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The map label for this gene is ilvE [H]

Identifier: 83592467

GI number: 83592467

Start: 1332809

End: 1333675

Strand: Reverse

Name: ilvE [H]

Synonym: Rru_A1131

Alternate gene names: 83592467

Gene position: 1333675-1332809 (Counterclockwise)

Preceding gene: 83592468

Following gene: 83592466

Centisome position: 30.64

GC content: 68.05

Gene sequence:

>867_bases
ATGACCGCCCGGCGACCGGCCGTCATTTGGCTGAACGACCGGCTCGTGCCCGCCGCAAAGGCCCGCATCGATCCCGCCGA
TCGCGGATTTCTGCTTGGCGATGGCCTATTCGAAACCATCCCGGCGCGCGACGGCCGCCCCCTGCGTCTGGCCGCCCACC
TCGCCCGCCTGGGGCGCGGCGCCCGAATTTTAGGCATTCCCCTACCCGCCCTGGATATCGCCGCCGCCTTGGCCGCCACC
CTGGCGGCCAACGACCTGAGCGAGGGGGTGCTGCGCCTGACCCTGACCCGGGGTCCCGGGCCGCGCGGCCTGTTGCCGCC
GCCCGCGCCAAAGCCCACGATCATGATCACGGCGACGGCCTTTCCGCCGCCAACGGGGCCGGCGCGGCTGATCGTGGCGA
CGCGGACAAGGCGCAACGAGGCCTCGCCGCTGTCGACGATCAAATATCTTGCCTATGGCGACGCCATCCTCGCCCGCCAG
GAGGCCGCCGAGCGCGGCGCCGATGACGCGATCTTGCTCAACCTCGGGGGTCGGGTCGCCGAAACCACCGTCGCCACGCT
GTTCATCGTCCAGGGCGGGCGGCTTCTCACCCCTCCGCAGACCGACGGCGCCCTGCCCGGCATTCTTCGCGCCGAGATGC
TGGCCTGGGGAGCGCGCGAACACTCGCTGACCCCCGCCGATCTTTTGACCGCCGACGGCGTTTTTCTGGCCAACAGCCTT
GGGTTCCGCTTGGTCATTTCCATTGACGGACAGGCCGTTCCCGATTGCACACCCCTTGTTCATGACCTTCAAGGCTATGT
AAGACAAAGGGAAAATGGCACCTCACCGGAAGAGCCCTCTGATGGGGGAAGCCCCTTCCCGAAATAA

Upstream 100 bases:

>100_bases
CCGGCGGCGCCATCACCGCGCCCTCCGATCCGGCCGAGGAATACGAGGAAAGCCTGATCAAAATGGCCCCCCTGCTCAGC
GCCCTGGATGGGATCGAGCG

Downstream 100 bases:

>100_bases
CAAACCGTGCAAATAGGTTTTTAATCTTGCCAAGGTCGCGCTTGACCGCTACTGTTAAGATGACGATTGCCGAAGGCCGC
CCTTCTGGTCCGCCTTCCAT

Product: branched chain amino acid: 2-keto-4-methylthiobutyrate aminotransferase

Products: NA

Alternate protein names: BCAT; Transaminase B [H]

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MTARRPAVIWLNDRLVPAAKARIDPADRGFLLGDGLFETIPARDGRPLRLAAHLARLGRGARILGIPLPALDIAAALAAT
LAANDLSEGVLRLTLTRGPGPRGLLPPPAPKPTIMITATAFPPPTGPARLIVATRTRRNEASPLSTIKYLAYGDAILARQ
EAAERGADDAILLNLGGRVAETTVATLFIVQGGRLLTPPQTDGALPGILRAEMLAWGAREHSLTPADLLTADGVFLANSL
GFRLVISIDGQAVPDCTPLVHDLQGYVRQRENGTSPEEPSDGGSPFPK

Sequences:

>Translated_288_residues
MTARRPAVIWLNDRLVPAAKARIDPADRGFLLGDGLFETIPARDGRPLRLAAHLARLGRGARILGIPLPALDIAAALAAT
LAANDLSEGVLRLTLTRGPGPRGLLPPPAPKPTIMITATAFPPPTGPARLIVATRTRRNEASPLSTIKYLAYGDAILARQ
EAAERGADDAILLNLGGRVAETTVATLFIVQGGRLLTPPQTDGALPGILRAEMLAWGAREHSLTPADLLTADGVFLANSL
GFRLVISIDGQAVPDCTPLVHDLQGYVRQRENGTSPEEPSDGGSPFPK
>Mature_287_residues
TARRPAVIWLNDRLVPAAKARIDPADRGFLLGDGLFETIPARDGRPLRLAAHLARLGRGARILGIPLPALDIAAALAATL
AANDLSEGVLRLTLTRGPGPRGLLPPPAPKPTIMITATAFPPPTGPARLIVATRTRRNEASPLSTIKYLAYGDAILARQE
AAERGADDAILLNLGGRVAETTVATLFIVQGGRLLTPPQTDGALPGILRAEMLAWGAREHSLTPADLLTADGVFLANSLG
FRLVISIDGQAVPDCTPLVHDLQGYVRQRENGTSPEEPSDGGSPFPK

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=287, Percent_Identity=28.2229965156794, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787338, Length=225, Percent_Identity=28.8888888888889, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 30324; Mature: 30193

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARRPAVIWLNDRLVPAAKARIDPADRGFLLGDGLFETIPARDGRPLRLAAHLARLGRG
CCCCCCEEEEECCCCCCHHHHCCCCCCCCEEEECCHHHHCCCCCCCCHHHHHHHHHHCCC
ARILGIPLPALDIAAALAATLAANDLSEGVLRLTLTRGPGPRGLLPPPAPKPTIMITATA
CEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEEEC
FPPPTGPARLIVATRTRRNEASPLSTIKYLAYGDAILARQEAAERGADDAILLNLGGRVA
CCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEECCCCHH
ETTVATLFIVQGGRLLTPPQTDGALPGILRAEMLAWGAREHSLTPADLLTADGVFLANSL
HHHEEEEEEEECCEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHCCCCEEEECCC
GFRLVISIDGQAVPDCTPLVHDLQGYVRQRENGTSPEEPSDGGSPFPK
CEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TARRPAVIWLNDRLVPAAKARIDPADRGFLLGDGLFETIPARDGRPLRLAAHLARLGRG
CCCCCEEEEECCCCCCHHHHCCCCCCCCEEEECCHHHHCCCCCCCCHHHHHHHHHHCCC
ARILGIPLPALDIAAALAATLAANDLSEGVLRLTLTRGPGPRGLLPPPAPKPTIMITATA
CEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEEEC
FPPPTGPARLIVATRTRRNEASPLSTIKYLAYGDAILARQEAAERGADDAILLNLGGRVA
CCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEECCCCHH
ETTVATLFIVQGGRLLTPPQTDGALPGILRAEMLAWGAREHSLTPADLLTADGVFLANSL
HHHEEEEEEEECCEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHCCCCEEEECCC
GFRLVISIDGQAVPDCTPLVHDLQGYVRQRENGTSPEEPSDGGSPFPK
CEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]