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The map label for this gene is emrA [H]

Identifier: 83592195

GI number: 83592195

Start: 1023974

End: 1025182

Strand: Reverse

Name: emrA [H]

Synonym: Rru_A0859

Alternate gene names: 83592195

Gene position: 1025182-1023974 (Counterclockwise)

Preceding gene: 83592196

Following gene: 83592194

Centisome position: 23.55

GC content: 67.91

Gene sequence:

>1209_bases
ATGACCGAGCCCCCCTCCCTCCCCTCTTCCGATGACAGCGACCGGCGACGCCGGACCTTGTTGATGTCCGCCGCCGCCCT
GACGTTCATCGCCCTGGGCGCCGCCTATGGGATTTACTGGTGGACGACGGCCCGCTTCGACATGTCCACCGACAACGCCT
ATGTCGCGGGCAATATCATCCAGGTCTCGCCCCAGATCGCCGGCACGGTGACGGCGGTCCATGCCGACGATACGGATTTC
GTCGAAGCCGGACAGATGCTGGTCACCATCGACCCGACCGACGCCCGTCTGGCCCTTGATCAGGCGGAAGCGACTTTGGC
CGAAACGGTGCGCCAGGTTCGCGCCCTGTTCAGCCAGGATGGCGTTCTGGCGGCAACGGTCCTGGCCCAGGAAACCACCC
TGGCCCGGCTGCGCGAGGATTTCGCCCGCCGTCAAAGACTGGCCAACACCGGCGCCGTTTCGGCCGAAGAGGTCAAACAC
GCCCGTGACGCGGTGACGCAGGCCTCGGCGCACTTGGCCGGCGCCCGCAAGCAGCAGGAAGCCAACCGCGTGCTGATCGA
CATGACCGGCCTAACCAGCCATCCCCGCGTCGCCCGGGCCGCGGCCCAAGTGCGCGAAGCCTATGTGGCGTGGCGGCGCA
CCGTGGTTCCGGCGCCGGTGTCGGGTCAGGTCGCCCGCCGCGCCGTTCAGGTCGGCCAACGAACCACCCCCGGCACGCCC
TTGATGGCGATCATTCCGCTCGACGAGGTTTGGGTCGACGCGAATTTCAAGGAAGGCCAATTGGGAGCGATGCGCCTCGG
CCAACCCGTCCTGCTCACCGCCGACATCTATGGCGACGACATTGTCTATCGCGGCACGGTGGCCGGACTGGCGGCGGGAA
CCGGGGCGGCCTTTTCGCTGCTGCCGGCCCAGAACGCCACCGGCAACTGGATCAAGGTGGTCCAGCGCGTTCCGGTTCGC
ATCGCCCTTGATCCCCGGGATCTGGCCCAGCGTCCGCTGCGGGTCGGGCTTTCGATGGAGGCCCACGTCTCGATCAGGGA
TCAAAGCGGTCCGCAGATGGCGACGGGCCGGCAAAAGGACATCGTCGCCCGGACCGACGTCTTCGCCGACCTCGACCACG
ACGCCGAGGCGCTGATCAAGGACCTGATCGCCCGGAACATGCGGACGGAGCCGGACCTCGCGCCGGCGCCGATAACCGCC
GACCGGTGA

Upstream 100 bases:

>100_bases
GGACCCCGCTCCCCGCGCACCGCTCTAAGACCTTGATAGTTAAGCAAATCATCGTCGCACTCTGCTTCAAAGCGCTCTAA
CGATCCCAAGGACGCCCGCG

Downstream 100 bases:

>100_bases
GATCGGCCCCCAAGGGAGACGCCCCCATGCCCCCTCCGTCCTTTCCGCCCCTGACCGGCAACCGGCTGGTCCTCGCCACC
ATCGCCTTGTCGCTGGCCAC

Product: secretion protein HlyD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MTEPPSLPSSDDSDRRRRTLLMSAAALTFIALGAAYGIYWWTTARFDMSTDNAYVAGNIIQVSPQIAGTVTAVHADDTDF
VEAGQMLVTIDPTDARLALDQAEATLAETVRQVRALFSQDGVLAATVLAQETTLARLREDFARRQRLANTGAVSAEEVKH
ARDAVTQASAHLAGARKQQEANRVLIDMTGLTSHPRVARAAAQVREAYVAWRRTVVPAPVSGQVARRAVQVGQRTTPGTP
LMAIIPLDEVWVDANFKEGQLGAMRLGQPVLLTADIYGDDIVYRGTVAGLAAGTGAAFSLLPAQNATGNWIKVVQRVPVR
IALDPRDLAQRPLRVGLSMEAHVSIRDQSGPQMATGRQKDIVARTDVFADLDHDAEALIKDLIARNMRTEPDLAPAPITA
DR

Sequences:

>Translated_402_residues
MTEPPSLPSSDDSDRRRRTLLMSAAALTFIALGAAYGIYWWTTARFDMSTDNAYVAGNIIQVSPQIAGTVTAVHADDTDF
VEAGQMLVTIDPTDARLALDQAEATLAETVRQVRALFSQDGVLAATVLAQETTLARLREDFARRQRLANTGAVSAEEVKH
ARDAVTQASAHLAGARKQQEANRVLIDMTGLTSHPRVARAAAQVREAYVAWRRTVVPAPVSGQVARRAVQVGQRTTPGTP
LMAIIPLDEVWVDANFKEGQLGAMRLGQPVLLTADIYGDDIVYRGTVAGLAAGTGAAFSLLPAQNATGNWIKVVQRVPVR
IALDPRDLAQRPLRVGLSMEAHVSIRDQSGPQMATGRQKDIVARTDVFADLDHDAEALIKDLIARNMRTEPDLAPAPITA
DR
>Mature_401_residues
TEPPSLPSSDDSDRRRRTLLMSAAALTFIALGAAYGIYWWTTARFDMSTDNAYVAGNIIQVSPQIAGTVTAVHADDTDFV
EAGQMLVTIDPTDARLALDQAEATLAETVRQVRALFSQDGVLAATVLAQETTLARLREDFARRQRLANTGAVSAEEVKHA
RDAVTQASAHLAGARKQQEANRVLIDMTGLTSHPRVARAAAQVREAYVAWRRTVVPAPVSGQVARRAVQVGQRTTPGTPL
MAIIPLDEVWVDANFKEGQLGAMRLGQPVLLTADIYGDDIVYRGTVAGLAAGTGAAFSLLPAQNATGNWIKVVQRVPVRI
ALDPRDLAQRPLRVGLSMEAHVSIRDQSGPQMATGRQKDIVARTDVFADLDHDAEALIKDLIARNMRTEPDLAPAPITAD
R

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789041, Length=354, Percent_Identity=49.4350282485876, Blast_Score=338, Evalue=4e-94,
Organism=Escherichia coli, GI1788711, Length=346, Percent_Identity=43.3526011560694, Blast_Score=306, Evalue=1e-84,
Organism=Escherichia coli, GI1789637, Length=307, Percent_Identity=27.6872964169381, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1790519, Length=313, Percent_Identity=29.3929712460064, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI87081951, Length=292, Percent_Identity=25, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1790012, Length=246, Percent_Identity=24.7967479674797, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 43351; Mature: 43220

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPPSLPSSDDSDRRRRTLLMSAAALTFIALGAAYGIYWWTTARFDMSTDNAYVAGNII
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCEEEECCEE
QVSPQIAGTVTAVHADDTDFVEAGQMLVTIDPTDARLALDQAEATLAETVRQVRALFSQD
EECCCCCEEEEEEECCCCCHHCCCCEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHCCC
GVLAATVLAQETTLARLREDFARRQRLANTGAVSAEEVKHARDAVTQASAHLAGARKQQE
CCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
ANRVLIDMTGLTSHPRVARAAAQVREAYVAWRRTVVPAPVSGQVARRAVQVGQRTTPGTP
CCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCC
LMAIIPLDEVWVDANFKEGQLGAMRLGQPVLLTADIYGDDIVYRGTVAGLAAGTGAAFSL
EEEEEECHHEEEECCCCCCCCCHHHCCCCEEEEEEECCCCEEEECCHHHHHCCCCCEEEE
LPAQNATGNWIKVVQRVPVRIALDPRDLAQRPLRVGLSMEAHVSIRDQSGPQMATGRQKD
ECCCCCCCHHHHHHHHCCEEEEECHHHHHCCHHHHCCCEEEEEEEECCCCCCCCCCCCCC
IVARTDVFADLDHDAEALIKDLIARNMRTEPDLAPAPITADR
EEEHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TEPPSLPSSDDSDRRRRTLLMSAAALTFIALGAAYGIYWWTTARFDMSTDNAYVAGNII
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCEEEECCEE
QVSPQIAGTVTAVHADDTDFVEAGQMLVTIDPTDARLALDQAEATLAETVRQVRALFSQD
EECCCCCEEEEEEECCCCCHHCCCCEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHCCC
GVLAATVLAQETTLARLREDFARRQRLANTGAVSAEEVKHARDAVTQASAHLAGARKQQE
CCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
ANRVLIDMTGLTSHPRVARAAAQVREAYVAWRRTVVPAPVSGQVARRAVQVGQRTTPGTP
CCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCC
LMAIIPLDEVWVDANFKEGQLGAMRLGQPVLLTADIYGDDIVYRGTVAGLAAGTGAAFSL
EEEEEECHHEEEECCCCCCCCCHHHCCCCEEEEEEECCCCEEEECCHHHHHCCCCCEEEE
LPAQNATGNWIKVVQRVPVRIALDPRDLAQRPLRVGLSMEAHVSIRDQSGPQMATGRQKD
ECCCCCCCHHHHHHHHCCEEEEECHHHHHCCHHHHCCCEEEEEEEECCCCCCCCCCCCCC
IVARTDVFADLDHDAEALIKDLIARNMRTEPDLAPAPITADR
EEEHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1409590; 9205837; 9278503; 8203018 [H]