Click here to switch to the map view.

The map label for this gene is murD

Identifier: 83592288

GI number: 83592288

Start: 1135925

End: 1137358

Strand: Reverse

Name: murD

Synonym: Rru_A0952

Alternate gene names: 83592288

Gene position: 1137358-1135925 (Counterclockwise)

Preceding gene: 83592289

Following gene: 83592287

Centisome position: 26.13

GC content: 70.08

Gene sequence:

>1434_bases
GTGGCGAAGCTAATCCCCCTTTACCAGTACGCCGGACAGGACCTGGGCATCATGGGCCTGGGCAAATCGGGAATGGCCAC
GGCGCGGGCGCTGGCGGGCACGGGAACGCGCGTCCACGCCTGGGACGACAGCCCGATCCTGCGTGACGCCGCCCGCGCCG
AGTCCGTTCCGCTGTGCGACCTCACCGACCAGACCAGCCGCACGTCCCCCTGGCCGGCGCTGCAGGGCGTGGTGTGGAGC
CCGGGCATTCCCCACACCTTCCCCCAGCCCCATCCGGTGGCGGTGATCGCCCACGAGCGCGGCGTGCCGCTGTTTTGCGA
CATCGACCTGCTGGCCCGCGCCCGCCAGGACTGCTTCTTCCTGGGCATCACCGGCACCAACGGCAAATCGACGACCACGG
CGCTGATCGGCCATATCCTGAAGGCGGCCCGCCACCCGGTGCAGGTCGGCGGCAATCTCGGCACCCCGGCGCTGTCGTTC
GAGCCCCTGCCCTTCCATGGCACCTATGTGCTGGAGCTGTCGTCCTATCAGCTCGAACTGGTGCCCTCGCTGTGCTGCGA
CGTGGCGGTGCTGCTCAACATCACCCCCGATCATCTGGCCCGCCATGGCGGCATGGACGGCTATATCGCCGCCAAGCGTC
AGATCTTCCGCTGCGGTCCGCGCCCGGGCGTCGCCGTCGTCTGCATCGACGACGAGCCGTCGCTGGCCATCCATGACGCG
CTGTGCCGCGAGAACGGTCGCAAGGTGGTGGCGGTCTCCACCCGCGCCCTGCCGCGCGGCGGCGTTGGCGCCGTCGACGG
CCAGTTGGTCGACGCCACCCAGGGCCGGCCGCGCGCCGTCGCCGATCTGACGACGATCGCCACCCTGCCCGGCGCCCACA
ACTGGCAAAACGCCGCCGCCGCCTATGCCGCCTGCCGCCAGCATGGGATCGACGCCAAGATCATCGTCGAGGCGATGGCC
AGCTTCCCCGGCCTGCCCCACCGCCAGGAACTGGTGGCCGAGATCGACGGCGTGCGCTTCATCAACGACAGCAAGGCGAC
CAATGCCGACGCCGCCGACAAGGCGCTGCGCTGCTATGACACGGTTTATTGGATCGCCGGCGGCCAGCCCAAGGAAGGCG
GCATCGTCAGCCTCGAGCCGCATTTCCACCGCATGCGCCAGGCCTATCTGATCGGTCAGGCCGCCCCGGCCTTTGAGCGC
ACCCTGAAGGGCAAGGTTCCCCTGTCCCAGGTCGGCACCCTGACCCGCGCCGTCACCGAAGCCGCCAAGGCCGCCTGGCG
CGACGCCATCCCCGGCGCCGTCGTTTTGCTGTCGCCGGCCTGCGCCTCGTGGGATCAGTTCTCCAGCTTCGAGCATCGCG
GCGACATCTTCCGCGAACTCGTCGCCGATCTCGCCCATACCCGGGCCGCCGCCAAGCCGGGGGGCCGCCGATGA

Upstream 100 bases:

>100_bases
GGCTGGGCCGAGCCCACGGTGGTGATCCGGTTCTGGATCATCGCCATGATCCTGGCCATCGTCGGCCTTTCCACCTTGAA
GCTGCGTTAGGAGAAAAGGG

Downstream 100 bases:

>100_bases
CCATGCGCGTCGTCACCGCCCCGGCCGAACCGCCCCAGGGGGCAAGCCATGTCGCCCCCTCGTTCACGCGGATGGACACC
AGCGTTCTTGGGCGCTGGTG

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MAKLIPLYQYAGQDLGIMGLGKSGMATARALAGTGTRVHAWDDSPILRDAARAESVPLCDLTDQTSRTSPWPALQGVVWS
PGIPHTFPQPHPVAVIAHERGVPLFCDIDLLARARQDCFFLGITGTNGKSTTTALIGHILKAARHPVQVGGNLGTPALSF
EPLPFHGTYVLELSSYQLELVPSLCCDVAVLLNITPDHLARHGGMDGYIAAKRQIFRCGPRPGVAVVCIDDEPSLAIHDA
LCRENGRKVVAVSTRALPRGGVGAVDGQLVDATQGRPRAVADLTTIATLPGAHNWQNAAAAYAACRQHGIDAKIIVEAMA
SFPGLPHRQELVAEIDGVRFINDSKATNADAADKALRCYDTVYWIAGGQPKEGGIVSLEPHFHRMRQAYLIGQAAPAFER
TLKGKVPLSQVGTLTRAVTEAAKAAWRDAIPGAVVLLSPACASWDQFSSFEHRGDIFRELVADLAHTRAAAKPGGRR

Sequences:

>Translated_477_residues
MAKLIPLYQYAGQDLGIMGLGKSGMATARALAGTGTRVHAWDDSPILRDAARAESVPLCDLTDQTSRTSPWPALQGVVWS
PGIPHTFPQPHPVAVIAHERGVPLFCDIDLLARARQDCFFLGITGTNGKSTTTALIGHILKAARHPVQVGGNLGTPALSF
EPLPFHGTYVLELSSYQLELVPSLCCDVAVLLNITPDHLARHGGMDGYIAAKRQIFRCGPRPGVAVVCIDDEPSLAIHDA
LCRENGRKVVAVSTRALPRGGVGAVDGQLVDATQGRPRAVADLTTIATLPGAHNWQNAAAAYAACRQHGIDAKIIVEAMA
SFPGLPHRQELVAEIDGVRFINDSKATNADAADKALRCYDTVYWIAGGQPKEGGIVSLEPHFHRMRQAYLIGQAAPAFER
TLKGKVPLSQVGTLTRAVTEAAKAAWRDAIPGAVVLLSPACASWDQFSSFEHRGDIFRELVADLAHTRAAAKPGGRR
>Mature_476_residues
AKLIPLYQYAGQDLGIMGLGKSGMATARALAGTGTRVHAWDDSPILRDAARAESVPLCDLTDQTSRTSPWPALQGVVWSP
GIPHTFPQPHPVAVIAHERGVPLFCDIDLLARARQDCFFLGITGTNGKSTTTALIGHILKAARHPVQVGGNLGTPALSFE
PLPFHGTYVLELSSYQLELVPSLCCDVAVLLNITPDHLARHGGMDGYIAAKRQIFRCGPRPGVAVVCIDDEPSLAIHDAL
CRENGRKVVAVSTRALPRGGVGAVDGQLVDATQGRPRAVADLTTIATLPGAHNWQNAAAAYAACRQHGIDAKIIVEAMAS
FPGLPHRQELVAEIDGVRFINDSKATNADAADKALRCYDTVYWIAGGQPKEGGIVSLEPHFHRMRQAYLIGQAAPAFERT
LKGKVPLSQVGTLTRAVTEAAKAAWRDAIPGAVVLLSPACASWDQFSSFEHRGDIFRELVADLAHTRAAAKPGGRR

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=470, Percent_Identity=34.0425531914894, Blast_Score=166, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_RHORT (Q2RVU2)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_426040.1
- ProteinModelPortal:   Q2RVU2
- SMR:   Q2RVU2
- STRING:   Q2RVU2
- GeneID:   3833625
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A0952
- NMPDR:   fig|1085.1.peg.3420
- eggNOG:   COG0771
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- PhylomeDB:   Q2RVU2
- ProtClustDB:   PRK01390
- BioCyc:   RRUB269796:RRU_A0952-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 50877; Mature: 50746

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKLIPLYQYAGQDLGIMGLGKSGMATARALAGTGTRVHAWDDSPILRDAARAESVPLCD
CCCEECHHHHCCCCCEEEEECCCCCHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCEEC
LTDQTSRTSPWPALQGVVWSPGIPHTFPQPHPVAVIAHERGVPLFCDIDLLARARQDCFF
CCCCCCCCCCCHHHCCEEECCCCCCCCCCCCCEEEEEECCCCCEEECHHHHHHCCCCEEE
LGITGTNGKSTTTALIGHILKAARHPVQVGGNLGTPALSFEPLPFHGTYVLELSSYQLEL
EEEECCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCEEEEEECCCCEEH
VPSLCCDVAVLLNITPDHLARHGGMDGYIAAKRQIFRCGPRPGVAVVCIDDEPSLAIHDA
HHHHHHHHHHEEECCHHHHHHHCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHH
LCRENGRKVVAVSTRALPRGGVGAVDGQLVDATQGRPRAVADLTTIATLPGAHNWQNAAA
HHHCCCCEEEEEEECCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHCCCCCCCCHHHHH
AYAACRQHGIDAKIIVEAMASFPGLPHRQELVAEIDGVRFINDSKATNADAADKALRCYD
HHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHHH
TVYWIAGGQPKEGGIVSLEPHFHRMRQAYLIGQAAPAFERTLKGKVPLSQVGTLTRAVTE
EEEEEECCCCCCCCEEEECHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHH
AAKAAWRDAIPGAVVLLSPACASWDQFSSFEHRGDIFRELVADLAHTRAAAKPGGRR
HHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AKLIPLYQYAGQDLGIMGLGKSGMATARALAGTGTRVHAWDDSPILRDAARAESVPLCD
CCEECHHHHCCCCCEEEEECCCCCHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCEEC
LTDQTSRTSPWPALQGVVWSPGIPHTFPQPHPVAVIAHERGVPLFCDIDLLARARQDCFF
CCCCCCCCCCCHHHCCEEECCCCCCCCCCCCCEEEEEECCCCCEEECHHHHHHCCCCEEE
LGITGTNGKSTTTALIGHILKAARHPVQVGGNLGTPALSFEPLPFHGTYVLELSSYQLEL
EEEECCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCEEEEEECCCCEEH
VPSLCCDVAVLLNITPDHLARHGGMDGYIAAKRQIFRCGPRPGVAVVCIDDEPSLAIHDA
HHHHHHHHHHEEECCHHHHHHHCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHH
LCRENGRKVVAVSTRALPRGGVGAVDGQLVDATQGRPRAVADLTTIATLPGAHNWQNAAA
HHHCCCCEEEEEEECCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHCCCCCCCCHHHHH
AYAACRQHGIDAKIIVEAMASFPGLPHRQELVAEIDGVRFINDSKATNADAADKALRCYD
HHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHHH
TVYWIAGGQPKEGGIVSLEPHFHRMRQAYLIGQAAPAFERTLKGKVPLSQVGTLTRAVTE
EEEEEECCCCCCCCEEEECHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHH
AAKAAWRDAIPGAVVLLSPACASWDQFSSFEHRGDIFRELVADLAHTRAAAKPGGRR
HHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA