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The map label for this gene is dapF [H]

Identifier: 83592519

GI number: 83592519

Start: 1400873

End: 1401715

Strand: Reverse

Name: dapF [H]

Synonym: Rru_A1183

Alternate gene names: 83592519

Gene position: 1401715-1400873 (Counterclockwise)

Preceding gene: 83592528

Following gene: 83592518

Centisome position: 32.2

GC content: 67.5

Gene sequence:

>843_bases
ATGAGCATACGTGGCACCCATTTCCTGAAAATGCACGGCCTGGGCAACGATTTTATCGTGATCGACGCCCGGACCCGTCC
GCTCGACCTGACGCCCGAGCGCGTCCGCGCCTTGGCCGACCGGCATTCGGGGGTGGGCTGCGATCAGTTCGTCACCATCG
AACCGGCGCGTGGTGGCGGCGTCGCCTTCATGGGCCTGCGCAACGCCGATGGCGAAATCGTCGAAAGCTGTGGCAACGCC
TCGCGCTGCGTCGGCCGCCTGCTGCTTGAGGAACGCGAAGCCGAAAGCGTTCTGATCGAAACCCTGGGCGGCATGGTCGA
AGCCCGCCGGGCCAGTGGCGAGTTGATCGAGGTCGACATGGGCCCGGCCCGTCTGACCTGGCAAGAGATCCCGCTGGCCG
GGGCGGCCGATACCCTCCACATCGAGCTGAGCGTCGGCCCGTTGAGCGACCCCTGCGCCGTATCGATGGGCAATCCCCAT
GCGGTGTTCTTCGTTGACGACGCCGACGCCATCGACCTCGCCACCTGGGGTCCGCTGATCGAGCACCATGGCCTGTTCCC
CAATCGCACCAATGTCGAAGCCGTTCACCTCCGCGCCGATGGCCGTTTGCGCATGCGGGTATGGGAACGCGGCGTCGGCA
TCACCCGAGCCTGCGGCACCGGCGCCTGCGCCAGCGCCGTGGCCGCCATGCGGCGCGGCTTGATCGCCGGCCGCACCGCC
GAGGTCGTTCTCGATGGCGGCACGCTTGGCATCGTCTGGCGCGAAAGCGACGGCCATGTGCTGATGACCGGCAGTGCCAC
CCTGGCCTATTCGGGGGTCCTGGACGAAGGAGCCTGGGCATGA

Upstream 100 bases:

>100_bases
AGGATTGCGCGTTAGGCAGTACCCGACTTCATCACAGTTTGATCCCGGAATCGGGTGTTCCGCTGGACTCCGTTTCGAGC
CACGCCTATAAGTCGCGATC

Downstream 100 bases:

>100_bases
CCGCCACTCCCCTCGCGCCGCCGACGGCCGATGCGATCGACGATAACGCCAGCCCCGGCGGTCCGCGCATCGTCACTTTC
GGCTGCCGTCTCAATACCTA

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MSIRGTHFLKMHGLGNDFIVIDARTRPLDLTPERVRALADRHSGVGCDQFVTIEPARGGGVAFMGLRNADGEIVESCGNA
SRCVGRLLLEEREAESVLIETLGGMVEARRASGELIEVDMGPARLTWQEIPLAGAADTLHIELSVGPLSDPCAVSMGNPH
AVFFVDDADAIDLATWGPLIEHHGLFPNRTNVEAVHLRADGRLRMRVWERGVGITRACGTGACASAVAAMRRGLIAGRTA
EVVLDGGTLGIVWRESDGHVLMTGSATLAYSGVLDEGAWA

Sequences:

>Translated_280_residues
MSIRGTHFLKMHGLGNDFIVIDARTRPLDLTPERVRALADRHSGVGCDQFVTIEPARGGGVAFMGLRNADGEIVESCGNA
SRCVGRLLLEEREAESVLIETLGGMVEARRASGELIEVDMGPARLTWQEIPLAGAADTLHIELSVGPLSDPCAVSMGNPH
AVFFVDDADAIDLATWGPLIEHHGLFPNRTNVEAVHLRADGRLRMRVWERGVGITRACGTGACASAVAAMRRGLIAGRTA
EVVLDGGTLGIVWRESDGHVLMTGSATLAYSGVLDEGAWA
>Mature_279_residues
SIRGTHFLKMHGLGNDFIVIDARTRPLDLTPERVRALADRHSGVGCDQFVTIEPARGGGVAFMGLRNADGEIVESCGNAS
RCVGRLLLEEREAESVLIETLGGMVEARRASGELIEVDMGPARLTWQEIPLAGAADTLHIELSVGPLSDPCAVSMGNPHA
VFFVDDADAIDLATWGPLIEHHGLFPNRTNVEAVHLRADGRLRMRVWERGVGITRACGTGACASAVAAMRRGLIAGRTAE
VVLDGGTLGIVWRESDGHVLMTGSATLAYSGVLDEGAWA

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=272, Percent_Identity=37.8676470588235, Blast_Score=180, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 29783; Mature: 29652

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIRGTHFLKMHGLGNDFIVIDARTRPLDLTPERVRALADRHSGVGCDQFVTIEPARGGG
CCCCCEEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCC
VAFMGLRNADGEIVESCGNASRCVGRLLLEEREAESVLIETLGGMVEARRASGELIEVDM
EEEEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
GPARLTWQEIPLAGAADTLHIELSVGPLSDPCAVSMGNPHAVFFVDDADAIDLATWGPLI
CCCEEEHHHCCCCCCCCEEEEEEEECCCCCCCEEECCCCEEEEEECCCCEEEEHHCCHHH
EHHGLFPNRTNVEAVHLRADGRLRMRVWERGVGITRACGTGACASAVAAMRRGLIAGRTA
HHCCCCCCCCCEEEEEEECCCEEEEEEECCCCCEEECCCCCHHHHHHHHHHCCHHCCCEE
EVVLDGGTLGIVWRESDGHVLMTGSATLAYSGVLDEGAWA
EEEEECCEEEEEEECCCCCEEEECCCEEEEECCCCCCCCC
>Mature Secondary Structure 
SIRGTHFLKMHGLGNDFIVIDARTRPLDLTPERVRALADRHSGVGCDQFVTIEPARGGG
CCCCEEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCC
VAFMGLRNADGEIVESCGNASRCVGRLLLEEREAESVLIETLGGMVEARRASGELIEVDM
EEEEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
GPARLTWQEIPLAGAADTLHIELSVGPLSDPCAVSMGNPHAVFFVDDADAIDLATWGPLI
CCCEEEHHHCCCCCCCCEEEEEEEECCCCCCCEEECCCCEEEEEECCCCEEEEHHCCHHH
EHHGLFPNRTNVEAVHLRADGRLRMRVWERGVGITRACGTGACASAVAAMRRGLIAGRTA
HHCCCCCCCCCEEEEEEECCCEEEEEEECCCCCEEECCCCCHHHHHHHHHHCCHHCCCEE
EVVLDGGTLGIVWRESDGHVLMTGSATLAYSGVLDEGAWA
EEEEECCEEEEEEECCCCCEEEECCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA