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The map label for this gene is aprN [H]

Identifier: 83590857

GI number: 83590857

Start: 2116223

End: 2117422

Strand: Reverse

Name: aprN [H]

Synonym: Moth_2027

Alternate gene names: 83590857

Gene position: 2117422-2116223 (Counterclockwise)

Preceding gene: 83590858

Following gene: 83590856

Centisome position: 80.55

GC content: 59.25

Gene sequence:

>1200_bases
GTGTGGCCGGTTTTACTGGCTTATTATACCGGCGTAGCGACGGCGGCAGGTGCCATGATCGCCTGGTCCGGCAACAAAAA
AGTAGGTAAGCGGAAGATTATCCTTTTTCACCAGGAGAAACCCCTACCCAGTTGCCGCGCCCTGTTGCAAAAGAGGGGCG
GCCAGGTATTAAAGGAATTACCCCTGGTGCATGCCCTTGTGGCCCGACTACCTGCAAAGGGAAAAGTAATTGAAGAACTG
GGTTTACACCCTGACATTCGCCTGATTGAAGACGATTTTGAAGTTCACACGGTGGAGTTGCCGGCCGCCCGGATACGGCA
GGAGAAACAGATAGTTCCCTGGGGCGTTGAGCGCATCGGCGCTCCGAGGGCCTGGCAGGTGGCTGCCGGGGAAAAGGTGA
AGGTGGCGGTCCTAGACACAGGCCTTGATGCCGGGCATCCCGACCTGGCGGCCAACGTCCGCGGTACCCAGAATATAAAA
TTTCCCGGCTGGCGGGCCGGAGACGGGAACGGCCACGGTACCCATGTAGCAGGGATTATTGCCGCCCTGAACAACAGCTT
CGGGGTGGTAGGGGTTGCGCCCCGGGCCGAGATTTATGGAGTAAAGATTTTTAACCGTCAAGGTGACGGTTATATATCCG
ATATCGTAGCCGGCCTGGACTGGGCGCTAAAAAATAAGATGCAGGTAGTAAACATGAGCTTTGGCACCAGCCAACCCAGC
CAGGCCCTGGAGGAGGCCGTCCGCAAATGTGTCCAGGCGGGAATGGTGCTGGTTGCGGCGGCCGGAAACGAAGGCAGGGA
CGATAGTGTTCTATATCCGGCCCGCTATCCGGGGGTCATCGCCGTTTCGGCCGTCGATAAGAAGGATAACCTGGCCAGCT
TTAGCAGCCGGGGAACGGAGGTAACGGTCACTGCTCCCGGAGTCGATATCCTATCTACTTACCCGGGGGGCAAATACCGG
ACTATGAGCGGAACTTCCATGGCCTGCCCCCACGCCGCCGGGGTGGCGGCCCTGATTCTGGCCCAGGATAGGCGCCTATC
CGGCCGGCAGGTGGCCAGGATAATCTGCCGCACGGCCATTAAGTTACCCGATCTGTCACCCCGGGAACAGGGGGACGGTT
TGGTCAACGCCACCGCCCTGGCGGCCGTAGCCCGCTTTATTGGGGAAACGGGAACGGCAGGCGAGGAGTCGGTCGGCTAA

Upstream 100 bases:

>100_bases
GGGAGGGAGAAAAATTCTTGCGGTTGACCAGGTACCTCCAGGAGAACCCCTGCATAGGATGCAGCATAATCATGATATCT
AATTCCTGGAGGGATTGTCC

Downstream 100 bases:

>100_bases
AAGAGTTACTAATTCTAGGTTTTGGAGTAAACGACCGTTGGCGCGGTCGTCTCTTTTTTTGTACCGTAAAATTTTTTTAA
AAAAATATTAAGAACCAGCA

Product: aerolysin

Products: NA

Alternate protein names: Nattokinase [H]

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MWPVLLAYYTGVATAAGAMIAWSGNKKVGKRKIILFHQEKPLPSCRALLQKRGGQVLKELPLVHALVARLPAKGKVIEEL
GLHPDIRLIEDDFEVHTVELPAARIRQEKQIVPWGVERIGAPRAWQVAAGEKVKVAVLDTGLDAGHPDLAANVRGTQNIK
FPGWRAGDGNGHGTHVAGIIAALNNSFGVVGVAPRAEIYGVKIFNRQGDGYISDIVAGLDWALKNKMQVVNMSFGTSQPS
QALEEAVRKCVQAGMVLVAAAGNEGRDDSVLYPARYPGVIAVSAVDKKDNLASFSSRGTEVTVTAPGVDILSTYPGGKYR
TMSGTSMACPHAAGVAALILAQDRRLSGRQVARIICRTAIKLPDLSPREQGDGLVNATALAAVARFIGETGTAGEESVG

Sequences:

>Translated_399_residues
MWPVLLAYYTGVATAAGAMIAWSGNKKVGKRKIILFHQEKPLPSCRALLQKRGGQVLKELPLVHALVARLPAKGKVIEEL
GLHPDIRLIEDDFEVHTVELPAARIRQEKQIVPWGVERIGAPRAWQVAAGEKVKVAVLDTGLDAGHPDLAANVRGTQNIK
FPGWRAGDGNGHGTHVAGIIAALNNSFGVVGVAPRAEIYGVKIFNRQGDGYISDIVAGLDWALKNKMQVVNMSFGTSQPS
QALEEAVRKCVQAGMVLVAAAGNEGRDDSVLYPARYPGVIAVSAVDKKDNLASFSSRGTEVTVTAPGVDILSTYPGGKYR
TMSGTSMACPHAAGVAALILAQDRRLSGRQVARIICRTAIKLPDLSPREQGDGLVNATALAAVARFIGETGTAGEESVG
>Mature_399_residues
MWPVLLAYYTGVATAAGAMIAWSGNKKVGKRKIILFHQEKPLPSCRALLQKRGGQVLKELPLVHALVARLPAKGKVIEEL
GLHPDIRLIEDDFEVHTVELPAARIRQEKQIVPWGVERIGAPRAWQVAAGEKVKVAVLDTGLDAGHPDLAANVRGTQNIK
FPGWRAGDGNGHGTHVAGIIAALNNSFGVVGVAPRAEIYGVKIFNRQGDGYISDIVAGLDWALKNKMQVVNMSFGTSQPS
QALEEAVRKCVQAGMVLVAAAGNEGRDDSVLYPARYPGVIAVSAVDKKDNLASFSSRGTEVTVTAPGVDILSTYPGGKYR
TMSGTSMACPHAAGVAALILAQDRRLSGRQVARIICRTAIKLPDLSPREQGDGLVNATALAAVARFIGETGTAGEESVG

Specific function: Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides. Subtilisin NAT also has fibrinolytic activity [H]

COG id: COG1404

COG function: function code O; Subtilisin-like serine proteases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S8 family [H]

Homologues:

Organism=Homo sapiens, GI4506775, Length=275, Percent_Identity=32.7272727272727, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI31317307, Length=323, Percent_Identity=31.5789473684211, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI20336190, Length=311, Percent_Identity=27.9742765273312, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI20336188, Length=311, Percent_Identity=27.9742765273312, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI27894285, Length=305, Percent_Identity=27.8688524590164, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI20336180, Length=311, Percent_Identity=27.9742765273312, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI20336182, Length=311, Percent_Identity=27.9742765273312, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI20336184, Length=311, Percent_Identity=27.9742765273312, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI4505577, Length=311, Percent_Identity=27.9742765273312, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI20336186, Length=365, Percent_Identity=24.9315068493151, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI299523015, Length=274, Percent_Identity=28.1021897810219, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI4505579, Length=269, Percent_Identity=28.996282527881, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI20336246, Length=273, Percent_Identity=28.2051282051282, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71983555, Length=269, Percent_Identity=28.996282527881, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI25141268, Length=269, Percent_Identity=28.996282527881, Blast_Score=79, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6324576, Length=292, Percent_Identity=34.2465753424658, Blast_Score=139, Evalue=7e-34,
Organism=Saccharomyces cerevisiae, GI6320775, Length=291, Percent_Identity=33.3333333333333, Blast_Score=124, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6319893, Length=258, Percent_Identity=33.7209302325581, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6324091, Length=261, Percent_Identity=30.6513409961686, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI45550681, Length=276, Percent_Identity=30.7971014492754, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI45553515, Length=307, Percent_Identity=27.6872964169381, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24650058, Length=307, Percent_Identity=27.6872964169381, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24650060, Length=307, Percent_Identity=27.6872964169381, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI45553513, Length=307, Percent_Identity=27.6872964169381, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24650056, Length=307, Percent_Identity=27.6872964169381, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI17864552, Length=307, Percent_Identity=27.6872964169381, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17136192, Length=376, Percent_Identity=27.1276595744681, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24653270, Length=264, Percent_Identity=31.8181818181818, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI17137360, Length=264, Percent_Identity=31.8181818181818, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000209
- InterPro:   IPR022398
- InterPro:   IPR015500
- InterPro:   IPR009020
- InterPro:   IPR010259 [H]

Pfam domain/function: PF05922 Inhibitor_I9; PF00082 Peptidase_S8 [H]

EC number: =3.4.21.62 [H]

Molecular weight: Translated: 42267; Mature: 42267

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: PS00136 SUBTILASE_ASP ; PS00137 SUBTILASE_HIS ; PS00138 SUBTILASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWPVLLAYYTGVATAAGAMIAWSGNKKVGKRKIILFHQEKPLPSCRALLQKRGGQVLKEL
CCCCHHHHHHHHHHHCCEEEEECCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCHHHHHH
PLVHALVARLPAKGKVIEELGLHPDIRLIEDDFEVHTVELPAARIRQEKQIVPWGVERIG
HHHHHHHHHCCCCCCHHHHHCCCCCEEEEECCCEEEEEECCHHHHHHHCCCCCCCHHHCC
APRAWQVAAGEKVKVAVLDTGLDAGHPDLAANVRGTQNIKFPGWRAGDGNGHGTHVAGII
CCCEEEECCCCEEEEEEEECCCCCCCCCCEECCCCCCCCCCCCEECCCCCCCCHHHHHHH
AALNNSFGVVGVAPRAEIYGVKIFNRQGDGYISDIVAGLDWALKNKMQVVNMSFGTSQPS
HHHCCCCCEEEECCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCHH
QALEEAVRKCVQAGMVLVAAAGNEGRDDSVLYPARYPGVIAVSAVDKKDNLASFSSRGTE
HHHHHHHHHHHHCCEEEEEECCCCCCCCCEEECCCCCCEEEEEEECCCCCHHHHHCCCCE
VTVTAPGVDILSTYPGGKYRTMSGTSMACPHAAGVAALILAQDRRLSGRQVARIICRTAI
EEEEECCCCEEECCCCCCEEECCCCCCCCCCHHHHHEEEEECCCCCCHHHHHHHHHHHHH
KLPDLSPREQGDGLVNATALAAVARFIGETGTAGEESVG
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MWPVLLAYYTGVATAAGAMIAWSGNKKVGKRKIILFHQEKPLPSCRALLQKRGGQVLKEL
CCCCHHHHHHHHHHHCCEEEEECCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCHHHHHH
PLVHALVARLPAKGKVIEELGLHPDIRLIEDDFEVHTVELPAARIRQEKQIVPWGVERIG
HHHHHHHHHCCCCCCHHHHHCCCCCEEEEECCCEEEEEECCHHHHHHHCCCCCCCHHHCC
APRAWQVAAGEKVKVAVLDTGLDAGHPDLAANVRGTQNIKFPGWRAGDGNGHGTHVAGII
CCCEEEECCCCEEEEEEEECCCCCCCCCCEECCCCCCCCCCCCEECCCCCCCCHHHHHHH
AALNNSFGVVGVAPRAEIYGVKIFNRQGDGYISDIVAGLDWALKNKMQVVNMSFGTSQPS
HHHCCCCCEEEECCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCHH
QALEEAVRKCVQAGMVLVAAAGNEGRDDSVLYPARYPGVIAVSAVDKKDNLASFSSRGTE
HHHHHHHHHHHHCCEEEEEECCCCCCCCCEEECCCCCCEEEEEEECCCCCHHHHHCCCCE
VTVTAPGVDILSTYPGGKYRTMSGTSMACPHAAGVAALILAQDRRLSGRQVARIICRTAI
EEEEECCCCEEECCCCCCEEECCCCCCCCCCHHHHHEEEEECCCCCCHHHHHHHHHHHHH
KLPDLSPREQGDGLVNATALAAVARFIGETGTAGEESVG
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1369081 [H]