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The map label for this gene is pspA [H]

Identifier: 83592554

GI number: 83592554

Start: 1438028

End: 1438708

Strand: Reverse

Name: pspA [H]

Synonym: Rru_A1218

Alternate gene names: 83592554

Gene position: 1438708-1438028 (Counterclockwise)

Preceding gene: 83592556

Following gene: 83592553

Centisome position: 33.05

GC content: 64.17

Gene sequence:

>681_bases
ATGGGCATCTTTTCCCGCCTGACCGACATCGTGAACGCCAATCTCAATTCCCTGATCGACCGCGCCGAAGACCCCGAAAA
GATGGCGCGTCTGATGATTCAGGAAATGGAAGACACCCTGGTTGAGGTCCGCACCGCCACCGTGCGCACCATCGCCGATC
GCAAGGACCTGGAACGCTCGCTCGAACGCCTGGAAGCCGGCGGCCGCGAATGGGGCGAGAAGGCGGAATTCGCCCTGGCC
AAGGGCCGCGAGGATCTGGCCAAGGCGGCGTTGATCGCCAAGCGCAAGCTGGCCGATCAGGCCGAAGGGGCGCGGGCCGA
GCTGAAGGCCATCGACGAGAGCCTCGCCAAGACCAATGTCGATCTCGGTCAGCTTCAGGCCAAGCTGGAAGAAGCCAAGG
CCAAGCGCAAATCCCTGGAAATCCGCATGCGTTCGGCGCAAAAGCGGGTAGATTTGCGCCGCACCCTCCACGACGGCCGC
ATCGACGAGGCCCTGGGGCGCTATGACTCGCTTGAACGCCGGATTTCCGAGCTTGAAGCCGACGCCGAAAGCTACGATCT
CGGCAAGACGGCGGGCGTCAGCCTTGATGAGCAGTTTTCCGAACTGCGCGCCGAGGCCGATGTGGAGGACGAACTGGCGC
GGTTGAAGGAGACCGTCGCGCGGCGTGGCGCCGGGGCCTGA

Upstream 100 bases:

>100_bases
TGATTCTCAAGCGTTTTTTCTTTGGCACGCTTCTGGCTAAGCAAAAGGCAGAGCACCCCACGACAGGGGCGCCCCCTTTT
CCCAAGCATGGAGGCCCCAA

Downstream 100 bases:

>100_bases
TCTTAAAGGCTTCGTCCCCGCTCGCGGGGACGCCGCCCGCCCGCCTCGCCCTACCGCCCGCGCCGCCCGCCTGCGCCCCC
GCCTGCGAAGGATTTGATCC

Product: phage shock protein A, PspA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 226; Mature: 225

Protein sequence:

>226_residues
MGIFSRLTDIVNANLNSLIDRAEDPEKMARLMIQEMEDTLVEVRTATVRTIADRKDLERSLERLEAGGREWGEKAEFALA
KGREDLAKAALIAKRKLADQAEGARAELKAIDESLAKTNVDLGQLQAKLEEAKAKRKSLEIRMRSAQKRVDLRRTLHDGR
IDEALGRYDSLERRISELEADAESYDLGKTAGVSLDEQFSELRAEADVEDELARLKETVARRGAGA

Sequences:

>Translated_226_residues
MGIFSRLTDIVNANLNSLIDRAEDPEKMARLMIQEMEDTLVEVRTATVRTIADRKDLERSLERLEAGGREWGEKAEFALA
KGREDLAKAALIAKRKLADQAEGARAELKAIDESLAKTNVDLGQLQAKLEEAKAKRKSLEIRMRSAQKRVDLRRTLHDGR
IDEALGRYDSLERRISELEADAESYDLGKTAGVSLDEQFSELRAEADVEDELARLKETVARRGAGA
>Mature_225_residues
GIFSRLTDIVNANLNSLIDRAEDPEKMARLMIQEMEDTLVEVRTATVRTIADRKDLERSLERLEAGGREWGEKAEFALAK
GREDLAKAALIAKRKLADQAEGARAELKAIDESLAKTNVDLGQLQAKLEEAKAKRKSLEIRMRSAQKRVDLRRTLHDGRI
DEALGRYDSLERRISELEADAESYDLGKTAGVSLDEQFSELRAEADVEDELARLKETVARRGAGA

Specific function: The phage shock protein (psp) operon (pspABCDE) may play a significant role in the competition for survival under nutrient- or energy-limited conditions. PspA negatively regulates expression of the pspABCDE promoter and of pspG through negative regulation

COG id: COG1842

COG function: function code KT; Phage shock protein A (IM30), suppresses sigma54-dependent transcription

Gene ontology:

Cell location: Cytoplasm. Cell inner membrane; Peripheral membrane protein; Cytoplasmic side (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PspA/IM30 family [H]

Homologues:

Organism=Escherichia coli, GI2367118, Length=216, Percent_Identity=50.462962962963, Blast_Score=213, Evalue=8e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014319
- InterPro:   IPR007157 [H]

Pfam domain/function: PF04012 PspA_IM30 [H]

EC number: NA

Molecular weight: Translated: 25278; Mature: 25147

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIFSRLTDIVNANLNSLIDRAEDPEKMARLMIQEMEDTLVEVRTATVRTIADRKDLERS
CCHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LERLEAGGREWGEKAEFALAKGREDLAKAALIAKRKLADQAEGARAELKAIDESLAKTNV
HHHHHHCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
DLGQLQAKLEEAKAKRKSLEIRMRSAQKRVDLRRTLHDGRIDEALGRYDSLERRISELEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
DAESYDLGKTAGVSLDEQFSELRAEADVEDELARLKETVARRGAGA
CHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
GIFSRLTDIVNANLNSLIDRAEDPEKMARLMIQEMEDTLVEVRTATVRTIADRKDLERS
CHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LERLEAGGREWGEKAEFALAKGREDLAKAALIAKRKLADQAEGARAELKAIDESLAKTNV
HHHHHHCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
DLGQLQAKLEEAKAKRKSLEIRMRSAQKRVDLRRTLHDGRIDEALGRYDSLERRISELEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
DAESYDLGKTAGVSLDEQFSELRAEADVEDELARLKETVARRGAGA
CHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]