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The map label for this gene is gcvA [H]

Identifier: 83592124

GI number: 83592124

Start: 937314

End: 938249

Strand: Direct

Name: gcvA [H]

Synonym: Rru_A0785

Alternate gene names: 83592124

Gene position: 937314-938249 (Clockwise)

Preceding gene: 83592123

Following gene: 83592127

Centisome position: 21.53

GC content: 68.59

Gene sequence:

>936_bases
ATGATGCCGCGCGACGATACTCTGCCGACCATCCGGCGCCGTCTGCCGCCACTGACCGCCTTGCGCACCTTCGAGGCGGT
CGCCCGTCACCTGAGCGTGACCAATGCCGCCGACGAATTATCGGTGACGCCGGCGGCGGTCAGCCAGCAGATCCGCCAGC
TTGAAGATACGCTGGGAACCGCCCTGCTGCGCCGCCATGGCCGGTCGCTGGCGTTGACCGATGCCGGGCGCGCTTTGCAG
CCGGGCTTGAGCGAGGCTTTCGATCGGCTGGTGCAGGCGATCACCGAGATGCGGCCGGCCGCCGATGCCCGGCCGGTGGT
GCGGGTGGCGGTCACGCCGTCCTTTGCCGAGAAATGGCTGGTGCCGCGCCTGGGTCTGTTCATCCGCGCCAATGAAGGGA
TCGACGTTCATGTCATGGCCGGCATGGAGCTGATGGATTTCGCCCGCGAGCCGGTCGATCTGGCGGTGCGCTTCGGCGCC
GGGCGCTATCCCGGTCTGACTGTGGAACTGCTGCGCGAGGAGGAAGTCTTTCCCGTCTGCGCCCCGGCGCTGTTAAGCGG
CGCCCAGCCGATCCGCAAGGTCGTCGATCTGCGCCACCATGTGCTGATCCATGACGACAATCCGGCCGAACGCGATGTCT
GCCCGGATTGGACGATGTGGCTGACGGCGGCGGGATTTGCCGATCTGCCGGCGGCCAATGGCCCGCGCTTCAACCAGTCG
TCGATGGTCCTCGAAGCCGCGGCCCAGGGCCTGGGCGTCGCCCTGGCCAAGGGGTTCCTGGCGCGCGCCGATCTGGAAAG
CGGCCGCCTCGCCCGACCTTTCGCCGAAAACCACGCGCTGCGCTTCGCCTATTGGGTGGTCTCCACCCCCCAGGCCCTGG
TGCGCCCCGAGGTCCGGCTGTTCCGCGACTGGCTGCTCGGCGAAGCCGCCGAATGA

Upstream 100 bases:

>100_bases
GAGGGGGGATACCCGCAAGGGTTTTTGTATACTAAATCTGTAGATATTGTTATTTTTAAGGGAGAGCGAAAGGCCTTCAT
CCTGGGCCGAGGGGAGACGA

Downstream 100 bases:

>100_bases
TCCGCGCCGAGGCCTAGACCGGCGTCCTTGAAATCTGGTTCAGGCACGCCGGTCTAGGATCTTGATAGGGGAGCAAATCG
TCGTCGCACTCTGGCTCAGA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MMPRDDTLPTIRRRLPPLTALRTFEAVARHLSVTNAADELSVTPAAVSQQIRQLEDTLGTALLRRHGRSLALTDAGRALQ
PGLSEAFDRLVQAITEMRPAADARPVVRVAVTPSFAEKWLVPRLGLFIRANEGIDVHVMAGMELMDFAREPVDLAVRFGA
GRYPGLTVELLREEEVFPVCAPALLSGAQPIRKVVDLRHHVLIHDDNPAERDVCPDWTMWLTAAGFADLPAANGPRFNQS
SMVLEAAAQGLGVALAKGFLARADLESGRLARPFAENHALRFAYWVVSTPQALVRPEVRLFRDWLLGEAAE

Sequences:

