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The map label for this gene is tilS [H]

Identifier: 83592433

GI number: 83592433

Start: 1286212

End: 1287600

Strand: Direct

Name: tilS [H]

Synonym: Rru_A1097

Alternate gene names: 83592433

Gene position: 1286212-1287600 (Clockwise)

Preceding gene: 83592432

Following gene: 83592434

Centisome position: 29.55

GC content: 73.79

Gene sequence:

>1389_bases
ATGGCGCCCCGTGGGCTGGTCGACGCGAACGGGGTCATCGACGCCGAGGCTTTCGCCGCCCGCATGGCCGCCCTTGGTCC
CTTCGAGCCCGAGCCGGCGATCGCCGTCGGGGTTTCGGGCGGCGTTGACAGCCTGACCCTTCTCGCCTTCGCCGCCCGCT
GGGCGGCCGAGCGGGGAGGGCGCTGTACGGCGGTTTGCTTCGACCACGGGTTACGCGCCGCCTCGGCCGGCGAGGCGCGG
CTGGTCGAAACGACGGCGCGCAGCCTGGGCGCCGAGGCGGTGGTGCTGCGCTGGGCCGGGCCCCCTCCCTCCTCGGGGGT
TCAGGAAGCCGCCCGCCGCGCCCGGCTTGACGGCTTGCTGGGCTGGTGCGCCGGCGCCGGGGTTCTGCATCTTCTTCTGG
CCCATCACCGCGATGATCAGGCCGAAACCGTGCTCCAGCGCCTGGGGCGCGGCAGCGGCGCCGACGGATTGGCGGCGATG
GCCGCCCTGTCGTCGCGGGGCGAGGCGCGGATCCTGCGCCCGTTTCTAGAGGTGCCGCGCGCGGCGATCGAAGCCAGCGC
CCGCCGGATCGGGCTGGTTTGGGCCGAAGACCCCAGCAACCACGACCCCCATTACGACCGCGCCCGTTTGCGCGCCGCCG
CCGCTCCGCTTGACCAAGCGGGGTTGAGCGCCAAGGCCCTGGCGGCGAGCGCGGCGCGGCTGGGCGATGATCGCGCCTTT
TGGGCCCGCCACCTGTCGGCGCTGCTCGCCGGCGCCACCCGCGCGTGTGGCGAGGGCTGGCTGGTTCTCGACCGCGACGC
CCTGGCGGCAAGCGATCCCTGGGCCGCCGCCCGCGCCCTCGCCTGGGCCGCCGGCGCCATCGCCGGCAGCGAACGCGCCG
CCGATCCCCGGCCGATCGCCGCCTTGCTGGCGACCCCGCCCGCTCCCGGTCAGGTCCACGCGCTGGCGGGCTGTTTGTGG
CAAAGCAAACCGCGCGGCGCCCTGGTGCTGTGTCGCGAATGCCGGGGGCTCGATCGCCGCCGCTGGGCGGTGCCGCCGGG
CGAAAGCCTGCTGTGGGATGGCCGCTGGCTTTGCCGGGCCGGACGGGGAGGGCTGGCGGTCTCGGCCCTGGGGGCAGCGG
GCTGGCAGAAGCTGGCGCCGGCCCTGGCCGGGGACCGCCAAGGCCTGCCGCCACCTATCGCCCTGCCGGCCCTGCCGATT
TTTTATGGCGAGTCGGCGGAGGAGAGGGCGGACGTTTTCGCGGTTCCGTCTCTAGGCCTTTGGGGGTATGGTTACAATCC
GGGGTCGGCTTTTCGGGGCGAAATGCCCTTTAGCGTCGATATCACCTATCATCCCTTTCTACCGCTCGTTCCCCTCGGCC
TGCGGGTCGGTGCCGGCGGCGGCTTGTAA

Upstream 100 bases:

>100_bases
TTCGGCCGCCTCGAGTCGCAATACCCCAAGGCCGCCGACGCCATCAAGCGCCGGGCCAAGGAAGGCATGTCCAAGCTCGG
CTGCTAAGCGGGCCGAGGCC

Downstream 100 bases:

>100_bases
GAGGGGTCCCCTGGAAAATGGCGGCTTGAGGGATGGTTGACCCACCCTTACATTAGCCGCCACGGGGTCTTGTTGGACCT
ACGGCTGATCATCGGCGCAG

Product: PP-loop protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MAPRGLVDANGVIDAEAFAARMAALGPFEPEPAIAVGVSGGVDSLTLLAFAARWAAERGGRCTAVCFDHGLRAASAGEAR
LVETTARSLGAEAVVLRWAGPPPSSGVQEAARRARLDGLLGWCAGAGVLHLLLAHHRDDQAETVLQRLGRGSGADGLAAM
AALSSRGEARILRPFLEVPRAAIEASARRIGLVWAEDPSNHDPHYDRARLRAAAAPLDQAGLSAKALAASAARLGDDRAF
WARHLSALLAGATRACGEGWLVLDRDALAASDPWAAARALAWAAGAIAGSERAADPRPIAALLATPPAPGQVHALAGCLW
QSKPRGALVLCRECRGLDRRRWAVPPGESLLWDGRWLCRAGRGGLAVSALGAAGWQKLAPALAGDRQGLPPPIALPALPI
FYGESAEERADVFAVPSLGLWGYGYNPGSAFRGEMPFSVDITYHPFLPLVPLGLRVGAGGGL

Sequences:

>Translated_462_residues
MAPRGLVDANGVIDAEAFAARMAALGPFEPEPAIAVGVSGGVDSLTLLAFAARWAAERGGRCTAVCFDHGLRAASAGEAR
LVETTARSLGAEAVVLRWAGPPPSSGVQEAARRARLDGLLGWCAGAGVLHLLLAHHRDDQAETVLQRLGRGSGADGLAAM
AALSSRGEARILRPFLEVPRAAIEASARRIGLVWAEDPSNHDPHYDRARLRAAAAPLDQAGLSAKALAASAARLGDDRAF
WARHLSALLAGATRACGEGWLVLDRDALAASDPWAAARALAWAAGAIAGSERAADPRPIAALLATPPAPGQVHALAGCLW
QSKPRGALVLCRECRGLDRRRWAVPPGESLLWDGRWLCRAGRGGLAVSALGAAGWQKLAPALAGDRQGLPPPIALPALPI
FYGESAEERADVFAVPSLGLWGYGYNPGSAFRGEMPFSVDITYHPFLPLVPLGLRVGAGGGL
>Mature_461_residues
APRGLVDANGVIDAEAFAARMAALGPFEPEPAIAVGVSGGVDSLTLLAFAARWAAERGGRCTAVCFDHGLRAASAGEARL
VETTARSLGAEAVVLRWAGPPPSSGVQEAARRARLDGLLGWCAGAGVLHLLLAHHRDDQAETVLQRLGRGSGADGLAAMA
ALSSRGEARILRPFLEVPRAAIEASARRIGLVWAEDPSNHDPHYDRARLRAAAAPLDQAGLSAKALAASAARLGDDRAFW
ARHLSALLAGATRACGEGWLVLDRDALAASDPWAAARALAWAAGAIAGSERAADPRPIAALLATPPAPGQVHALAGCLWQ
SKPRGALVLCRECRGLDRRRWAVPPGESLLWDGRWLCRAGRGGLAVSALGAAGWQKLAPALAGDRQGLPPPIALPALPIF
YGESAEERADVFAVPSLGLWGYGYNPGSAFRGEMPFSVDITYHPFLPLVPLGLRVGAGGGL

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=187, Percent_Identity=36.8983957219251, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 47962; Mature: 47831

Theoretical pI: Translated: 8.41; Mature: 8.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPRGLVDANGVIDAEAFAARMAALGPFEPEPAIAVGVSGGVDSLTLLAFAARWAAERGG
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCC
RCTAVCFDHGLRAASAGEARLVETTARSLGAEAVVLRWAGPPPSSGVQEAARRARLDGLL
CEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
GWCAGAGVLHLLLAHHRDDQAETVLQRLGRGSGADGLAAMAALSSRGEARILRPFLEVPR
HHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCH
AAIEASARRIGLVWAEDPSNHDPHYDRARLRAAAAPLDQAGLSAKALAASAARLGDDRAF
HHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCHHHHCCCHHHHHHHHHHCCCHHHH
WARHLSALLAGATRACGEGWLVLDRDALAASDPWAAARALAWAAGAIAGSERAADPRPIA
HHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEE
ALLATPPAPGQVHALAGCLWQSKPRGALVLCRECRGLDRRRWAVPPGESLLWDGRWLCRA
EEEECCCCCCHHHHHHHHHHCCCCCCCEEEEHHHCCCCCCCCCCCCCCCEEECCEEEEEC
GRGGLAVSALGAAGWQKLAPALAGDRQGLPPPIALPALPIFYGESAEERADVFAVPSLGL
CCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCHHCCCEEECCCCCC
WGYGYNPGSAFRGEMPFSVDITYHPFLPLVPLGLRVGAGGGL
CCCCCCCCCCCCCCCCEEEEEEECCCCCHHCCCEEECCCCCC
>Mature Secondary Structure 
APRGLVDANGVIDAEAFAARMAALGPFEPEPAIAVGVSGGVDSLTLLAFAARWAAERGG
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCC
RCTAVCFDHGLRAASAGEARLVETTARSLGAEAVVLRWAGPPPSSGVQEAARRARLDGLL
CEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
GWCAGAGVLHLLLAHHRDDQAETVLQRLGRGSGADGLAAMAALSSRGEARILRPFLEVPR
HHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCH
AAIEASARRIGLVWAEDPSNHDPHYDRARLRAAAAPLDQAGLSAKALAASAARLGDDRAF
HHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCHHHHCCCHHHHHHHHHHCCCHHHH
WARHLSALLAGATRACGEGWLVLDRDALAASDPWAAARALAWAAGAIAGSERAADPRPIA
HHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEE
ALLATPPAPGQVHALAGCLWQSKPRGALVLCRECRGLDRRRWAVPPGESLLWDGRWLCRA
EEEECCCCCCHHHHHHHHHHCCCCCCCEEEEHHHCCCCCCCCCCCCCCCEEECCEEEEEC
GRGGLAVSALGAAGWQKLAPALAGDRQGLPPPIALPALPIFYGESAEERADVFAVPSLGL
CCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCHHCCCEEECCCCCC
WGYGYNPGSAFRGEMPFSVDITYHPFLPLVPLGLRVGAGGGL
CCCCCCCCCCCCCCCCEEEEEEECCCCCHHCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA