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The map label for this gene is ysaA [C]

Identifier: 83591652

GI number: 83591652

Start: 380440

End: 380853

Strand: Direct

Name: ysaA [C]

Synonym: Rru_A0312

Alternate gene names: 83591652

Gene position: 380440-380853 (Clockwise)

Preceding gene: 83591651

Following gene: 83591653

Centisome position: 8.74

GC content: 66.18

Gene sequence:

>414_bases
ATGGCGCACGAGGAGGAATTCAATCCCTCGCGCTCCCGGGTCAGGGTGCTCGCCCGCACCGCCGGCGAAAAGGCCGTTCC
GGTCACCTGCCTGCAATGCGACGACGCCGGATGCATGGCCGTCTGCCCGGCCCATGCCATCACCGACGATGTCGTCACCG
GCGCCCGGCTGATCAACGAAACCCGCTGCATCAAATGCACGATGTGCATGCAGATCTGCGCCTTCGGGGCGACGTTCCAG
GATGAGCGCAGCCGCAAGATGCTGAAATGCGACCTCTGTGACGGCGATCCCCTCTGCGCCAAGGCCTGCCCGTCGGGCGC
CATCGTCAGCGGCGAGGCGGCGACCGCCAATGCGGCCAAGCGGCTGGTCGCCGCGCGCAAGATGAAAGTCTTTTCAAAAG
GAGGGCGCGGCTGA

Upstream 100 bases:

>100_bases
CGTTCGGCACCGATCCGACTTCAGGAAAAAAACGATGGCCGTCTTACTGATCACACCTGAGAAATGCACGAATTGCCGTG
CCTGCGAGGTCGCCTGTTCG

Downstream 100 bases:

>100_bases
TGTTTGGCTGGTGTGGACACCTGCTTCGCGTCGATCTGAGTACCGGCGCGATCCGCAAGGAGGCGCTCGAGGACGGCGCG
GCGAAAGCCCATCTGGGCGC

Product: 4Fe-4S ferredoxin, iron-sulfur binding

Products: CO2; NADH

Alternate protein names: Fe-S-Cluster-Containing Hydrogenase Subunit; Fe-S-Cluster; Fe-S Cluster-Containing Protein; 7Fe Ferredoxin; Oxidoreductase Iron-Sulfur Protein; Electron Transport Protein HydN; Formate Dehydrogenase Iron-Sulfur Subunit; Nitrate-Inducible Formate Dehydrogenase Beta Subunit; Carbon-Monoxide Dehydrogenase Fe-S Subunit; FAD/NADPH Dehydrogenase/Oxidoreductase Family Protein; 4Fe-4S Ferredoxin; Formate Dehydrogenase Subunit Beta; PhenylglyoxylateAcceptor Oxidoreductase; NADH Dependent Phenylglyoxylate; Formate Dehydrogenase Beta Subunit; Formate Hydrogenlyase Iron-Sulfur Subunit I

Number of amino acids: Translated: 137; Mature: 136

Protein sequence:

>137_residues
MAHEEEFNPSRSRVRVLARTAGEKAVPVTCLQCDDAGCMAVCPAHAITDDVVTGARLINETRCIKCTMCMQICAFGATFQ
DERSRKMLKCDLCDGDPLCAKACPSGAIVSGEAATANAAKRLVAARKMKVFSKGGRG

Sequences:

>Translated_137_residues
MAHEEEFNPSRSRVRVLARTAGEKAVPVTCLQCDDAGCMAVCPAHAITDDVVTGARLINETRCIKCTMCMQICAFGATFQ
DERSRKMLKCDLCDGDPLCAKACPSGAIVSGEAATANAAKRLVAARKMKVFSKGGRG
>Mature_136_residues
AHEEEFNPSRSRVRVLARTAGEKAVPVTCLQCDDAGCMAVCPAHAITDDVVTGARLINETRCIKCTMCMQICAFGATFQD
ERSRKMLKCDLCDGDPLCAKACPSGAIVSGEAATANAAKRLVAARKMKVFSKGGRG

Specific function: Unknown

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI2367245, Length=102, Percent_Identity=39.2156862745098, Blast_Score=64, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.2.1.2

Molecular weight: Translated: 14558; Mature: 14427

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

8.8 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
13.1 %Cys+Met (Translated Protein)
8.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
12.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHEEEFNPSRSRVRVLARTAGEKAVPVTCLQCDDAGCMAVCPAHAITDDVVTGARLINE
CCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCEEECCCHHHHHHHHHHHHHHCC
TRCIKCTMCMQICAFGATFQDERSRKMLKCDLCDGDPLCAKACPSGAIVSGEAATANAAK
CHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCHHHHHCCCCCEEECCCHHHHHHH
RLVAARKMKVFSKGGRG
HHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AHEEEFNPSRSRVRVLARTAGEKAVPVTCLQCDDAGCMAVCPAHAITDDVVTGARLINE
CCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCEEECCCHHHHHHHHHHHHHHCC
TRCIKCTMCMQICAFGATFQDERSRKMLKCDLCDGDPLCAKACPSGAIVSGEAATANAAK
CHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCHHHHHCCCCCEEECCCHHHHHHH
RLVAARKMKVFSKGGRG
HHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA