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The map label for this gene is ytzG [H]

Identifier: 83590579

GI number: 83590579

Start: 1796213

End: 1796944

Strand: Reverse

Name: ytzG [H]

Synonym: Moth_1744

Alternate gene names: 83590579

Gene position: 1796944-1796213 (Counterclockwise)

Preceding gene: 83590580

Following gene: 83590574

Centisome position: 68.36

GC content: 50.14

Gene sequence:

>732_bases
ATGGCGGCAAAGGAGCGGCAGCGCCTGGATAGAGTGCTGGCCCACATGGGGTTTGGTACGCGTAAAATGATTAAGAAGCT
GATAAAAGAGAAAAGAGTGCAGGTCAACGGCAGGCCGGCTGAGGACCCAGGGTTACATGTTATCCCAGGCCGGGATTACA
TTGAGGTGAACGGCGAACCCCTGGTGTATGAAGAGTACATCTATTTAATGTTAAATAAGCCGCAGGGTGTCCTCTCCGCT
ACAGAGGATAGATATGCCAAAGTAGTAGTCGACCTGCTTCCTCCCCGGTATCGTGCCTTTCATCCCTTCCCGGTAGGGAG
ACTGGACAAAGATGCCGAGGGGCTGCTTTTGCTCACCAATGACGGCGAACTGGCCCACCGGCTTCTATCGCCCAAAAAAC
ATGTGTCCAAAATGTATTACGCTGTGGTAGACGGTATAGTTACGGAAACAGACGTAGAAGCGTTCCGCCAGGGGATAATC
TTGAATGACGGCTATCGAACCATGCCTGGCGAGTTAACGATCCTCCGCTCCGGAACGGAATCGGAGGTTGAGCTCACCAT
TTATGAAGGAAAATATCATCAGGTCAAACGCATGTTTGAGGCTGTAAGTAAAAGAGTGCTATATCTTAAACGAATCGCTA
TGGGACCCTTAAGGCTGGACGAACTTTTAAGGCCCGGCGAGTACCGGGAATTGACGGAAGAAGAAATCATCAAGCTGCGG
GAATTACTTTAA

Upstream 100 bases:

>100_bases
CTGCCGCGGGGGAGCTACGGGACAATGCTCATCAAAAGGCTGACCATGCCTTGAACCGGGATGGCATCCGGCGGGATAGG
GAAGGGTGATGAGGTAAAAA

Downstream 100 bases:

>100_bases
ATGCCGGCAGGGAAGGCAAATCCTTCCGGGAGCTGCCCGACCCCACCTGGGGGGTAGCTCCCGGGGATTTTTAAGGGGTA
AAGGGGTCAAAGAACCGGCC

Product: ribosomal small subunit pseudouridine synthase A

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MAAKERQRLDRVLAHMGFGTRKMIKKLIKEKRVQVNGRPAEDPGLHVIPGRDYIEVNGEPLVYEEYIYLMLNKPQGVLSA
TEDRYAKVVVDLLPPRYRAFHPFPVGRLDKDAEGLLLLTNDGELAHRLLSPKKHVSKMYYAVVDGIVTETDVEAFRQGII
LNDGYRTMPGELTILRSGTESEVELTIYEGKYHQVKRMFEAVSKRVLYLKRIAMGPLRLDELLRPGEYRELTEEEIIKLR
ELL

Sequences:

>Translated_243_residues
MAAKERQRLDRVLAHMGFGTRKMIKKLIKEKRVQVNGRPAEDPGLHVIPGRDYIEVNGEPLVYEEYIYLMLNKPQGVLSA
TEDRYAKVVVDLLPPRYRAFHPFPVGRLDKDAEGLLLLTNDGELAHRLLSPKKHVSKMYYAVVDGIVTETDVEAFRQGII
LNDGYRTMPGELTILRSGTESEVELTIYEGKYHQVKRMFEAVSKRVLYLKRIAMGPLRLDELLRPGEYRELTEEEIIKLR
ELL
>Mature_242_residues
AAKERQRLDRVLAHMGFGTRKMIKKLIKEKRVQVNGRPAEDPGLHVIPGRDYIEVNGEPLVYEEYIYLMLNKPQGVLSAT
EDRYAKVVVDLLPPRYRAFHPFPVGRLDKDAEGLLLLTNDGELAHRLLSPKKHVSKMYYAVVDGIVTETDVEAFRQGIIL
NDGYRTMPGELTILRSGTESEVELTIYEGKYHQVKRMFEAVSKRVLYLKRIAMGPLRLDELLRPGEYRELTEEEIIKLRE
LL

Specific function: Responsible For Synthesis Of Pseudouridine From Uracil-516 In 16s Ribosomal RNA. [C]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=235, Percent_Identity=38.7234042553192, Blast_Score=146, Evalue=1e-36,
Organism=Escherichia coli, GI87081838, Length=182, Percent_Identity=37.3626373626374, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1790453, Length=242, Percent_Identity=31.404958677686, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787524, Length=246, Percent_Identity=33.3333333333333, Blast_Score=102, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70

Molecular weight: Translated: 28005; Mature: 27874

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKERQRLDRVLAHMGFGTRKMIKKLIKEKRVQVNGRPAEDPGLHVIPGRDYIEVNGEP
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCEEECCCCCCCCCEEEECCCCEEEECCCC
LVYEEYIYLMLNKPQGVLSATEDRYAKVVVDLLPPRYRAFHPFPVGRLDKDAEGLLLLTN
EEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEC
DGELAHRLLSPKKHVSKMYYAVVDGIVTETDVEAFRQGIILNDGYRTMPGELTILRSGTE
CHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHHCCCEEECCCEECCCCEEEEECCCC
SEVELTIYEGKYHQVKRMFEAVSKRVLYLKRIAMGPLRLDELLRPGEYRELTEEEIIKLR
CEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHCCHHHHHHHH
ELL
HCC
>Mature Secondary Structure 
AAKERQRLDRVLAHMGFGTRKMIKKLIKEKRVQVNGRPAEDPGLHVIPGRDYIEVNGEP
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCEEECCCCCCCCCEEEECCCCEEEECCCC
LVYEEYIYLMLNKPQGVLSATEDRYAKVVVDLLPPRYRAFHPFPVGRLDKDAEGLLLLTN
EEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEC
DGELAHRLLSPKKHVSKMYYAVVDGIVTETDVEAFRQGIILNDGYRTMPGELTILRSGTE
CHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHHHHHCCCEEECCCEECCCCEEEEECCCC
SEVELTIYEGKYHQVKRMFEAVSKRVLYLKRIAMGPLRLDELLRPGEYRELTEEEIIKLR
CEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHCCHHHHHHHH
ELL
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]