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The map label for this gene is metH [H]

Identifier: 83590941

GI number: 83590941

Start: 2211000

End: 2211797

Strand: Reverse

Name: metH [H]

Synonym: Moth_2115

Alternate gene names: 83590941

Gene position: 2211797-2211000 (Counterclockwise)

Preceding gene: 83590942

Following gene: 83590940

Centisome position: 84.14

GC content: 55.64

Gene sequence:

>798_bases
ATGCTCGTTGTCGGAGAGTTAATTAACTCCAGCCGCAAAGCCATTGCCCAGGCTATTGCTAACCGGGATAAGGATTATAT
CCAGGAACTGGCCAGGAAGCAGGCCGAGGCCGGAGCCGATATTATCGATGTTAACTGCGGGACAAGCATCGGCGAAGAAA
ATAAGGTAATGACCTGGCTCGTGAATATCGTCCAGGAAGTCGTTGATGTTCCCCTGTGTATTGATAGCCCCAGCGCCGAG
GCCCTGGCTGCCGGCCTGGCAGCCCATAAGGGCCAGGCAATGGTAAACTCCATCACCGCCGAGAAGCAGCGCTGGGAGGA
AGTTTTGCCTTTAGTGCAGAAATATAAGGCTAAGGTTATCGCCCTTTGCATGGATGACGGCGGCATGCCGGATTCGGTAG
AGGACCGCCTTCGCGTTGCTGGTAAGCTGGTACCCGGGCTGGTAGAGGCCGGCATACCGGAAGACGATATCTACCTTGAT
CCCCTGATTAAACCCCTGGGAGTTAACAGCCAGTACGGCGTGGAGGCTCTGGAATCGGTTGCCGCCCTGCGGCAAAAATA
CCCCGGGGTTCACACCATCTGCGGCCTTTCCAACGTTTCCTATGGCCTGCCGGAAAGGCGACTGCTGAACCGGGCCTTTA
TGGTTATGTGCCTCACTAAAGGTATGGATTCCTTTATCCTTGATCCCCTGGATATGCCATTAATGGGCCTGTTGCGGGCC
GCCACCGCCCTGTACGGCCAGGATGAATACTGCCTGGAGTATATATCTGCCGCCCGGGCCGGGAAGATTAAGGCCTGA

Upstream 100 bases:

>100_bases
CCGATGAAATCGGAGCCGACGGTTACGCTCCCGATGCTGCGTCGGCCGTTGACCTGGCCGCACAACTCCTCGCTTCGTAA
AATATAAGGGAGGCCAAGTG

Downstream 100 bases:

>100_bases
TCTGGCAAGCAACTTGCGGCCGCAGGAGGGAGGTTTCCCTACTGCGGTTGTTTTATTAGGAGGAGTAATATGGCATCCTT
TTCCCTGGAAAATCTAATCC

Product: methyltetrahydrofolate:corrinoid/iron-sulfur protein methyltransferase

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MLVVGELINSSRKAIAQAIANRDKDYIQELARKQAEAGADIIDVNCGTSIGEENKVMTWLVNIVQEVVDVPLCIDSPSAE
ALAAGLAAHKGQAMVNSITAEKQRWEEVLPLVQKYKAKVIALCMDDGGMPDSVEDRLRVAGKLVPGLVEAGIPEDDIYLD
PLIKPLGVNSQYGVEALESVAALRQKYPGVHTICGLSNVSYGLPERRLLNRAFMVMCLTKGMDSFILDPLDMPLMGLLRA
ATALYGQDEYCLEYISAARAGKIKA

Sequences:

>Translated_265_residues
MLVVGELINSSRKAIAQAIANRDKDYIQELARKQAEAGADIIDVNCGTSIGEENKVMTWLVNIVQEVVDVPLCIDSPSAE
ALAAGLAAHKGQAMVNSITAEKQRWEEVLPLVQKYKAKVIALCMDDGGMPDSVEDRLRVAGKLVPGLVEAGIPEDDIYLD
PLIKPLGVNSQYGVEALESVAALRQKYPGVHTICGLSNVSYGLPERRLLNRAFMVMCLTKGMDSFILDPLDMPLMGLLRA
ATALYGQDEYCLEYISAARAGKIKA
>Mature_265_residues
MLVVGELINSSRKAIAQAIANRDKDYIQELARKQAEAGADIIDVNCGTSIGEENKVMTWLVNIVQEVVDVPLCIDSPSAE
ALAAGLAAHKGQAMVNSITAEKQRWEEVLPLVQKYKAKVIALCMDDGGMPDSVEDRLRVAGKLVPGLVEAGIPEDDIYLD
PLIKPLGVNSQYGVEALESVAALRQKYPGVHTICGLSNVSYGLPERRLLNRAFMVMCLTKGMDSFILDPLDMPLMGLLRA
ATALYGQDEYCLEYISAARAGKIKA

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG1410

COG function: function code E; Methionine synthase I, cobalamin-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=219, Percent_Identity=26.4840182648402, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI1790450, Length=241, Percent_Identity=25.7261410788382, Blast_Score=80, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17535405, Length=246, Percent_Identity=25.2032520325203, Blast_Score=95, Evalue=3e-20,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 28705; Mature: 28705

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS50972 PTERIN_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVVGELINSSRKAIAQAIANRDKDYIQELARKQAEAGADIIDVNCGTSIGEENKVMTWL
CEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHH
VNIVQEVVDVPLCIDSPSAEALAAGLAAHKGQAMVNSITAEKQRWEEVLPLVQKYKAKVI
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
ALCMDDGGMPDSVEDRLRVAGKLVPGLVEAGIPEDDIYLDPLIKPLGVNSQYGVEALESV
EEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHH
AALRQKYPGVHTICGLSNVSYGLPERRLLNRAFMVMCLTKGMDSFILDPLDMPLMGLLRA
HHHHHHCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHH
ATALYGQDEYCLEYISAARAGKIKA
HHHHHCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLVVGELINSSRKAIAQAIANRDKDYIQELARKQAEAGADIIDVNCGTSIGEENKVMTWL
CEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHH
VNIVQEVVDVPLCIDSPSAEALAAGLAAHKGQAMVNSITAEKQRWEEVLPLVQKYKAKVI
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
ALCMDDGGMPDSVEDRLRVAGKLVPGLVEAGIPEDDIYLDPLIKPLGVNSQYGVEALESV
EEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHH
AALRQKYPGVHTICGLSNVSYGLPERRLLNRAFMVMCLTKGMDSFILDPLDMPLMGLLRA
HHHHHHCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHH
ATALYGQDEYCLEYISAARAGKIKA
HHHHHCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]