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The map label for this gene is emrB [H]

Identifier: 83592194

GI number: 83592194

Start: 1022400

End: 1023947

Strand: Reverse

Name: emrB [H]

Synonym: Rru_A0858

Alternate gene names: 83592194

Gene position: 1023947-1022400 (Counterclockwise)

Preceding gene: 83592195

Following gene: 83592193

Centisome position: 23.52

GC content: 61.69

Gene sequence:

>1548_bases
ATGCCCCCTCCGTCCTTTCCGCCCCTGACCGGCAACCGGCTGGTCCTCGCCACCATCGCCTTGTCGCTGGCCACTTTCAT
GAACGTGCTCGACACCACCATCGCCAATGTGGCCCTGACCACCATTGCCGGCGATCTTGGCGTCAGCGTCAGCCAGGGAA
CCTGGATCATCACCTCGTTTGGCGTCTCCAACGCCATCGCCGTGCCGCTGACCGGCTGGCTGGTCGGGCGGATCGGACAA
TTGCGGCTGTTCTTATCGGCGGTGGTGTTGTTCGTCGTGCTGTCGCTGTTGTGCGGGTTATCGACCAATCTGGAATCCCT
GCTGGCGTTCCGTTGCCTTCAGGGGCTGGTCGCCGGACCGATGGTGCCGCTGTCCCAGGCGCTGTTGCTGCAATGCTATC
CCAAGGCCAAGGCCGGGATGGCGATTGCCCTGTGGTCGATGACAACGACGGTGGCGCCGGTGATGGGGCCGGTTCTCGGC
GGCTGGCTGACCGACAACGTGTCCTGGCCGTGGATCTTCTACATCAATGGTCCAATCGGCCTGATCGCGGCGTGGCTCAC
CTTCGTCACCCTGAAAGGACGCGAGAGCGCCACCCGCACCCTGCCGATCGACACCATCGGCTTGGCTCTTCTGGTGCTGT
GGGTCGGCGCGACGCAGATCATGCTCGACAAGGGCAAGGAACTCGACTGGTTCAATTCCTCGACCATCATGGCGCTGGCG
ATCATCGCCGTCGTCACCTTCACGTTCTTCCTGATCTGGGAATTGACCCAAAAGCATCCGATCGTCGATCTGACCTTGTT
CAAGGGCCGCAACTTCACCACCGGAACCATCGCCATCACCCTGGGCTATGCGGTTTTCTTCGGAAATCTGGTGGTCATCC
CGATGTGGTTGCAAAGCGTCATGGGGTATACGGCCACCTGGGCCGGGCTGGTCACCGCGCCGATCGGATTTTTCTCGCTT
CTCTTGTCGCCTTTGATCGGCCGCACCATTCACAAGGTCGATCCCCGACTGTTCGCCAGCTTCGCCTTCTTCATTTTCGC
CGTCTGCAGCTTCTGGCGCTCAACCTTCAGCCCCGATGTCGCCCTGTGGGACATCGCCGCCAGCCATTTGCTGCAAGGCT
TCGCCATGGCGACGTTTTTCATCGCCCTGACCACGGTGATCCTGTCGGGGCTGGATCAGCAGCGCATTCCCGCCGCCTCG
GGCCTTTACAACTTCATGCGGATCATGGCCGGCTCGTTCGCCGCCTCGGTGTGGACCACCCTGTGGGAGAATGGCGCGAC
CCGGCACCACGCCTATCTAACCGAAAGCGTCACCGCCTATTCCGCGCCGACCGCCCATTTTGAAGCCGGCCTGGGAGCCC
TTGGACTGACCACCGGCCAAATCTACGGCATCATCAACGCGGAAATCGGCCGCCAGTCCCTGATTTTGTCGATCACCGAC
CTGTTCTGGCTGTCGGGTCTGTTGTTCCTGGTCCTGATCGGCCTGATCTGGCTGTCCCATCCGGTTCGCCGGGAAACACG
CCCCGCCAAGGACGATCGAAACCGCTAA

Upstream 100 bases:

>100_bases
TCAAGGACCTGATCGCCCGGAACATGCGGACGGAGCCGGACCTCGCGCCGGCGCCGATAACCGCCGACCGGTGAGATCGG
CCCCCAAGGGAGACGCCCCC

Downstream 100 bases:

>100_bases
AGACTAGGCGCGCAACCGGAGGAAAACCACCCAAGGAATAAGAGCGTTTAAGGGGCAGATCCTGAAAAGCCAATATTTTC
TCAATGGTTTACTTCGTTCT

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 515; Mature: 514

Protein sequence:

>515_residues
MPPPSFPPLTGNRLVLATIALSLATFMNVLDTTIANVALTTIAGDLGVSVSQGTWIITSFGVSNAIAVPLTGWLVGRIGQ
LRLFLSAVVLFVVLSLLCGLSTNLESLLAFRCLQGLVAGPMVPLSQALLLQCYPKAKAGMAIALWSMTTTVAPVMGPVLG
GWLTDNVSWPWIFYINGPIGLIAAWLTFVTLKGRESATRTLPIDTIGLALLVLWVGATQIMLDKGKELDWFNSSTIMALA
IIAVVTFTFFLIWELTQKHPIVDLTLFKGRNFTTGTIAITLGYAVFFGNLVVIPMWLQSVMGYTATWAGLVTAPIGFFSL
LLSPLIGRTIHKVDPRLFASFAFFIFAVCSFWRSTFSPDVALWDIAASHLLQGFAMATFFIALTTVILSGLDQQRIPAAS
GLYNFMRIMAGSFAASVWTTLWENGATRHHAYLTESVTAYSAPTAHFEAGLGALGLTTGQIYGIINAEIGRQSLILSITD
LFWLSGLLFLVLIGLIWLSHPVRRETRPAKDDRNR

Sequences:

>Translated_515_residues
MPPPSFPPLTGNRLVLATIALSLATFMNVLDTTIANVALTTIAGDLGVSVSQGTWIITSFGVSNAIAVPLTGWLVGRIGQ
LRLFLSAVVLFVVLSLLCGLSTNLESLLAFRCLQGLVAGPMVPLSQALLLQCYPKAKAGMAIALWSMTTTVAPVMGPVLG
GWLTDNVSWPWIFYINGPIGLIAAWLTFVTLKGRESATRTLPIDTIGLALLVLWVGATQIMLDKGKELDWFNSSTIMALA
IIAVVTFTFFLIWELTQKHPIVDLTLFKGRNFTTGTIAITLGYAVFFGNLVVIPMWLQSVMGYTATWAGLVTAPIGFFSL
LLSPLIGRTIHKVDPRLFASFAFFIFAVCSFWRSTFSPDVALWDIAASHLLQGFAMATFFIALTTVILSGLDQQRIPAAS
GLYNFMRIMAGSFAASVWTTLWENGATRHHAYLTESVTAYSAPTAHFEAGLGALGLTTGQIYGIINAEIGRQSLILSITD
LFWLSGLLFLVLIGLIWLSHPVRRETRPAKDDRNR
>Mature_514_residues
PPPSFPPLTGNRLVLATIALSLATFMNVLDTTIANVALTTIAGDLGVSVSQGTWIITSFGVSNAIAVPLTGWLVGRIGQL
RLFLSAVVLFVVLSLLCGLSTNLESLLAFRCLQGLVAGPMVPLSQALLLQCYPKAKAGMAIALWSMTTTVAPVMGPVLGG
WLTDNVSWPWIFYINGPIGLIAAWLTFVTLKGRESATRTLPIDTIGLALLVLWVGATQIMLDKGKELDWFNSSTIMALAI
IAVVTFTFFLIWELTQKHPIVDLTLFKGRNFTTGTIAITLGYAVFFGNLVVIPMWLQSVMGYTATWAGLVTAPIGFFSLL
LSPLIGRTIHKVDPRLFASFAFFIFAVCSFWRSTFSPDVALWDIAASHLLQGFAMATFFIALTTVILSGLDQQRIPAASG
LYNFMRIMAGSFAASVWTTLWENGATRHHAYLTESVTAYSAPTAHFEAGLGALGLTTGQIYGIINAEIGRQSLILSITDL
FWLSGLLFLVLIGLIWLSHPVRRETRPAKDDRNR

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=495, Percent_Identity=53.3333333333333, Blast_Score=523, Evalue=1e-150,
Organism=Escherichia coli, GI1788710, Length=498, Percent_Identity=49.3975903614458, Blast_Score=464, Evalue=1e-132,
Organism=Escherichia coli, GI1790195, Length=389, Percent_Identity=31.6195372750643, Blast_Score=148, Evalue=7e-37,
Organism=Escherichia coli, GI87081983, Length=415, Percent_Identity=26.7469879518072, Blast_Score=119, Evalue=6e-28,
Organism=Escherichia coli, GI1788392, Length=312, Percent_Identity=31.4102564102564, Blast_Score=115, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6322958, Length=197, Percent_Identity=27.4111675126904, Blast_Score=74, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6321663, Length=153, Percent_Identity=30.718954248366, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6325455, Length=149, Percent_Identity=28.1879194630872, Blast_Score=67, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55871; Mature: 55739

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPPSFPPLTGNRLVLATIALSLATFMNVLDTTIANVALTTIAGDLGVSVSQGTWIITSF
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCEEEEEC
GVSNAIAVPLTGWLVGRIGQLRLFLSAVVLFVVLSLLCGLSTNLESLLAFRCLQGLVAGP
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
MVPLSQALLLQCYPKAKAGMAIALWSMTTTVAPVMGPVLGGWLTDNVSWPWIFYINGPIG
CHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHCCHHCCCCCCCEEEEECCCHH
LIAAWLTFVTLKGRESATRTLPIDTIGLALLVLWVGATQIMLDKGKELDWFNSSTIMALA
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHH
IIAVVTFTFFLIWELTQKHPIVDLTLFKGRNFTTGTIAITLGYAVFFGNLVVIPMWLQSV
HHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MGYTATWAGLVTAPIGFFSLLLSPLIGRTIHKVDPRLFASFAFFIFAVCSFWRSTFSPDV
HCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCH
ALWDIAASHLLQGFAMATFFIALTTVILSGLDQQRIPAASGLYNFMRIMAGSFAASVWTT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHH
LWENGATRHHAYLTESVTAYSAPTAHFEAGLGALGLTTGQIYGIINAEIGRQSLILSITD
HHHCCCCCHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHH
LFWLSGLLFLVLIGLIWLSHPVRRETRPAKDDRNR
HHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCC
>Mature Secondary Structure 
PPPSFPPLTGNRLVLATIALSLATFMNVLDTTIANVALTTIAGDLGVSVSQGTWIITSF
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCEEEEEC
GVSNAIAVPLTGWLVGRIGQLRLFLSAVVLFVVLSLLCGLSTNLESLLAFRCLQGLVAGP
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
MVPLSQALLLQCYPKAKAGMAIALWSMTTTVAPVMGPVLGGWLTDNVSWPWIFYINGPIG
CHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHCCHHCCCCCCCEEEEECCCHH
LIAAWLTFVTLKGRESATRTLPIDTIGLALLVLWVGATQIMLDKGKELDWFNSSTIMALA
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHH
IIAVVTFTFFLIWELTQKHPIVDLTLFKGRNFTTGTIAITLGYAVFFGNLVVIPMWLQSV
HHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MGYTATWAGLVTAPIGFFSLLLSPLIGRTIHKVDPRLFASFAFFIFAVCSFWRSTFSPDV
HCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCH
ALWDIAASHLLQGFAMATFFIALTTVILSGLDQQRIPAASGLYNFMRIMAGSFAASVWTT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHH
LWENGATRHHAYLTESVTAYSAPTAHFEAGLGALGLTTGQIYGIINAEIGRQSLILSITD
HHHCCCCCHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHH
LFWLSGLLFLVLIGLIWLSHPVRRETRPAKDDRNR
HHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]