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The map label for this gene is noeL [H]

Identifier: 83591593

GI number: 83591593

Start: 314880

End: 315950

Strand: Direct

Name: noeL [H]

Synonym: Rru_A0253

Alternate gene names: 83591593

Gene position: 314880-315950 (Clockwise)

Preceding gene: 83591580

Following gene: 83591594

Centisome position: 7.23

GC content: 61.34

Gene sequence:

>1071_bases
ATGGCGGCGAAGGTTGCGCTTATTACCGGGGTGACGGGGCAGGACGGCGCCTATCTGGCGGAATTCCTGCTGGGCAAGGG
CTATGAGGTCCACGGCATCAAGCGGCGGTCGTCGTCGCTCAACACCGGGCGGATCGATCCGCTTTATCAAGATCCCCACG
ATCCCGACCGCACGTTCTTCCTGCATTACGGCGACATGACCGACAGCGCCAATCTGATCGGGCTGGTTCAGGCCATTCAG
CCCGACGAGATCTATAATCTCGCCGCGCAAAGCCATGTCCAGGTCAGTTTCGAGATCCCCGAATACACCGCCAATGCCGA
TGCGGTCGGCACCTTGCGCCTGCTCGAGGCGATCCGTTTGCTTGGGCTGACCGACAAGACGCGGTTCTATCAGGCCTCGA
CTTCCGAGCTTTACGGCAAGGTGCGCGAGACGCCGCAAAGTGAAACCACGCCGTTTTATCCGCGCAGCCCCTATGCGGCG
GCCAAGCTTTACGCCTATTGGATCACGGTGAACTACCGCGAGGCCTATGGCATTCACGCCTCGAACGGCATTCTTTTCAA
TCATGAAAGCCCGCTGCGCGGCGAAACCTTCGTGACCCGCAAGATCACCCGGGCGGTGGCGGCCATTCATCTTGGACTGC
AAGACGTGCTTTATCTGGGCAATCTCGACAGCTGGCGTGACTGGGGCCATGCGCGCGACTATGTGAAAGGCATGTGGGCG
ATGCTTCAACAACCCCGCCCCGATGACTATGTCTTGGCGACGGGGGTGGTCCATTCGGTTCGCGCCTTCGTGGAAATGGC
TTTTTCCAAGCTGGGGCGCCGCGTGACATGGCAGGGCGAGGGGGTCGACGAGATCGGGATCGACAGCGCCAGCGGCGCCA
AACTGGTGGCCATCGATCCCCGTTATTTCCGGCCGACCGAGGTCGAGTTCCTGCAAGGCGATCCGACCAAGGCCCGCGAG
CGTCTGGGCTGGGTCGCCGAAACCCCGCTTGCCGAGTTGGTCGCTGAAATGGTCGCCGCCGATCTCGCCGTGCTCAAACG
CACCCAGGAGAGTCTGATCACCAATGACTGA

Upstream 100 bases:

>100_bases
CGGGGAGGGAAAACGCGCCGTTTCCCAAAGGCCGGCTTGCTCGGCTAGGATCGTTCGCGGCACGTGGTGTGCGCAGGCCA
GAACGGCAAAGGAGCGGACG

Downstream 100 bases:

>100_bases
GCCCCGCCCCGCGCCCACTTCACGCCCCCTCGGCCAGCCCTTCGATTTGACGGGCAAGCGCGTTTGGGTCGCCGGTCATC
GGGGACTGGTGGGCGGCGCG

Product: GDP-mannose 4,6-dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MAAKVALITGVTGQDGAYLAEFLLGKGYEVHGIKRRSSSLNTGRIDPLYQDPHDPDRTFFLHYGDMTDSANLIGLVQAIQ
PDEIYNLAAQSHVQVSFEIPEYTANADAVGTLRLLEAIRLLGLTDKTRFYQASTSELYGKVRETPQSETTPFYPRSPYAA
AKLYAYWITVNYREAYGIHASNGILFNHESPLRGETFVTRKITRAVAAIHLGLQDVLYLGNLDSWRDWGHARDYVKGMWA
MLQQPRPDDYVLATGVVHSVRAFVEMAFSKLGRRVTWQGEGVDEIGIDSASGAKLVAIDPRYFRPTEVEFLQGDPTKARE
RLGWVAETPLAELVAEMVAADLAVLKRTQESLITND

Sequences:

>Translated_356_residues
MAAKVALITGVTGQDGAYLAEFLLGKGYEVHGIKRRSSSLNTGRIDPLYQDPHDPDRTFFLHYGDMTDSANLIGLVQAIQ
PDEIYNLAAQSHVQVSFEIPEYTANADAVGTLRLLEAIRLLGLTDKTRFYQASTSELYGKVRETPQSETTPFYPRSPYAA
AKLYAYWITVNYREAYGIHASNGILFNHESPLRGETFVTRKITRAVAAIHLGLQDVLYLGNLDSWRDWGHARDYVKGMWA
MLQQPRPDDYVLATGVVHSVRAFVEMAFSKLGRRVTWQGEGVDEIGIDSASGAKLVAIDPRYFRPTEVEFLQGDPTKARE
RLGWVAETPLAELVAEMVAADLAVLKRTQESLITND
>Mature_355_residues
AAKVALITGVTGQDGAYLAEFLLGKGYEVHGIKRRSSSLNTGRIDPLYQDPHDPDRTFFLHYGDMTDSANLIGLVQAIQP
DEIYNLAAQSHVQVSFEIPEYTANADAVGTLRLLEAIRLLGLTDKTRFYQASTSELYGKVRETPQSETTPFYPRSPYAAA
KLYAYWITVNYREAYGIHASNGILFNHESPLRGETFVTRKITRAVAAIHLGLQDVLYLGNLDSWRDWGHARDYVKGMWAM
LQQPRPDDYVLATGVVHSVRAFVEMAFSKLGRRVTWQGEGVDEIGIDSASGAKLVAIDPRYFRPTEVEFLQGDPTKARER
LGWVAETPLAELVAEMVAADLAVLKRTQESLITND

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=343, Percent_Identity=60.932944606414, Blast_Score=452, Evalue=1e-127,
Organism=Escherichia coli, GI1788366, Length=355, Percent_Identity=67.887323943662, Blast_Score=501, Evalue=1e-143,
Organism=Escherichia coli, GI48994969, Length=245, Percent_Identity=28.5714285714286, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1788353, Length=213, Percent_Identity=28.169014084507, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17539424, Length=347, Percent_Identity=63.6887608069164, Blast_Score=467, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI133901790, Length=347, Percent_Identity=63.6887608069164, Blast_Score=467, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI17539422, Length=347, Percent_Identity=63.6887608069164, Blast_Score=467, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI133901788, Length=347, Percent_Identity=63.6887608069164, Blast_Score=467, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI17507723, Length=345, Percent_Identity=64.3478260869565, Blast_Score=466, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI133901786, Length=347, Percent_Identity=63.6887608069164, Blast_Score=466, Evalue=1e-132,
Organism=Drosophila melanogaster, GI24158427, Length=347, Percent_Identity=61.9596541786743, Blast_Score=465, Evalue=1e-131,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 39704; Mature: 39573

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKVALITGVTGQDGAYLAEFLLGKGYEVHGIKRRSSSLNTGRIDPLYQDPHDPDRTFF
CCCEEEEEECCCCCCHHHHHHHHHCCCCEEEEEHHCCCCCCCCCCCCCCCCCCCCCCEEE
LHYGDMTDSANLIGLVQAIQPDEIYNLAAQSHVQVSFEIPEYTANADAVGTLRLLEAIRL
EEECCCCCCCHHHHHHHHCCCHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHHHHHH
LGLTDKTRFYQASTSELYGKVRETPQSETTPFYPRSPYAAAKLYAYWITVNYREAYGIHA
HCCCCCHHEEECCHHHHHHHHHCCCCCCCCCCCCCCCCHHEEEEEEEEEEEEHHCCCEEC
SNGILFNHESPLRGETFVTRKITRAVAAIHLGLQDVLYLGNLDSWRDWGHARDYVKGMWA
CCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
MLQQPRPDDYVLATGVVHSVRAFVEMAFSKLGRRVTWQGEGVDEIGIDSASGAKLVAIDP
HHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCEEEEECC
RYFRPTEVEFLQGDPTKARERLGWVAETPLAELVAEMVAADLAVLKRTQESLITND
CCCCCCCEEEECCCCHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AAKVALITGVTGQDGAYLAEFLLGKGYEVHGIKRRSSSLNTGRIDPLYQDPHDPDRTFF
CCEEEEEECCCCCCHHHHHHHHHCCCCEEEEEHHCCCCCCCCCCCCCCCCCCCCCCEEE
LHYGDMTDSANLIGLVQAIQPDEIYNLAAQSHVQVSFEIPEYTANADAVGTLRLLEAIRL
EEECCCCCCCHHHHHHHHCCCHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHHHHHH
LGLTDKTRFYQASTSELYGKVRETPQSETTPFYPRSPYAAAKLYAYWITVNYREAYGIHA
HCCCCCHHEEECCHHHHHHHHHCCCCCCCCCCCCCCCCHHEEEEEEEEEEEEHHCCCEEC
SNGILFNHESPLRGETFVTRKITRAVAAIHLGLQDVLYLGNLDSWRDWGHARDYVKGMWA
CCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
MLQQPRPDDYVLATGVVHSVRAFVEMAFSKLGRRVTWQGEGVDEIGIDSASGAKLVAIDP
HHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCEEEEECC
RYFRPTEVEFLQGDPTKARERLGWVAETPLAELVAEMVAADLAVLKRTQESLITND
CCCCCCCEEEECCCCHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10065558 [H]