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The map label for this gene is mtbA [H]

Identifier: 83591166

GI number: 83591166

Start: 2467183

End: 2468232

Strand: Reverse

Name: mtbA [H]

Synonym: Moth_2346

Alternate gene names: 83591166

Gene position: 2468232-2467183 (Counterclockwise)

Preceding gene: 83591167

Following gene: 83591165

Centisome position: 93.89

GC content: 42.1

Gene sequence:

>1050_bases
ATGTTAAAGCAACGAGAATGGATGACACCGAAAAGGCGCTTCCTATCTGCGCTTTTCGGTGGAAGAGTTGATCGTACACC
GGTAGCGAATCCCACATCTTTGGTGACAGTAGAATTAATGGAGCGAACCGGTGCTTATTTTCCAGATGCCCATTTAGATG
CGGAAAAAATGGCTAGATTGGCGGCTACAAGTTACGAGGTATTAGGATTTGATACTATTATGCCAGTGTTTAGTGCCCAT
ACAGAATCAGCCGCCTTAGGAGTACCTGTCGATTGGGGAGATAAGATGAGTTGGCCTGTAAATACTTCTCACCCAATTAC
TGATCCGGAGCAGATAGTTATACCAGATAGCTTTCTAGAGGAGCCATCAATGCGTACTGTTTTGGATGCCATAAAGATTC
TAAGGAGTCAGTATGGAGATAGGGTGGCAATTATCGGCAAGACATATGGCCCATGGTCTCTAGCCTATCATCTGGTAGGA
ACGGAAAATTTTTTAATGGAAACAATTCTAAATCCGGACAAGGCAAGAAGATACCTCGAAGTATTATTGGAAGCCTCTTT
GCTTTCCGCCAAGGCACAAATTAAGGCTGGTGCAGATGCTATTTTATGGGGGGACCACGCTACTGGTGATCTGGTAAGCG
CAGAATATTATCGGGATTTTCTTATGAAAGTTCATCAGTATGTGACTAGGGAAGTCGGAGCTCCTATTATCCTGCATATT
TGTGGGAATACTACCAAATTCATTCCTTATATTGTAGAAGCCGGATTTGATGCTTTTCATTTTGATTCTAAAGTCGATGC
CAAACTAGCTAAAGAATTGGCTGGAAATAAGATGTCTTTAATTGGAAACATCAATAATCCTGTAACATTGCTCGCCGGCA
CACCTGAAGATGTTAAAAAAGAGACTCTATACGCAATTGAGGCTGGCGTGGAAATAGTCGGCCCAGAATGTGCTATTCCT
TTGACAACGCCCCTTGAAAACATTTTAGCTATTACTGAAACCGCAAAAGAATATCAAATTCATAAAAAACTAGGAGGGGA
GACACAATGA

Upstream 100 bases:

>100_bases
AGGGGGGTGGAGTATGAGGGCTGCATAATAACTTGCTCCAACCCGTACCGTGAGAGCAGATGAAAATCCTACAAAAAATA
TTAGATTAGGAGGAAAACCT

Downstream 100 bases:

>100_bases
ATAAGGAAAACAGTAACAGTAGCCTGATTACATGGTGGCGTTACCGGTACCTTATTGCGAGCATTCTATTTTTTGCATAT
AGCATCCAGTACCTGGATCG

Product: methyltransferase MtaA/CmuA

Products: CO2; Coproporphyrinogen III [C]

Alternate protein names: MT2-A; Methylcobamide:CoM methyltransferase II isozyme A [H]

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MLKQREWMTPKRRFLSALFGGRVDRTPVANPTSLVTVELMERTGAYFPDAHLDAEKMARLAATSYEVLGFDTIMPVFSAH
TESAALGVPVDWGDKMSWPVNTSHPITDPEQIVIPDSFLEEPSMRTVLDAIKILRSQYGDRVAIIGKTYGPWSLAYHLVG
TENFLMETILNPDKARRYLEVLLEASLLSAKAQIKAGADAILWGDHATGDLVSAEYYRDFLMKVHQYVTREVGAPIILHI
CGNTTKFIPYIVEAGFDAFHFDSKVDAKLAKELAGNKMSLIGNINNPVTLLAGTPEDVKKETLYAIEAGVEIVGPECAIP
LTTPLENILAITETAKEYQIHKKLGGETQ

Sequences:

>Translated_349_residues
MLKQREWMTPKRRFLSALFGGRVDRTPVANPTSLVTVELMERTGAYFPDAHLDAEKMARLAATSYEVLGFDTIMPVFSAH
TESAALGVPVDWGDKMSWPVNTSHPITDPEQIVIPDSFLEEPSMRTVLDAIKILRSQYGDRVAIIGKTYGPWSLAYHLVG
TENFLMETILNPDKARRYLEVLLEASLLSAKAQIKAGADAILWGDHATGDLVSAEYYRDFLMKVHQYVTREVGAPIILHI
CGNTTKFIPYIVEAGFDAFHFDSKVDAKLAKELAGNKMSLIGNINNPVTLLAGTPEDVKKETLYAIEAGVEIVGPECAIP
LTTPLENILAITETAKEYQIHKKLGGETQ
>Mature_349_residues
MLKQREWMTPKRRFLSALFGGRVDRTPVANPTSLVTVELMERTGAYFPDAHLDAEKMARLAATSYEVLGFDTIMPVFSAH
TESAALGVPVDWGDKMSWPVNTSHPITDPEQIVIPDSFLEEPSMRTVLDAIKILRSQYGDRVAIIGKTYGPWSLAYHLVG
TENFLMETILNPDKARRYLEVLLEASLLSAKAQIKAGADAILWGDHATGDLVSAEYYRDFLMKVHQYVTREVGAPIILHI
CGNTTKFIPYIVEAGFDAFHFDSKVDAKLAKELAGNKMSLIGNINNPVTLLAGTPEDVKKETLYAIEAGVEIVGPECAIP
LTTPLENILAITETAKEYQIHKKLGGETQ

Specific function: Catalyzes the transfer of the methyl group from the methylated corrinoid cofactor of mtmC, mtbC and mttC to coenzyme M [H]

COG id: COG0407

COG function: function code H; Uroporphyrinogen-III decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the uroporphyrinogen decarboxylase family. MtbA/mtaA subfamily [H]

Homologues:

Organism=Escherichia coli, GI2367337, Length=316, Percent_Identity=27.5316455696203, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006360
- InterPro:   IPR000257 [H]

Pfam domain/function: PF01208 URO-D [H]

EC number: 4.1.1.37 [C]

Molecular weight: Translated: 38578; Mature: 38578

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKQREWMTPKRRFLSALFGGRVDRTPVANPTSLVTVELMERTGAYFPDAHLDAEKMARL
CCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEHEEHHHCCCCCCCCCCCHHHHHHH
AATSYEVLGFDTIMPVFSAHTESAALGVPVDWGDKMSWPVNTSHPITDPEQIVIPDSFLE
HHCCHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCEEEECHHHHC
EPSMRTVLDAIKILRSQYGDRVAIIGKTYGPWSLAYHLVGTENFLMETILNPDKARRYLE
CCHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHEEEEECCHHHHHHHHCCHHHHHHHHH
VLLEASLLSAKAQIKAGADAILWGDHATGDLVSAEYYRDFLMKVHQYVTREVGAPIILHI
HHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
CGNTTKFIPYIVEAGFDAFHFDSKVDAKLAKELAGNKMSLIGNINNPVTLLAGTPEDVKK
CCCCHHHHHHHHHCCCCCEECCCHHHHHHHHHHCCCCEEEEECCCCCEEEEECCCHHHHH
ETLYAIEAGVEIVGPECAIPLTTPLENILAITETAKEYQIHKKLGGETQ
HHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLKQREWMTPKRRFLSALFGGRVDRTPVANPTSLVTVELMERTGAYFPDAHLDAEKMARL
CCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEHEEHHHCCCCCCCCCCCHHHHHHH
AATSYEVLGFDTIMPVFSAHTESAALGVPVDWGDKMSWPVNTSHPITDPEQIVIPDSFLE
HHCCHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCEEEECHHHHC
EPSMRTVLDAIKILRSQYGDRVAIIGKTYGPWSLAYHLVGTENFLMETILNPDKARRYLE
CCHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHEEEEECCHHHHHHHHCCHHHHHHHHH
VLLEASLLSAKAQIKAGADAILWGDHATGDLVSAEYYRDFLMKVHQYVTREVGAPIILHI
HHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
CGNTTKFIPYIVEAGFDAFHFDSKVDAKLAKELAGNKMSLIGNINNPVTLLAGTPEDVKK
CCCCHHHHHHHHHCCCCCEECCCHHHHHHHHHHCCCCEEEEECCCCCEEEEECCCHHHHH
ETLYAIEAGVEIVGPECAIPLTTPLENILAITETAKEYQIHKKLGGETQ
HHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Reducing agents [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.00085 {7-COOH-porphyrinogen} 0.00035 {7-COOH-porphyrinogen} 0.00231 {7-COOH,} 0.00533 {uroporphyrinogen} 0.00256 {uroporphyrinogen} 0.00133 {uroporphyrinogen} 0.001 {uroporphyrinogen} 0.0008 {uroporphyrinogen} 0.0004 {uroporphyrino

Substrates: H+; Uroporphyrinogen III [C]

Specific reaction: (4) H+ + Uroporphyrinogen III --> (4) CO2 + Coproporphyrinogen III [C]

General reaction: Decarboxylation [C]

Inhibitor: 5, 5'-Dithiobis (2-nitrobenzoate); 7-COOH-porphyrinogenI; 7-COOH-porphyrinogen III; Butanedione; Diethyl dicarbonate; Fe2+; Iodoacetamide; p-Hydroxy mercuribenzoate; Phenyl glyoxal; Pt2+; Sn2+; UroporphyrinogenI uroporphyrinogen III; Uroporphyrinogen III;

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]