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The map label for this gene is nadE [H]

Identifier: 83592026

GI number: 83592026

Start: 811036

End: 812712

Strand: Direct

Name: nadE [H]

Synonym: Rru_A0687

Alternate gene names: 83592026

Gene position: 811036-812712 (Clockwise)

Preceding gene: 83592025

Following gene: 83592027

Centisome position: 18.63

GC content: 67.38

Gene sequence:

>1677_bases
ATGACCGGACCAGCCTCTTCCGAGCGCCTGACCCTGGCGCTCTGCCAGATCAATCCCTGGGTCGGTGACCTGTCGGGCAA
TCTGGAGCGCCTGCGCGCCGCCCGCGCCGAGGCCGCCCGTTTGGGGGCCGAGCTTGTTCTGGCCACCGAACTGGCCCTGA
CCGGCTATCCGCCCGAGGATCTGGTGCTGCGCGACAGCTTTATGGCCCAGGCGCGCGAGGTCGTCGCCACCCTGGCCGCC
GATACCGCCGATGGCGGGCCGGGCGTCATCGTCGGCGTGCCCTGGCGCAGCGAGGCGGGTGGGCGCCATAACGCGGCGCT
GCTGCTTGATAACGGGGCCATCGCCGGCATGGTCTTCAAGCATCATTTGCCCAATTACGGGGTATTCGACGAAAAGCGCG
TCTTCGATCCCGGCCCCAAGCCCGCGCCGCTGATCTTTCGCGGCTGGCGGCTGGGGGTGATGATCTGCGAGGACATGTGG
TTCCCCGATGTCGCCGCCGCGCTGGTCGCTGGCGGCGCCGAGATCTTGCTGGTGCCCAACGGCTCGCCCTTCGATCTGGA
AAAGACCGAGGCCCGCCTTGATCACGCCCGCGCCCGGGTCACCGAAAGCGGCCTGCCCCTGGTCTATGTCAATCAGGTGT
GCGGTCAGGACGAACTGGTCTTCGATGGCGCCTCGTTCGTCATGGGGGGCGATGGCGCCTTGCGGATGACGGTGGCCCCC
TGGCGCGAGGCGGTAAGCCCGGTGGTCTTTACGCGCAAGGATGGGCGGGTCGATACCCCGGCCCAACCGATCCCGCCGCC
GCCGCCGGTCGCCGCCGACATCTATCAGGCGATGGTGCTGGGCCTGCGCGATTATGTGACCAAGAACGGCTTTCCCGGGG
TGATCCTGGGTTTGTCGGGCGGCATCGACAGCGCGCTGTCGGCCGCCGTCGCCGTTGACGCCCTGGGGGCGGAGCGGGTG
TGGTGCGTGATGATGCCCAGCCCCTATACCAGCGCCGAAAGCCTGGAAGACGCCGCCGCCTGCGCCCGATTGCTTGGCAC
CCGGCTTGACACCATCGATATCGGCCCGGCGATGGCGGCCTTTGGCGCCATGTTGGAGCCCCATTTCGCCGGGCGCGCCG
CCGATATCACCGAAGAGAACCTGCAATCCCGGGCCCGGGGCCTGACCTTGATGGCGCTGTCGAATAAATTCGGCCCGATG
GTGTTGTCGACGGGCAATAAATCCGAGATGAGCGTTGGCTATGCTACGCTCTATGGCGATATGTGCGGCGGCTATTCGGT
GCTCAAGGACGTTTACAAAACCGCTGTCTTCGCCGTGTCGCGCTGGCGCAACGCCAACCATCCCGAGGGCGCCCTGGGGC
CCGAGGGTCCGGTGATGCCCGAGCGGGTGATCACCAAGGCGCCCAGCGCCGAGCTGCGCCCCGATCAGAAGGACGAGGAC
AGCCTGCCGCCCTATGTCGATCTTGATCGCGTGCTTCATCTGCTGATCGAGGAGCAGCGCGGCATCGCCGCCGCCACCGC
CGAAAGCGGTTTGCCGCGGCCTTTGGTCGAGCGGGTGTGGACGCTGCTGCAACGGGCGGAGTACAAACGCCGCCAAGCCC
CGCCGGGCGTCAAGATCAGCGCGCTTGCTTTCGGCCGCGACCGCCGCTATCCGATCACCCACGCCTACGGCGGTTAA

Upstream 100 bases:

>100_bases
CCGCCCTTCCCTTGACGCTGGGGGGATCGACCCTTAGCCTTTGAGCCTGCCGCTCCGGCCCGGGCCGGGGCATATCGTTC
CTTGTGCGGGACCCTCCCTG

Downstream 100 bases:

>100_bases
GCCGGGGCGTTCCCGGGCCATCAAGGAACCGTCATGTCGTCCGTTCTCGTGCGTTTCGCCCCCAGCCCCACCGGCCGGTT
GCACCTGGGCAATGCCCGCG

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 558; Mature: 557

Protein sequence:

>558_residues
MTGPASSERLTLALCQINPWVGDLSGNLERLRAARAEAARLGAELVLATELALTGYPPEDLVLRDSFMAQAREVVATLAA
DTADGGPGVIVGVPWRSEAGGRHNAALLLDNGAIAGMVFKHHLPNYGVFDEKRVFDPGPKPAPLIFRGWRLGVMICEDMW
FPDVAAALVAGGAEILLVPNGSPFDLEKTEARLDHARARVTESGLPLVYVNQVCGQDELVFDGASFVMGGDGALRMTVAP
WREAVSPVVFTRKDGRVDTPAQPIPPPPPVAADIYQAMVLGLRDYVTKNGFPGVILGLSGGIDSALSAAVAVDALGAERV
WCVMMPSPYTSAESLEDAAACARLLGTRLDTIDIGPAMAAFGAMLEPHFAGRAADITEENLQSRARGLTLMALSNKFGPM
VLSTGNKSEMSVGYATLYGDMCGGYSVLKDVYKTAVFAVSRWRNANHPEGALGPEGPVMPERVITKAPSAELRPDQKDED
SLPPYVDLDRVLHLLIEEQRGIAAATAESGLPRPLVERVWTLLQRAEYKRRQAPPGVKISALAFGRDRRYPITHAYGG

Sequences:

>Translated_558_residues
MTGPASSERLTLALCQINPWVGDLSGNLERLRAARAEAARLGAELVLATELALTGYPPEDLVLRDSFMAQAREVVATLAA
DTADGGPGVIVGVPWRSEAGGRHNAALLLDNGAIAGMVFKHHLPNYGVFDEKRVFDPGPKPAPLIFRGWRLGVMICEDMW
FPDVAAALVAGGAEILLVPNGSPFDLEKTEARLDHARARVTESGLPLVYVNQVCGQDELVFDGASFVMGGDGALRMTVAP
WREAVSPVVFTRKDGRVDTPAQPIPPPPPVAADIYQAMVLGLRDYVTKNGFPGVILGLSGGIDSALSAAVAVDALGAERV
WCVMMPSPYTSAESLEDAAACARLLGTRLDTIDIGPAMAAFGAMLEPHFAGRAADITEENLQSRARGLTLMALSNKFGPM
VLSTGNKSEMSVGYATLYGDMCGGYSVLKDVYKTAVFAVSRWRNANHPEGALGPEGPVMPERVITKAPSAELRPDQKDED
SLPPYVDLDRVLHLLIEEQRGIAAATAESGLPRPLVERVWTLLQRAEYKRRQAPPGVKISALAFGRDRRYPITHAYGG
>Mature_557_residues
TGPASSERLTLALCQINPWVGDLSGNLERLRAARAEAARLGAELVLATELALTGYPPEDLVLRDSFMAQAREVVATLAAD
TADGGPGVIVGVPWRSEAGGRHNAALLLDNGAIAGMVFKHHLPNYGVFDEKRVFDPGPKPAPLIFRGWRLGVMICEDMWF
PDVAAALVAGGAEILLVPNGSPFDLEKTEARLDHARARVTESGLPLVYVNQVCGQDELVFDGASFVMGGDGALRMTVAPW
REAVSPVVFTRKDGRVDTPAQPIPPPPPVAADIYQAMVLGLRDYVTKNGFPGVILGLSGGIDSALSAAVAVDALGAERVW
CVMMPSPYTSAESLEDAAACARLLGTRLDTIDIGPAMAAFGAMLEPHFAGRAADITEENLQSRARGLTLMALSNKFGPMV
LSTGNKSEMSVGYATLYGDMCGGYSVLKDVYKTAVFAVSRWRNANHPEGALGPEGPVMPERVITKAPSAELRPDQKDEDS
LPPYVDLDRVLHLLIEEQRGIAAATAESGLPRPLVERVWTLLQRAEYKRRQAPPGVKISALAFGRDRRYPITHAYGG

Specific function: It is not known if this NAD synthase uses NH(3) or glutamine as the N donor [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6321865, Length=373, Percent_Identity=26.5415549597855, Blast_Score=87, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 59667; Mature: 59536

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGPASSERLTLALCQINPWVGDLSGNLERLRAARAEAARLGAELVLATELALTGYPPED
CCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCHHEEEEEHHEECCCCCHH
LVLRDSFMAQAREVVATLAADTADGGPGVIVGVPWRSEAGGRHNAALLLDNGAIAGMVFK
HEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCEEEEEECCCEEEHHHH
HHLPNYGVFDEKRVFDPGPKPAPLIFRGWRLGVMICEDMWFPDVAAALVAGGAEILLVPN
HCCCCCCCCCCCCCCCCCCCCCCEEEECCEEEEEEECCCCCHHHHHHHHCCCCEEEEECC
GSPFDLEKTEARLDHARARVTESGLPLVYVNQVCGQDELVFDGASFVMGGDGALRMTVAP
CCCCCCHHHHHHHHHHHHHHHCCCCCEEEEHHHCCCCCEEECCCCEEECCCCEEEEEEHH
WREAVSPVVFTRKDGRVDTPAQPIPPPPPVAADIYQAMVLGLRDYVTKNGFPGVILGLSG
HHHCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECC
GIDSALSAAVAVDALGAERVWCVMMPSPYTSAESLEDAAACARLLGTRLDTIDIGPAMAA
CHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEECCHHHHH
FGAMLEPHFAGRAADITEENLQSRARGLTLMALSNKFGPMVLSTGNKSEMSVGYATLYGD
HHHHHCCCCCCCCCCCCHHHHHHHHCCEEEEEECCCCCCEEEECCCCCHHEECHHEEHHH
MCGGYSVLKDVYKTAVFAVSRWRNANHPEGALGPEGPVMPERVITKAPSAELRPDQKDED
HCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCC
SLPPYVDLDRVLHLLIEEQRGIAAATAESGLPRPLVERVWTLLQRAEYKRRQAPPGVKIS
CCCCCCCHHHHHHHHHHHCCCCEEEHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEE
ALAFGRDRRYPITHAYGG
EEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
TGPASSERLTLALCQINPWVGDLSGNLERLRAARAEAARLGAELVLATELALTGYPPED
CCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCHHEEEEEHHEECCCCCHH
LVLRDSFMAQAREVVATLAADTADGGPGVIVGVPWRSEAGGRHNAALLLDNGAIAGMVFK
HEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCEEEEEECCCEEEHHHH
HHLPNYGVFDEKRVFDPGPKPAPLIFRGWRLGVMICEDMWFPDVAAALVAGGAEILLVPN
HCCCCCCCCCCCCCCCCCCCCCCEEEECCEEEEEEECCCCCHHHHHHHHCCCCEEEEECC
GSPFDLEKTEARLDHARARVTESGLPLVYVNQVCGQDELVFDGASFVMGGDGALRMTVAP
CCCCCCHHHHHHHHHHHHHHHCCCCCEEEEHHHCCCCCEEECCCCEEECCCCEEEEEEHH
WREAVSPVVFTRKDGRVDTPAQPIPPPPPVAADIYQAMVLGLRDYVTKNGFPGVILGLSG
HHHCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECC
GIDSALSAAVAVDALGAERVWCVMMPSPYTSAESLEDAAACARLLGTRLDTIDIGPAMAA
CHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEECCHHHHH
FGAMLEPHFAGRAADITEENLQSRARGLTLMALSNKFGPMVLSTGNKSEMSVGYATLYGD
HHHHHCCCCCCCCCCCCHHHHHHHHCCEEEEEECCCCCCEEEECCCCCHHEECHHEEHHH
MCGGYSVLKDVYKTAVFAVSRWRNANHPEGALGPEGPVMPERVITKAPSAELRPDQKDED
HCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCC
SLPPYVDLDRVLHLLIEEQRGIAAATAESGLPRPLVERVWTLLQRAEYKRRQAPPGVKIS
CCCCCCCHHHHHHHHHHHCCCCEEEHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEE
ALAFGRDRRYPITHAYGG
EEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8431308; 8195100 [H]