| Definition | Moorella thermoacetica ATCC 39073, complete genome. |
|---|---|
| Accession | NC_007644 |
| Length | 2,628,784 |
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The map label for this gene is hypF [H]
Identifier: 83591002
GI number: 83591002
Start: 2278031
End: 2280334
Strand: Reverse
Name: hypF [H]
Synonym: Moth_2179
Alternate gene names: 83591002
Gene position: 2280334-2278031 (Counterclockwise)
Preceding gene: 83591003
Following gene: 83591001
Centisome position: 86.74
GC content: 55.34
Gene sequence:
>2304_bases ATGGCTCAAAGAAGCAATGCAGAGAAAAAGGGGATTGTCCGCTATCGGATAATCGTCAAAGGGATCGTCCAGGGCGTAGG GTTTCGCCCCTTTATTTATAACCTGGCGAGGCTCTATCGCCTGAAGGGCACTGTTGCCAATACCAGCCAGGGCGTGCTTA TCGAAGCCGAAGGCCAGGAGGAGACAGTACACGCTTTTCTGGCCAGTTTAAAGGAAAAGCATCCCCCGTTATCGCGAATA ACAGCTTTAGAATGGGACGTCCTGGAACCCTGTGGCTATAGTTCCTTTGCTATTATCCCCAGTGGTGAAGGGCTCGGAAA AGAAGCCTTGATTCCCCCTGATGTAGCCCTGTGTGCTGATTGTGCCCGGGAAATAATGGACCCCCGGGACAGGCATTACG GCTACCCCTTTACCAACTGTACCAATTGTGGACCGCGATTTACCATTGTACGGGGCGTACCTTATGACCGTGCGAAAACC TCCATGGCCCGGTTTCCGATGTGTCCGGAATGCGCCCGGGAATACCACGATCCGGGCAACAGGAGATTCCACGCCCAGCC GGCCGCCTGCCCGGCCTGCGGGCCGCAGGTGGAGCTGGTGGACAGGCAGGGGCGAAAGGTGGAAGGCAACTGGTTGGAGC TGAGCTGGAGATTCTTGCAAGACGGCAAGATATTAGCCGTAAAAGGGCTGGGAGGCTTTCACCTGGTATGCGATGCCAAG AATAGGGCAGCTTTAAAGACCCTCCGCCGGCGCAAGGGCCGTGAAGCCAAACCCCTGGCGGTAATGTGCCTCCTGGAGAC GGCCAGGAAGTACTGTTACGTTGGTCCGGAGGAAGAAAAACTCCTGTCTTCTCCTCAAGCACCTATTGTTATCCTTACCA AGCGGGCCGACTGTAATTTGCCGGATGAGCTGGCCCCGGGGATGAAGACCCTGGGTATAATGTTACCTTATACACCGCTG CACCTGATGCTTTTAAACGGCCCCCTGGAAATTTTAGTCATGACCAGCGGTAATCGCAACGGTTTGCCCCTGGCAAAAGA CAACGGGAGAGCCCTGGAGGAACTGGGCGGTATAGCCGACTATTTTCTCTGGCACAATCGCGAGATTGTCAACCGCTGCG ACGATTCCGTCGTAGCGGTGATAGGTGATACGGCTCAGATTTTGCGCCGCTCGCGGGGATATGTCCCCTCGCCCGTTAAG GTTGCGGTTAAATCCAGTTCCCCTGTGCTGGGCGCCGGTGGGGACATGAAAAACACCTTTTGCCTTTTAAAAGGCAATCA AGCCTTTGTCAGCCAGCACATCGGCGACCTGGGCAGCAGGGAAGGTGAAGCACACTTTTTCGCCAGCCTGGAGAATTTAA AAAACCTCATCGGCTCCGAACCTGAAGTGGTCGGTTATGACATGCATCCCGGCTATCGTTCCTCCCGCCTGGCGGCAGGA ATTCCGGCCAAGGCACACTTTGCCGTTCAACACCATCACGCCCATATGGTTTCTTGCCTGGCCGACAATGGCGTGGATGA GGATGCAATCGGGGTAATCCTTGATGGAACCGGATACGGAACCGACGGACGTCTCTGGGGTTTTGAGATCCTTACCGGGG ATTGTGCCGATTTTACCAGGGAGTATCACCTGGCCTATGTACCGTTGCCCGGTGGCGAGCAGGCAGTACGTTACCCATGG CGGACAGCAGTAGCCTATTTAATGAAATACCTTTCGGCGCAAGGCGAGTCGCTTGCCGACCGCCTTTTTCAAAGCAGGGG GCAGGAGCTTGAGGTTATCAAACGTCTCGTCGCCACAGGCTTTAATTCACCTTTAAGCTCGAGCTGCGGTCGTCTTTTTG ACGCCGTATCGGCCCTCCTCGGCCTCTGCTACCATAATAGTTACGAAGGACAGGCGGCAATCGAGCTAGGAGAAATGGTC CTGGACCCGGCGGAGGGCAAAAGATTAATACCCTATCCTTTTTTTATCGAGGGGAAAGTTATCCATCCGGGCGGGGTCAT AGCCGGCGTGGCGGCCGACCTGGAACGGGGAGTTGCCAGGGAGATTATTGCCACCCGTTTCCACAATACGGTCCTGGCGA TGGTACGCGAGGCGGTACGCCGGGTTGCTGAAAGAACACATATAAAAACTGTAGCCCTTAGCGGTGGTGCCTGGCAAAAC CGCTATCTTTTCAGCCTTGCTAAAGAAATCTTGCCTGGCGACGGTTATCGCCTGCTGGTCCACCGGCAGGTACCGGCCAA TGATGGGGGGCTTTCCCTGGGCCAGGCAGTAATCGCTTGCCGGAGGTGGCAACAATGTGTTTAG
Upstream 100 bases:
>100_bases ACTTGAAAGCAGTCGAAGAAGAGATTAAGGCAGCCAATCCTGCTGCGAAGTTTTTCGTAACCTCAGCTAAAACCGGCGAG GGCCTGGAGGCTTGGTGTTT
Downstream 100 bases:
>100_bases GTATTGTGGGTAAAGTAATTAAGGTATCTGCGGCTGATGAAACCGCGATAATTGATGTTCACGGCGCCCGCAGGGAGATA TCCATAGCTTTACTGGGGCA
Product: (NiFe) hydrogenase maturation protein HypF
Products: NA
Alternate protein names: Carbamoyl phosphate-converting enzyme hypF; [NiFe]-hydrogenase maturation factor hypF; Hydrogenase maturation protein hypF [H]
Number of amino acids: Translated: 767; Mature: 766
Protein sequence:
>767_residues MAQRSNAEKKGIVRYRIIVKGIVQGVGFRPFIYNLARLYRLKGTVANTSQGVLIEAEGQEETVHAFLASLKEKHPPLSRI TALEWDVLEPCGYSSFAIIPSGEGLGKEALIPPDVALCADCAREIMDPRDRHYGYPFTNCTNCGPRFTIVRGVPYDRAKT SMARFPMCPECAREYHDPGNRRFHAQPAACPACGPQVELVDRQGRKVEGNWLELSWRFLQDGKILAVKGLGGFHLVCDAK NRAALKTLRRRKGREAKPLAVMCLLETARKYCYVGPEEEKLLSSPQAPIVILTKRADCNLPDELAPGMKTLGIMLPYTPL HLMLLNGPLEILVMTSGNRNGLPLAKDNGRALEELGGIADYFLWHNREIVNRCDDSVVAVIGDTAQILRRSRGYVPSPVK VAVKSSSPVLGAGGDMKNTFCLLKGNQAFVSQHIGDLGSREGEAHFFASLENLKNLIGSEPEVVGYDMHPGYRSSRLAAG IPAKAHFAVQHHHAHMVSCLADNGVDEDAIGVILDGTGYGTDGRLWGFEILTGDCADFTREYHLAYVPLPGGEQAVRYPW RTAVAYLMKYLSAQGESLADRLFQSRGQELEVIKRLVATGFNSPLSSSCGRLFDAVSALLGLCYHNSYEGQAAIELGEMV LDPAEGKRLIPYPFFIEGKVIHPGGVIAGVAADLERGVAREIIATRFHNTVLAMVREAVRRVAERTHIKTVALSGGAWQN RYLFSLAKEILPGDGYRLLVHRQVPANDGGLSLGQAVIACRRWQQCV
Sequences:
>Translated_767_residues MAQRSNAEKKGIVRYRIIVKGIVQGVGFRPFIYNLARLYRLKGTVANTSQGVLIEAEGQEETVHAFLASLKEKHPPLSRI TALEWDVLEPCGYSSFAIIPSGEGLGKEALIPPDVALCADCAREIMDPRDRHYGYPFTNCTNCGPRFTIVRGVPYDRAKT SMARFPMCPECAREYHDPGNRRFHAQPAACPACGPQVELVDRQGRKVEGNWLELSWRFLQDGKILAVKGLGGFHLVCDAK NRAALKTLRRRKGREAKPLAVMCLLETARKYCYVGPEEEKLLSSPQAPIVILTKRADCNLPDELAPGMKTLGIMLPYTPL HLMLLNGPLEILVMTSGNRNGLPLAKDNGRALEELGGIADYFLWHNREIVNRCDDSVVAVIGDTAQILRRSRGYVPSPVK VAVKSSSPVLGAGGDMKNTFCLLKGNQAFVSQHIGDLGSREGEAHFFASLENLKNLIGSEPEVVGYDMHPGYRSSRLAAG IPAKAHFAVQHHHAHMVSCLADNGVDEDAIGVILDGTGYGTDGRLWGFEILTGDCADFTREYHLAYVPLPGGEQAVRYPW RTAVAYLMKYLSAQGESLADRLFQSRGQELEVIKRLVATGFNSPLSSSCGRLFDAVSALLGLCYHNSYEGQAAIELGEMV LDPAEGKRLIPYPFFIEGKVIHPGGVIAGVAADLERGVAREIIATRFHNTVLAMVREAVRRVAERTHIKTVALSGGAWQN RYLFSLAKEILPGDGYRLLVHRQVPANDGGLSLGQAVIACRRWQQCV >Mature_766_residues AQRSNAEKKGIVRYRIIVKGIVQGVGFRPFIYNLARLYRLKGTVANTSQGVLIEAEGQEETVHAFLASLKEKHPPLSRIT ALEWDVLEPCGYSSFAIIPSGEGLGKEALIPPDVALCADCAREIMDPRDRHYGYPFTNCTNCGPRFTIVRGVPYDRAKTS MARFPMCPECAREYHDPGNRRFHAQPAACPACGPQVELVDRQGRKVEGNWLELSWRFLQDGKILAVKGLGGFHLVCDAKN RAALKTLRRRKGREAKPLAVMCLLETARKYCYVGPEEEKLLSSPQAPIVILTKRADCNLPDELAPGMKTLGIMLPYTPLH LMLLNGPLEILVMTSGNRNGLPLAKDNGRALEELGGIADYFLWHNREIVNRCDDSVVAVIGDTAQILRRSRGYVPSPVKV AVKSSSPVLGAGGDMKNTFCLLKGNQAFVSQHIGDLGSREGEAHFFASLENLKNLIGSEPEVVGYDMHPGYRSSRLAAGI PAKAHFAVQHHHAHMVSCLADNGVDEDAIGVILDGTGYGTDGRLWGFEILTGDCADFTREYHLAYVPLPGGEQAVRYPWR TAVAYLMKYLSAQGESLADRLFQSRGQELEVIKRLVATGFNSPLSSSCGRLFDAVSALLGLCYHNSYEGQAAIELGEMVL DPAEGKRLIPYPFFIEGKVIHPGGVIAGVAADLERGVAREIIATRFHNTVLAMVREAVRRVAERTHIKTVALSGGAWQNR YLFSLAKEILPGDGYRLLVHRQVPANDGGLSLGQAVIACRRWQQCV
Specific function: Along with hypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases using carbamoylphosphate as a substrate. It functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carb
COG id: COG0068
COG function: function code O; Hydrogenase maturation factor
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 YrdC-like domain [H]
Homologues:
Organism=Escherichia coli, GI2367152, Length=767, Percent_Identity=40.8083441981747, Blast_Score=462, Evalue=1e-131,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001792 - InterPro: IPR017968 - InterPro: IPR004421 - InterPro: IPR017945 - InterPro: IPR006070 - InterPro: IPR011125 [H]
Pfam domain/function: PF00708 Acylphosphatase; PF01300 Sua5_yciO_yrdC; PF07503 zf-HYPF [H]
EC number: NA
Molecular weight: Translated: 84191; Mature: 84060
Theoretical pI: Translated: 8.33; Mature: 8.33
Prosite motif: PS00150 ACYLPHOSPHATASE_1 ; PS51160 ACYLPHOSPHATASE_3 ; PS00213 LIPOCALIN ; PS51163 YRDC
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
2.7 %Cys (Translated Protein) 2.0 %Met (Translated Protein) 4.7 %Cys+Met (Translated Protein) 2.7 %Cys (Mature Protein) 1.8 %Met (Mature Protein) 4.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MAQRSNAEKKGIVRYRIIVKGIVQGVGFRPFIYNLARLYRLKGTVANTSQGVLIEAEGQE CCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCH ETVHAFLASLKEKHPPLSRITALEWDVLEPCGYSSFAIIPSGEGLGKEALIPPDVALCAD HHHHHHHHHHHHCCCCHHHHEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHH CAREIMDPRDRHYGYPFTNCTNCGPRFTIVRGVPYDRAKTSMARFPMCPECAREYHDPGN HHHHHCCCCHHCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCHHHHHHHCCCCC RRFHAQPAACPACGPQVELVDRQGRKVEGNWLELSWRFLQDGKILAVKGLGGFHLVCDAK CEEECCCCCCCCCCCCEEEECCCCCEECCCEEEEEEEEECCCCEEEEECCCCEEEEEECC NRAALKTLRRRKGREAKPLAVMCLLETARKYCYVGPEEEKLLSSPQAPIVILTKRADCNL CHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHEECCCCHHHHHCCCCCCEEEEEECCCCCC PDELAPGMKTLGIMLPYTPLHLMLLNGPLEILVMTSGNRNGLPLAKDNGRALEELGGIAD CHHHCCCHHHHHEECCCCCEEEEEECCCEEEEEEECCCCCCCCCCCCCCHHHHHHCCHHH YFLWHNREIVNRCDDSVVAVIGDTAQILRRSRGYVPSPVKVAVKSSSPVLGAGGDMKNTF HHHHCCHHHHHHCCCCEEEEECCHHHHHHHHCCCCCCCEEEEEECCCCEEECCCCCCCEE CLLKGNQAFVSQHIGDLGSREGEAHFFASLENLKNLIGSEPEVVGYDMHPGYRSSRLAAG EEEECCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEECC IPAKAHFAVQHHHAHMVSCLADNGVDEDAIGVILDGTGYGTDGRLWGFEILTGDCADFTR CCCHHEEEEHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCEEEEEEEECCHHHHCC EYHLAYVPLPGGEQAVRYPWRTAVAYLMKYLSAQGESLADRLFQSRGQELEVIKRLVATG CEEEEEEECCCCCCHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHCC FNSPLSSSCGRLFDAVSALLGLCYHNSYEGQAAIELGEMVLDPAEGKRLIPYPFFIEGKV CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCEECCCCEEEECEE IHPGGVIAGVAADLERGVAREIIATRFHNTVLAMVREAVRRVAERTHIKTVALSGGAWQN ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCH RYLFSLAKEILPGDGYRLLVHRQVPANDGGLSLGQAVIACRRWQQCV HHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCC >Mature Secondary Structure AQRSNAEKKGIVRYRIIVKGIVQGVGFRPFIYNLARLYRLKGTVANTSQGVLIEAEGQE CCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCH ETVHAFLASLKEKHPPLSRITALEWDVLEPCGYSSFAIIPSGEGLGKEALIPPDVALCAD HHHHHHHHHHHHCCCCHHHHEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHH CAREIMDPRDRHYGYPFTNCTNCGPRFTIVRGVPYDRAKTSMARFPMCPECAREYHDPGN HHHHHCCCCHHCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCHHHHHHHCCCCC RRFHAQPAACPACGPQVELVDRQGRKVEGNWLELSWRFLQDGKILAVKGLGGFHLVCDAK CEEECCCCCCCCCCCCEEEECCCCCEECCCEEEEEEEEECCCCEEEEECCCCEEEEEECC NRAALKTLRRRKGREAKPLAVMCLLETARKYCYVGPEEEKLLSSPQAPIVILTKRADCNL CHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHEECCCCHHHHHCCCCCCEEEEEECCCCCC PDELAPGMKTLGIMLPYTPLHLMLLNGPLEILVMTSGNRNGLPLAKDNGRALEELGGIAD CHHHCCCHHHHHEECCCCCEEEEEECCCEEEEEEECCCCCCCCCCCCCCHHHHHHCCHHH YFLWHNREIVNRCDDSVVAVIGDTAQILRRSRGYVPSPVKVAVKSSSPVLGAGGDMKNTF HHHHCCHHHHHHCCCCEEEEECCHHHHHHHHCCCCCCCEEEEEECCCCEEECCCCCCCEE CLLKGNQAFVSQHIGDLGSREGEAHFFASLENLKNLIGSEPEVVGYDMHPGYRSSRLAAG EEEECCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEECC IPAKAHFAVQHHHAHMVSCLADNGVDEDAIGVILDGTGYGTDGRLWGFEILTGDCADFTR CCCHHEEEEHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCEEEEEEEECCHHHHCC EYHLAYVPLPGGEQAVRYPWRTAVAYLMKYLSAQGESLADRLFQSRGQELEVIKRLVATG CEEEEEEECCCCCCHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHCC FNSPLSSSCGRLFDAVSALLGLCYHNSYEGQAAIELGEMVLDPAEGKRLIPYPFFIEGKV CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCEECCCCEEEECEE IHPGGVIAGVAADLERGVAREIIATRFHNTVLAMVREAVRRVAERTHIKTVALSGGAWQN ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCH RYLFSLAKEILPGDGYRLLVHRQVPANDGGLSLGQAVIACRRWQQCV HHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 8497190; 9492269 [H]