>Translated_311_residues
MMPRDDTLPTIRRRLPPLTALRTFEAVARHLSVTNAADELSVTPAAVSQQIRQLEDTLGTALLRRHGRSLALTDAGRALQ
PGLSEAFDRLVQAITEMRPAADARPVVRVAVTPSFAEKWLVPRLGLFIRANEGIDVHVMAGMELMDFAREPVDLAVRFGA
GRYPGLTVELLREEEVFPVCAPALLSGAQPIRKVVDLRHHVLIHDDNPAERDVCPDWTMWLTAAGFADLPAANGPRFNQS
SMVLEAAAQGLGVALAKGFLARADLESGRLARPFAENHALRFAYWVVSTPQALVRPEVRLFRDWLLGEAAE
>Mature_311_residues
MMPRDDTLPTIRRRLPPLTALRTFEAVARHLSVTNAADELSVTPAAVSQQIRQLEDTLGTALLRRHGRSLALTDAGRALQ
PGLSEAFDRLVQAITEMRPAADARPVVRVAVTPSFAEKWLVPRLGLFIRANEGIDVHVMAGMELMDFAREPVDLAVRFGA
GRYPGLTVELLREEEVFPVCAPALLSGAQPIRKVVDLRHHVLIHDDNPAERDVCPDWTMWLTAAGFADLPAANGPRFNQS
SMVLEAAAQGLGVALAKGFLARADLESGRLARPFAENHALRFAYWVVSTPQALVRPEVRLFRDWLLGEAAE

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=300, Percent_Identity=42.6666666666667, Blast_Score=225, Evalue=3e-60,
Organism=Escherichia coli, GI1788706, Length=303, Percent_Identity=33.3333333333333, Blast_Score=154, Evalue=7e-39,
Organism=Escherichia coli, GI1786448, Length=296, Percent_Identity=34.1216216216216, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI157672245, Length=150, Percent_Identity=35.3333333333333, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI145693193, Length=307, Percent_Identity=25.4071661237785, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789440, Length=262, Percent_Identity=25.9541984732824, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789639, Length=145, Percent_Identity=28.2758620689655, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34182; Mature: 34182

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMPRDDTLPTIRRRLPPLTALRTFEAVARHLSVTNAADELSVTPAAVSQQIRQLEDTLGT
CCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHH
ALLRRHGRSLALTDAGRALQPGLSEAFDRLVQAITEMRPAADARPVVRVAVTPSFAEKWL
HHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHH
VPRLGLFIRANEGIDVHVMAGMELMDFAREPVDLAVRFGAGRYPGLTVELLREEEVFPVC
HHHHCEEEEECCCCEEEEECCHHHHHHCCCCHHEEEEECCCCCCCCCCEECCCCCCCCHH
APALLSGAQPIRKVVDLRHHVLIHDDNPAERDVCPDWTMWLTAAGFADLPAANGPRFNQS
HHHHHCCHHHHHHHHHHHHEEEEECCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHH
SMVLEAAAQGLGVALAKGFLARADLESGRLARPFAENHALRFAYWVVSTPQALVRPEVRL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCEEEEEEEECCCHHHHHHHHHH
FRDWLLGEAAE
HHHHHHCCCCC
>Mature Secondary Structure
MMPRDDTLPTIRRRLPPLTALRTFEAVARHLSVTNAADELSVTPAAVSQQIRQLEDTLGT
CCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHH
ALLRRHGRSLALTDAGRALQPGLSEAFDRLVQAITEMRPAADARPVVRVAVTPSFAEKWL
HHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHH
VPRLGLFIRANEGIDVHVMAGMELMDFAREPVDLAVRFGAGRYPGLTVELLREEEVFPVC
HHHHCEEEEECCCCEEEEECCHHHHHHCCCCHHEEEEECCCCCCCCCCEECCCCCCCCHH
APALLSGAQPIRKVVDLRHHVLIHDDNPAERDVCPDWTMWLTAAGFADLPAANGPRFNQS
HHHHHCCHHHHHHHHHHHHEEEEECCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHH
SMVLEAAAQGLGVALAKGFLARADLESGRLARPFAENHALRFAYWVVSTPQALVRPEVRL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCEEEEEEEECCCHHHHHHHHHH
FRDWLLGEAAE
HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]