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The map label for this gene is glcF [H]

Identifier: 83591128

GI number: 83591128

Start: 2425495

End: 2426766

Strand: Reverse

Name: glcF [H]

Synonym: Moth_2307

Alternate gene names: 83591128

Gene position: 2426766-2425495 (Counterclockwise)

Preceding gene: 83591129

Following gene: 83591127

Centisome position: 92.32

GC content: 58.88

Gene sequence:

>1272_bases
GTGAGCTATTTAGATGACCTGGCCCTGGTTGGCGAAGAAATGGCCAAGTGCATGAAGTGCGGCAACTGCCAGGCAGTCTG
CCCTCTGTATAAAGAGATCCTGCGGGAAGCGGCTGTGGCCCGTGGTAAGGTCCAGCTGGCCTACGCCTACCTGCGGGGAG
AAATTCCGGCCACCCATTCCCTAGCTGAAAAGTTTCTCTTTTGCCTCACTTGTATGGCCTGCGTGGCCAACTGCCCCAGC
GGCGTCCGGGTGGACAAGGTAGTACTGGCGGCGCGGGCGGCCATGGTGCGGGAAAAAGGGCTACCCTGGTTAAAAAAGGT
TATCTTCCAGGGTTTAAAGCGGTCCTGGCTCTTCGATACGGCTTTGAAGACCGGTCGTCATTTCCAGTCCTTCGCCCTGC
GTTACCGGCCGGAAATCCAGCGCTGTAACCCCCGCTTTCCTATAGGACTGGAACTGAGACGGGTCCTGCCGCCCCTGGCC
AGGCGCACATTGAGAGAGGAATTCCCCGAGGTTATCCGCCCGGTGCAGCCCCGGGCCAGGGCAACCTTTTTCACAGGTTG
CCTGGAGAACTATATCTACACTGATATCGGCCGAGCAGTGGTCAACGTCCTCCTGGCCAACGATATCGAAGTTATAATCC
CCCGGGACCAACACTGCTGCGGTATACCCACCCTGGTCCACGGCGACGTCCTTACGGCCAGGGAGATGGCTGCTTCCAAC
CTGCATATTTTTCAGGGATATAACTTTGACTACCTGGTCACTGCCTGCGCTACCTGTGGCGAGGCCTGGAAGCAATACTA
TCCCAGCCTCATGGATAACGGCCCCCTGGGGGAGGTTGCCGGGCAGATGGCCAGAAAGGCCCGGGACATCAATGAATTCC
TGGTGGAGATTGATTACCGCGTGCCGACCGGCAGTCTGCCTTTAAAGGTGACTTACCACGATCCCTGCCACCTGGTGCGG
GGCCAGGGGATTAGCCGCCAGCCGCGGCAAATCCTGGGTTCCATCTCGGGGGTGGAAGTGGTGGAAATGAAGAATGCCGA
TCGCTGCTGCGGCAGTGCCGGTTCCTTCAGCCTGACTAATTACGATGTTTCCATGCAGGTCCAGGCCCACAAAGTGGCAG
CTATCAAGGCCACCGGCGCCGGTACGGTGGTCACCGCCTGCCCGGCCTGCCGCATGCAACTGGAAGACGGCCTGGCCCAG
GCCGGCCTGTCCCCCAGTGTCTTCCACGTAGTCCAACTTCTAGAACAGGCTTACGGTATTACCGCCAATTAG

Upstream 100 bases:

>100_bases
CCTGGACGTCATGCGCCGGATCAAGCAAGCCCTGGATCCCCTGGGGTTACTCAATCCCGGCAAACTCGTTGGTCCGGTCC
CAGCGAAGGAGTGAGGGGCC

Downstream 100 bases:

>100_bases
CTTCACGCCTCTATTTACGGGGAGGGTCATATTTGAAATCTCAGGAACTCATTACTTCCTTCACCAGCCAGGCCGAAGCC
ATGGGCGCCCGGGTGATCCA

Product: hypothetical protein

Products: FADH2; dihydroxy-acetone-phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MSYLDDLALVGEEMAKCMKCGNCQAVCPLYKEILREAAVARGKVQLAYAYLRGEIPATHSLAEKFLFCLTCMACVANCPS
GVRVDKVVLAARAAMVREKGLPWLKKVIFQGLKRSWLFDTALKTGRHFQSFALRYRPEIQRCNPRFPIGLELRRVLPPLA
RRTLREEFPEVIRPVQPRARATFFTGCLENYIYTDIGRAVVNVLLANDIEVIIPRDQHCCGIPTLVHGDVLTAREMAASN
LHIFQGYNFDYLVTACATCGEAWKQYYPSLMDNGPLGEVAGQMARKARDINEFLVEIDYRVPTGSLPLKVTYHDPCHLVR
GQGISRQPRQILGSISGVEVVEMKNADRCCGSAGSFSLTNYDVSMQVQAHKVAAIKATGAGTVVTACPACRMQLEDGLAQ
AGLSPSVFHVVQLLEQAYGITAN

Sequences:

>Translated_423_residues
MSYLDDLALVGEEMAKCMKCGNCQAVCPLYKEILREAAVARGKVQLAYAYLRGEIPATHSLAEKFLFCLTCMACVANCPS
GVRVDKVVLAARAAMVREKGLPWLKKVIFQGLKRSWLFDTALKTGRHFQSFALRYRPEIQRCNPRFPIGLELRRVLPPLA
RRTLREEFPEVIRPVQPRARATFFTGCLENYIYTDIGRAVVNVLLANDIEVIIPRDQHCCGIPTLVHGDVLTAREMAASN
LHIFQGYNFDYLVTACATCGEAWKQYYPSLMDNGPLGEVAGQMARKARDINEFLVEIDYRVPTGSLPLKVTYHDPCHLVR
GQGISRQPRQILGSISGVEVVEMKNADRCCGSAGSFSLTNYDVSMQVQAHKVAAIKATGAGTVVTACPACRMQLEDGLAQ
AGLSPSVFHVVQLLEQAYGITAN
>Mature_422_residues
SYLDDLALVGEEMAKCMKCGNCQAVCPLYKEILREAAVARGKVQLAYAYLRGEIPATHSLAEKFLFCLTCMACVANCPSG
VRVDKVVLAARAAMVREKGLPWLKKVIFQGLKRSWLFDTALKTGRHFQSFALRYRPEIQRCNPRFPIGLELRRVLPPLAR
RTLREEFPEVIRPVQPRARATFFTGCLENYIYTDIGRAVVNVLLANDIEVIIPRDQHCCGIPTLVHGDVLTAREMAASNL
HIFQGYNFDYLVTACATCGEAWKQYYPSLMDNGPLGEVAGQMARKARDINEFLVEIDYRVPTGSLPLKVTYHDPCHLVRG
QGISRQPRQILGSISGVEVVEMKNADRCCGSAGSFSLTNYDVSMQVQAHKVAAIKATGAGTVVTACPACRMQLEDGLAQA
GLSPSVFHVVQLLEQAYGITAN

Specific function: Electron Transfer Protein; May Also Function As The Membrane Anchor For The Glpab Dimer. [C]

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Loosely Bound To The Cytoplasm Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1788576, Length=413, Percent_Identity=27.1186440677966, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI48994913, Length=406, Percent_Identity=25.3694581280788, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1786497, Length=234, Percent_Identity=26.4957264957265, Blast_Score=92, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: NA

Molecular weight: Translated: 46751; Mature: 46620

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
4.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYLDDLALVGEEMAKCMKCGNCQAVCPLYKEILREAAVARGKVQLAYAYLRGEIPATHS
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHCCCCCHHH
LAEKFLFCLTCMACVANCPSGVRVDKVVLAARAAMVREKGLPWLKKVIFQGLKRSWLFDT
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
ALKTGRHFQSFALRYRPEIQRCNPRFPIGLELRRVLPPLARRTLREEFPEVIRPVQPRAR
HHHCCCHHHHHHHHHCCCHHCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHH
ATFFTGCLENYIYTDIGRAVVNVLLANDIEVIIPRDQHCCGIPTLVHGDVLTAREMAASN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHCCCHHHHHHHHHCC
LHIFQGYNFDYLVTACATCGEAWKQYYPSLMDNGPLGEVAGQMARKARDINEFLVEIDYR
EEEEECCCHHHHHHHHHHHHHHHHHHCHHHHCCCCHHHHHHHHHHHHHHHHHHHHEECCC
VPTGSLPLKVTYHDPCHLVRGQGISRQPRQILGSISGVEVVEMKNADRCCGSAGSFSLTN
CCCCCCCEEEEECCCHHHHCCCCCCCCHHHHHCCCCCEEEEEECCCHHHCCCCCCCEEEC
YDVSMQVQAHKVAAIKATGAGTVVTACPACRMQLEDGLAQAGLSPSVFHVVQLLEQAYGI
CEEEEEEEEEEEEEEEECCCCCHHHHCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
TAN
CCC
>Mature Secondary Structure 
SYLDDLALVGEEMAKCMKCGNCQAVCPLYKEILREAAVARGKVQLAYAYLRGEIPATHS
CHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHCCCCCHHH
LAEKFLFCLTCMACVANCPSGVRVDKVVLAARAAMVREKGLPWLKKVIFQGLKRSWLFDT
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
ALKTGRHFQSFALRYRPEIQRCNPRFPIGLELRRVLPPLARRTLREEFPEVIRPVQPRAR
HHHCCCHHHHHHHHHCCCHHCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHH
ATFFTGCLENYIYTDIGRAVVNVLLANDIEVIIPRDQHCCGIPTLVHGDVLTAREMAASN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHCCCHHHHHHHHHCC
LHIFQGYNFDYLVTACATCGEAWKQYYPSLMDNGPLGEVAGQMARKARDINEFLVEIDYR
EEEEECCCHHHHHHHHHHHHHHHHHHCHHHHCCCCHHHHHHHHHHHHHHHHHHHHEECCC
VPTGSLPLKVTYHDPCHLVRGQGISRQPRQILGSISGVEVVEMKNADRCCGSAGSFSLTN
CCCCCCCEEEEECCCHHHHCCCCCCCCHHHHHCCCCCEEEEEECCCHHHCCCCCCCEEEC
YDVSMQVQAHKVAAIKATGAGTVVTACPACRMQLEDGLAQAGLSPSVFHVVQLLEQAYGI
CEEEEEEEEEEEEEEEECCCCCHHHHCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
TAN
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: FAD; glycerol-3-phosphate [C]

Specific reaction: FAD + glycerol-3-phosphate = FADH2 + dihydroxy-acetone-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]