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The map label for this gene is ilvB [H]

Identifier: 83591079

GI number: 83591079

Start: 2363017

End: 2364726

Strand: Reverse

Name: ilvB [H]

Synonym: Moth_2258

Alternate gene names: 83591079

Gene position: 2364726-2363017 (Counterclockwise)

Preceding gene: 83591080

Following gene: 83591078

Centisome position: 89.96

GC content: 56.26

Gene sequence:

>1710_bases
GTGCTTTTTACCATTGGGGATCGAGCTGAAGGCAAAAAACTAACCGGAGCGGAGATTATTGTTCAGGCCCTCCAGGCAGA
GGGAGTGGATACCGTATTTGGTATCCCGGGGGGCTCCGTCCTGCCCCTTTATCATGAGCTGGCGGTAGCCAGCATACGCC
ATATTTTAACGCGGCATGAGCAGGCAGCCGCCCATGCCGCCGACGGTTATGCCCGCGCCAGCGGCAAACCCGGTGTGTGT
ATTGCTACTTCCGGCCCTGGAGCAACCAACCTGGTGACGGGGATCGCCAATGCCTATATGGATTCCATCCCCCTGGTTGC
CATTACCGGCCAGGTCAGTGTTCCTATGATTGGCCATGATTCCTTCCAGGAAGCCGACATTACCGGCATTACCCTCCCCA
TTACCAAGGCCAATTACCTGGTCAAGAACGTCCACGACCTGGCAGCTACCATCCACGAGGCTTTTTACATCGCTACCACC
GGCCGTCCGGGTCCGGTGTTGATCGATATTCCCAAGGATATTACCACCCAGCGGGCACTGTTTCGCTACCCTCCCAGACT
CCACCTTCCAGGTTACCGGTGCAAGGTGCAACCCCACGCCCTCCAGGTAGCCCAGGCGGCTGCCGCTATCGGGGCTGCGG
AAAAACCTTTACTCTTTATTGGTGGCGGCGTAATCACCTCAGAAGCCCATGAAGAAGTACGGCAACTGGCTGAGGGCCAG
GATATTCCCGTAGTCATGAGCATGATGGGCAAGGGCGGTTTCCCGGAAACCCATCCTTTATTTGTAGGCATGGTGGGCAT
GCATGGTACAGCGGCAGCCAACTACGCCATGTGTGAGACGGATCTCATTATCGGTGTGGGTGTCCGTTTTGACGACCGGG
TCACGGGTAAAGTCGAAGCCTTTGCTCCGAAAGCTAAAATTATCCACATCGATATTGATGCCGCTGAGATCGGCAAGGTG
GTTCAGGCCCATATTCCCATTGTCAGCGATGCTCGTCAGGGGTTGGCCGCCATCCTGGAGAAATTGTCGGGAAAAGGCGA
TCACCAGGCCTGGCGGCAGCAAATCCGGCGCTGGCAGGAAGAAAATCCGTTACGCTATGAAAAGAGTGGCCTGAAACCCC
AGTATGTCCTGGAGGAATTATATAATCTTACTAAGGGGCAGGCCATTATCTGCACTGATGTCGGCCAGCACCAGATGTGG
GCGGTGCAGTATTATCCCCTGAGCCGGCCCCGCGCCTTCCTTTCGTCCTGCGGATTAGGAACCATGGGCTTTGGCGTCCC
GGCAGCCATGGGAGCCGCTGTTGCCCGGCCGGGGGAGCCGATTGTGGTTATTACTGGCGACGGTAGTTTCCAGATGAATA
TCCAGGAGCTCGCTACCATTAGCCATTACCAGTTGCCACTCAAGATTATTATCATGAACAACGGTTACCTGGGTATGGTG
CGTCAGTGGCAGGAATTCTTTTTTAACCGCCGCTATGCCTACTCGGAGATGCTCGGTAATCCGGACTTTGTCAAGGTGGC
TGAGGCCTACCGCATTCCGGGGCGGCTGGTTAACGAAAGCCGGGAGGTTGTTCCCGCTCTGGAAGAAGCCCTGGCTGCAG
AAGGCCCCTTCCTGGTTGATGTCAGGATCGACAGGGAGGAGAACGTCTTTCCCATGGTGCCGCCGGGGGGCACCCTCAAT
AAGATGGTGACGGGAGGTAAGGGAGCATGA

Upstream 100 bases:

>100_bases
CGCCGAAGATCACCGGCGGTTACCTGGGCCGCTACGCGCGCATGGTTACTTCCGGAGCCAGGGGCGCGGTGTTGGAGTAA
AATATTTTAAGCGCAAAGGG

Downstream 100 bases:

>100_bases
AGCGCACCCTTTCAGTCCTGGTGGAAAACCGTCCCGGCGTCCTGACCCGGGTGGCCGGTCTTTTCAGTCGCCGGGGTTAT
AACATTGACAGCCTGGCTGT

Product: acetolactate synthase, large subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS; Vegetative protein 105; VEG105 [H]

Number of amino acids: Translated: 569; Mature: 569

Protein sequence:

>569_residues
MLFTIGDRAEGKKLTGAEIIVQALQAEGVDTVFGIPGGSVLPLYHELAVASIRHILTRHEQAAAHAADGYARASGKPGVC
IATSGPGATNLVTGIANAYMDSIPLVAITGQVSVPMIGHDSFQEADITGITLPITKANYLVKNVHDLAATIHEAFYIATT
GRPGPVLIDIPKDITTQRALFRYPPRLHLPGYRCKVQPHALQVAQAAAAIGAAEKPLLFIGGGVITSEAHEEVRQLAEGQ
DIPVVMSMMGKGGFPETHPLFVGMVGMHGTAAANYAMCETDLIIGVGVRFDDRVTGKVEAFAPKAKIIHIDIDAAEIGKV
VQAHIPIVSDARQGLAAILEKLSGKGDHQAWRQQIRRWQEENPLRYEKSGLKPQYVLEELYNLTKGQAIICTDVGQHQMW
AVQYYPLSRPRAFLSSCGLGTMGFGVPAAMGAAVARPGEPIVVITGDGSFQMNIQELATISHYQLPLKIIIMNNGYLGMV
RQWQEFFFNRRYAYSEMLGNPDFVKVAEAYRIPGRLVNESREVVPALEEALAAEGPFLVDVRIDREENVFPMVPPGGTLN
KMVTGGKGA

Sequences:

>Translated_569_residues
MLFTIGDRAEGKKLTGAEIIVQALQAEGVDTVFGIPGGSVLPLYHELAVASIRHILTRHEQAAAHAADGYARASGKPGVC
IATSGPGATNLVTGIANAYMDSIPLVAITGQVSVPMIGHDSFQEADITGITLPITKANYLVKNVHDLAATIHEAFYIATT
GRPGPVLIDIPKDITTQRALFRYPPRLHLPGYRCKVQPHALQVAQAAAAIGAAEKPLLFIGGGVITSEAHEEVRQLAEGQ
DIPVVMSMMGKGGFPETHPLFVGMVGMHGTAAANYAMCETDLIIGVGVRFDDRVTGKVEAFAPKAKIIHIDIDAAEIGKV
VQAHIPIVSDARQGLAAILEKLSGKGDHQAWRQQIRRWQEENPLRYEKSGLKPQYVLEELYNLTKGQAIICTDVGQHQMW
AVQYYPLSRPRAFLSSCGLGTMGFGVPAAMGAAVARPGEPIVVITGDGSFQMNIQELATISHYQLPLKIIIMNNGYLGMV
RQWQEFFFNRRYAYSEMLGNPDFVKVAEAYRIPGRLVNESREVVPALEEALAAEGPFLVDVRIDREENVFPMVPPGGTLN
KMVTGGKGA
>Mature_569_residues
MLFTIGDRAEGKKLTGAEIIVQALQAEGVDTVFGIPGGSVLPLYHELAVASIRHILTRHEQAAAHAADGYARASGKPGVC
IATSGPGATNLVTGIANAYMDSIPLVAITGQVSVPMIGHDSFQEADITGITLPITKANYLVKNVHDLAATIHEAFYIATT
GRPGPVLIDIPKDITTQRALFRYPPRLHLPGYRCKVQPHALQVAQAAAAIGAAEKPLLFIGGGVITSEAHEEVRQLAEGQ
DIPVVMSMMGKGGFPETHPLFVGMVGMHGTAAANYAMCETDLIIGVGVRFDDRVTGKVEAFAPKAKIIHIDIDAAEIGKV
VQAHIPIVSDARQGLAAILEKLSGKGDHQAWRQQIRRWQEENPLRYEKSGLKPQYVLEELYNLTKGQAIICTDVGQHQMW
AVQYYPLSRPRAFLSSCGLGTMGFGVPAAMGAAVARPGEPIVVITGDGSFQMNIQELATISHYQLPLKIIIMNNGYLGMV
RQWQEFFFNRRYAYSEMLGNPDFVKVAEAYRIPGRLVNESREVVPALEEALAAEGPFLVDVRIDREENVFPMVPPGGTLN
KMVTGGKGA

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=580, Percent_Identity=28.1034482758621, Blast_Score=185, Evalue=9e-47,
Organism=Homo sapiens, GI21361361, Length=581, Percent_Identity=25.1290877796902, Blast_Score=153, Evalue=5e-37,
Organism=Escherichia coli, GI87081685, Length=563, Percent_Identity=47.4245115452931, Blast_Score=537, Evalue=1e-154,
Organism=Escherichia coli, GI1790104, Length=561, Percent_Identity=47.0588235294118, Blast_Score=499, Evalue=1e-142,
Organism=Escherichia coli, GI1786717, Length=562, Percent_Identity=33.4519572953737, Blast_Score=306, Evalue=2e-84,
Organism=Escherichia coli, GI1787096, Length=549, Percent_Identity=28.0510018214936, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI1788716, Length=544, Percent_Identity=27.3897058823529, Blast_Score=183, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI17531299, Length=576, Percent_Identity=26.9097222222222, Blast_Score=171, Evalue=9e-43,
Organism=Caenorhabditis elegans, GI17531301, Length=576, Percent_Identity=26.9097222222222, Blast_Score=171, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17542570, Length=602, Percent_Identity=25.0830564784053, Blast_Score=147, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6323755, Length=578, Percent_Identity=42.2145328719723, Blast_Score=456, Evalue=1e-129,
Organism=Saccharomyces cerevisiae, GI6320816, Length=481, Percent_Identity=27.027027027027, Blast_Score=118, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6323073, Length=503, Percent_Identity=22.8628230616302, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6323163, Length=484, Percent_Identity=24.1735537190083, Blast_Score=101, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6321524, Length=506, Percent_Identity=22.3320158102767, Blast_Score=94, Evalue=5e-20,
Organism=Drosophila melanogaster, GI19922626, Length=492, Percent_Identity=28.2520325203252, Blast_Score=176, Evalue=4e-44,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 61395; Mature: 61395

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFTIGDRAEGKKLTGAEIIVQALQAEGVDTVFGIPGGSVLPLYHELAVASIRHILTRHE
CEEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
QAAAHAADGYARASGKPGVCIATSGPGATNLVTGIANAYMDSIPLVAITGQVSVPMIGHD
HHHHHHCCCHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCEEEEEECCC
SFQEADITGITLPITKANYLVKNVHDLAATIHEAFYIATTGRPGPVLIDIPKDITTQRAL
CCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHEEEEEECCCCCCEEEECCCCCHHHHHH
FRYPPRLHLPGYRCKVQPHALQVAQAAAAIGAAEKPLLFIGGGVITSEAHEEVRQLAEGQ
HCCCCCCCCCCCEEEECHHHHHHHHHHHHHCCCCCCEEEECCCEECHHHHHHHHHHHCCC
DIPVVMSMMGKGGFPETHPLFVGMVGMHGTAAANYAMCETDLIIGVGVRFDDRVTGKVEA
CCHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCEEEEEEEEEEEECCEECCCCCCEEEE
FAPKAKIIHIDIDAAEIGKVVQAHIPIVSDARQGLAAILEKLSGKGDHQAWRQQIRRWQE
ECCCEEEEEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
ENPLRYEKSGLKPQYVLEELYNLTKGQAIICTDVGQHQMWAVQYYPLSRPRAFLSSCGLG
CCCCEEHHCCCCHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEECCCCHHHHHHHCCCC
TMGFGVPAAMGAAVARPGEPIVVITGDGSFQMNIQELATISHYQLPLKIIIMNNGYLGMV
CCCCCCHHHHHHHHCCCCCCEEEEECCCCEEEEHHHHHHHHEEECCEEEEEECCCCHHHH
RQWQEFFFNRRYAYSEMLGNPDFVKVAEAYRIPGRLVNESREVVPALEEALAAEGPFLVD
HHHHHHHHCCHHHHHHHCCCCCCEEHHHHHCCCCHHHCCCHHHHHHHHHHHHCCCCEEEE
VRIDREENVFPMVPPGGTLNKMVTGGKGA
EEECCCCCEEEECCCCCCHHHHCCCCCCC
>Mature Secondary Structure
MLFTIGDRAEGKKLTGAEIIVQALQAEGVDTVFGIPGGSVLPLYHELAVASIRHILTRHE
CEEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
QAAAHAADGYARASGKPGVCIATSGPGATNLVTGIANAYMDSIPLVAITGQVSVPMIGHD
HHHHHHCCCHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCEEEEEECCC
SFQEADITGITLPITKANYLVKNVHDLAATIHEAFYIATTGRPGPVLIDIPKDITTQRAL
CCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHEEEEEECCCCCCEEEECCCCCHHHHHH
FRYPPRLHLPGYRCKVQPHALQVAQAAAAIGAAEKPLLFIGGGVITSEAHEEVRQLAEGQ
HCCCCCCCCCCCEEEECHHHHHHHHHHHHHCCCCCCEEEECCCEECHHHHHHHHHHHCCC
DIPVVMSMMGKGGFPETHPLFVGMVGMHGTAAANYAMCETDLIIGVGVRFDDRVTGKVEA
CCHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCEEEEEEEEEEEECCEECCCCCCEEEE
FAPKAKIIHIDIDAAEIGKVVQAHIPIVSDARQGLAAILEKLSGKGDHQAWRQQIRRWQE
ECCCEEEEEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
ENPLRYEKSGLKPQYVLEELYNLTKGQAIICTDVGQHQMWAVQYYPLSRPRAFLSSCGLG
CCCCEEHHCCCCHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEECCCCHHHHHHHCCCC
TMGFGVPAAMGAAVARPGEPIVVITGDGSFQMNIQELATISHYQLPLKIIIMNNGYLGMV
CCCCCCHHHHHHHHCCCCCCEEEEECCCCEEEEHHHHHHHHEEECCEEEEEECCCCHHHH
RQWQEFFFNRRYAYSEMLGNPDFVKVAEAYRIPGRLVNESREVVPALEEALAAEGPFLVD
HHHHHHHHCCHHHHHHHCCCCCCEEHHHHHCCCCHHHCCCHHHHHHHHHHHHCCCCEEEE
VRIDREENVFPMVPPGGTLNKMVTGGKGA
EEECCCCCEEEECCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377; 1577690; 9298659 [H]