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The map label for this gene is tgt

Identifier: 83590530

GI number: 83590530

Start: 1731510

End: 1732631

Strand: Reverse

Name: tgt

Synonym: Moth_1694

Alternate gene names: 83590530

Gene position: 1732631-1731510 (Counterclockwise)

Preceding gene: 83590531

Following gene: 83590529

Centisome position: 65.91

GC content: 61.14

Gene sequence:

>1122_bases
ATGCCTGCAGTAACCTTTACCGTACTTAAACGCGACCGGTCTACCGGGGCGCGGCTGGGGCGTCTCACAACCCCCCACGG
CACCATCGAGACGCCGGTTTTCATGCCCGTGGGCACCCAGGCCACCGTCAAGACCATGACCCCGGAAGAGGTGGCCGGCC
TGGGAGCGGAGATCATCCTGGCCAACACCTATCATCTCTACCTGCGGCCCGGGGCCGACATTATCCGGGAAGCCGGGGGG
CTTCATCGTTTCATGCACTGGGAGAGGCCGATTCTTACAGACAGCGGCGGTTTCCAGGTCTTTAGCCTGGCCGACCTGCG
CGAGATCAGCGACGAAGGGGTTACTTTTCGCTCCCACCTGGACGGTTCAATCCATTTCCTGGGGCCGGCGGAGTCCATGG
CTGTCCAGGAAGCCCTGGGCTCGGACATCGCCATGGCCTTCGACGAGTGTGTCGCCTACCCGGCCAGCCCGGAGGAAGTC
GCCGCCGGGGTGGAACGAACCAGCCGCTGGGCCGAGGCCTGCCTCCGGGCCCACCGGCGGGAGGACCAGGCGGTCTTTGG
TATAATTCAGGGAGGGACCATACCCGAACTGCGCCGCCGCAGTGCCCGGGAGATTACCGCCCTGGATTTCCCCGGCTACG
GCATCGGGGGTTTAAGCGTAGGCGAGCCCAAGGAGTTGATGTACAGCATCCTGGAAGAACTCCAGGGCTATCTGCCGGAG
AATAAGCCCCGGTATCTCATGGGGGTCGGTTCCCCTGATTGCCTTATTGAAGGGGTCAAACGAGGGGTGGATATGTTTGA
CTGCGTCTTACCCACCCGCATTGCCCGCAACGGTACGGTGATGACTACCTACGGCAAGCTGGTTGTTCGCAATGCCGCCT
ATGCCCGGGACTTCCGGCCTCTGGACCCGGAGTGCGACTGCTATACCTGCCGTAACTACACCCGCGCCTATATTCGCCAC
CTGCTAAAGGCTGAGGAGATTTTGGGCCTCAGGCTGACGACCATCCATAACCTGCACTTTCTCATTAAACTGATGCAGCG
CCTGCGCCAGGCCATCGCCGAAGGCCGGCTGGAAGAAGTGGCCGCCGACTTTTATGAACGCTATAATTCGGGAAAAATTT
AA

Upstream 100 bases:

>100_bases
CGGGTCGCCGTCCGGGAAGGCTACCGCTTTTACAGTTTCGGCGACGCTATGTTGATCCTGTAAAGAATTTATATGGTAAG
AAAGCTAATTGGAGGAAGAC

Downstream 100 bases:

>100_bases
AAGCAGCAGGTATTCCCGCCGGTACCGTCGAATAGTTAACGGGCTGACCAAAATTTAGAAGGAGTGGTGGTTGGTTTCAT
GCAACAGTGGGCTGGCACTA

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MPAVTFTVLKRDRSTGARLGRLTTPHGTIETPVFMPVGTQATVKTMTPEEVAGLGAEIILANTYHLYLRPGADIIREAGG
LHRFMHWERPILTDSGGFQVFSLADLREISDEGVTFRSHLDGSIHFLGPAESMAVQEALGSDIAMAFDECVAYPASPEEV
AAGVERTSRWAEACLRAHRREDQAVFGIIQGGTIPELRRRSAREITALDFPGYGIGGLSVGEPKELMYSILEELQGYLPE
NKPRYLMGVGSPDCLIEGVKRGVDMFDCVLPTRIARNGTVMTTYGKLVVRNAAYARDFRPLDPECDCYTCRNYTRAYIRH
LLKAEEILGLRLTTIHNLHFLIKLMQRLRQAIAEGRLEEVAADFYERYNSGKI

Sequences:

>Translated_373_residues
MPAVTFTVLKRDRSTGARLGRLTTPHGTIETPVFMPVGTQATVKTMTPEEVAGLGAEIILANTYHLYLRPGADIIREAGG
LHRFMHWERPILTDSGGFQVFSLADLREISDEGVTFRSHLDGSIHFLGPAESMAVQEALGSDIAMAFDECVAYPASPEEV
AAGVERTSRWAEACLRAHRREDQAVFGIIQGGTIPELRRRSAREITALDFPGYGIGGLSVGEPKELMYSILEELQGYLPE
NKPRYLMGVGSPDCLIEGVKRGVDMFDCVLPTRIARNGTVMTTYGKLVVRNAAYARDFRPLDPECDCYTCRNYTRAYIRH
LLKAEEILGLRLTTIHNLHFLIKLMQRLRQAIAEGRLEEVAADFYERYNSGKI
>Mature_372_residues
PAVTFTVLKRDRSTGARLGRLTTPHGTIETPVFMPVGTQATVKTMTPEEVAGLGAEIILANTYHLYLRPGADIIREAGGL
HRFMHWERPILTDSGGFQVFSLADLREISDEGVTFRSHLDGSIHFLGPAESMAVQEALGSDIAMAFDECVAYPASPEEVA
AGVERTSRWAEACLRAHRREDQAVFGIIQGGTIPELRRRSAREITALDFPGYGIGGLSVGEPKELMYSILEELQGYLPEN
KPRYLMGVGSPDCLIEGVKRGVDMFDCVLPTRIARNGTVMTTYGKLVVRNAAYARDFRPLDPECDCYTCRNYTRAYIRHL
LKAEEILGLRLTTIHNLHFLIKLMQRLRQAIAEGRLEEVAADFYERYNSGKI

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=349, Percent_Identity=46.7048710601719, Blast_Score=333, Evalue=2e-91,
Organism=Homo sapiens, GI13375872, Length=278, Percent_Identity=27.3381294964029, Blast_Score=115, Evalue=9e-26,
Organism=Escherichia coli, GI1786607, Length=356, Percent_Identity=55.6179775280899, Blast_Score=404, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17544680, Length=357, Percent_Identity=44.2577030812325, Blast_Score=310, Evalue=9e-85,
Organism=Caenorhabditis elegans, GI25146502, Length=209, Percent_Identity=27.7511961722488, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI19920510, Length=369, Percent_Identity=43.6314363143631, Blast_Score=331, Evalue=6e-91,
Organism=Drosophila melanogaster, GI21355311, Length=224, Percent_Identity=25, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_MOOTA (Q2RHU3)

Other databases:

- EMBL:   CP000232
- RefSeq:   YP_430539.1
- HSSP:   P28720
- ProteinModelPortal:   Q2RHU3
- SMR:   Q2RHU3
- STRING:   Q2RHU3
- GeneID:   3833294
- GenomeReviews:   CP000232_GR
- KEGG:   mta:Moth_1694
- NMPDR:   fig|264732.9.peg.1693
- eggNOG:   COG0343
- HOGENOM:   HBG629929
- OMA:   FMPVGTV
- PhylomeDB:   Q2RHU3
- BioCyc:   MTHE264732:MOTH_1694-MONOMER
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 41525; Mature: 41394

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: ACT_SITE 94-94 BINDING 95-95

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAVTFTVLKRDRSTGARLGRLTTPHGTIETPVFMPVGTQATVKTMTPEEVAGLGAEIIL
CCCEEEEEECCCCCCCHHHCCCCCCCCCCCCCEEEECCCCCEEECCCHHHHHCCCCEEEE
ANTYHLYLRPGADIIREAGGLHRFMHWERPILTDSGGFQVFSLADLREISDEGVTFRSHL
EEEEEEEECCCHHHHHHCCCHHHHHHCCCCEEECCCCEEEEEHHHHHHHHCCCCEEEECC
DGSIHFLGPAESMAVQEALGSDIAMAFDECVAYPASPEEVAAGVERTSRWAEACLRAHRR
CCCEEEECCHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
EDQAVFGIIQGGTIPELRRRSAREITALDFPGYGIGGLSVGEPKELMYSILEELQGYLPE
CCCEEEEEECCCCCHHHHHHHHHCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
NKPRYLMGVGSPDCLIEGVKRGVDMFDCVLPTRIARNGTVMTTYGKLVVRNAAYARDFRP
CCCCEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCC
LDPECDCYTCRNYTRAYIRHLLKAEEILGLRLTTIHNLHFLIKLMQRLRQAIAEGRLEEV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
AADFYERYNSGKI
HHHHHHHHCCCCC
>Mature Secondary Structure 
PAVTFTVLKRDRSTGARLGRLTTPHGTIETPVFMPVGTQATVKTMTPEEVAGLGAEIIL
CCEEEEEECCCCCCCHHHCCCCCCCCCCCCCEEEECCCCCEEECCCHHHHHCCCCEEEE
ANTYHLYLRPGADIIREAGGLHRFMHWERPILTDSGGFQVFSLADLREISDEGVTFRSHL
EEEEEEEECCCHHHHHHCCCHHHHHHCCCCEEECCCCEEEEEHHHHHHHHCCCCEEEECC
DGSIHFLGPAESMAVQEALGSDIAMAFDECVAYPASPEEVAAGVERTSRWAEACLRAHRR
CCCEEEECCHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
EDQAVFGIIQGGTIPELRRRSAREITALDFPGYGIGGLSVGEPKELMYSILEELQGYLPE
CCCEEEEEECCCCCHHHHHHHHHCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
NKPRYLMGVGSPDCLIEGVKRGVDMFDCVLPTRIARNGTVMTTYGKLVVRNAAYARDFRP
CCCCEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCC
LDPECDCYTCRNYTRAYIRHLLKAEEILGLRLTTIHNLHFLIKLMQRLRQAIAEGRLEEV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
AADFYERYNSGKI
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA