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The map label for this gene is lipA

Identifier: 83590597

GI number: 83590597

Start: 1816500

End: 1817390

Strand: Reverse

Name: lipA

Synonym: Moth_1762

Alternate gene names: 83590597

Gene position: 1817390-1816500 (Counterclockwise)

Preceding gene: 83590598

Following gene: 83590596

Centisome position: 69.13

GC content: 62.51

Gene sequence:

>891_bases
ATGGTTACTTCACCTTCAGGAACAGGGACGCGGCGGATGCCGCCCTGGCTCCATAAACGCCTGCCGGCTTCCGGGGATAT
AGAGGCCACCCGGCGGCTTCTGGCCGACCTGCACCTTAACACCGTCTGCCAGAGCGCTCGCTGCCCCAACCAGGGGGAGT
GTTTCGCCAGCAGGACGGCCACCTTTATGATCTTGGGCAACACCTGTACCCGCAACTGCCGCTTCTGCGCCGTGGAACAC
GGCCAGCCGGAGGCCATTGATGAGGACGAACCCCGGCGGGTGGCCGAAGCAGCCCGGCGCCTGGGGCTCAAGCACGTCGT
CGTTACCTCCGTCACCCGGGACGACCTGCCCGACGGTGGCGCCGGCCACTTTGCCGCCACCATTACTGCCCTGCGCCAGG
CCCTGCCGGGGGCCTATATCGAGGTCCTCACCCCGGATTTCCGTGGTAGTCGTGCTGCCCTGGCCGCCGTCGCCCGGGCC
AGGCCGGATATCTTTAACCATAACGTTGAGACGGTGCCCCGTCTTTACCCGGATGTCCGGCCCCAGGCCGATTACCGCCG
CAGCCTTGACGTTCTAAAACAAATGAAGGAACTGGACGCGACGATCTATACAAAGTCCGGCCTGATGGTCGGCGTGGGGG
AAAGCAGGGAAGAGGTCCTGGCAGTAATGGCCGACCTGCGGGCTGTCAACTGTGACATCCTGACCATCGGCCAGTACCTG
CGGCCATCCCCCCGGCACTTGGAAATCAAAGAGTATGTCCCTCCAGAGACCTTTACCTGGTATGCCCAAAAAGGCCGGGA
GATGGGCTTTCTCTATGTGGCTGCGGGACCCTATGTCCGCAGCTCTTATCACGCCGCTGAGTTTAGCGTGAAGATAGCCG
GGGTAAAGTAA

Upstream 100 bases:

>100_bases
TGCTAGCTTAAGCACCCTGAGCCCAGAGACTGCATCTTAACCCCTCAGAAGCCAACAAATAATCGCCTGATTTGGTAGGA
CCCTTTTTCGGAGGAAAACC

Downstream 100 bases:

>100_bases
AGGGCGGCCACAGCGCATACCATACCTAAAAACAGGGCATCCCCTTGACAAGACCGCCCTTTTCGGGCATAATATATGGT
GTCCAGCAGAAAGGGCTCGT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MVTSPSGTGTRRMPPWLHKRLPASGDIEATRRLLADLHLNTVCQSARCPNQGECFASRTATFMILGNTCTRNCRFCAVEH
GQPEAIDEDEPRRVAEAARRLGLKHVVVTSVTRDDLPDGGAGHFAATITALRQALPGAYIEVLTPDFRGSRAALAAVARA
RPDIFNHNVETVPRLYPDVRPQADYRRSLDVLKQMKELDATIYTKSGLMVGVGESREEVLAVMADLRAVNCDILTIGQYL
RPSPRHLEIKEYVPPETFTWYAQKGREMGFLYVAAGPYVRSSYHAAEFSVKIAGVK

Sequences:

>Translated_296_residues
MVTSPSGTGTRRMPPWLHKRLPASGDIEATRRLLADLHLNTVCQSARCPNQGECFASRTATFMILGNTCTRNCRFCAVEH
GQPEAIDEDEPRRVAEAARRLGLKHVVVTSVTRDDLPDGGAGHFAATITALRQALPGAYIEVLTPDFRGSRAALAAVARA
RPDIFNHNVETVPRLYPDVRPQADYRRSLDVLKQMKELDATIYTKSGLMVGVGESREEVLAVMADLRAVNCDILTIGQYL
RPSPRHLEIKEYVPPETFTWYAQKGREMGFLYVAAGPYVRSSYHAAEFSVKIAGVK
>Mature_296_residues
MVTSPSGTGTRRMPPWLHKRLPASGDIEATRRLLADLHLNTVCQSARCPNQGECFASRTATFMILGNTCTRNCRFCAVEH
GQPEAIDEDEPRRVAEAARRLGLKHVVVTSVTRDDLPDGGAGHFAATITALRQALPGAYIEVLTPDFRGSRAALAAVARA
RPDIFNHNVETVPRLYPDVRPQADYRRSLDVLKQMKELDATIYTKSGLMVGVGESREEVLAVMADLRAVNCDILTIGQYL
RPSPRHLEIKEYVPPETFTWYAQKGREMGFLYVAAGPYVRSSYHAAEFSVKIAGVK

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=287, Percent_Identity=46.6898954703833, Blast_Score=280, Evalue=9e-76,
Organism=Homo sapiens, GI37577164, Length=245, Percent_Identity=45.7142857142857, Blast_Score=230, Evalue=9e-61,
Organism=Escherichia coli, GI1786846, Length=276, Percent_Identity=50.7246376811594, Blast_Score=275, Evalue=3e-75,
Organism=Caenorhabditis elegans, GI32564533, Length=290, Percent_Identity=45.8620689655172, Blast_Score=266, Evalue=1e-71,
Organism=Saccharomyces cerevisiae, GI6324770, Length=284, Percent_Identity=44.3661971830986, Blast_Score=266, Evalue=2e-72,
Organism=Drosophila melanogaster, GI221513272, Length=286, Percent_Identity=43.3566433566434, Blast_Score=267, Evalue=6e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_MOOTA (Q2RHM6)

Other databases:

- EMBL:   CP000232
- RefSeq:   YP_430606.1
- ProteinModelPortal:   Q2RHM6
- STRING:   Q2RHM6
- GeneID:   3831054
- GenomeReviews:   CP000232_GR
- KEGG:   mta:Moth_1762
- NMPDR:   fig|264732.9.peg.1770
- eggNOG:   COG0320
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- PhylomeDB:   Q2RHM6
- ProtClustDB:   PRK05481
- BioCyc:   MTHE264732:MOTH_1762-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 32739; Mature: 32739

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTSPSGTGTRRMPPWLHKRLPASGDIEATRRLLADLHLNTVCQSARCPNQGECFASRTA
CCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCE
TFMILGNTCTRNCRFCAVEHGQPEAIDEDEPRRVAEAARRLGLKHVVVTSVTRDDLPDGG
EEEEECCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECHHCCCCCCC
AGHFAATITALRQALPGAYIEVLTPDFRGSRAALAAVARARPDIFNHNVETVPRLYPDVR
CCHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCHHHCCCHHHHHHHCCCCC
PQADYRRSLDVLKQMKELDATIYTKSGLMVGVGESREEVLAVMADLRAVNCDILTIGQYL
CCHHHHHHHHHHHHHHHHCCEEEECCCEEEECCCCHHHHHHHHHHHHHCCCCEEECCHHH
RPSPRHLEIKEYVPPETFTWYAQKGREMGFLYVAAGPYVRSSYHAAEFSVKIAGVK
CCCCCCEEEHHCCCCCCEEHHHHCCCCEEEEEEECCCHHHCCCCEEEEEEEEECCC
>Mature Secondary Structure
MVTSPSGTGTRRMPPWLHKRLPASGDIEATRRLLADLHLNTVCQSARCPNQGECFASRTA
CCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCE
TFMILGNTCTRNCRFCAVEHGQPEAIDEDEPRRVAEAARRLGLKHVVVTSVTRDDLPDGG
EEEEECCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECHHCCCCCCC
AGHFAATITALRQALPGAYIEVLTPDFRGSRAALAAVARARPDIFNHNVETVPRLYPDVR
CCHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCHHHCCCHHHHHHHCCCCC
PQADYRRSLDVLKQMKELDATIYTKSGLMVGVGESREEVLAVMADLRAVNCDILTIGQYL
CCHHHHHHHHHHHHHHHHCCEEEECCCEEEECCCCHHHHHHHHHHHHHCCCCEEECCHHH
RPSPRHLEIKEYVPPETFTWYAQKGREMGFLYVAAGPYVRSSYHAAEFSVKIAGVK
CCCCCCEEEHHCCCCCCEEHHHHCCCCEEEEEEECCCHHHCCCCEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA