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The map label for this gene is glnA [H]

Identifier: 83590142

GI number: 83590142

Start: 1334225

End: 1335559

Strand: Reverse

Name: glnA [H]

Synonym: Moth_1294

Alternate gene names: 83590142

Gene position: 1335559-1334225 (Counterclockwise)

Preceding gene: 83590143

Following gene: 83590141

Centisome position: 50.81

GC content: 58.8

Gene sequence:

>1335_bases
ATGGATGACAAAGAAAAGAGAGCAGCGGTCCTGCAACAGGCCGAGGAATGGGGGGTCAAGTTTGTCCGCCTCCAGTTTAC
GGACATCTTTGGCGTACTCAAAAACGTAGCCATTCCCGTGGACCAGCTCCCCAAGGCCCTCAATAACGAGCTGATGTTCG
ACGGATCTTCAATTGAAGGGTTCGTTCGCATTGAGGAGTCCGATATGTACCTGCGGCCGGACCCGGATACCTTTGTTGTT
TTTCCCTGGCGGCCCCATGAGGGTTCAGTGGCCCGGCTGATCTGTGATGTCTACAACCCCGACGGCACGCCCTTCGCCGG
TTGCCCCCGCTCCACCTTAAAAAGGGTAATGGCCGAAGCGGCGGAAATGGGCTTTACCATGAATGCCGGGCCGGAGGCCG
AGTTTTTCCTCTTCCACACCGACGCCGACGGCCGCCCGACCCTGGAGACCCAGGACCGCGCCGGTTACTTTGACCTGACT
CCGGTGGATCTGGGTGAAGACGCTCGCCGGGATATGGTCCTGACCCTGGAGCAAATGGGCTTTGAGATCGAGGCCTCCCA
CCATGAAGTGGCTCCCGGGCAGCACGAGATTGATTTCAAATATGCCGAGGCCCTGACTACCGCCGACCGGATCGCTACCT
TTAAATTTGTGGTCCGGACCATCGCCCAGCGGCATGGCCTCCACGCCACCTTTATGCCCAAACCCATCTACGGCATCAAC
GGTTCCGGTATGCATGCCAACCTTTCCTTATCTAAAGACGGTAAAAACGCTTTTGACGATCCCTCTGATGAACTGGGCTT
AAGCCAGGTGGCCTACCATTTTATCGCCGGCATCATGGCCCATGCCCGTGCCCTGACGGCCGTTACCAACCCCACGGTTA
ACTCCTACAAGCGCCTGGTGCCGGGTTACGAGGCGCCGGTATACATCGCCTGGTCGCCGCGCAACCGGAGCCCCTTGATC
CGGGTACCGGCCAAGCGGGGGGCCTCCACCCGGATTGAAGTGCGTCACCCGGACCCCTCCTGCAACCCCTACCTGGCCCT
GGCTGTTCTCTTGAAGGCCGGTCTCGATGGTATCAAAAAGGGCCTGACACCACCGCCGCCGACGGATAAAAACATCTTTG
CTATGACCCCGGCGGAGCTTAAGGCAGAAGGAATCGGCGTCCTGCCCGGCAGCCTGGAGGAGGCCCTGGCGGCCTTGGAG
CAAGATGAAGTCATCCGCGAGGCCCTGGGACCCCATATCTACGAACGCTTGACCCTGGCTCAAAAGATGGAATGCGAGGA
GTATCGCACCCGGGTCCACCAGTGGGAGATTGACCAGTACTTGACTAAATTTTAA

Upstream 100 bases:

>100_bases
CAGGCCGGAGAAAGCAGGCTTGGAAAACCAGCAAGTATCTGGCGGTAGTAGGGTAGCTCTCCACCAATAAGGAAGCACAT
TCTTGAAGGAGGTAAATTCC

Downstream 100 bases:

>100_bases
CCGGGGGCGCCTATGCCGCCCGGACGGCACCATATTAGATGCCAGTCGAGGAATAGAATAATTATACGCGGCTGTTATCA
TGGTGGGCAGCGACATTTTG

Product: L-glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MDDKEKRAAVLQQAEEWGVKFVRLQFTDIFGVLKNVAIPVDQLPKALNNELMFDGSSIEGFVRIEESDMYLRPDPDTFVV
FPWRPHEGSVARLICDVYNPDGTPFAGCPRSTLKRVMAEAAEMGFTMNAGPEAEFFLFHTDADGRPTLETQDRAGYFDLT
PVDLGEDARRDMVLTLEQMGFEIEASHHEVAPGQHEIDFKYAEALTTADRIATFKFVVRTIAQRHGLHATFMPKPIYGIN
GSGMHANLSLSKDGKNAFDDPSDELGLSQVAYHFIAGIMAHARALTAVTNPTVNSYKRLVPGYEAPVYIAWSPRNRSPLI
RVPAKRGASTRIEVRHPDPSCNPYLALAVLLKAGLDGIKKGLTPPPPTDKNIFAMTPAELKAEGIGVLPGSLEEALAALE
QDEVIREALGPHIYERLTLAQKMECEEYRTRVHQWEIDQYLTKF

Sequences:

>Translated_444_residues
MDDKEKRAAVLQQAEEWGVKFVRLQFTDIFGVLKNVAIPVDQLPKALNNELMFDGSSIEGFVRIEESDMYLRPDPDTFVV
FPWRPHEGSVARLICDVYNPDGTPFAGCPRSTLKRVMAEAAEMGFTMNAGPEAEFFLFHTDADGRPTLETQDRAGYFDLT
PVDLGEDARRDMVLTLEQMGFEIEASHHEVAPGQHEIDFKYAEALTTADRIATFKFVVRTIAQRHGLHATFMPKPIYGIN
GSGMHANLSLSKDGKNAFDDPSDELGLSQVAYHFIAGIMAHARALTAVTNPTVNSYKRLVPGYEAPVYIAWSPRNRSPLI
RVPAKRGASTRIEVRHPDPSCNPYLALAVLLKAGLDGIKKGLTPPPPTDKNIFAMTPAELKAEGIGVLPGSLEEALAALE
QDEVIREALGPHIYERLTLAQKMECEEYRTRVHQWEIDQYLTKF
>Mature_444_residues
MDDKEKRAAVLQQAEEWGVKFVRLQFTDIFGVLKNVAIPVDQLPKALNNELMFDGSSIEGFVRIEESDMYLRPDPDTFVV
FPWRPHEGSVARLICDVYNPDGTPFAGCPRSTLKRVMAEAAEMGFTMNAGPEAEFFLFHTDADGRPTLETQDRAGYFDLT
PVDLGEDARRDMVLTLEQMGFEIEASHHEVAPGQHEIDFKYAEALTTADRIATFKFVVRTIAQRHGLHATFMPKPIYGIN
GSGMHANLSLSKDGKNAFDDPSDELGLSQVAYHFIAGIMAHARALTAVTNPTVNSYKRLVPGYEAPVYIAWSPRNRSPLI
RVPAKRGASTRIEVRHPDPSCNPYLALAVLLKAGLDGIKKGLTPPPPTDKNIFAMTPAELKAEGIGVLPGSLEEALAALE
QDEVIREALGPHIYERLTLAQKMECEEYRTRVHQWEIDQYLTKF

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=432, Percent_Identity=25.6944444444444, Blast_Score=135, Evalue=7e-32,
Organism=Escherichia coli, GI1790301, Length=435, Percent_Identity=44.367816091954, Blast_Score=303, Evalue=2e-83,
Organism=Escherichia coli, GI87081870, Length=425, Percent_Identity=27.0588235294118, Blast_Score=123, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6325292, Length=334, Percent_Identity=26.3473053892216, Blast_Score=71, Evalue=3e-13,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 49470; Mature: 49470

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDKEKRAAVLQQAEEWGVKFVRLQFTDIFGVLKNVAIPVDQLPKALNNELMFDGSSIEG
CCCHHHHHHHHHHHHHCCEEEEEEEHHHHHHHHHHCCCCHHHHHHHHCCCEEECCCCCCE
FVRIEESDMYLRPDPDTFVVFPWRPHEGSVARLICDVYNPDGTPFAGCPRSTLKRVMAEA
EEEEECCCEEECCCCCEEEEEECCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHH
AEMGFTMNAGPEAEFFLFHTDADGRPTLETQDRAGYFDLTPVDLGEDARRDMVLTLEQMG
HHCCEEECCCCCCEEEEEEECCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHEEEHHHCC
FEIEASHHEVAPGQHEIDFKYAEALTTADRIATFKFVVRTIAQRHGLHATFMPKPIYGIN
CEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCC
GSGMHANLSLSKDGKNAFDDPSDELGLSQVAYHFIAGIMAHARALTAVTNPTVNSYKRLV
CCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHC
PGYEAPVYIAWSPRNRSPLIRVPAKRGASTRIEVRHPDPSCNPYLALAVLLKAGLDGIKK
CCCCCCEEEEECCCCCCCEEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCHHHHHH
GLTPPPPTDKNIFAMTPAELKAEGIGVLPGSLEEALAALEQDEVIREALGPHIYERLTLA
CCCCCCCCCCCEEEECCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
QKMECEEYRTRVHQWEIDQYLTKF
HHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDDKEKRAAVLQQAEEWGVKFVRLQFTDIFGVLKNVAIPVDQLPKALNNELMFDGSSIEG
CCCHHHHHHHHHHHHHCCEEEEEEEHHHHHHHHHHCCCCHHHHHHHHCCCEEECCCCCCE
FVRIEESDMYLRPDPDTFVVFPWRPHEGSVARLICDVYNPDGTPFAGCPRSTLKRVMAEA
EEEEECCCEEECCCCCEEEEEECCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHH
AEMGFTMNAGPEAEFFLFHTDADGRPTLETQDRAGYFDLTPVDLGEDARRDMVLTLEQMG
HHCCEEECCCCCCEEEEEEECCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHEEEHHHCC
FEIEASHHEVAPGQHEIDFKYAEALTTADRIATFKFVVRTIAQRHGLHATFMPKPIYGIN
CEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCC
GSGMHANLSLSKDGKNAFDDPSDELGLSQVAYHFIAGIMAHARALTAVTNPTVNSYKRLV
CCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHC
PGYEAPVYIAWSPRNRSPLIRVPAKRGASTRIEVRHPDPSCNPYLALAVLLKAGLDGIKK
CCCCCCEEEEECCCCCCCEEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCHHHHHH
GLTPPPPTDKNIFAMTPAELKAEGIGVLPGSLEEALAALEQDEVIREALGPHIYERLTLA
CCCCCCCCCCCEEEECCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
QKMECEEYRTRVHQWEIDQYLTKF
HHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2891680; 1981087 [H]