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The map label for this gene is purA

Identifier: 83591130

GI number: 83591130

Start: 2428386

End: 2429669

Strand: Reverse

Name: purA

Synonym: Moth_2309

Alternate gene names: 83591130

Gene position: 2429669-2428386 (Counterclockwise)

Preceding gene: 83591131

Following gene: 83591129

Centisome position: 92.43

GC content: 61.84

Gene sequence:

>1284_bases
ATGGCAGCGGTAGTACTGGTTGGCGCCCAGTGGGGCGATGAAGGTAAGGGAAAAATCACAGACTACCTGGCTGAAAGGGC
CGATGTGGTGATTCGCTACCAGGGAGGTAGCAACGCCGGCCATACGGTAATGGTCGGCCATGAGGAATTTAAACTGCACC
TGGTGCCTTCGGGTATCCTCTACCCCGGCAAGCTCTGTATTATCGGTAACGGTGTAGTCCTCGACCCGGCGGTCCTGGTG
GAGGAGTTAGACGGCCTGGCGGCCCGGGGCGTGGATACCTCCGGTCTGAAGATCAGCAACCGGGCTCACCTGATCCTTCC
CTACCACAAAGGCCTGGACGCCGCCGAGGAGGAACACCGCGGTGCGGCCATGATTGGCACCACCAAAAGGGGTATCGGCC
CGGCCTATGTGGATAAAGCCGCCCGGACGGGTATCCGGGTGGGCGACCTCCTGGACTGGGAGGAGTTTAGCGCCAAAGTG
GCCCATAACCTGGCTGCCACCAATGAACTCCTGGCTAAGATTTATGACCGGCCGGGATATGATCTCCAGGCCATCCTGGA
GGAATACGCCGGTTACGCCCGGCGCCTGCGGCCGCTGATTGCCGACAGCGTTCGCCTGGTGAACCGGGCCCTCCAGGAGG
GGCGTAAGGTTCTCTTTGAAGGCGCCCAGGGGACCCTCCTGGACCTGGATCAGGGAACCTATCCCTTTGTGACTTCATCC
TATCCCGTTGCCGGCGGGGCCTGCATCGGCGCCGGCGTCGGCCCGACACGCATCGACAAGGTCATTGGCGTGGTCAAGGC
CTATACCACCAGGGTGGGTTCCGGCCCCTTCCCTACGGAGATTACCGGGCCTGCCGGTGACGCCCTGAGGCAACAGGGCA
TGGAATTCGGTACCACCACCGGGCGACCGCGCCGCTGCGGCTGGCTGGATACGGTTATCCTGCGCCATGCTGCCGAGGTA
AACGGCCTGACGGGTATCGCCCTGACCAAGCTGGACGTCCTGACGGGCCTTGATCCTTTAAGAATTTGTACCAGTTACCG
CTACCGGGGGACGGTGGGGGAAGATTTTCCGGCCAGCCTGAAGGCATTAGAGGAGTGCGAACCGGTTTATGAGGAACTCC
CGGGCTGGCACGAAGACATTACCGGCGCTAGGTCCCTGGATGACCTCCCGGCTAATTGCCGCCGTTATATCCGGCGGCTG
GAAGAGCTCACCGGCGTTCCCGTCCACCTCATCGCCGTGGGCCCGCGCCGGGACCAGACCATTGTTTTGGAGAGTCCTTT
TTAA

Upstream 100 bases:

>100_bases
CCTCGGCCGCCGGGGTGATTACCGCCCGGGCCATTATGGCGGGTACTTCCGCCCGTGACTAGCTGCAGTCGGTAAATTAA
AATCGAAGGGTGGTGCCTTG

Downstream 100 bases:

>100_bases
CCGTTTTTAATATTCCGTTTGAAGGGCGTTGGCCCTTCTTTTTTTATTTCCCGAAAAATACTTATTGAATTGCCCCAAAA
AAATGCTATAATAGAACCAT

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MAAVVLVGAQWGDEGKGKITDYLAERADVVIRYQGGSNAGHTVMVGHEEFKLHLVPSGILYPGKLCIIGNGVVLDPAVLV
EELDGLAARGVDTSGLKISNRAHLILPYHKGLDAAEEEHRGAAMIGTTKRGIGPAYVDKAARTGIRVGDLLDWEEFSAKV
AHNLAATNELLAKIYDRPGYDLQAILEEYAGYARRLRPLIADSVRLVNRALQEGRKVLFEGAQGTLLDLDQGTYPFVTSS
YPVAGGACIGAGVGPTRIDKVIGVVKAYTTRVGSGPFPTEITGPAGDALRQQGMEFGTTTGRPRRCGWLDTVILRHAAEV
NGLTGIALTKLDVLTGLDPLRICTSYRYRGTVGEDFPASLKALEECEPVYEELPGWHEDITGARSLDDLPANCRRYIRRL
EELTGVPVHLIAVGPRRDQTIVLESPF

Sequences:

>Translated_427_residues
MAAVVLVGAQWGDEGKGKITDYLAERADVVIRYQGGSNAGHTVMVGHEEFKLHLVPSGILYPGKLCIIGNGVVLDPAVLV
EELDGLAARGVDTSGLKISNRAHLILPYHKGLDAAEEEHRGAAMIGTTKRGIGPAYVDKAARTGIRVGDLLDWEEFSAKV
AHNLAATNELLAKIYDRPGYDLQAILEEYAGYARRLRPLIADSVRLVNRALQEGRKVLFEGAQGTLLDLDQGTYPFVTSS
YPVAGGACIGAGVGPTRIDKVIGVVKAYTTRVGSGPFPTEITGPAGDALRQQGMEFGTTTGRPRRCGWLDTVILRHAAEV
NGLTGIALTKLDVLTGLDPLRICTSYRYRGTVGEDFPASLKALEECEPVYEELPGWHEDITGARSLDDLPANCRRYIRRL
EELTGVPVHLIAVGPRRDQTIVLESPF
>Mature_426_residues
AAVVLVGAQWGDEGKGKITDYLAERADVVIRYQGGSNAGHTVMVGHEEFKLHLVPSGILYPGKLCIIGNGVVLDPAVLVE
ELDGLAARGVDTSGLKISNRAHLILPYHKGLDAAEEEHRGAAMIGTTKRGIGPAYVDKAARTGIRVGDLLDWEEFSAKVA
HNLAATNELLAKIYDRPGYDLQAILEEYAGYARRLRPLIADSVRLVNRALQEGRKVLFEGAQGTLLDLDQGTYPFVTSSY
PVAGGACIGAGVGPTRIDKVIGVVKAYTTRVGSGPFPTEITGPAGDALRQQGMEFGTTTGRPRRCGWLDTVILRHAAEVN
GLTGIALTKLDVLTGLDPLRICTSYRYRGTVGEDFPASLKALEECEPVYEELPGWHEDITGARSLDDLPANCRRYIRRLE
ELTGVPVHLIAVGPRRDQTIVLESPF

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=426, Percent_Identity=47.6525821596244, Blast_Score=369, Evalue=1e-102,
Organism=Homo sapiens, GI34577063, Length=428, Percent_Identity=44.1588785046729, Blast_Score=361, Evalue=1e-100,
Organism=Homo sapiens, GI40316944, Length=397, Percent_Identity=46.3476070528967, Blast_Score=328, Evalue=6e-90,
Organism=Escherichia coli, GI1790620, Length=426, Percent_Identity=50.4694835680751, Blast_Score=416, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI25146553, Length=426, Percent_Identity=44.131455399061, Blast_Score=347, Evalue=8e-96,
Organism=Caenorhabditis elegans, GI25146555, Length=426, Percent_Identity=44.131455399061, Blast_Score=346, Evalue=1e-95,
Organism=Saccharomyces cerevisiae, GI6324109, Length=429, Percent_Identity=42.4242424242424, Blast_Score=351, Evalue=1e-97,
Organism=Drosophila melanogaster, GI21356033, Length=421, Percent_Identity=44.8931116389549, Blast_Score=355, Evalue=3e-98,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_MOOTA (Q2RG43)

Other databases:

- EMBL:   CP000232
- RefSeq:   YP_431139.1
- ProteinModelPortal:   Q2RG43
- SMR:   Q2RG43
- STRING:   Q2RG43
- GeneID:   3831423
- GenomeReviews:   CP000232_GR
- KEGG:   mta:Moth_2309
- NMPDR:   fig|264732.9.peg.2346
- eggNOG:   COG0104
- HOGENOM:   HBG658237
- OMA:   DYVVRYQ
- PhylomeDB:   Q2RG43
- BioCyc:   MTHE264732:MOTH_2309-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 46072; Mature: 45941

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 128-128 BINDING 142-142 BINDING 223-223 BINDING 238-238 BINDING 302-302 BINDING 304-304

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAVVLVGAQWGDEGKGKITDYLAERADVVIRYQGGSNAGHTVMVGHEEFKLHLVPSGIL
CCEEEEEECCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCEEEEECCEEEEEECCCCCC
YPGKLCIIGNGVVLDPAVLVEELDGLAARGVDTSGLKISNRAHLILPYHKGLDAAEEEHR
CCCEEEEEECCEEECHHHHHHHHCCHHHCCCCCCCEEECCCEEEEEEECCCCCCHHHHCC
GAAMIGTTKRGIGPAYVDKAARTGIRVGDLLDWEEFSAKVAHNLAATNELLAKIYDRPGY
CEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DLQAILEEYAGYARRLRPLIADSVRLVNRALQEGRKVLFEGAQGTLLDLDQGTYPFVTSS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEECCCCCCCEEECC
YPVAGGACIGAGVGPTRIDKVIGVVKAYTTRVGSGPFPTEITGPAGDALRQQGMEFGTTT
CCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCCCCC
GRPRRCGWLDTVILRHAAEVNGLTGIALTKLDVLTGLDPLRICTSYRYRGTVGEDFPASL
CCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCHHH
KALEECEPVYEELPGWHEDITGARSLDDLPANCRRYIRRLEELTGVPVHLIAVGPRRDQT
HHHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCE
IVLESPF
EEEECCC
>Mature Secondary Structure 
AAVVLVGAQWGDEGKGKITDYLAERADVVIRYQGGSNAGHTVMVGHEEFKLHLVPSGIL
CEEEEEECCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCEEEEECCEEEEEECCCCCC
YPGKLCIIGNGVVLDPAVLVEELDGLAARGVDTSGLKISNRAHLILPYHKGLDAAEEEHR
CCCEEEEEECCEEECHHHHHHHHCCHHHCCCCCCCEEECCCEEEEEEECCCCCCHHHHCC
GAAMIGTTKRGIGPAYVDKAARTGIRVGDLLDWEEFSAKVAHNLAATNELLAKIYDRPGY
CEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DLQAILEEYAGYARRLRPLIADSVRLVNRALQEGRKVLFEGAQGTLLDLDQGTYPFVTSS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEECCCCCCCEEECC
YPVAGGACIGAGVGPTRIDKVIGVVKAYTTRVGSGPFPTEITGPAGDALRQQGMEFGTTT
CCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCCCCC
GRPRRCGWLDTVILRHAAEVNGLTGIALTKLDVLTGLDPLRICTSYRYRGTVGEDFPASL
CCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCHHH
KALEECEPVYEELPGWHEDITGARSLDDLPANCRRYIRRLEELTGVPVHLIAVGPRRDQT
HHHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCE
IVLESPF
EEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA