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The map label for this gene is mtbC [H]

Identifier: 83590942

GI number: 83590942

Start: 2211818

End: 2212447

Strand: Reverse

Name: mtbC [H]

Synonym: Moth_2116

Alternate gene names: 83590942

Gene position: 2212447-2211818 (Counterclockwise)

Preceding gene: 83590943

Following gene: 83590941

Centisome position: 84.16

GC content: 54.29

Gene sequence:

>630_bases
ATGGCTGTCCTGGAGGAAATGAAAGAAGCCTTGATGGCTGGTAACGCCAACAAAGTGCGGGAACTGGCCCAAAAAGCCCT
AGATGAAGGCATTGAGCCCACGACGATCATTAACGACGGCCTGATTGCAGCCATGAACATTATTGGTGTCAAATTTAAGA
ATAATGAAGTCTATGTTCCGGAGGTCCTGGTAGCGGCCCGGGCCATGCACGCTGGCATGGATGTGGTCAAGCCCCTCCTG
GCCGGCGGCGACATTAAGGAAAAAGGCACCTTCGTTATCGGTACGGTCAAGGGTGACCTTCACGATATCGGCAAGAACCT
GGTCATCATGATGATGGAAGGCGCGGGCTACAAGGTTATCGACCTAGGTATTGACGTGCCGGTAGAGAAATTCGTCCAGG
CGGTGGAAGAGCACAAACCCCAGCTGGTAGGCATTTCCGCCCTGCTGACATCCACCATGATGCAGATGAAGAAGACTGTC
GAAGGTCTGGCTGCTTACAGGGATAAGATCAAGATTATGGTCGGCGGCGCCCCGGTAACCCAGAAGTTTGCCGATGAAAT
CGGAGCCGACGGTTACGCTCCCGATGCTGCGTCGGCCGTTGACCTGGCCGCACAACTCCTCGCTTCGTAA

Upstream 100 bases:

>100_bases
AAGAAAATAAACCTTTGGCAAGAATTTAACCTTGCCGTTCAATTTATCTTAAAATAAACTTAAAAATGAACCAACTTTAA
TATTTAAGGAGGAATGACAG

Downstream 100 bases:

>100_bases
AATATAAGGGAGGCCAAGTGATGCTCGTTGTCGGAGAGTTAATTAACTCCAGCCGCAAAGCCATTGCCCAGGCTATTGCT
AACCGGGATAAGGATTATAT

Product: corrinoid methyltransferase

Products: L-Methionine; 5,6,7,8-Tetrahydrofolate [C]

Alternate protein names: NA

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MAVLEEMKEALMAGNANKVRELAQKALDEGIEPTTIINDGLIAAMNIIGVKFKNNEVYVPEVLVAARAMHAGMDVVKPLL
AGGDIKEKGTFVIGTVKGDLHDIGKNLVIMMMEGAGYKVIDLGIDVPVEKFVQAVEEHKPQLVGISALLTSTMMQMKKTV
EGLAAYRDKIKIMVGGAPVTQKFADEIGADGYAPDAASAVDLAAQLLAS

Sequences:

>Translated_209_residues
MAVLEEMKEALMAGNANKVRELAQKALDEGIEPTTIINDGLIAAMNIIGVKFKNNEVYVPEVLVAARAMHAGMDVVKPLL
AGGDIKEKGTFVIGTVKGDLHDIGKNLVIMMMEGAGYKVIDLGIDVPVEKFVQAVEEHKPQLVGISALLTSTMMQMKKTV
EGLAAYRDKIKIMVGGAPVTQKFADEIGADGYAPDAASAVDLAAQLLAS
>Mature_208_residues
AVLEEMKEALMAGNANKVRELAQKALDEGIEPTTIINDGLIAAMNIIGVKFKNNEVYVPEVLVAARAMHAGMDVVKPLLA
GGDIKEKGTFVIGTVKGDLHDIGKNLVIMMMEGAGYKVIDLGIDVPVEKFVQAVEEHKPQLVGISALLTSTMMQMKKTVE
GLAAYRDKIKIMVGGAPVTQKFADEIGADGYAPDAASAVDLAAQLLAS

Specific function: Acts as a methyl group carrier between mtbB and mtbA [H]

COG id: COG5012

COG function: function code R; Predicted cobalamin binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=198, Percent_Identity=32.3232323232323, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI1790450, Length=214, Percent_Identity=27.5700934579439, Blast_Score=99, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17535405, Length=197, Percent_Identity=29.4416243654822, Blast_Score=87, Evalue=6e-18,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR003759
- InterPro:   IPR012741 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2 [H]

EC number: 2.1.1.13 [C]

Molecular weight: Translated: 22149; Mature: 22018

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
6.2 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.8 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVLEEMKEALMAGNANKVRELAQKALDEGIEPTTIINDGLIAAMNIIGVKFKNNEVYVP
CHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHEEEECCCEEEHH
EVLVAARAMHAGMDVVKPLLAGGDIKEKGTFVIGTVKGDLHDIGKNLVIMMMEGAGYKVI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHCCCEEEEEECCCCEEEE
DLGIDVPVEKFVQAVEEHKPQLVGISALLTSTMMQMKKTVEGLAAYRDKIKIMVGGAPVT
EECCCCCHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHH
QKFADEIGADGYAPDAASAVDLAAQLLAS
HHHHHHHCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
AVLEEMKEALMAGNANKVRELAQKALDEGIEPTTIINDGLIAAMNIIGVKFKNNEVYVP
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHEEEECCCEEEHH
EVLVAARAMHAGMDVVKPLLAGGDIKEKGTFVIGTVKGDLHDIGKNLVIMMMEGAGYKVI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHCCCEEEEEECCCCEEEE
DLGIDVPVEKFVQAVEEHKPQLVGISALLTSTMMQMKKTVEGLAAYRDKIKIMVGGAPVT
EECCCCCHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHH
QKFADEIGADGYAPDAASAVDLAAQLLAS
HHHHHHHCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: ATP; Cobinamide; S-adenosyl-L-methionine [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 500-780 [C]

Specific activity: 3.8

Km value (mM): 0.0016 {S-adenosyl-L-methionine}} 0.00065 {S-adenosyl-L-methionine}} 0.06 {methyltetrahydrofolate}} 0.03 {methyltetrahydrofolate}} 0.016 {homocysteine}} 0.035 {S-methyltetrahydrofolate}} [C]

Substrates: 5-Methyltetrahydrofolate; L-Homocysteine [C]

Specific reaction: 5-Methyltetrahydrofolate + L-Homocysteine --> L-Methionine + 5,6,7,8-Tetrahydrofolate [C]

General reaction: Methyl group transfer [C]

Inhibitor: 5, 6-Dimethyl benzimidazole; alpha-Ribazole 3'-phosphate; Be2+; Cobinamide; Propyl iodide; Sr2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10762254; 10852929 [H]