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The map label for this gene is ispG

Identifier: 83592086

GI number: 83592086

Start: 875140

End: 876294

Strand: Direct

Name: ispG

Synonym: Rru_A0747

Alternate gene names: 83592086

Gene position: 875140-876294 (Clockwise)

Preceding gene: 83592085

Following gene: 83592087

Centisome position: 20.11

GC content: 65.89

Gene sequence:

>1155_bases
ATGTCCGTCCGTACCTATCGCGACATCAGCCGCCGCGTTTCCCGCAAGATCCGTGTCGGATCGGTCTTTGTTGGCGGCGA
TGCGCCGGTCAGCGTGCAGACCATGACCAACACGCTGACCACCGATGTGGCGGCGACGGTCGCCCAGATCCGCCGGGCTC
AGGAGGCGGGGGCCGATATCGTGCGCGTTTCGTGCCCCGACGAGGAAAGCACGGCGGCGCTGAAGGCGATCATCTCGCAG
GTCGAGGTGCCGATCGTCGCCGACATCCATTTCCACTATAAGCGCGCCATCGAGGCCGCCGAGGCCGGCGCCGCCTGCCT
GCGCATCAATCCGGGCAATATCGGCAGCGCCGAGCGGGTGCGCGAAGTGGTGCGCGCCGCCAAGGACCACGGCTGTTCCA
TGCGCATCGGCGTCAATGCCGGATCGCTGGAAAAGGAGCTTCTGGAGAAATACGGCGAGCCTTGCCCCGAGGCGATGGTC
GAAAGCGCGCTGAGCCACGCCCGGATCCTGGAAGACAACGACTTCACCGAGTTCAAGATCAGCGTCAAGGCCAGCGACGC
CTTCCTGGCGGTCGCCGCCTATAAGGCGCTGGCCGAGGCCTGCGACTATCCGTTGCACCTGGGGATCACCGAGGCCGGCG
GTCTGCGCGGCGGCACGGTGAAATCGGCGATCGGCATCGGCAGCATGCTGTGGGCGGGGATCGGCGATACCATCCGCGTC
TCGCTTTCGGCCCAGCCCGAGGAAGAGGTCAAGGTCGGCTTCGAGATCCTGAAGTCGCTCAATCTGCGCCATCGCGGCGT
GCGCGTCGTGTCGTGCCCGTCCTGCGCCCGCCAGGGCTTCGACGTGATCAAGACGGTCGAGGTTCTGGAAAGCCGCCTGT
CGCACATCCAGACGCCGATAACCCTGTCGATCATCGGCTGCGTGGTCAATGGCCCGGGCGAGGCGCGGGAAACCGACATC
GGCCTGACCGGCGGCGGCAATCTGGCCAAGGAGGGCGCCCTCAACAAGGTCTATGTGTCGGGCATCCCCGATCACACCAT
CAACAACGAGGCGATGGTCGATCATCTGGTCGCCCTGGTCGAGAAAAAGGCCGCCGAGATCGAAGCCCTCGAGGCCGCCC
GCAAGGCCAAGGAACCGGCGACCGCCGCCGAATAG

Upstream 100 bases:

>100_bases
AAGGGCGGTGCGGCGACCGTCCAAGGACGGATCGCCCGGGGCGGGTGCCTGACGGCCGCCGCCGCCCCCCAACCGTGAGC
CGGTCACCGAAGGGATTTCC

Downstream 100 bases:

>100_bases
GACCGGACCCCGGCCGCTCATCCGGCCGGGGCCGCCTTTGCGTTCCTAGACAGCGGCCCCTTGGGGATGGTATCGCAGAG
CGGCCAACCGGGCCTTCCTG

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MSVRTYRDISRRVSRKIRVGSVFVGGDAPVSVQTMTNTLTTDVAATVAQIRRAQEAGADIVRVSCPDEESTAALKAIISQ
VEVPIVADIHFHYKRAIEAAEAGAACLRINPGNIGSAERVREVVRAAKDHGCSMRIGVNAGSLEKELLEKYGEPCPEAMV
ESALSHARILEDNDFTEFKISVKASDAFLAVAAYKALAEACDYPLHLGITEAGGLRGGTVKSAIGIGSMLWAGIGDTIRV
SLSAQPEEEVKVGFEILKSLNLRHRGVRVVSCPSCARQGFDVIKTVEVLESRLSHIQTPITLSIIGCVVNGPGEARETDI
GLTGGGNLAKEGALNKVYVSGIPDHTINNEAMVDHLVALVEKKAAEIEALEAARKAKEPATAAE

Sequences:

>Translated_384_residues
MSVRTYRDISRRVSRKIRVGSVFVGGDAPVSVQTMTNTLTTDVAATVAQIRRAQEAGADIVRVSCPDEESTAALKAIISQ
VEVPIVADIHFHYKRAIEAAEAGAACLRINPGNIGSAERVREVVRAAKDHGCSMRIGVNAGSLEKELLEKYGEPCPEAMV
ESALSHARILEDNDFTEFKISVKASDAFLAVAAYKALAEACDYPLHLGITEAGGLRGGTVKSAIGIGSMLWAGIGDTIRV
SLSAQPEEEVKVGFEILKSLNLRHRGVRVVSCPSCARQGFDVIKTVEVLESRLSHIQTPITLSIIGCVVNGPGEARETDI
GLTGGGNLAKEGALNKVYVSGIPDHTINNEAMVDHLVALVEKKAAEIEALEAARKAKEPATAAE
>Mature_383_residues
SVRTYRDISRRVSRKIRVGSVFVGGDAPVSVQTMTNTLTTDVAATVAQIRRAQEAGADIVRVSCPDEESTAALKAIISQV
EVPIVADIHFHYKRAIEAAEAGAACLRINPGNIGSAERVREVVRAAKDHGCSMRIGVNAGSLEKELLEKYGEPCPEAMVE
SALSHARILEDNDFTEFKISVKASDAFLAVAAYKALAEACDYPLHLGITEAGGLRGGTVKSAIGIGSMLWAGIGDTIRVS
LSAQPEEEVKVGFEILKSLNLRHRGVRVVSCPSCARQGFDVIKTVEVLESRLSHIQTPITLSIIGCVVNGPGEARETDIG
LTGGGNLAKEGALNKVYVSGIPDHTINNEAMVDHLVALVEKKAAEIEALEAARKAKEPATAAE

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=360, Percent_Identity=56.6666666666667, Blast_Score=407, Evalue=1e-115,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_RHORT (Q2RWE4)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_425838.1
- ProteinModelPortal:   Q2RWE4
- STRING:   Q2RWE4
- GeneID:   3834729
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A0747
- NMPDR:   fig|1085.1.peg.1598
- eggNOG:   COG0821
- HOGENOM:   HBG335271
- OMA:   APIVVQS
- PhylomeDB:   Q2RWE4
- ProtClustDB:   PRK00366
- BioCyc:   RRUB269796:RRU_A0747-MONOMER
- HAMAP:   MF_00159
- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF004640
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE; SSF51717 DHP_synth_like

EC number: =1.17.7.1

Molecular weight: Translated: 40786; Mature: 40655

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVRTYRDISRRVSRKIRVGSVFVGGDAPVSVQTMTNTLTTDVAATVAQIRRAQEAGADI
CCCCHHHHHHHHHHHHEEEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCE
VRVSCPDEESTAALKAIISQVEVPIVADIHFHYKRAIEAAEAGAACLRINPGNIGSAERV
EEEECCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHH
REVVRAAKDHGCSMRIGVNAGSLEKELLEKYGEPCPEAMVESALSHARILEDNDFTEFKI
HHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEE
SVKASDAFLAVAAYKALAEACDYPLHLGITEAGGLRGGTVKSAIGIGSMLWAGIGDTIRV
EEECCCHHHHHHHHHHHHHHCCCCEEECEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEE
SLSAQPEEEVKVGFEILKSLNLRHRGVRVVSCPSCARQGFDVIKTVEVLESRLSHIQTPI
EECCCCHHHHHHHHHHHHHCCCHHCCEEEEECCHHHHCCHHHHHHHHHHHHHHHHHCCCC
TLSIIGCVVNGPGEARETDIGLTGGGNLAKEGALNKVYVSGIPDHTINNEAMVDHLVALV
EEEEEEEEECCCCCCCCCCEEECCCCCCHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHH
EKKAAEIEALEAARKAKEPATAAE
HHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SVRTYRDISRRVSRKIRVGSVFVGGDAPVSVQTMTNTLTTDVAATVAQIRRAQEAGADI
CCCHHHHHHHHHHHHEEEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCE
VRVSCPDEESTAALKAIISQVEVPIVADIHFHYKRAIEAAEAGAACLRINPGNIGSAERV
EEEECCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHH
REVVRAAKDHGCSMRIGVNAGSLEKELLEKYGEPCPEAMVESALSHARILEDNDFTEFKI
HHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEE
SVKASDAFLAVAAYKALAEACDYPLHLGITEAGGLRGGTVKSAIGIGSMLWAGIGDTIRV
EEECCCHHHHHHHHHHHHHHCCCCEEECEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEE
SLSAQPEEEVKVGFEILKSLNLRHRGVRVVSCPSCARQGFDVIKTVEVLESRLSHIQTPI
EECCCCHHHHHHHHHHHHHCCCHHCCEEEEECCHHHHCCHHHHHHHHHHHHHHHHHCCCC
TLSIIGCVVNGPGEARETDIGLTGGGNLAKEGALNKVYVSGIPDHTINNEAMVDHLVALV
EEEEEEEEECCCCCCCCCCEEECCCCCCHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHH
EKKAAEIEALEAARKAKEPATAAE
HHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA