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The map label for this gene is mhpE [C]

Identifier: 83590610

GI number: 83590610

Start: 1830419

End: 1831435

Strand: Reverse

Name: mhpE [C]

Synonym: Moth_1775

Alternate gene names: 83590610

Gene position: 1831435-1830419 (Counterclockwise)

Preceding gene: 83590611

Following gene: 83590609

Centisome position: 69.67

GC content: 58.8

Gene sequence:

>1017_bases
ATGAGCGCTAGGTCAATCCACATTGTCGATACAACCCTGCGTGACGGTAGTCACGCCGTCAGCCACCAGTTTACGGCCGA
GCAAATAGCCGCCATTGCCGGCGGCCTGGACGCGGCCGGAGTGGAGTATATCGAGGTTTCCCACGGTGACGGCCTGGCCG
GTTCCTCTTACAACTACGGCTGGGCAGCCCTGGGGGATGAGGAAATGCTAAAAGCCGCTAGCGCGGCGATAAAAAAAGGC
AAACTAACTGTCCTCTTACTCCCCGGCATTGGCACCGTCGAGGACCTGAAGATGGCGGCCGACTGCGGCGCCAAAGTGGT
CCGGGTGGCCACCCACGTCACCGAGGCCGATATCGGCGAGCAGCATATCGGCATGGCCAAGAAGCTGGGTATGATGGCTG
TTGGTTTCCTTATGATGTGCCACATGGCGCCGCCGGAAAAGGTAGTGGAACAGGCCAAACTCTTTGAGTCCTACGGCGCC
GACTATATCAACATCGCCGACTCCGCGGGGGCTATGTTACCGGAAGATGTCAAAGCCCGGGTAGGTGCCGTGGTCGAAGC
AGTAAAAGTACCTGTTGGGTTCCACGCCCACAACAACCTGACCATGGCTACGGCCAATGCCCTGGCGGCGGTAGAGGCTG
GCGCGACTTTCCTGGACGGTGCCTGTCGTGGCCTGGGGGCCGGAGCCGGCAATGCCCAAACAGAAGCTTTAGTCGGTGTG
CTTGACAAGCTGGGCTACCGGACGGGTGTCGATTTTTACAAAGTCATGGACGTAGCCGAAGACATCGTCGAGCCTATCAT
GCACCGGCCCCAGGTGGTGCGTAACGCACCCTTAATGCTGGGCTACGCCGGTGTTTACTCCAGTTTCTTACTCCACACCT
ACCGGGCGGCCGAGAAATTCAACCTCGACCCCCGGGACATCCTGGTGGAACTGGGAAGGAGGCGCATGGTCGGCGGGCAG
GAAGACATGATTGTCGATGTGGCTTACCAGTTAGCACAAAAGAGAGGAGGAAACTAA

Upstream 100 bases:

>100_bases
TCCTGCCCAAATACTCCGGTAATCTGGATATTATCACTTCAGCAGCCGTGGCGGTAGCTGAAAAGCTGGCCCAGGAACTG
CAGGTAAAGGAAGGGGTAGC

Downstream 100 bases:

>100_bases
ACAGTGAAACTAGTTACTCTGTTAGCGCAGGTAGATACTGACAAGTGTAAAGGTTGCCGTACCTGCGAACGGGTTTGCCC
GGTCCTGGCCATCAAGGTCG

Product: 4-hydroxy-2-ketovalerate aldolase

Products: NA

Alternate protein names: HOA; 4-hydroxy-2-keto-pentanoic acid aldolase; 4-hydroxy-2-oxopentanoate aldolase

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MSARSIHIVDTTLRDGSHAVSHQFTAEQIAAIAGGLDAAGVEYIEVSHGDGLAGSSYNYGWAALGDEEMLKAASAAIKKG
KLTVLLLPGIGTVEDLKMAADCGAKVVRVATHVTEADIGEQHIGMAKKLGMMAVGFLMMCHMAPPEKVVEQAKLFESYGA
DYINIADSAGAMLPEDVKARVGAVVEAVKVPVGFHAHNNLTMATANALAAVEAGATFLDGACRGLGAGAGNAQTEALVGV
LDKLGYRTGVDFYKVMDVAEDIVEPIMHRPQVVRNAPLMLGYAGVYSSFLLHTYRAAEKFNLDPRDILVELGRRRMVGGQ
EDMIVDVAYQLAQKRGGN

Sequences:

>Translated_338_residues
MSARSIHIVDTTLRDGSHAVSHQFTAEQIAAIAGGLDAAGVEYIEVSHGDGLAGSSYNYGWAALGDEEMLKAASAAIKKG
KLTVLLLPGIGTVEDLKMAADCGAKVVRVATHVTEADIGEQHIGMAKKLGMMAVGFLMMCHMAPPEKVVEQAKLFESYGA
DYINIADSAGAMLPEDVKARVGAVVEAVKVPVGFHAHNNLTMATANALAAVEAGATFLDGACRGLGAGAGNAQTEALVGV
LDKLGYRTGVDFYKVMDVAEDIVEPIMHRPQVVRNAPLMLGYAGVYSSFLLHTYRAAEKFNLDPRDILVELGRRRMVGGQ
EDMIVDVAYQLAQKRGGN
>Mature_337_residues
SARSIHIVDTTLRDGSHAVSHQFTAEQIAAIAGGLDAAGVEYIEVSHGDGLAGSSYNYGWAALGDEEMLKAASAAIKKGK
LTVLLLPGIGTVEDLKMAADCGAKVVRVATHVTEADIGEQHIGMAKKLGMMAVGFLMMCHMAPPEKVVEQAKLFESYGAD
YINIADSAGAMLPEDVKARVGAVVEAVKVPVGFHAHNNLTMATANALAAVEAGATFLDGACRGLGAGAGNAQTEALVGVL
DKLGYRTGVDFYKVMDVAEDIVEPIMHRPQVVRNAPLMLGYAGVYSSFLLHTYRAAEKFNLDPRDILVELGRRRMVGGQE
DMIVDVAYQLAQKRGGN

Specific function: Catalyzes the retro-aldol cleavage of 4-hydroxy-2- oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta-cleavage pathway for the degradation of aromatic compounds

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 4-hydroxy-2-oxovalerate aldolase family

Homologues:

Organism=Homo sapiens, GI109150422, Length=162, Percent_Identity=29.6296296296296, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI109150427, Length=162, Percent_Identity=29.6296296296296, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1786548, Length=337, Percent_Identity=52.8189910979229, Blast_Score=331, Evalue=3e-92,
Organism=Escherichia coli, GI1786261, Length=294, Percent_Identity=24.8299319727891, Blast_Score=75, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HOA_MOOTA (Q2RHL3)

Other databases:

- EMBL:   CP000232
- RefSeq:   YP_430619.1
- HSSP:   P35914
- ProteinModelPortal:   Q2RHL3
- SMR:   Q2RHL3
- STRING:   Q2RHL3
- GeneID:   3832441
- GenomeReviews:   CP000232_GR
- KEGG:   mta:Moth_1775
- NMPDR:   fig|264732.9.peg.1782
- eggNOG:   COG0119
- HOGENOM:   HBG300122
- OMA:   ELGSDAQ
- ProtClustDB:   PRK08195
- BioCyc:   MTHE264732:MOTH_1775-MONOMER
- HAMAP:   MF_01656
- InterPro:   IPR017629
- InterPro:   IPR013785
- InterPro:   IPR012425
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- ProDom:   PD005364
- TIGRFAMs:   TIGR03217

Pfam domain/function: PF07836 DmpG_comm; PF00682 HMGL-like

EC number: =4.1.3.39

Molecular weight: Translated: 35715; Mature: 35583

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS50991 PYR_CT

Important sites: BINDING 168-168 BINDING 195-195 BINDING 286-286

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSARSIHIVDTTLRDGSHAVSHQFTAEQIAAIAGGLDAAGVEYIEVSHGDGLAGSSYNYG
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCC
WAALGDEEMLKAASAAIKKGKLTVLLLPGIGTVEDLKMAADCGAKVVRVATHVTEADIGE
EEECCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCH
QHIGMAKKLGMMAVGFLMMCHMAPPEKVVEQAKLFESYGADYINIADSAGAMLPEDVKAR
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHH
VGAVVEAVKVPVGFHAHNNLTMATANALAAVEAGATFLDGACRGLGAGAGNAQTEALVGV
HHHHHHHHHCCCCEECCCCEEEEHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHH
LDKLGYRTGVDFYKVMDVAEDIVEPIMHRPQVVRNAPLMLGYAGVYSSFLLHTYRAAEKF
HHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEHHHHHHHHHHHHHHHHHHC
NLDPRDILVELGRRRMVGGQEDMIVDVAYQLAQKRGGN
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SARSIHIVDTTLRDGSHAVSHQFTAEQIAAIAGGLDAAGVEYIEVSHGDGLAGSSYNYG
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCC
WAALGDEEMLKAASAAIKKGKLTVLLLPGIGTVEDLKMAADCGAKVVRVATHVTEADIGE
EEECCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCH
QHIGMAKKLGMMAVGFLMMCHMAPPEKVVEQAKLFESYGADYINIADSAGAMLPEDVKAR
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHH
VGAVVEAVKVPVGFHAHNNLTMATANALAAVEAGATFLDGACRGLGAGAGNAQTEALVGV
HHHHHHHHHCCCCEECCCCEEEEHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHH
LDKLGYRTGVDFYKVMDVAEDIVEPIMHRPQVVRNAPLMLGYAGVYSSFLLHTYRAAEKF
HHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEHHHHHHHHHHHHHHHHHHC
NLDPRDILVELGRRRMVGGQEDMIVDVAYQLAQKRGGN
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA