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The map label for this gene is mdeA [H]

Identifier: 83592123

GI number: 83592123

Start: 935651

End: 936979

Strand: Direct

Name: mdeA [H]

Synonym: Rru_A0784

Alternate gene names: 83592123

Gene position: 935651-936979 (Clockwise)

Preceding gene: 83592122

Following gene: 83592124

Centisome position: 21.5

GC content: 64.86

Gene sequence:

>1329_bases
ATGATCGACATGACCGCTCAGTCCACTATCCGGCCTTTGACCGGCCTCAAGAATCCCGAAACCCTGGCGTTGCACGGGGG
CACCTATCGGGCTGATCCGACCACCGGGTCGGTGGCCGTGCCGATCTATCAAACCACGTCCTATCAGTTCGAAAACACCG
GGCAGGCGGCGCGGCTTTTCGCCTTGCAGGAATTGGGCAATATCTATAGCCGGCTGACCAATCCGACCACCGACGCCTTT
GAACAGCGCATCGCCGCCATCGAAGGCGGGGTGGCGGCCCTGGCGGTTTCCTCGGGGCAGGCGGCTTCGACCTTCGCCGT
GCTCAACCTCGCCCAGGCCGGCGACAACATCGTCTCCTCGACCGATCTCTACGGCGGCACCTGGGCGCTGTTTGCCAGCA
CCTTCAAGCAGTTCGGCATCGAGGTCCGCTTCGTCGATCCGACCGATCCCGAGAATTTCCGCAAGGCGACCGACGAGCGC
ACCCGGGCCTATTACGCCGAGACCCTGCCCAACCCCAAGCTCAATGTCTTCCCGATCCGCGAGGTCGCCGATATCGGCCG
CTCGCTGGGCGTGCCGCTGATCGTTGATAACACGGCCGCGCCGCTGATCGCCCGGCCCTTCGACCATGGCGCGGCGGTGG
TTGTTTATTCGACGACCAAATACATCGGCGGCCATGGCACCACCATCGGCGGCGTCATCATCGATAGCGGCACCTTCCCC
TGGGAGGATTTCCCCGACCGCTTCCCGACCCTGACCGAGCCCGATGAAAGCTATCACGGCGCGGTATGGACCGAGGCGAC
CAAGCCGCTGGGGCCGGTGGCCTATATCATCCGCGCCCGGGTCAAGCTGCTGCGCGACGTGGGCGCGGCGATCTCGCCGC
TGGCGGTCTTTCAGTTGATCCAGGGTCTGGAAACCCTGCCGCTGCGCATCCGCCAGCACAACGAGAACGCCCTGAAGGTG
GCGACCTTCCTCAAGGATCATCCCAAGATCGCCCGGGTCATCTATCCCGGCCTGCAGACCGGCGAGCTTCGCCGTCGGGC
CGACGCCTATCTGACCGGCGGCTACGGCGGTTTGGTCGGCTTTGAACTCCATGGCGGGGCCGAGGCCGGGCGGGCCTTCA
TCGAAAGCCTGCGCCTGTTCTACCACGTCGCCAATATCGGCGATGCCCGCTCGCTGGCCATCCACCCGGCGACCACCACC
CATTCCCAGTTGTCCGAGGAGGATCAACTGCTGACCGGGGTCACCCCGGGCTATGTGCGGCTGTCGATCGGCATCGAACA
TCCCGCCGACATCATCGAAGACCTGTCCCAGGCCCTCGACAAGGCCTGA

Upstream 100 bases:

>100_bases
TAAACACAAATTCTAACTGTAGTATTTGATCTTATCATCAGCCGTCTCGTGTTAATTGTTGGGTCAAGCAATCGGGCGTT
CCGATTCCCCATAGCGACGG

Downstream 100 bases:

>100_bases
GCTCGCGCGGCGTTCGGGGGGGAGCGGGGGCGGGCGGCCCTTGCTTTCCCCGGCGCCGGCCGGTTCGCCGCTCTTTTGGC
ACGAATTCATAAAGTTTGAT

Product: O-acetylhomoserine/O-acetylserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MIDMTAQSTIRPLTGLKNPETLALHGGTYRADPTTGSVAVPIYQTTSYQFENTGQAARLFALQELGNIYSRLTNPTTDAF
EQRIAAIEGGVAALAVSSGQAASTFAVLNLAQAGDNIVSSTDLYGGTWALFASTFKQFGIEVRFVDPTDPENFRKATDER
TRAYYAETLPNPKLNVFPIREVADIGRSLGVPLIVDNTAAPLIARPFDHGAAVVVYSTTKYIGGHGTTIGGVIIDSGTFP
WEDFPDRFPTLTEPDESYHGAVWTEATKPLGPVAYIIRARVKLLRDVGAAISPLAVFQLIQGLETLPLRIRQHNENALKV
ATFLKDHPKIARVIYPGLQTGELRRRADAYLTGGYGGLVGFELHGGAEAGRAFIESLRLFYHVANIGDARSLAIHPATTT
HSQLSEEDQLLTGVTPGYVRLSIGIEHPADIIEDLSQALDKA

Sequences:

>Translated_442_residues
MIDMTAQSTIRPLTGLKNPETLALHGGTYRADPTTGSVAVPIYQTTSYQFENTGQAARLFALQELGNIYSRLTNPTTDAF
EQRIAAIEGGVAALAVSSGQAASTFAVLNLAQAGDNIVSSTDLYGGTWALFASTFKQFGIEVRFVDPTDPENFRKATDER
TRAYYAETLPNPKLNVFPIREVADIGRSLGVPLIVDNTAAPLIARPFDHGAAVVVYSTTKYIGGHGTTIGGVIIDSGTFP
WEDFPDRFPTLTEPDESYHGAVWTEATKPLGPVAYIIRARVKLLRDVGAAISPLAVFQLIQGLETLPLRIRQHNENALKV
ATFLKDHPKIARVIYPGLQTGELRRRADAYLTGGYGGLVGFELHGGAEAGRAFIESLRLFYHVANIGDARSLAIHPATTT
HSQLSEEDQLLTGVTPGYVRLSIGIEHPADIIEDLSQALDKA
>Mature_442_residues
MIDMTAQSTIRPLTGLKNPETLALHGGTYRADPTTGSVAVPIYQTTSYQFENTGQAARLFALQELGNIYSRLTNPTTDAF
EQRIAAIEGGVAALAVSSGQAASTFAVLNLAQAGDNIVSSTDLYGGTWALFASTFKQFGIEVRFVDPTDPENFRKATDER
TRAYYAETLPNPKLNVFPIREVADIGRSLGVPLIVDNTAAPLIARPFDHGAAVVVYSTTKYIGGHGTTIGGVIIDSGTFP
WEDFPDRFPTLTEPDESYHGAVWTEATKPLGPVAYIIRARVKLLRDVGAAISPLAVFQLIQGLETLPLRIRQHNENALKV
ATFLKDHPKIARVIYPGLQTGELRRRADAYLTGGYGGLVGFELHGGAEAGRAFIESLRLFYHVANIGDARSLAIHPATTT
HSQLSEEDQLLTGVTPGYVRLSIGIEHPADIIEDLSQALDKA

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=428, Percent_Identity=31.7757009345794, Blast_Score=179, Evalue=5e-45,
Organism=Homo sapiens, GI25453487, Length=426, Percent_Identity=28.4037558685446, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI299473759, Length=428, Percent_Identity=28.2710280373832, Blast_Score=136, Evalue=4e-32,
Organism=Escherichia coli, GI1790375, Length=427, Percent_Identity=27.8688524590164, Blast_Score=169, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17538055, Length=427, Percent_Identity=33.2552693208431, Blast_Score=192, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI17560056, Length=426, Percent_Identity=33.3333333333333, Blast_Score=188, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI71980708, Length=421, Percent_Identity=31.353919239905, Blast_Score=187, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6323333, Length=434, Percent_Identity=47.2350230414747, Blast_Score=358, Evalue=1e-99,
Organism=Saccharomyces cerevisiae, GI6319307, Length=434, Percent_Identity=32.4884792626728, Blast_Score=174, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6321904, Length=435, Percent_Identity=27.3563218390805, Blast_Score=103, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6321170, Length=309, Percent_Identity=24.2718446601942, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI19922554, Length=429, Percent_Identity=31.2354312354312, Blast_Score=188, Evalue=7e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 47747; Mature: 47747

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDMTAQSTIRPLTGLKNPETLALHGGTYRADPTTGSVAVPIYQTTSYQFENTGQAARLF
CCCCCCHHHHHHHHCCCCCCEEEEECCEEECCCCCCCEEEEEEECCCEEECCCCHHHHHH
ALQELGNIYSRLTNPTTDAFEQRIAAIEGGVAALAVSSGQAASTFAVLNLAQAGDNIVSS
HHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCC
TDLYGGTWALFASTFKQFGIEVRFVDPTDPENFRKATDERTRAYYAETLPNPKLNVFPIR
CCCCCCHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECH
EVADIGRSLGVPLIVDNTAAPLIARPFDHGAAVVVYSTTKYIGGHGTTIGGVIIDSGTFP
HHHHHHHHCCCCEEECCCCCCCEECCCCCCCEEEEEEEEEEECCCCCEECEEEEECCCCC
WEDFPDRFPTLTEPDESYHGAVWTEATKPLGPVAYIIRARVKLLRDVGAAISPLAVFQLI
CCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGLETLPLRIRQHNENALKVATFLKDHPKIARVIYPGLQTGELRRRADAYLTGGYGGLVG
HCHHHCCHHEEECCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHCCEEECCCCCEEE
FELHGGAEAGRAFIESLRLFYHVANIGDARSLAIHPATTTHSQLSEEDQLLTGVTPGYVR
EEECCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHCCCHHEEECCCCCEEE
LSIGIEHPADIIEDLSQALDKA
EEECCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIDMTAQSTIRPLTGLKNPETLALHGGTYRADPTTGSVAVPIYQTTSYQFENTGQAARLF
CCCCCCHHHHHHHHCCCCCCEEEEECCEEECCCCCCCEEEEEEECCCEEECCCCHHHHHH
ALQELGNIYSRLTNPTTDAFEQRIAAIEGGVAALAVSSGQAASTFAVLNLAQAGDNIVSS
HHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCC
TDLYGGTWALFASTFKQFGIEVRFVDPTDPENFRKATDERTRAYYAETLPNPKLNVFPIR
CCCCCCHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECH
EVADIGRSLGVPLIVDNTAAPLIARPFDHGAAVVVYSTTKYIGGHGTTIGGVIIDSGTFP
HHHHHHHHCCCCEEECCCCCCCEECCCCCCCEEEEEEEEEEECCCCCEECEEEEECCCCC
WEDFPDRFPTLTEPDESYHGAVWTEATKPLGPVAYIIRARVKLLRDVGAAISPLAVFQLI
CCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGLETLPLRIRQHNENALKVATFLKDHPKIARVIYPGLQTGELRRRADAYLTGGYGGLVG
HCHHHCCHHEEECCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHCCEEECCCCCEEE
FELHGGAEAGRAFIESLRLFYHVANIGDARSLAIHPATTTHSQLSEEDQLLTGVTPGYVR
EEECCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHCCCHHEEECCCCCEEE
LSIGIEHPADIIEDLSQALDKA
EEECCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]