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The map label for this gene is glyA

Identifier: 83592478

GI number: 83592478

Start: 1347656

End: 1348960

Strand: Reverse

Name: glyA

Synonym: Rru_A1142

Alternate gene names: 83592478

Gene position: 1348960-1347656 (Counterclockwise)

Preceding gene: 83592479

Following gene: 83592477

Centisome position: 30.99

GC content: 66.28

Gene sequence:

>1305_bases
ATGGAAGCATCGGTCGCGCCCCCCGCACTCCGGAACGCCCCCTGCGCAGGCGTCAACGCCGCCTGTTTGTCCGCCGCCGA
TGACGCCGTTGCCATCGCCATCGCCCAGGAGACGACACGCCAGCGCGAGTCCATCGAGTTGATCGCCTCGGAGAACTTTG
TGAGCAAGGCGGTGCTCGAGGCGCAGGGGTCCGTGCTCACCAACAAATATGCCGAGGGCTATCCGCAGCGCCGCTATTAT
GGCGGATGCGCGAACGTCGATAGGGTCGAGGATCTGGCGATCGCCCGCCTCAACCAGCTTTTCGGCAGCACCTACGCCAA
CGTCCAGCCCCATTCGGGCAGCCAAGCCAATCAGGCGGTCTTTTTGGCGCTTCTCGCCCCCGGTGACACCATCCTGGGGC
TCGACCTTAAGGCCGGCGGCCATCTCACCCATGGCGCCCCGGTCAATATCTCCGGCCGTTGGTTCACGGCCGTCAGCTAT
GGCGTGGACCCGCGAACCCATCTCATCGACATGGAGCAGATGGCGGACCTTGCCCGCCGGCACCGGCCGAAGCTGCTGAT
CGCCGGCGGGTCGGCCTATCCGCGCCTCCTGGACTTTGCGCGCTTCCGCCAGATCGCCGACGAGGTGGGGGCGATCTTGA
TGGTCGATATGGCGCATTTCGCCGGGTTGGTCGCTGGCGGGGTGTATCCGTCGCCGGTGCCCTTCGCTGATGTCATCACC
TCGACCACGCACAAGACGCTGCGCGGGCCGCGGGGCGGGTTCGTCCTGACCAATGACGCCGCGATCGCCAAGAAGATCAA
CTCGGCGGTCTTTCCCGGCCTGCAAGGCGGTCCGCTCATGCACATCATCGCGGCCAAGGCCGTGGCGTTTGGCGAGGCGC
TCGATCCCTCGTTCAAGATCTATGCGCGGCGTGTCGTCGAGAATTGCCGCGTTCTCGCCCAAACGCTCCTCGACGGCGGA
CTGGCGATCACCAGTGGCGGGACCGACTGCCATCTGGCGGTGGTCGATCTGCGCCCCCTTGGTGTCACCGGTACCATCGC
CGAACAGGCCCTGGAATCGATCGGCATCACCCTCAACAAGAATGCGATCCCCAACGATCCCGAAAAGCCGATGGTGACCT
CGGGCATCCGGGTCGGCAGCGCGGCGGGAACCTCGCGCGGGTTTGGACCCGAGGAGTATCGGCGGATCGCCGCGTTGATC
CTCGAGACGCTGCACGCTGTGCGCGCCGGGACGCTCGAGGCCGACCGGGAGGGCATCCGCACCCGGGTGCGCAGCCTCGT
GGCGGGTTTTCCCCTGCCTTACTGA

Upstream 100 bases:

>100_bases
ATGATTAGGTGCTAACGGAAGCCGCCGCGTCAAACCGGCGCGGCGCAGGGCCCCTTACCAGGATCAGGCCCTTACCAGCA
TCAACAAGGAGAGATCCGTG

Downstream 100 bases:

>100_bases
CCACCACACCCGGGGAGCCCGTTTTGCTATGACAGACTCATTGACCGTCGATCTTTCAGAGGAAGACGTGGCGCGCTTCG
GGACGGATCCCGCCCGCGCC

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT 1; Serine methylase 1

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MEASVAPPALRNAPCAGVNAACLSAADDAVAIAIAQETTRQRESIELIASENFVSKAVLEAQGSVLTNKYAEGYPQRRYY
GGCANVDRVEDLAIARLNQLFGSTYANVQPHSGSQANQAVFLALLAPGDTILGLDLKAGGHLTHGAPVNISGRWFTAVSY
GVDPRTHLIDMEQMADLARRHRPKLLIAGGSAYPRLLDFARFRQIADEVGAILMVDMAHFAGLVAGGVYPSPVPFADVIT
STTHKTLRGPRGGFVLTNDAAIAKKINSAVFPGLQGGPLMHIIAAKAVAFGEALDPSFKIYARRVVENCRVLAQTLLDGG
LAITSGGTDCHLAVVDLRPLGVTGTIAEQALESIGITLNKNAIPNDPEKPMVTSGIRVGSAAGTSRGFGPEEYRRIAALI
LETLHAVRAGTLEADREGIRTRVRSLVAGFPLPY

Sequences:

>Translated_434_residues
MEASVAPPALRNAPCAGVNAACLSAADDAVAIAIAQETTRQRESIELIASENFVSKAVLEAQGSVLTNKYAEGYPQRRYY
GGCANVDRVEDLAIARLNQLFGSTYANVQPHSGSQANQAVFLALLAPGDTILGLDLKAGGHLTHGAPVNISGRWFTAVSY
GVDPRTHLIDMEQMADLARRHRPKLLIAGGSAYPRLLDFARFRQIADEVGAILMVDMAHFAGLVAGGVYPSPVPFADVIT
STTHKTLRGPRGGFVLTNDAAIAKKINSAVFPGLQGGPLMHIIAAKAVAFGEALDPSFKIYARRVVENCRVLAQTLLDGG
LAITSGGTDCHLAVVDLRPLGVTGTIAEQALESIGITLNKNAIPNDPEKPMVTSGIRVGSAAGTSRGFGPEEYRRIAALI
LETLHAVRAGTLEADREGIRTRVRSLVAGFPLPY
>Mature_434_residues
MEASVAPPALRNAPCAGVNAACLSAADDAVAIAIAQETTRQRESIELIASENFVSKAVLEAQGSVLTNKYAEGYPQRRYY
GGCANVDRVEDLAIARLNQLFGSTYANVQPHSGSQANQAVFLALLAPGDTILGLDLKAGGHLTHGAPVNISGRWFTAVSY
GVDPRTHLIDMEQMADLARRHRPKLLIAGGSAYPRLLDFARFRQIADEVGAILMVDMAHFAGLVAGGVYPSPVPFADVIT
STTHKTLRGPRGGFVLTNDAAIAKKINSAVFPGLQGGPLMHIIAAKAVAFGEALDPSFKIYARRVVENCRVLAQTLLDGG
LAITSGGTDCHLAVVDLRPLGVTGTIAEQALESIGITLNKNAIPNDPEKPMVTSGIRVGSAAGTSRGFGPEEYRRIAALI
LETLHAVRAGTLEADREGIRTRVRSLVAGFPLPY

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI261862352, Length=452, Percent_Identity=44.9115044247788, Blast_Score=350, Evalue=1e-96,
Organism=Homo sapiens, GI261862350, Length=452, Percent_Identity=44.9115044247788, Blast_Score=350, Evalue=1e-96,
Organism=Homo sapiens, GI261862348, Length=452, Percent_Identity=44.9115044247788, Blast_Score=350, Evalue=1e-96,
Organism=Homo sapiens, GI19923315, Length=452, Percent_Identity=44.9115044247788, Blast_Score=350, Evalue=1e-96,
Organism=Homo sapiens, GI261862346, Length=452, Percent_Identity=43.5840707964602, Blast_Score=330, Evalue=1e-90,
Organism=Homo sapiens, GI22547186, Length=455, Percent_Identity=44.3956043956044, Blast_Score=323, Evalue=2e-88,
Organism=Homo sapiens, GI22547189, Length=441, Percent_Identity=41.7233560090703, Blast_Score=289, Evalue=4e-78,
Organism=Escherichia coli, GI1788902, Length=408, Percent_Identity=54.4117647058823, Blast_Score=427, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI25144732, Length=416, Percent_Identity=45.1923076923077, Blast_Score=336, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI25144729, Length=416, Percent_Identity=45.1923076923077, Blast_Score=335, Evalue=2e-92,
Organism=Saccharomyces cerevisiae, GI6319739, Length=401, Percent_Identity=44.8877805486284, Blast_Score=338, Evalue=7e-94,
Organism=Saccharomyces cerevisiae, GI6323087, Length=401, Percent_Identity=43.640897755611, Blast_Score=320, Evalue=3e-88,
Organism=Drosophila melanogaster, GI24640005, Length=453, Percent_Identity=44.1501103752759, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI221329721, Length=453, Percent_Identity=44.1501103752759, Blast_Score=361, Evalue=1e-100,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA1_RHORT (Q2RVA2)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_426230.1
- ProteinModelPortal:   Q2RVA2
- SMR:   Q2RVA2
- STRING:   Q2RVA2
- GeneID:   3834652
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A1142
- NMPDR:   fig|1085.1.peg.3228
- eggNOG:   COG0112
- HOGENOM:   HBG301263
- OMA:   YGGCANV
- ProtClustDB:   PRK13034
- BioCyc:   RRUB269796:RRU_A1142-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 45849; Mature: 45849

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEASVAPPALRNAPCAGVNAACLSAADDAVAIAIAQETTRQRESIELIASENFVSKAVLE
CCCCCCCCCCCCCCCCCCCHHHHHCCCCEEEEEEEHHHHHHHHHEEEEECCCHHHHHHHH
AQGSVLTNKYAEGYPQRRYYGGCANVDRVEDLAIARLNQLFGSTYANVQPHSGSQANQAV
HCCCHHHHHHHCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEE
FLALLAPGDTILGLDLKAGGHLTHGAPVNISGRWFTAVSYGVDPRTHLIDMEQMADLARR
EEEEECCCCEEEEEEECCCCCCCCCCCEECCCCEEEEEEECCCCCHHEECHHHHHHHHHH
HRPKLLIAGGSAYPRLLDFARFRQIADEVGAILMVDMAHFAGLVAGGVYPSPVPFADVIT
CCCEEEEECCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
STTHKTLRGPRGGFVLTNDAAIAKKINSAVFPGLQGGPLMHIIAAKAVAFGEALDPSFKI
HHHHHHHCCCCCCEEEECCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHHH
YARRVVENCRVLAQTLLDGGLAITSGGTDCHLAVVDLRPLGVTGTIAEQALESIGITLNK
HHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEEEECCCCCHHHHHHHHHHCCCEEEC
NAIPNDPEKPMVTSGIRVGSAAGTSRGFGPEEYRRIAALILETLHAVRAGTLEADREGIR
CCCCCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
TRVRSLVAGFPLPY
HHHHHHHHCCCCCC
>Mature Secondary Structure
MEASVAPPALRNAPCAGVNAACLSAADDAVAIAIAQETTRQRESIELIASENFVSKAVLE
CCCCCCCCCCCCCCCCCCCHHHHHCCCCEEEEEEEHHHHHHHHHEEEEECCCHHHHHHHH
AQGSVLTNKYAEGYPQRRYYGGCANVDRVEDLAIARLNQLFGSTYANVQPHSGSQANQAV
HCCCHHHHHHHCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEE
FLALLAPGDTILGLDLKAGGHLTHGAPVNISGRWFTAVSYGVDPRTHLIDMEQMADLARR
EEEEECCCCEEEEEEECCCCCCCCCCCEECCCCEEEEEEECCCCCHHEECHHHHHHHHHH
HRPKLLIAGGSAYPRLLDFARFRQIADEVGAILMVDMAHFAGLVAGGVYPSPVPFADVIT
CCCEEEEECCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
STTHKTLRGPRGGFVLTNDAAIAKKINSAVFPGLQGGPLMHIIAAKAVAFGEALDPSFKI
HHHHHHHCCCCCCEEEECCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHHH
YARRVVENCRVLAQTLLDGGLAITSGGTDCHLAVVDLRPLGVTGTIAEQALESIGITLNK
HHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEEEECCCCCHHHHHHHHHHCCCEEEC
NAIPNDPEKPMVTSGIRVGSAAGTSRGFGPEEYRRIAALILETLHAVRAGTLEADREGIR
CCCCCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
TRVRSLVAGFPLPY
HHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA