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The map label for this gene is hupB [H]

Identifier: 83592498

GI number: 83592498

Start: 1379541

End: 1381331

Strand: Direct

Name: hupB [H]

Synonym: Rru_A1162

Alternate gene names: 83592498

Gene position: 1379541-1381331 (Clockwise)

Preceding gene: 83592497

Following gene: 83592499

Centisome position: 31.69

GC content: 63.43

Gene sequence:

>1791_bases
ATGAGCGTGACGACGCCCAACGGCTTCACATTGAACGATGCCGGCCGCCGCGTGGTGGTCGATCCGGTGACCCGCATCGA
GGGTCATCTGCGCATCGAGCTCAATGTCGACGAGGCCAATGTCATCCGCAACGCCGTGTCGACGGGAACGATGTGGCGCG
GCATCGAACTGATCCTCAAGGGCCGCGATCCGCGCGACGCCTGGGCCTTTACCCAGCGCATCTGCGGGGTGTGCACGGGA
ACCCACGCCCTGACCAGCGTGCGTGCCATCGAAAACACCCTTGGCATCCAGATCCCCGAAAACGCCAATTCCATCCGCAA
CATCATGCAGCTTTGCCTGCAGGTGCATGACCATCTGGTTCACTTCTATCACCTGCATGCCCTGGATTGGGTCGATGTGG
TTTCGGCGCTTTCGGCCGATCCGAAAGCCACCAGCGCCCTGGCGCAAAGCCTGTCGCCCTGGCCGCTGTCCTCGCCCGGC
TATTTTAGCGACATCAAGGCCAAGCTGACCCGTTTCGTCGAATCCGGGCAGTTGGGGCCGTTCAAGAACGGCTATTGGGG
CCACCCGGCCTATCGCCTGCCGCCCGAAGCCAATCTGATGGCGGTCGCCCATTATCTGGAGGCGCTGGATTTCCAGAAGG
AGATCGTCAAGATCCATGCGGTCTTCGGCGGCAAGAACCCCCATCCCAACTGGCTGGTCGGCGGCGTGCCCTGCGCCATC
AACCTTGATGGCGCCGGCGCCGTCGGCGCAGTCAATATCGAGCGGCTCAATCTGGTGTCGTCGATCATCGACCGCGCCAC
CACCTTCGTCGAACAGGTCTATCTGCCCGATCTGATCGCCATCGCCGGCTTCTATAAGGACTGGCAGTATGGCGGCGGCC
TCAGTTCCACCTCGGTGCTGGCCTATGGCGATATCCCCGATCGCGCCAATGATTATTCGCCTTCGAACCTTTTGATGCCG
AGCGGGGCGATCATCAATGGCGAACTGACCCGCATCCATCCGGTTGATCTGACCGACCCCGAGCAGATCCAGGAGTTCGT
TTCCCATTCCTGGTACCGCTATGGCGACGAGGCCAAGGGATTGCACCCCTGGGATGGCGTCACCGAAAGCCACTTCGAGC
TGGGGCCGCGCAGCAAGGTCGAAAACCGGCGCATCGAGACCCTCGATGAAAGCGCCAAATACAGCTACCTCAAGGCGCCG
CGCTGGCGGGGCCATGCCATGGAGGTCGGGCCGCTCGCCCGCTATATCCTGGGCTATGCCCAGGGCAATCCCGAGTTCAA
GGAGCCGACCGAGGCCCTGTTGCGCCAGCTTGACCTGCCGGTCGGCGCCTTGTTCTCCACCCTGGGGCGGACGGCGGCGC
GCGGCCTGGAATGCCAATGGGCGGCGGGCAAGCTGCGCTATTTCTTCGATAAACTGATGGCCAACCTCAAGGCCGGCGAT
CTCAACACCGCCAATACGACGAAATGGGCGCCCGCCACTTGGCCAGCTTCGGCCAAAGGTGTGGGCTTTACCGAAGCGCC
GCGCGGCGCCCTGGCCCATTGGGTCCAGATCGAGGCGGGCAAGATCGCCAATTACCAATGCGTGGTGCCGACCACCTGGA
ACGGCAGTCCGCGCGATCCGGCGGGGGCGATCGGCGCCTTCGAGGCGGCCTTGCTCGATACGCCGCTGGCCGATCCCCAA
CAGCCGCTTGAAGTGCTGCGGACCCTGCACAGCTTTGACCCCTGCCTCGCCTGTTCGACCCATATCCTGACGCCCGAGGG
CGGCGAGGCGATTTCGGTGACGGTGCGCTAA

Upstream 100 bases:

>100_bases
TGGTGGCGGCCGGGGTGGCGGCCCATGCCGGCGTCACCGTGGCCCGGCGCCTGATGTCGAAGAACGAAAACAAAGACAAA
GAGTAGGGGGGGAAAGATCC

Downstream 100 bases:

>100_bases
CGCGTTTTCACGCCAACGGGGAAGGAACGACCATGAGCACCGTGACCGACGAAGCCGCGCCCGAGGTGATCCTGGGCACG
ACGCGCCACCCCTCGGTTTA

Product: nickel-dependent hydrogenase, large subunit

Products: NA

Alternate protein names: Hydrogenlyase; Membrane-bound hydrogenase large subunit [H]

Number of amino acids: Translated: 596; Mature: 595

Protein sequence:

>596_residues
MSVTTPNGFTLNDAGRRVVVDPVTRIEGHLRIELNVDEANVIRNAVSTGTMWRGIELILKGRDPRDAWAFTQRICGVCTG
THALTSVRAIENTLGIQIPENANSIRNIMQLCLQVHDHLVHFYHLHALDWVDVVSALSADPKATSALAQSLSPWPLSSPG
YFSDIKAKLTRFVESGQLGPFKNGYWGHPAYRLPPEANLMAVAHYLEALDFQKEIVKIHAVFGGKNPHPNWLVGGVPCAI
NLDGAGAVGAVNIERLNLVSSIIDRATTFVEQVYLPDLIAIAGFYKDWQYGGGLSSTSVLAYGDIPDRANDYSPSNLLMP
SGAIINGELTRIHPVDLTDPEQIQEFVSHSWYRYGDEAKGLHPWDGVTESHFELGPRSKVENRRIETLDESAKYSYLKAP
RWRGHAMEVGPLARYILGYAQGNPEFKEPTEALLRQLDLPVGALFSTLGRTAARGLECQWAAGKLRYFFDKLMANLKAGD
LNTANTTKWAPATWPASAKGVGFTEAPRGALAHWVQIEAGKIANYQCVVPTTWNGSPRDPAGAIGAFEAALLDTPLADPQ
QPLEVLRTLHSFDPCLACSTHILTPEGGEAISVTVR

Sequences:

>Translated_596_residues
MSVTTPNGFTLNDAGRRVVVDPVTRIEGHLRIELNVDEANVIRNAVSTGTMWRGIELILKGRDPRDAWAFTQRICGVCTG
THALTSVRAIENTLGIQIPENANSIRNIMQLCLQVHDHLVHFYHLHALDWVDVVSALSADPKATSALAQSLSPWPLSSPG
YFSDIKAKLTRFVESGQLGPFKNGYWGHPAYRLPPEANLMAVAHYLEALDFQKEIVKIHAVFGGKNPHPNWLVGGVPCAI
NLDGAGAVGAVNIERLNLVSSIIDRATTFVEQVYLPDLIAIAGFYKDWQYGGGLSSTSVLAYGDIPDRANDYSPSNLLMP
SGAIINGELTRIHPVDLTDPEQIQEFVSHSWYRYGDEAKGLHPWDGVTESHFELGPRSKVENRRIETLDESAKYSYLKAP
RWRGHAMEVGPLARYILGYAQGNPEFKEPTEALLRQLDLPVGALFSTLGRTAARGLECQWAAGKLRYFFDKLMANLKAGD
LNTANTTKWAPATWPASAKGVGFTEAPRGALAHWVQIEAGKIANYQCVVPTTWNGSPRDPAGAIGAFEAALLDTPLADPQ
QPLEVLRTLHSFDPCLACSTHILTPEGGEAISVTVR
>Mature_595_residues
SVTTPNGFTLNDAGRRVVVDPVTRIEGHLRIELNVDEANVIRNAVSTGTMWRGIELILKGRDPRDAWAFTQRICGVCTGT
HALTSVRAIENTLGIQIPENANSIRNIMQLCLQVHDHLVHFYHLHALDWVDVVSALSADPKATSALAQSLSPWPLSSPGY
FSDIKAKLTRFVESGQLGPFKNGYWGHPAYRLPPEANLMAVAHYLEALDFQKEIVKIHAVFGGKNPHPNWLVGGVPCAIN
LDGAGAVGAVNIERLNLVSSIIDRATTFVEQVYLPDLIAIAGFYKDWQYGGGLSSTSVLAYGDIPDRANDYSPSNLLMPS
GAIINGELTRIHPVDLTDPEQIQEFVSHSWYRYGDEAKGLHPWDGVTESHFELGPRSKVENRRIETLDESAKYSYLKAPR
WRGHAMEVGPLARYILGYAQGNPEFKEPTEALLRQLDLPVGALFSTLGRTAARGLECQWAAGKLRYFFDKLMANLKAGDL
NTANTTKWAPATWPASAKGVGFTEAPRGALAHWVQIEAGKIANYQCVVPTTWNGSPRDPAGAIGAFEAALLDTPLADPQQ
PLEVLRTLHSFDPCLACSTHILTPEGGEAISVTVR

Specific function: This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions [H]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787207, Length=590, Percent_Identity=63.8983050847458, Blast_Score=812, Evalue=0.0,
Organism=Escherichia coli, GI1789368, Length=595, Percent_Identity=45.2100840336134, Blast_Score=495, Evalue=1e-141,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 65220; Mature: 65088

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVTTPNGFTLNDAGRRVVVDPVTRIEGHLRIELNVDEANVIRNAVSTGTMWRGIELILK
CCCCCCCCEEECCCCCEEEECCHHHCCCEEEEEEECCHHHHHHHHHHCCCHHHCEEEEEE
GRDPRDAWAFTQRICGVCTGTHALTSVRAIENTLGIQIPENANSIRNIMQLCLQVHDHLV
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCEEECCCHHHHHHHHHHHHHHHHHHH
HFYHLHALDWVDVVSALSADPKATSALAQSLSPWPLSSPGYFSDIKAKLTRFVESGQLGP
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC
FKNGYWGHPAYRLPPEANLMAVAHYLEALDFQKEIVKIHAVFGGKNPHPNWLVGGVPCAI
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEHHEECCCCCCCCEEEECCEEEE
NLDGAGAVGAVNIERLNLVSSIIDRATTFVEQVYLPDLIAIAGFYKDWQYGGGLSSTSVL
EECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
AYGDIPDRANDYSPSNLLMPSGAIINGELTRIHPVDLTDPEQIQEFVSHSWYRYGDEAKG
EECCCCCCCCCCCCCCEECCCCCEECCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCC
LHPWDGVTESHFELGPRSKVENRRIETLDESAKYSYLKAPRWRGHAMEVGPLARYILGYA
CCCCCCCCCHHHCCCCCHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHC
QGNPEFKEPTEALLRQLDLPVGALFSTLGRTAARGLECQWAAGKLRYFFDKLMANLKAGD
CCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCCCC
LNTANTTKWAPATWPASAKGVGFTEAPRGALAHWVQIEAGKIANYQCVVPTTWNGSPRDP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHEEEEEECCCCCCEEEEEEECCCCCCCCC
AGAIGAFEAALLDTPLADPQQPLEVLRTLHSFDPCLACSTHILTPEGGEAISVTVR
CCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHCCCEEECCCCCCEEEEEEC
>Mature Secondary Structure 
SVTTPNGFTLNDAGRRVVVDPVTRIEGHLRIELNVDEANVIRNAVSTGTMWRGIELILK
CCCCCCCEEECCCCCEEEECCHHHCCCEEEEEEECCHHHHHHHHHHCCCHHHCEEEEEE
GRDPRDAWAFTQRICGVCTGTHALTSVRAIENTLGIQIPENANSIRNIMQLCLQVHDHLV
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCEEECCCHHHHHHHHHHHHHHHHHHH
HFYHLHALDWVDVVSALSADPKATSALAQSLSPWPLSSPGYFSDIKAKLTRFVESGQLGP
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC
FKNGYWGHPAYRLPPEANLMAVAHYLEALDFQKEIVKIHAVFGGKNPHPNWLVGGVPCAI
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEHHEECCCCCCCCEEEECCEEEE
NLDGAGAVGAVNIERLNLVSSIIDRATTFVEQVYLPDLIAIAGFYKDWQYGGGLSSTSVL
EECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
AYGDIPDRANDYSPSNLLMPSGAIINGELTRIHPVDLTDPEQIQEFVSHSWYRYGDEAKG
EECCCCCCCCCCCCCCEECCCCCEECCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCC
LHPWDGVTESHFELGPRSKVENRRIETLDESAKYSYLKAPRWRGHAMEVGPLARYILGYA
CCCCCCCCCHHHCCCCCHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHC
QGNPEFKEPTEALLRQLDLPVGALFSTLGRTAARGLECQWAAGKLRYFFDKLMANLKAGD
CCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCCCC
LNTANTTKWAPATWPASAKGVGFTEAPRGALAHWVQIEAGKIANYQCVVPTTWNGSPRDP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHEEEEEECCCCCCEEEEEEECCCCCCCCC
AGAIGAFEAALLDTPLADPQQPLEVLRTLHSFDPCLACSTHILTPEGGEAISVTVR
CCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHCCCEEECCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2402452; 2103457 [H]