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The map label for this gene is emrB [H]

Identifier: 83591497

GI number: 83591497

Start: 181624

End: 183198

Strand: Direct

Name: emrB [H]

Synonym: Rru_A0157

Alternate gene names: 83591497

Gene position: 181624-183198 (Clockwise)

Preceding gene: 83591496

Following gene: 83591501

Centisome position: 4.17

GC content: 64.25

Gene sequence:

>1575_bases
ATGGCCGCCGCCCCCGCTTCCGCCGCCGTCTCCGGCGGTGCCTTGCCCTTGCGCGATCGCCTTGGCTTCTTCGCCCTGGT
GGTCGGCATGTTCATGGCCATCCTCGATATCCAGATCGTCGCCAGTTCCCTGGCCGAGATCCAGGCCGGCGTCAGTGCCG
GTCCCGAAGAGATCAGCTGGGTACAGACCGGCTATCTAATCGCCGAAGTGGTGATGATTCCGCTGTCGGGTTGGCTGTCG
CGGTTGATGTCGACGCGCTGGCTGTTCACCATGGCCAGCGGCGGCTTCACCATCGCCAGCGGGCTGTGCGCCATCGCCTG
GAATCTCGAAAGCCTGGTTGCCTTCCGCGTCGTTCAGGGATTCCTGGGCGGGGCGATGATCCCCACCGTCTTCGCCGCCG
CCTTCGCCCTGTTCCCGGGCAAGAAGCAGGCCGGGGTGTCGGTGGTCATCGGCTTGGTGGCGACCTGCGCCCCGGCCCTG
GGGCCGACCATCGGCGGCCATCTGACCGAGGCCCTGTCCTGGCACTGGCTATTTTTGCTCAACCTGCCGGTCGGCGCCGC
GGTGACGGTCGCCGCGGCCACCCTGGTTCATTTCGACGAGGCCGATCCCAGCTTGCTGCGCCGTATCGACCTGATCGGCA
TCGGTCTGGTCGCGGTCTGCCTGGGAAGCTTGCAGTATATTCTGGAGGAAGGACCGCGCGACGACTGGTTCGACTCGGCG
CCCATCGTCTTTTTCACCGCCTGCGCGGCGATCGGCGGCGTTTTGCTGGTTTGGCGCGAACTGACGGCCAAGGACCCGGT
GGTCGATCTGCGCGCCTTCCTTGATCGCAATTTCGTTCTTGGCTGTCTTTACAGCTTCATCACCGGCGTCGGGCTTTATG
GGGCGGTCTATCTGATGCCGCTGTTCCTCGCCCGCGTCCGCGATCTCAATCCGGCGCAGATCGGCGAGGTGGTGATGATC
ACCGGCCTGTTCCAGTTTCTGTCGGCGCCGCTGGCCGGAGCGCTGTCGCGCAAGCTTGATCTGCGGGTGATGCTGTCGTT
CGGGTTGTTGATGTTCGCCCTTGGCCTCTGGCTGAACACCCATCTGACCCATGACTGGGACTATTGGGAATTCTTCCTGC
CCCAGGCGGTGCGCGGGGTGTCGTTGATGTTCATCTTCATCCCGATCAATCGCCTGAGCCTGGGCACCCTGCCCAAGGAG
AAGCTGGGCAACGCCTCGGGCCTTTATAATCTGATGCGCAATCTGGGCGGGGCGATCGGGCTGGCGGCGATCACCACCAT
GCTGACCGATCAGACCAAGATCCATTATTCCTATCTGGCCGAAAACGTCACCACCGCCCGGCTGGCCGCCGAGGCGACCC
TGTCGGGCATGGAGCGCCATTTCGATCCGGTTCTTGGCAGTTCCTCGGCCCAGGCGGCGCTGGGACAGGTTCGTCAGATC
GTGATGCGCGAGGCGACGGTGATGGCCTTCGCCGATGTCTTCACCGCCGTCGCCGTGGTCTTCGCCTTCGGCCTGCTGCT
GATGCCTTTCGTCCACAAGGTTGGCGGCAATGGTCCCGATGGCGGCGGCCATTGA

Upstream 100 bases:

>100_bases
CCGTGGCGGGCGCGATGTCGCGCTGGGTCTGGTCGGCACCGCCCTGGCCGGTTCGGCCGGGTCTTCCCCCACCCACCGCT
AAGTCGGGGCGCCCCGTTCC

Downstream 100 bases:

>100_bases
AGGATCCCAAGGGATCTGAACCAGATCGCGACGACGATTTGCTTCGACATCAAAGGGGTGGAGCGGCGGTAAAGGGGGGC
GCCCTTGCCGCCGCTTGATC

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 524; Mature: 523

Protein sequence:

>524_residues
MAAAPASAAVSGGALPLRDRLGFFALVVGMFMAILDIQIVASSLAEIQAGVSAGPEEISWVQTGYLIAEVVMIPLSGWLS
RLMSTRWLFTMASGGFTIASGLCAIAWNLESLVAFRVVQGFLGGAMIPTVFAAAFALFPGKKQAGVSVVIGLVATCAPAL
GPTIGGHLTEALSWHWLFLLNLPVGAAVTVAAATLVHFDEADPSLLRRIDLIGIGLVAVCLGSLQYILEEGPRDDWFDSA
PIVFFTACAAIGGVLLVWRELTAKDPVVDLRAFLDRNFVLGCLYSFITGVGLYGAVYLMPLFLARVRDLNPAQIGEVVMI
TGLFQFLSAPLAGALSRKLDLRVMLSFGLLMFALGLWLNTHLTHDWDYWEFFLPQAVRGVSLMFIFIPINRLSLGTLPKE
KLGNASGLYNLMRNLGGAIGLAAITTMLTDQTKIHYSYLAENVTTARLAAEATLSGMERHFDPVLGSSSAQAALGQVRQI
VMREATVMAFADVFTAVAVVFAFGLLLMPFVHKVGGNGPDGGGH

Sequences:

>Translated_524_residues
MAAAPASAAVSGGALPLRDRLGFFALVVGMFMAILDIQIVASSLAEIQAGVSAGPEEISWVQTGYLIAEVVMIPLSGWLS
RLMSTRWLFTMASGGFTIASGLCAIAWNLESLVAFRVVQGFLGGAMIPTVFAAAFALFPGKKQAGVSVVIGLVATCAPAL
GPTIGGHLTEALSWHWLFLLNLPVGAAVTVAAATLVHFDEADPSLLRRIDLIGIGLVAVCLGSLQYILEEGPRDDWFDSA
PIVFFTACAAIGGVLLVWRELTAKDPVVDLRAFLDRNFVLGCLYSFITGVGLYGAVYLMPLFLARVRDLNPAQIGEVVMI
TGLFQFLSAPLAGALSRKLDLRVMLSFGLLMFALGLWLNTHLTHDWDYWEFFLPQAVRGVSLMFIFIPINRLSLGTLPKE
KLGNASGLYNLMRNLGGAIGLAAITTMLTDQTKIHYSYLAENVTTARLAAEATLSGMERHFDPVLGSSSAQAALGQVRQI
VMREATVMAFADVFTAVAVVFAFGLLLMPFVHKVGGNGPDGGGH
>Mature_523_residues
AAAPASAAVSGGALPLRDRLGFFALVVGMFMAILDIQIVASSLAEIQAGVSAGPEEISWVQTGYLIAEVVMIPLSGWLSR
LMSTRWLFTMASGGFTIASGLCAIAWNLESLVAFRVVQGFLGGAMIPTVFAAAFALFPGKKQAGVSVVIGLVATCAPALG
PTIGGHLTEALSWHWLFLLNLPVGAAVTVAAATLVHFDEADPSLLRRIDLIGIGLVAVCLGSLQYILEEGPRDDWFDSAP
IVFFTACAAIGGVLLVWRELTAKDPVVDLRAFLDRNFVLGCLYSFITGVGLYGAVYLMPLFLARVRDLNPAQIGEVVMIT
GLFQFLSAPLAGALSRKLDLRVMLSFGLLMFALGLWLNTHLTHDWDYWEFFLPQAVRGVSLMFIFIPINRLSLGTLPKEK
LGNASGLYNLMRNLGGAIGLAAITTMLTDQTKIHYSYLAENVTTARLAAEATLSGMERHFDPVLGSSSAQAALGQVRQIV
MREATVMAFADVFTAVAVVFAFGLLLMPFVHKVGGNGPDGGGH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=478, Percent_Identity=30.3347280334728, Blast_Score=237, Evalue=1e-63,
Organism=Escherichia coli, GI1788710, Length=483, Percent_Identity=28.1573498964803, Blast_Score=217, Evalue=2e-57,
Organism=Escherichia coli, GI1790195, Length=434, Percent_Identity=26.036866359447, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI87081983, Length=428, Percent_Identity=25.7009345794392, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788392, Length=447, Percent_Identity=27.2930648769575, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6325455, Length=130, Percent_Identity=30, Blast_Score=67, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6319770, Length=390, Percent_Identity=22.5641025641026, Blast_Score=63, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55843; Mature: 55712

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
AAAPASAAVSGGALPLRDRLGFFALVVGMFMAILDIQIVASSLAEIQAGVSAGPEEISW
CCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]