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The map label for this gene is fcl [C]

Identifier: 83591594

GI number: 83591594

Start: 315943

End: 316932

Strand: Direct

Name: fcl [C]

Synonym: Rru_A0254

Alternate gene names: 83591594

Gene position: 315943-316932 (Clockwise)

Preceding gene: 83591593

Following gene: 83591595

Centisome position: 7.26

GC content: 65.56

Gene sequence:

>990_bases
ATGACTGAGCCCCGCCCCGCGCCCACTTCACGCCCCCTCGGCCAGCCCTTCGATTTGACGGGCAAGCGCGTTTGGGTCGC
CGGTCATCGGGGACTGGTGGGCGGCGCGGTCGTGCGCCGTTTGGCGCGCGAGGACTGCGCGGTGCTCTGCGTCGGTCGCG
AGGACCTCGATCTAACCCGCCAACAGGCGGTCGAGGCGTGGATGGAGGCCAATCGGCCCGATGCGGTGGTGATGGCCGCC
GCCCTGGTTGGCGGCATCAAGGCCAATGATCGGCGCTCGGCCGAATTCATCCACCAGAATCTGGCGGTTCAGACCAATAT
CATCCATGCCGCTTGGCAAGCCGGCGTGGGCAAGGTTCTGTTCCTGGGATCGTCGTGCATCTATCCGCGCGATGTCGCCC
AGCCGATGCGCGAGGACGCCCTGCTCAGCGGGCCGCTCGAACCGACCAACCAGTGGTACGCCATCGCCAAGATCGCCGGC
ATCCGCATGGCCCAGGCCTATCGCCGCCAATATGGCTGCGATTACATCTCGGCGATGCCGACCAATCTTTATGGTCCGGG
CGACAACTTCGATCTTGATGGCGGGCATGTGCTGCCCGCGCTGCTGCGCAAGATCCACGAAGCCAAGGTCGAGGGCCGGG
GCGAGGTGGTGCTGTGGGGCAGCGGCGCCCCCTTGCGCGAATTCCTCTATGTCGATGATCTGGCCGATGCCCTGGTCTTC
CTGCTCAAGGCGTATTCGGCCGATGATCACATCAACGTCGGCAGCGGCGAGGAAATCACCATCAAGGCCCTGGCCGAGAC
CATCGCTGGGGTGGTGGGCTATGAGGGGCGGTTCGTTTTCGATACCACCATGCCCGATGGCACGCCGCGTAAGTTGATGG
ACAGCGGCCGCCTTGCCGCCTTGGGCTGGCGACCGGCGACCGACCTGCGCAGCGGCATCGCCGCCACCTATCGCTGGTTC
CTCGACAACGCCGAGCGGCTGCGTCAATAA

Upstream 100 bases:

>100_bases
CTGGGCTGGGTCGCCGAAACCCCGCTTGCCGAGTTGGTCGCTGAAATGGTCGCCGCCGATCTCGCCGTGCTCAAACGCAC
CCAGGAGAGTCTGATCACCA

Downstream 100 bases:

>100_bases
GGGTTCCCAGAGCGGGATGGCGAAAAGTGTGCGCGGTTTTCGCCTAAAATCCCGCTCTAACATTTAGATTTAGATCAGGA
TTCAGATTTCAGGTCGATCA

Product: NAD-dependent epimerase/dehydratase

Products: GDP-4-dehydro-6-deoxy-D-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MTEPRPAPTSRPLGQPFDLTGKRVWVAGHRGLVGGAVVRRLAREDCAVLCVGREDLDLTRQQAVEAWMEANRPDAVVMAA
ALVGGIKANDRRSAEFIHQNLAVQTNIIHAAWQAGVGKVLFLGSSCIYPRDVAQPMREDALLSGPLEPTNQWYAIAKIAG
IRMAQAYRRQYGCDYISAMPTNLYGPGDNFDLDGGHVLPALLRKIHEAKVEGRGEVVLWGSGAPLREFLYVDDLADALVF
LLKAYSADDHINVGSGEEITIKALAETIAGVVGYEGRFVFDTTMPDGTPRKLMDSGRLAALGWRPATDLRSGIAATYRWF
LDNAERLRQ

Sequences:

>Translated_329_residues
MTEPRPAPTSRPLGQPFDLTGKRVWVAGHRGLVGGAVVRRLAREDCAVLCVGREDLDLTRQQAVEAWMEANRPDAVVMAA
ALVGGIKANDRRSAEFIHQNLAVQTNIIHAAWQAGVGKVLFLGSSCIYPRDVAQPMREDALLSGPLEPTNQWYAIAKIAG
IRMAQAYRRQYGCDYISAMPTNLYGPGDNFDLDGGHVLPALLRKIHEAKVEGRGEVVLWGSGAPLREFLYVDDLADALVF
LLKAYSADDHINVGSGEEITIKALAETIAGVVGYEGRFVFDTTMPDGTPRKLMDSGRLAALGWRPATDLRSGIAATYRWF
LDNAERLRQ
>Mature_328_residues
TEPRPAPTSRPLGQPFDLTGKRVWVAGHRGLVGGAVVRRLAREDCAVLCVGREDLDLTRQQAVEAWMEANRPDAVVMAAA
LVGGIKANDRRSAEFIHQNLAVQTNIIHAAWQAGVGKVLFLGSSCIYPRDVAQPMREDALLSGPLEPTNQWYAIAKIAGI
RMAQAYRRQYGCDYISAMPTNLYGPGDNFDLDGGHVLPALLRKIHEAKVEGRGEVVLWGSGAPLREFLYVDDLADALVFL
LKAYSADDHINVGSGEEITIKALAETIAGVVGYEGRFVFDTTMPDGTPRKLMDSGRLAALGWRPATDLRSGIAATYRWFL
DNAERLRQ

Specific function: Putative nucleotide sugar epimerase/dehydrogenase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=316, Percent_Identity=34.4936708860759, Blast_Score=196, Evalue=2e-50,
Organism=Homo sapiens, GI7657641, Length=348, Percent_Identity=23.2758620689655, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI42516563, Length=320, Percent_Identity=25.625, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1788365, Length=320, Percent_Identity=52.5, Blast_Score=343, Evalue=1e-95,
Organism=Escherichia coli, GI1788353, Length=352, Percent_Identity=23.5795454545455, Blast_Score=68, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17554428, Length=315, Percent_Identity=29.8412698412698, Blast_Score=164, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI115532424, Length=269, Percent_Identity=25.6505576208178, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI19922778, Length=320, Percent_Identity=32.1875, Blast_Score=187, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 1.1.1.271

Molecular weight: Translated: 35999; Mature: 35867

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPRPAPTSRPLGQPFDLTGKRVWVAGHRGLVGGAVVRRLAREDCAVLCVGREDLDLTR
CCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHH
QQAVEAWMEANRPDAVVMAAALVGGIKANDRRSAEFIHQNLAVQTNIIHAAWQAGVGKVL
HHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
FLGSSCIYPRDVAQPMREDALLSGPLEPTNQWYAIAKIAGIRMAQAYRRQYGCDYISAMP
EECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCHHHHCC
TNLYGPGDNFDLDGGHVLPALLRKIHEAKVEGRGEVVLWGSGAPLREFLYVDDLADALVF
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHH
LLKAYSADDHINVGSGEEITIKALAETIAGVVGYEGRFVFDTTMPDGTPRKLMDSGRLAA
HHHHHCCCCCEECCCCCEEHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHCCCCEEE
LGWRPATDLRSGIAATYRWFLDNAERLRQ
ECCCCHHHHHHHHHHHHHHHHCCHHHHCC
>Mature Secondary Structure 
TEPRPAPTSRPLGQPFDLTGKRVWVAGHRGLVGGAVVRRLAREDCAVLCVGREDLDLTR
CCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHH
QQAVEAWMEANRPDAVVMAAALVGGIKANDRRSAEFIHQNLAVQTNIIHAAWQAGVGKVL
HHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
FLGSSCIYPRDVAQPMREDALLSGPLEPTNQWYAIAKIAGIRMAQAYRRQYGCDYISAMP
EECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCHHHHCC
TNLYGPGDNFDLDGGHVLPALLRKIHEAKVEGRGEVVLWGSGAPLREFLYVDDLADALVF
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHH
LLKAYSADDHINVGSGEEITIKALAETIAGVVGYEGRFVFDTTMPDGTPRKLMDSGRLAA
HHHHHCCCCCEECCCCCEEHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHCCCCEEE
LGWRPATDLRSGIAATYRWFLDNAERLRQ
ECCCCHHHHHHHHHHHHHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDP-L-fucose; NADP+

Specific reaction: GDP-L-fucose + NADP+ = GDP-4-dehydro-6-deoxy-D-mannose + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]