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The map label for this gene is nadE [H]

Identifier: 83590913

GI number: 83590913

Start: 2178280

End: 2179017

Strand: Reverse

Name: nadE [H]

Synonym: Moth_2086

Alternate gene names: 83590913

Gene position: 2179017-2178280 (Counterclockwise)

Preceding gene: 83590915

Following gene: 83590909

Centisome position: 82.89

GC content: 63.82

Gene sequence:

>738_bases
TTGCGTCTAGGTGCGGCCCAGATGTTTATCGCCGACAGCATGGCGGTCAATGAGGCGACAATCTTGCGCCTGGCCGGGGA
AGCAGCCGGGCGGGGCGTGGAACTGCTGGTTTTCCCGGAAATGGGCCTGACGGGGTATAACCCGGCTGCCCTGGGCCGGC
CGGGGTTTAAGGAAGAGCTGGAGGGCGCCCTGGCCAGGATTGCCAGGAGAGCAGCAGACCTGGGGGTGGGTTTGATTGTC
GGCCGGGCCGAGTTTGCCGGCGAACGATTGTTCAACTCCGCCTCGGTGTTCCTGCCCGACGGCAGCGTCCATACCTACCG
CAAGATCTACCTGACGGACGCCGAGGCCCGCTATTTTACCCCGGGCACCGGGCATCTGGTCTTTAACTATAAGGGGAGCA
AATTCGGGGTCATCATCTGCCGCGATCAGAATTACCCCGAACTGGCCCGGCAGATAGCGGCGGAAGGAGCACGGGCCCTC
TTCATCCTTTCCGCCCACTACTACCAACCGGGCGAGGCCCGCTGGAAGCTGCCCAAAAACCGTGCCCTGCCCATCGCCAG
GGCGGTGGAGAACCACTGCTACGTCCTCCTGGCCAACGCCGTGGGCAGCCACATCGGTATGGTGAGCCTGGGAAACAGCC
TGATCGCCGACCCCGAGGGCGGCCTGGTAGTTATGGCTGATGAAGCCTCGGAGACGCTGCTGACCTGCGACCTGCCGGGG
GACAGCCAGTGTAGCTGA

Upstream 100 bases:

>100_bases
CAAAGATAATCACAAGCCGGGTGACTTACGCTGAGCACAACCTGCAGATGTGGAGTAGTATCCGCAAGATGGGAGCGAAA
ATACCACGGGGGCGATGATT

Downstream 100 bases:

>100_bases
AAGGGGTAAGACCGGCCGGTGCAGCAGCAAAAACGCCGGTTCCATGTGGCCAGCCCCTACCGCGACACCCTGCGGAAGGC
CAGATAAGAATAAACCGTGG

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolysing] [H]

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MRLGAAQMFIADSMAVNEATILRLAGEAAGRGVELLVFPEMGLTGYNPAALGRPGFKEELEGALARIARRAADLGVGLIV
GRAEFAGERLFNSASVFLPDGSVHTYRKIYLTDAEARYFTPGTGHLVFNYKGSKFGVIICRDQNYPELARQIAAEGARAL
FILSAHYYQPGEARWKLPKNRALPIARAVENHCYVLLANAVGSHIGMVSLGNSLIADPEGGLVVMADEASETLLTCDLPG
DSQCS

Sequences:

>Translated_245_residues
MRLGAAQMFIADSMAVNEATILRLAGEAAGRGVELLVFPEMGLTGYNPAALGRPGFKEELEGALARIARRAADLGVGLIV
GRAEFAGERLFNSASVFLPDGSVHTYRKIYLTDAEARYFTPGTGHLVFNYKGSKFGVIICRDQNYPELARQIAAEGARAL
FILSAHYYQPGEARWKLPKNRALPIARAVENHCYVLLANAVGSHIGMVSLGNSLIADPEGGLVVMADEASETLLTCDLPG
DSQCS
>Mature_245_residues
MRLGAAQMFIADSMAVNEATILRLAGEAAGRGVELLVFPEMGLTGYNPAALGRPGFKEELEGALARIARRAADLGVGLIV
GRAEFAGERLFNSASVFLPDGSVHTYRKIYLTDAEARYFTPGTGHLVFNYKGSKFGVIICRDQNYPELARQIAAEGARAL
FILSAHYYQPGEARWKLPKNRALPIARAVENHCYVLLANAVGSHIGMVSLGNSLIADPEGGLVVMADEASETLLTCDLPG
DSQCS

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=240, Percent_Identity=28.75, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI297632350, Length=236, Percent_Identity=29.2372881355932, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI297632348, Length=236, Percent_Identity=29.2372881355932, Blast_Score=65, Evalue=6e-11,
Organism=Homo sapiens, GI5031947, Length=236, Percent_Identity=29.2372881355932, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1786412, Length=210, Percent_Identity=24.7619047619048, Blast_Score=64, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323383, Length=181, Percent_Identity=29.8342541436464, Blast_Score=76, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6322335, Length=272, Percent_Identity=25, Blast_Score=73, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR000132
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 26259; Mature: 26259

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLGAAQMFIADSMAVNEATILRLAGEAAGRGVELLVFPEMGLTGYNPAALGRPGFKEEL
CCCCCHHEEEECCCCCCCEEEEEEECCCCCCCEEEEEECCCCCCCCCCHHCCCCCCHHHH
EGALARIARRAADLGVGLIVGRAEFAGERLFNSASVFLPDGSVHTYRKIYLTDAEARYFT
HHHHHHHHHHHHHCCEEEEEECHHHHHHHHHCCCEEEECCCCCEEEEEEEEEECCCEEEC
PGTGHLVFNYKGSKFGVIICRDQNYPELARQIAAEGARALFILSAHYYQPGEARWKLPKN
CCCCEEEEEECCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEEEEECCCCCCCEEECCCC
RALPIARAVENHCYVLLANAVGSHIGMVSLGNSLIADPEGGLVVMADEASETLLTCDLPG
CCCHHHHHHCCCEEEEEECHHCCCCCEEECCCCEEECCCCCEEEEECCCCCEEEEEECCC
DSQCS
CCCCC
>Mature Secondary Structure
MRLGAAQMFIADSMAVNEATILRLAGEAAGRGVELLVFPEMGLTGYNPAALGRPGFKEEL
CCCCCHHEEEECCCCCCCEEEEEEECCCCCCCEEEEEECCCCCCCCCCHHCCCCCCHHHH
EGALARIARRAADLGVGLIVGRAEFAGERLFNSASVFLPDGSVHTYRKIYLTDAEARYFT
HHHHHHHHHHHHHCCEEEEEECHHHHHHHHHCCCEEEECCCCCEEEEEEEEEECCCEEEC
PGTGHLVFNYKGSKFGVIICRDQNYPELARQIAAEGARALFILSAHYYQPGEARWKLPKN
CCCCEEEEEECCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEEEEECCCCCCCEEECCCC
RALPIARAVENHCYVLLANAVGSHIGMVSLGNSLIADPEGGLVVMADEASETLLTCDLPG
CCCHHHHHHCCCEEEEEECHHCCCCCEEECCCCEEECCCCCEEEEECCCCCEEEEEECCC
DSQCS
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]