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The map label for this gene is lpdA [H]

Identifier: 83590598

GI number: 83590598

Start: 1817596

End: 1818975

Strand: Reverse

Name: lpdA [H]

Synonym: Moth_1763

Alternate gene names: 83590598

Gene position: 1818975-1817596 (Counterclockwise)

Preceding gene: 83590599

Following gene: 83590597

Centisome position: 69.19

GC content: 61.45

Gene sequence:

>1380_bases
ATGAGTTACCAGATAGCCATCATCGGCGGTGGCCCCGGCGGTTACGTGGCGGCCATCCGGGCAGCCCAGCTGGGGGCGAA
GGTAGTCGTAATCGAACAGGACGCCCTGGGCGGCACCTGCCTCAACCGCGGCTGCATCCCCACCAAAGCCCTGCTGGCCG
GGGCGGCCATGGTCAGGGGCATCAAAGGGGCGGCTGCTTTTGGCATAGATGTAGAGGATTACCGGGTAGATTATGCCCGC
CTGGCGGCGCGTAAGGACGCCGTGGTCAAGCAGCTTACCGGGGGCATCGCCTACTTATTTAAGAAAAACAAGGTCGACCT
CATAAAAGGGCGGGGCTTTCTTAAAGGTCCTGGCCAGATCGAAGTGGCTACGGCCGATGGCACGATAGAAAACCTCCAGG
CCGAAAACATCATCCTGGCCACCGGGTCGGAGCCGGCCCTGATTAAGGCCCTGGGATATAACGGCCGCACCGTGGTCACC
AGCACCGAGGCCCTGGCCTGGACGGAGGTGCCGGCCGAGCTCCTGATTATCGGCGGCGGGGTCATCGGCTGCGAGTTTGC
CACCCTCTTTGCCACTCTGGGGAGCAAGGTCACCATCGTCGAAATGATGCCCGCCATCTTACCCATGATCGACAGTGAGA
TCTCCAGGCGCTTCAGTATGCTCCTCAAAAAGACCGGGGTGGAAATAAAGACAAAGGCCCAGATAACAGAAGTCAAGGAA
GCCGGCGGCCGGGTCCAGGCCACCCTTGCCGACGGCCAGACTATAAACGCCGATAAGGTTCTTATCTCCATCGGCCGCCA
GTTCAATACCCGGGGCTTAGGCCTGGAAGATGCCGGCATAACCCTGGGTCCCAAGGGTGAAATCGTAGTTGACGAATACC
TGCGGACCAGCGTCCCCGGCATCTACGCCATCGGCGACGTGACCAACAAGATCCAGCTGGCCCACGTAGCTTCGGCCCAG
GGCCTGGCGGCGGTGACAACCATCATGGGCCGGCCGACGAAGGTGAACTACGACGCCGTACCTAGCTGCATCTACACCCT
GCCGGAGATAGCCGGCGTCGGCCTGACTAAAGAGGCGGCCGAAGGGCGGGGCATGAAGGTTAGGGTCGGCAAATTCCCCT
TCCAGGCCAGCGGCAAGGCCCTCTGCAGCGGCGAGACTGACGGCATGGTCAAGATAATCGCCGAGGCAGAGAGCGACCGG
GTGGTTGGGGTCTTCATCATGGGTCCCCACGCCACGGAACTCATTGCCGAGGGTGCCCTGGCAGTCAATAAAGGAATAAC
GGCCGGCGAACTGGCGGCGACCATTCACGCCCATCCCACCCTGTCCGAGGCCGTTATGGAGGCCGCGGAGGCCGTACACG
GGCTTAGTATCCATTCATAG

Upstream 100 bases:

>100_bases
ATGGACCGGGTTAGGTGGAGCCATTGTTACCTTGCACGGCTCCGGTGGGTGTTTTTACAGGCTGGGATCGGCTAAAGTAA
TTGGTTTGGAGGGGTTTTCC

Downstream 100 bases:

>100_bases
GGGCTGGAGGGTACAGGCGAGGGCGACTCGTTTCGCGACGTAAATAAACTTTGCGCGCAGCCGATAGCTTCGCGGCGGTG
GCTTAAGCAACTTAAGCGCC

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MSYQIAIIGGGPGGYVAAIRAAQLGAKVVVIEQDALGGTCLNRGCIPTKALLAGAAMVRGIKGAAAFGIDVEDYRVDYAR
LAARKDAVVKQLTGGIAYLFKKNKVDLIKGRGFLKGPGQIEVATADGTIENLQAENIILATGSEPALIKALGYNGRTVVT
STEALAWTEVPAELLIIGGGVIGCEFATLFATLGSKVTIVEMMPAILPMIDSEISRRFSMLLKKTGVEIKTKAQITEVKE
AGGRVQATLADGQTINADKVLISIGRQFNTRGLGLEDAGITLGPKGEIVVDEYLRTSVPGIYAIGDVTNKIQLAHVASAQ
GLAAVTTIMGRPTKVNYDAVPSCIYTLPEIAGVGLTKEAAEGRGMKVRVGKFPFQASGKALCSGETDGMVKIIAEAESDR
VVGVFIMGPHATELIAEGALAVNKGITAGELAATIHAHPTLSEAVMEAAEAVHGLSIHS

Sequences:

>Translated_459_residues
MSYQIAIIGGGPGGYVAAIRAAQLGAKVVVIEQDALGGTCLNRGCIPTKALLAGAAMVRGIKGAAAFGIDVEDYRVDYAR
LAARKDAVVKQLTGGIAYLFKKNKVDLIKGRGFLKGPGQIEVATADGTIENLQAENIILATGSEPALIKALGYNGRTVVT
STEALAWTEVPAELLIIGGGVIGCEFATLFATLGSKVTIVEMMPAILPMIDSEISRRFSMLLKKTGVEIKTKAQITEVKE
AGGRVQATLADGQTINADKVLISIGRQFNTRGLGLEDAGITLGPKGEIVVDEYLRTSVPGIYAIGDVTNKIQLAHVASAQ
GLAAVTTIMGRPTKVNYDAVPSCIYTLPEIAGVGLTKEAAEGRGMKVRVGKFPFQASGKALCSGETDGMVKIIAEAESDR
VVGVFIMGPHATELIAEGALAVNKGITAGELAATIHAHPTLSEAVMEAAEAVHGLSIHS
>Mature_458_residues
SYQIAIIGGGPGGYVAAIRAAQLGAKVVVIEQDALGGTCLNRGCIPTKALLAGAAMVRGIKGAAAFGIDVEDYRVDYARL
AARKDAVVKQLTGGIAYLFKKNKVDLIKGRGFLKGPGQIEVATADGTIENLQAENIILATGSEPALIKALGYNGRTVVTS
TEALAWTEVPAELLIIGGGVIGCEFATLFATLGSKVTIVEMMPAILPMIDSEISRRFSMLLKKTGVEIKTKAQITEVKEA
GGRVQATLADGQTINADKVLISIGRQFNTRGLGLEDAGITLGPKGEIVVDEYLRTSVPGIYAIGDVTNKIQLAHVASAQG
LAAVTTIMGRPTKVNYDAVPSCIYTLPEIAGVGLTKEAAEGRGMKVRVGKFPFQASGKALCSGETDGMVKIIAEAESDRV
VGVFIMGPHATELIAEGALAVNKGITAGELAATIHAHPTLSEAVMEAAEAVHGLSIHS

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=456, Percent_Identity=42.5438596491228, Blast_Score=352, Evalue=5e-97,
Organism=Homo sapiens, GI50301238, Length=461, Percent_Identity=31.236442516269, Blast_Score=189, Evalue=4e-48,
Organism=Homo sapiens, GI33519430, Length=451, Percent_Identity=29.0465631929047, Blast_Score=149, Evalue=7e-36,
Organism=Homo sapiens, GI33519428, Length=451, Percent_Identity=29.0465631929047, Blast_Score=149, Evalue=7e-36,
Organism=Homo sapiens, GI33519426, Length=451, Percent_Identity=29.0465631929047, Blast_Score=149, Evalue=7e-36,
Organism=Homo sapiens, GI148277065, Length=454, Percent_Identity=28.8546255506608, Blast_Score=148, Evalue=8e-36,
Organism=Homo sapiens, GI148277071, Length=454, Percent_Identity=28.8546255506608, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI22035672, Length=464, Percent_Identity=29.9568965517241, Blast_Score=139, Evalue=8e-33,
Organism=Homo sapiens, GI291045266, Length=457, Percent_Identity=26.6958424507659, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI291045268, Length=448, Percent_Identity=24.7767857142857, Blast_Score=108, Evalue=8e-24,
Organism=Escherichia coli, GI1786307, Length=456, Percent_Identity=39.4736842105263, Blast_Score=303, Evalue=1e-83,
Organism=Escherichia coli, GI87082354, Length=453, Percent_Identity=28.9183222958057, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI87081717, Length=451, Percent_Identity=30.820399113082, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI1789915, Length=432, Percent_Identity=29.1666666666667, Blast_Score=168, Evalue=8e-43,
Organism=Escherichia coli, GI1789065, Length=269, Percent_Identity=27.1375464684015, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI32565766, Length=457, Percent_Identity=41.1378555798687, Blast_Score=339, Evalue=2e-93,
Organism=Caenorhabditis elegans, GI17557007, Length=475, Percent_Identity=28.4210526315789, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI71983429, Length=434, Percent_Identity=28.5714285714286, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI71983419, Length=434, Percent_Identity=28.5714285714286, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI71982272, Length=476, Percent_Identity=24.1596638655462, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17559934, Length=230, Percent_Identity=25.6521739130435, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6321091, Length=473, Percent_Identity=40.8033826638478, Blast_Score=323, Evalue=4e-89,
Organism=Saccharomyces cerevisiae, GI6325166, Length=460, Percent_Identity=30.2173913043478, Blast_Score=202, Evalue=6e-53,
Organism=Saccharomyces cerevisiae, GI6325240, Length=471, Percent_Identity=27.6008492569002, Blast_Score=166, Evalue=5e-42,
Organism=Drosophila melanogaster, GI21358499, Length=454, Percent_Identity=41.8502202643172, Blast_Score=343, Evalue=2e-94,
Organism=Drosophila melanogaster, GI17737741, Length=472, Percent_Identity=27.3305084745763, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24640549, Length=480, Percent_Identity=26.875, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24640553, Length=480, Percent_Identity=26.875, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24640551, Length=480, Percent_Identity=26.875, Blast_Score=121, Evalue=1e-27,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 47648; Mature: 47517

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYQIAIIGGGPGGYVAAIRAAQLGAKVVVIEQDALGGTCLNRGCIPTKALLAGAAMVRG
CCEEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHCCCCCCHHHHHHHHHHHHC
IKGAAAFGIDVEDYRVDYARLAARKDAVVKQLTGGIAYLFKKNKVDLIKGRGFLKGPGQI
CCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEEEECCCEECCCCEE
EVATADGTIENLQAENIILATGSEPALIKALGYNGRTVVTSTEALAWTEVPAELLIIGGG
EEEECCCCHHCCCCCEEEEEECCCCEEEEEECCCCEEEEEECCCEEEECCCEEEEEEECC
VIGCEFATLFATLGSKVTIVEMMPAILPMIDSEISRRFSMLLKKTGVEIKTKAQITEVKE
CHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHH
AGGRVQATLADGQTINADKVLISIGRQFNTRGLGLEDAGITLGPKGEIVVDEYLRTSVPG
CCCEEEEEECCCCEECCCCEEEEECCCCCCCCCCCCCCCEEECCCCCEEEHHHHHHCCCC
IYAIGDVTNKIQLAHVASAQGLAAVTTIMGRPTKVNYDAVPSCIYTLPEIAGVGLTKEAA
EEEECCCCCEEEEEEECCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHH
EGRGMKVRVGKFPFQASGKALCSGETDGMVKIIAEAESDRVVGVFIMGPHATELIAEGAL
CCCCEEEEEECCCCCCCCCEEECCCCCCEEEEEEECCCCCEEEEEEECCCHHHHHHCCCE
AVNKGITAGELAATIHAHPTLSEAVMEAAEAVHGLSIHS
EECCCCCHHHEEEEEECCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SYQIAIIGGGPGGYVAAIRAAQLGAKVVVIEQDALGGTCLNRGCIPTKALLAGAAMVRG
CEEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHCCCCCCHHHHHHHHHHHHC
IKGAAAFGIDVEDYRVDYARLAARKDAVVKQLTGGIAYLFKKNKVDLIKGRGFLKGPGQI
CCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEEEECCCEECCCCEE
EVATADGTIENLQAENIILATGSEPALIKALGYNGRTVVTSTEALAWTEVPAELLIIGGG
EEEECCCCHHCCCCCEEEEEECCCCEEEEEECCCCEEEEEECCCEEEECCCEEEEEEECC
VIGCEFATLFATLGSKVTIVEMMPAILPMIDSEISRRFSMLLKKTGVEIKTKAQITEVKE
CHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHH
AGGRVQATLADGQTINADKVLISIGRQFNTRGLGLEDAGITLGPKGEIVVDEYLRTSVPG
CCCEEEEEECCCCEECCCCEEEEECCCCCCCCCCCCCCCEEECCCCCEEEHHHHHHCCCC
IYAIGDVTNKIQLAHVASAQGLAAVTTIMGRPTKVNYDAVPSCIYTLPEIAGVGLTKEAA
EEEECCCCCEEEEEEECCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHH
EGRGMKVRVGKFPFQASGKALCSGETDGMVKIIAEAESDRVVGVFIMGPHATELIAEGAL
CCCCEEEEEECCCCCCCCCEEECCCCCCEEEEEEECCCCCEEEEEEECCCHHHHHHCCCE
AVNKGITAGELAATIHAHPTLSEAVMEAAEAVHGLSIHS
EECCCCCHHHEEEEEECCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]