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The map label for this gene is queA

Identifier: 83590531

GI number: 83590531

Start: 1732669

End: 1733697

Strand: Reverse

Name: queA

Synonym: Moth_1695

Alternate gene names: 83590531

Gene position: 1733697-1732669 (Counterclockwise)

Preceding gene: 83590532

Following gene: 83590530

Centisome position: 65.95

GC content: 63.07

Gene sequence:

>1029_bases
TTGCGGATAGAAGAATTTGACTACGAGTTGCCGCCAGGACAAATCGCCCAGCAACCGGTGGAGCCGCGGGATGCCTCGCG
GCTCCTCGTCCTGCACCGGGAAGGTGGTTTCCTGGAGCACCGTCACTTCTACGACCTGCCACGCTACCTCCACCCCGGCG
ACGTGCTGGTGGTGAATGAGACGAAGGTAATTCCCGCCCGCCTGTGGGGCTACCGTGCCGGGACGGGCGCTAAAATCGAA
GTCCTCTTATTGACCCGGCAGGAAGGTGACACCTGGGAAACCCTGGTGCGGCCGGGACGACGGGTACCGGTGGGCGCGGA
GCTAATCTTCGGCCGGGGTGAATTGCAGGCCCGGGTAAAGGGGGTAACCCCGGCCGGAGGACGGATAATGGAGTTCTCTT
ATCAGGAGGGCCCCTGGGAGGCCTTGCTGGAACGCCTGGGGGAAATGCCCCTGCCCCCTTATATCAAAGAAAAGCCGGCT
GATCCCGGCCGTTACCAGACGGTTTACGCCCGGGAGGAGGGCTCGGCTGCCGCCCCTACCGCAGGCCTGCACTTTACCCC
CCGCCTCCTTAAGGAACTCCGGGAGCAGGGGATAGAAATAGCCAGCATCCTGCTCCACGTAGGCCTGGGGACCTTCCGGC
CGGTAAAGGTGGAGAACATCCAGGAACACGTGATGCACGCCGAATACTACGCCGTGACCCCGGCGGCGGCGGCGACCATC
AATGCCGCCCGGGCCCGGGGCCACCGGGTGGTTGCTGTAGGCACAACCGTCGTCCGCACCCTGGAGACGGTAGCTACAGC
CGATGGCGTCATCCATGCCGGCAGCGGCTGGACGGACATCTTTATCTATCCCGGTTACCGGTTCAAGGCCATCGACAGCC
TGATCACCAACTTTCACCTGCCCCGCTCGACCCTGTTGATGCTGGTCAGTGCCTTTGCCGGCAGGGAGAAGATCCTGGAC
GCCTACCGGGTCGCCGTCCGGGAAGGCTACCGCTTTTACAGTTTCGGCGACGCTATGTTGATCCTGTAA

Upstream 100 bases:

>100_bases
ACCAGCAAATTATCGAACACTACTACAACCAGGATCATTATGACGGCCACCTGAAGATTGCGACCTATTGATTTTAATAA
CAGACAGGTGAAGGGTTAAG

Downstream 100 bases:

>100_bases
AGAATTTATATGGTAAGAAAGCTAATTGGAGGAAGACATGCCTGCAGTAACCTTTACCGTACTTAAACGCGACCGGTCTA
CCGGGGCGCGGCTGGGGCGT

Product: S-adenosylmethionine

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MRIEEFDYELPPGQIAQQPVEPRDASRLLVLHREGGFLEHRHFYDLPRYLHPGDVLVVNETKVIPARLWGYRAGTGAKIE
VLLLTRQEGDTWETLVRPGRRVPVGAELIFGRGELQARVKGVTPAGGRIMEFSYQEGPWEALLERLGEMPLPPYIKEKPA
DPGRYQTVYAREEGSAAAPTAGLHFTPRLLKELREQGIEIASILLHVGLGTFRPVKVENIQEHVMHAEYYAVTPAAAATI
NAARARGHRVVAVGTTVVRTLETVATADGVIHAGSGWTDIFIYPGYRFKAIDSLITNFHLPRSTLLMLVSAFAGREKILD
AYRVAVREGYRFYSFGDAMLIL

Sequences:

>Translated_342_residues
MRIEEFDYELPPGQIAQQPVEPRDASRLLVLHREGGFLEHRHFYDLPRYLHPGDVLVVNETKVIPARLWGYRAGTGAKIE
VLLLTRQEGDTWETLVRPGRRVPVGAELIFGRGELQARVKGVTPAGGRIMEFSYQEGPWEALLERLGEMPLPPYIKEKPA
DPGRYQTVYAREEGSAAAPTAGLHFTPRLLKELREQGIEIASILLHVGLGTFRPVKVENIQEHVMHAEYYAVTPAAAATI
NAARARGHRVVAVGTTVVRTLETVATADGVIHAGSGWTDIFIYPGYRFKAIDSLITNFHLPRSTLLMLVSAFAGREKILD
AYRVAVREGYRFYSFGDAMLIL
>Mature_342_residues
MRIEEFDYELPPGQIAQQPVEPRDASRLLVLHREGGFLEHRHFYDLPRYLHPGDVLVVNETKVIPARLWGYRAGTGAKIE
VLLLTRQEGDTWETLVRPGRRVPVGAELIFGRGELQARVKGVTPAGGRIMEFSYQEGPWEALLERLGEMPLPPYIKEKPA
DPGRYQTVYAREEGSAAAPTAGLHFTPRLLKELREQGIEIASILLHVGLGTFRPVKVENIQEHVMHAEYYAVTPAAAATI
NAARARGHRVVAVGTTVVRTLETVATADGVIHAGSGWTDIFIYPGYRFKAIDSLITNFHLPRSTLLMLVSAFAGREKILD
AYRVAVREGYRFYSFGDAMLIL

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=346, Percent_Identity=47.1098265895954, Blast_Score=298, Evalue=5e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_MOOTA (Q2RHU2)

Other databases:

- EMBL:   CP000232
- RefSeq:   YP_430540.1
- HSSP:   O32054
- ProteinModelPortal:   Q2RHU2
- SMR:   Q2RHU2
- STRING:   Q2RHU2
- GeneID:   3833295
- GenomeReviews:   CP000232_GR
- KEGG:   mta:Moth_1695
- NMPDR:   fig|264732.9.peg.1694
- eggNOG:   COG0809
- HOGENOM:   HBG286076
- OMA:   SEFYRLT
- PhylomeDB:   Q2RHU2
- BioCyc:   MTHE264732:MOTH_1695-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38177; Mature: 38177

Theoretical pI: Translated: 8.01; Mature: 8.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIEEFDYELPPGQIAQQPVEPRDASRLLVLHREGGFLEHRHFYDLPRYLHPGDVLVVNE
CCCCCCCCCCCCCHHHCCCCCCCCCCEEEEEECCCCCCCCCHHHCCCCCCCCCCEEEEEC
TKVIPARLWGYRAGTGAKIEVLLLTRQEGDTWETLVRPGRRVPVGAELIFGRGELQARVK
CEEEEHHHCCEECCCCCEEEEEEEECCCCCHHHHHHCCCCCCCCCCEEEECCCCEEEEEE
GVTPAGGRIMEFSYQEGPWEALLERLGEMPLPPYIKEKPADPGRYQTVYAREEGSAAAPT
CCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCC
AGLHFTPRLLKELREQGIEIASILLHVGLGTFRPVKVENIQEHVMHAEYYAVTPAAAATI
CCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHEEECCHHHHHH
NAARARGHRVVAVGTTVVRTLETVATADGVIHAGSGWTDIFIYPGYRFKAIDSLITNFHL
HHHHHCCCEEEEEHHHHHHHHHHHHHCCCEEECCCCCEEEEEECCCHHHHHHHHHHHCCC
PRSTLLMLVSAFAGREKILDAYRVAVREGYRFYSFGDAMLIL
CHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEECCEEEEC
>Mature Secondary Structure
MRIEEFDYELPPGQIAQQPVEPRDASRLLVLHREGGFLEHRHFYDLPRYLHPGDVLVVNE
CCCCCCCCCCCCCHHHCCCCCCCCCCEEEEEECCCCCCCCCHHHCCCCCCCCCCEEEEEC
TKVIPARLWGYRAGTGAKIEVLLLTRQEGDTWETLVRPGRRVPVGAELIFGRGELQARVK
CEEEEHHHCCEECCCCCEEEEEEEECCCCCHHHHHHCCCCCCCCCCEEEECCCCEEEEEE
GVTPAGGRIMEFSYQEGPWEALLERLGEMPLPPYIKEKPADPGRYQTVYAREEGSAAAPT
CCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCC
AGLHFTPRLLKELREQGIEIASILLHVGLGTFRPVKVENIQEHVMHAEYYAVTPAAAATI
CCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHEEECCHHHHHH
NAARARGHRVVAVGTTVVRTLETVATADGVIHAGSGWTDIFIYPGYRFKAIDSLITNFHL
HHHHHCCCEEEEEHHHHHHHHHHHHHCCCEEECCCCCEEEEEECCCHHHHHHHHHHHCCC
PRSTLLMLVSAFAGREKILDAYRVAVREGYRFYSFGDAMLIL
CHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA