGGGCGGGCGGCGCCGAT
GGGGCCTGGCCTTGATGTGCTGGGGCCCGTACCCGCGCCGCTTGCAATGTTGCGTGGACGCCACCGACACAGGCTGTTGC
TCAAGGCGGCGCGGGGGGTAAAGGTGCAACCGGTGGTGCGCCACTGGCTATCGCTCGTATCGATTCCGCCCGGGGTGAAG
GTTCAGGTGGACGTGGACCCCATTTCCTTTCTATAG

Upstream 100 bases:

>100_bases
CGGAACGGGTGCTTGCTCCCCCCGGGGGTATAGCCCCTGGTCCTCCCCTTTTAAAGCGCCGCATCAGGGTATCGCCCCCC
GATGGCGCGGAGGTTCGCCG

Downstream 100 bases:

>100_bases
TCCGCGCCGTCTTGACATGGACCAAACCTCCCGGGGGACGGGTGGGGGAACGTCGGGGAAAGACACGGTTTTCGGGGCTT
GCCGGCGCGACCAGCGCTTG

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y

Number of amino acids: Translated: 811; Mature: 810

Protein sequence:

>811_residues
MAGLPALPPGSRELFPEDAHAEPVVAVLLPLPLAGAYDYKVPAGMARPAVGTLVRVPLGRREEIGVVWGAGAGETPPERL
KPLIGFPECPPLPAPLRAFIDWVAAYTVQPPGAVLRMALSVPAALEAPPPALGWRRPSAGQRAAGQRAEGQGPLPGGARL
SPGRQRVLAVLDDHPGLPFAGADLAREAAVGPAVVAAMAKAGLLEAVTRSNEWSPQAPDADRPGPLLSADQQAAADGLRT
ALDQGFSGLLLEGVTGSGKTEVYFEAIAETLRRGRQALVLLPEIALAAQWPRRFADRFGAAPVQWHSQMGAAARRRAWRA
VALGRAPVVVGARSALFLPYPDLGLIIVDEEHDSAFKQEEGVPYNARDMAVVRARLGGFPAVLASATPSLETIENARQGR
YRHLVLPRRHGGAEMPEITLLDLRRAPPQKWLPTDFAGPGGSEGLAAPGGANDEAEEQKAPPPSPTASPSPTASPSPMAR
PARLGWLSPPLITAVEETLAAGEQVLLFLNRRGYAPLTLCRSCGHRLKCPRCTAWLVEHRRDGRLRCHHCGYQQPIPETC
PACGVADSLAPCGPGVERLAEEAAHRFPKARMDVAASDTVTGPKEAAALATRIANHDIDLIIGTQIMAKGHHFPLITLVG
VVDGDLGLTGGDLRASERTHQLLHQVAGRAGRAERPGRVLIQTVDPGHPVMEALASGDPALFLEVEAAERQALAMPPFGR
LVALVISGEDSARVQAVAAALGRAAPMGPGLDVLGPVPAPLAMLRGRHRHRLLLKAARGVKVQPVVRHWLSLVSIPPGVK
VQVDVDPISFL

Sequences:

>Translated_811_residues
MAGLPALPPGSRELFPEDAHAEPVVAVLLPLPLAGAYDYKVPAGMARPAVGTLVRVPLGRREEIGVVWGAGAGETPPERL
KPLIGFPECPPLPAPLRAFIDWVAAYTVQPPGAVLRMALSVPAALEAPPPALGWRRPSAGQRAAGQRAEGQGPLPGGARL
SPGRQRVLAVLDDHPGLPFAGADLAREAAVGPAVVAAMAKAGLLEAVTRSNEWSPQAPDADRPGPLLSADQQAAADGLRT
ALDQGFSGLLLEGVTGSGKTEVYFEAIAETLRRGRQALVLLPEIALAAQWPRRFADRFGAAPVQWHSQMGAAARRRAWRA
VALGRAPVVVGARSALFLPYPDLGLIIVDEEHDSAFKQEEGVPYNARDMAVVRARLGGFPAVLASATPSLETIENARQGR
YRHLVLPRRHGGAEMPEITLLDLRRAPPQKWLPTDFAGPGGSEGLAAPGGANDEAEEQKAPPPSPTASPSPTASPSPMAR
PARLGWLSPPLITAVEETLAAGEQVLLFLNRRGYAPLTLCRSCGHRLKCPRCTAWLVEHRRDGRLRCHHCGYQQPIPETC
PACGVADSLAPCGPGVERLAEEAAHRFPKARMDVAASDTVTGPKEAAALATRIANHDIDLIIGTQIMAKGHHFPLITLVG
VVDGDLGLTGGDLRASERTHQLLHQVAGRAGRAERPGRVLIQTVDPGHPVMEALASGDPALFLEVEAAERQALAMPPFGR
LVALVISGEDSARVQAVAAALGRAAPMGPGLDVLGPVPAPLAMLRGRHRHRLLLKAARGVKVQPVVRHWLSLVSIPPGVK
VQVDVDPISFL
>Mature_810_residues
AGLPALPPGSRELFPEDAHAEPVVAVLLPLPLAGAYDYKVPAGMARPAVGTLVRVPLGRREEIGVVWGAGAGETPPERLK
PLIGFPECPPLPAPLRAFIDWVAAYTVQPPGAVLRMALSVPAALEAPPPALGWRRPSAGQRAAGQRAEGQGPLPGGARLS
PGRQRVLAVLDDHPGLPFAGADLAREAAVGPAVVAAMAKAGLLEAVTRSNEWSPQAPDADRPGPLLSADQQAAADGLRTA
LDQGFSGLLLEGVTGSGKTEVYFEAIAETLRRGRQALVLLPEIALAAQWPRRFADRFGAAPVQWHSQMGAAARRRAWRAV
ALGRAPVVVGARSALFLPYPDLGLIIVDEEHDSAFKQEEGVPYNARDMAVVRARLGGFPAVLASATPSLETIENARQGRY
RHLVLPRRHGGAEMPEITLLDLRRAPPQKWLPTDFAGPGGSEGLAAPGGANDEAEEQKAPPPSPTASPSPTASPSPMARP
ARLGWLSPPLITAVEETLAAGEQVLLFLNRRGYAPLTLCRSCGHRLKCPRCTAWLVEHRRDGRLRCHHCGYQQPIPETCP
ACGVADSLAPCGPGVERLAEEAAHRFPKARMDVAASDTVTGPKEAAALATRIANHDIDLIIGTQIMAKGHHFPLITLVGV
VDGDLGLTGGDLRASERTHQLLHQVAGRAGRAERPGRVLIQTVDPGHPVMEALASGDPALFLEVEAAERQALAMPPFGRL
VALVISGEDSARVQAVAAALGRAAPMGPGLDVLGPVPAPLAMLRGRHRHRLLLKAARGVKVQPVVRHWLSLVSIPPGVKV
QVDVDPISFL

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain

Homologues:

Organism=Escherichia coli, GI1790370, Length=803, Percent_Identity=35.491905354919, Blast_Score=395, Evalue=1e-111,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PRIA_RHORU (P05445)

Other databases:

- EMBL:   X02499
- EMBL:   X02499
- PIR:   S08579
- ProteinModelPortal:   P05445
- SMR:   P05445
- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259
- SMART:   SM00487
- SMART:   SM00490
- TIGRFAMs:   TIGR00595

Pfam domain/function: PF00270 DEAD

EC number: NA

Molecular weight: Translated: 85576; Mature: 85444

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: PS51192 HELICASE_ATP_BIND_1; PS51194 HELICASE_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGLPALPPGSRELFPEDAHAEPVVAVLLPLPLAGAYDYKVPAGMARPAVGTLVRVPLGR
CCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHEEECCCCC
REEIGVVWGAGAGETPPERLKPLIGFPECPPLPAPLRAFIDWVAAYTVQPPGAVLRMALS
CCCCEEEEECCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHH
VPAALEAPPPALGWRRPSAGQRAAGQRAEGQGPLPGGARLSPGRQRVLAVLDDHPGLPFA
CCHHCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCHHHEEEEECCCCCCCCC
GADLAREAAVGPAVVAAMAKAGLLEAVTRSNEWSPQAPDADRPGPLLSADQQAAADGLRT
CHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
ALDQGFSGLLLEGVTGSGKTEVYFEAIAETLRRGRQALVLLPEIALAAQWPRRFADRFGA
HHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHCC
APVQWHSQMGAAARRRAWRAVALGRAPVVVGARSALFLPYPDLGLIIVDEEHDSAFKQEE
CCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEECCCCCCEEEEECCCCHHHHHHC
GVPYNARDMAVVRARLGGFPAVLASATPSLETIENARQGRYRHLVLPRRHGGAEMPEITL
CCCCCHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCCCEEE
LDLRRAPPQKWLPTDFAGPGGSEGLAAPGGANDEAEEQKAPPPSPTASPSPTASPSPMAR
EECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCC
PARLGWLSPPLITAVEETLAAGEQVLLFLNRRGYAPLTLCRSCGHRLKCPRCTAWLVEHR
CCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHCCCCCCCCHHHHHHHHHC
RDGRLRCHHCGYQQPIPETCPACGVADSLAPCGPGVERLAEEAAHRFPKARMDVAASDTV
CCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHCCCCCCCC
TGPKEAAALATRIANHDIDLIIGTQIMAKGHHFPLITLVGVVDGDLGLTGGDLRASERTH
CCHHHHHHHHHHHHCCCCEEEECCHHHHCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHH
QLLHQVAGRAGRAERPGRVLIQTVDPGHPVMEALASGDPALFLEVEAAERQALAMPPFGR
HHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHCCCCEEEEEEECCCCCEECCCCCCC
LVALVISGEDSARVQAVAAALGRAAPMGPGLDVLGPVPAPLAMLRGRHRHRLLLKAARGV
EEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHCCC
KVQPVVRHWLSLVSIPPGVKVQVDVDPISFL
EECHHHHHHHHHHCCCCCCEEEEECCCCCCC
>Mature Secondary Structure 
AGLPALPPGSRELFPEDAHAEPVVAVLLPLPLAGAYDYKVPAGMARPAVGTLVRVPLGR
CCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHEEECCCCC
REEIGVVWGAGAGETPPERLKPLIGFPECPPLPAPLRAFIDWVAAYTVQPPGAVLRMALS
CCCCEEEEECCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHH
VPAALEAPPPALGWRRPSAGQRAAGQRAEGQGPLPGGARLSPGRQRVLAVLDDHPGLPFA
CCHHCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCHHHEEEEECCCCCCCCC
GADLAREAAVGPAVVAAMAKAGLLEAVTRSNEWSPQAPDADRPGPLLSADQQAAADGLRT
CHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
ALDQGFSGLLLEGVTGSGKTEVYFEAIAETLRRGRQALVLLPEIALAAQWPRRFADRFGA
HHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHCC
APVQWHSQMGAAARRRAWRAVALGRAPVVVGARSALFLPYPDLGLIIVDEEHDSAFKQEE
CCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEECCCCCCEEEEECCCCHHHHHHC
GVPYNARDMAVVRARLGGFPAVLASATPSLETIENARQGRYRHLVLPRRHGGAEMPEITL
CCCCCHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCCCEEE
LDLRRAPPQKWLPTDFAGPGGSEGLAAPGGANDEAEEQKAPPPSPTASPSPTASPSPMAR
EECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCC
PARLGWLSPPLITAVEETLAAGEQVLLFLNRRGYAPLTLCRSCGHRLKCPRCTAWLVEHR
CCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHCCCCCCCCHHHHHHHHHC
RDGRLRCHHCGYQQPIPETCPACGVADSLAPCGPGVERLAEEAAHRFPKARMDVAASDTV
CCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHCCCCCCCC
TGPKEAAALATRIANHDIDLIIGTQIMAKGHHFPLITLVGVVDGDLGLTGGDLRASERTH
CCHHHHHHHHHHHHCCCCEEEECCHHHHCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHH
QLLHQVAGRAGRAERPGRVLIQTVDPGHPVMEALASGDPALFLEVEAAERQALAMPPFGR
HHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHCCCCEEEEEEECCCCCEECCCCCCC
LVALVISGEDSARVQAVAAALGRAAPMGPGLDVLGPVPAPLAMLRGRHRHRLLLKAARGV
EEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHCCC
KVQPVVRHWLSLVSIPPGVKVQVDVDPISFL
EECHHHHHHHHHHCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2861810