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The map label for this gene is dnaN [H]

Identifier: 83591342

GI number: 83591342

Start: 1992

End: 3110

Strand: Direct

Name: dnaN [H]

Synonym: Rru_A0002

Alternate gene names: 83591342

Gene position: 1992-3110 (Clockwise)

Preceding gene: 83591341

Following gene: 83591343

Centisome position: 0.05

GC content: 64.61

Gene sequence:

>1119_bases
ATGAAGCTGACGATCGAGCGCACCGCCCTTCTCAAGTCCTTGGGACACGTCCAGAGCGTCGTCGAGCGGCGCAATACGAT
TCCCATCTTGTCGAACGTGCGGATGGAAACCGGCGAGAGCGGCTTCAGCCTGAACGCCACCGACATGGACCTGGAGATCG
TCGAGACGGTGACGGCGACCACCCTGGCCCCGGGAGCGACCACGGCGCCGGCCCATACCCTGTATGACATCGTCCGCAAG
CTGCCCGACGGCAGCCAGGTGGAATTGAGCTATGACGGCAACGACGGCCAGCTGGTGCTCAAGGCCGGGCGCTCGCGGTT
TTCGCTGTCGTGCCTGCCGGTCGAGGATTTTCCCGCGCTCAGCGCCGGCGAGTTCAGTCACAGCTTCTCGCTGCCGGCGA
CCGATCTGCGCGGTCTGATCGATCGCACGCGCTTTGCCATCTCCACCGAGGAAACCCGCTACTACCTCAACGGCATCTAT
CTGCACGCCACGGAAAGCGCCGGGGTGCCGGTGTTGCGCGCCGTGGCCACCGATGGCCACCGTTTGGCCCGCGCCGAGAT
TCCCTTGCCCGCCGGCGCCGCCGGCATGCCCGGGGTGATCGTGCCGCGCAAGACCGTGGGCGAATTGCGCAAGCTGATCG
AAGAGACCGGCATGGATATCACCGTCGGGCTGTCCGAGCAGAAGATCCGCTTCGATTTCGACGAAACCCGCCTGACCTCC
AAACTGATCGACGGCACTTTCCCCGATTACGAGCGCGTGATCCCGACCGAAAACGACAAGGTTCTGGAAGTCGATTGCAA
GCTTTTCGCCCAGGCCGCCGACCGGGTGTCGGCGATCTCGACGGAAAAGTCCCGGGCCATCAAGGTTGCCGTCGCCAAGG
GTACGATGACGCTTTCGGCTTCGAGTCCCGATGCCGGCAGCGCCGTCGAGGAGATCGAGGCGGCCTATCAGTCGACCTCG
CTCGAGATCGGCTTCAATTCCCGCTACCTGCTCGACATTCTCGGTCAGGTCGAGGGGGGATCGGTGCGCCTGACCTTTGC
CGACGCCGCCTCGCCGACGGTCATTCGCGACGTCGCCGACGCCTCGGCCGTCTATGTGCTGATGCCGATGCGGGTGTGA

Upstream 100 bases:

>100_bases
CTTCCCGCCCTTTCGGCGCTGTCTCCCGTCGCCGATCTCCCCTTCCCGTGCGGTCGGTCCCCCGGGGGCCAACCGTCCCG
ACACCGGTTCAGGCCCGCCC

Downstream 100 bases:

>100_bases
CGACGCCGCCCCTTTCCGCCGCGTCCGACCGGCCGCCCCCTGGAGGGCCAAGGGGCGTCGTGGTCGAGCGGCTGACCCTG
ACCGCCTTTCGCTGCTACGA

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MKLTIERTALLKSLGHVQSVVERRNTIPILSNVRMETGESGFSLNATDMDLEIVETVTATTLAPGATTAPAHTLYDIVRK
LPDGSQVELSYDGNDGQLVLKAGRSRFSLSCLPVEDFPALSAGEFSHSFSLPATDLRGLIDRTRFAISTEETRYYLNGIY
LHATESAGVPVLRAVATDGHRLARAEIPLPAGAAGMPGVIVPRKTVGELRKLIEETGMDITVGLSEQKIRFDFDETRLTS
KLIDGTFPDYERVIPTENDKVLEVDCKLFAQAADRVSAISTEKSRAIKVAVAKGTMTLSASSPDAGSAVEEIEAAYQSTS
LEIGFNSRYLLDILGQVEGGSVRLTFADAASPTVIRDVADASAVYVLMPMRV

Sequences:

>Translated_372_residues
MKLTIERTALLKSLGHVQSVVERRNTIPILSNVRMETGESGFSLNATDMDLEIVETVTATTLAPGATTAPAHTLYDIVRK
LPDGSQVELSYDGNDGQLVLKAGRSRFSLSCLPVEDFPALSAGEFSHSFSLPATDLRGLIDRTRFAISTEETRYYLNGIY
LHATESAGVPVLRAVATDGHRLARAEIPLPAGAAGMPGVIVPRKTVGELRKLIEETGMDITVGLSEQKIRFDFDETRLTS
KLIDGTFPDYERVIPTENDKVLEVDCKLFAQAADRVSAISTEKSRAIKVAVAKGTMTLSASSPDAGSAVEEIEAAYQSTS
LEIGFNSRYLLDILGQVEGGSVRLTFADAASPTVIRDVADASAVYVLMPMRV
>Mature_372_residues
MKLTIERTALLKSLGHVQSVVERRNTIPILSNVRMETGESGFSLNATDMDLEIVETVTATTLAPGATTAPAHTLYDIVRK
LPDGSQVELSYDGNDGQLVLKAGRSRFSLSCLPVEDFPALSAGEFSHSFSLPATDLRGLIDRTRFAISTEETRYYLNGIY
LHATESAGVPVLRAVATDGHRLARAEIPLPAGAAGMPGVIVPRKTVGELRKLIEETGMDITVGLSEQKIRFDFDETRLTS
KLIDGTFPDYERVIPTENDKVLEVDCKLFAQAADRVSAISTEKSRAIKVAVAKGTMTLSASSPDAGSAVEEIEAAYQSTS
LEIGFNSRYLLDILGQVEGGSVRLTFADAASPTVIRDVADASAVYVLMPMRV

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=375, Percent_Identity=39.4666666666667, Blast_Score=271, Evalue=4e-74,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40084; Mature: 40084

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTIERTALLKSLGHVQSVVERRNTIPILSNVRMETGESGFSLNATDMDLEIVETVTAT
CEEEEHHHHHHHHHHHHHHHHHHCCCCCEEECCEEECCCCCCEECCCCCCHHHHHHHHHH
TLAPGATTAPAHTLYDIVRKLPDGSQVELSYDGNDGQLVLKAGRSRFSLSCLPVEDFPAL
EECCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEECCCEEEEEEECCCCCCCC
SAGEFSHSFSLPATDLRGLIDRTRFAISTEETRYYLNGIYLHATESAGVPVLRAVATDGH
CCCCCCCCCCCCHHHHHHHHHHHHEEEECCCCEEEEEEEEEEEECCCCCCEEEEECCCCC
RLARAEIPLPAGAAGMPGVIVPRKTVGELRKLIEETGMDITVGLSEQKIRFDFDETRLTS
EEEEECCCCCCCCCCCCCEEECHHHHHHHHHHHHHCCCEEEEECCCCEEEECCHHHHHHH
KLIDGTFPDYERVIPTENDKVLEVDCKLFAQAADRVSAISTEKSRAIKVAVAKGTMTLSA
HHHCCCCCCHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECEEEEEC
SSPDAGSAVEEIEAAYQSTSLEIGFNSRYLLDILGQVEGGSVRLTFADAASPTVIRDVAD
CCCCCCHHHHHHHHHHHCCEEEECCCCEEEHHHHCCCCCCEEEEEEECCCCCHHHHHHCC
ASAVYVLMPMRV
CCEEEEEEECCC
>Mature Secondary Structure
MKLTIERTALLKSLGHVQSVVERRNTIPILSNVRMETGESGFSLNATDMDLEIVETVTAT
CEEEEHHHHHHHHHHHHHHHHHHCCCCCEEECCEEECCCCCCEECCCCCCHHHHHHHHHH
TLAPGATTAPAHTLYDIVRKLPDGSQVELSYDGNDGQLVLKAGRSRFSLSCLPVEDFPAL
EECCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEECCCEEEEEEECCCCCCCC
SAGEFSHSFSLPATDLRGLIDRTRFAISTEETRYYLNGIYLHATESAGVPVLRAVATDGH
CCCCCCCCCCCCHHHHHHHHHHHHEEEECCCCEEEEEEEEEEEECCCCCCEEEEECCCCC
RLARAEIPLPAGAAGMPGVIVPRKTVGELRKLIEETGMDITVGLSEQKIRFDFDETRLTS
EEEEECCCCCCCCCCCCCEEECHHHHHHHHHHHHHCCCEEEEECCCCEEEECCHHHHHHH
KLIDGTFPDYERVIPTENDKVLEVDCKLFAQAADRVSAISTEKSRAIKVAVAKGTMTLSA
HHHCCCCCCHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECEEEEEC
SSPDAGSAVEEIEAAYQSTSLEIGFNSRYLLDILGQVEGGSVRLTFADAASPTVIRDVAD
CCCCCCHHHHHHHHHHHCCEEEECCCCEEEHHHHCCCCCCEEEEEEECCCCCHHHHHHCC
ASAVYVLMPMRV
CCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9079919; 8226640 [H]