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The map label for this gene is aspA

Identifier: 83590121

GI number: 83590121

Start: 1316165

End: 1317595

Strand: Reverse

Name: aspA

Synonym: Moth_1273

Alternate gene names: 83590121

Gene position: 1317595-1316165 (Counterclockwise)

Preceding gene: 83590122

Following gene: 83590120

Centisome position: 50.12

GC content: 60.1

Gene sequence:

>1431_bases
ATGTCTACCCGCCAGGAACACGACCTGCTGGGCACCAGGGAAGTACCAGCTACTGCTTATTATGGTATCCATACCCTGCG
GGCCGCAGAAAACTTCAACGTCAGCCGGGCCAGGGTCCATCCGGAATTGATTAAAGCCCTGGCTACTGTAAAAGAAGCCG
CGGCCAGGGCTAACCTGGACCTGGGTTACCTGCCGGCCGAAAAAGGCCGGGCCATCATCACCGCCTGCCAGGAAGTGGCC
CGGGGTGAACTGGCCGACCAGTTTTTCCTCGACGCCTACCAGGGCGGCGCCGGTACCTCAACCAATATGAACGTCAACGA
GGTAATCGCCAACCGCGCCCTGGAAATTCTGGGTCGCCCCAAAGGCGATTACGCTACCATCCATCCCATCGATCACGTTA
ACCTGCATCAGTCCACTAACGATGTCTACCCCACGGCCATGCGGGTGGCGGCCATCCGCCTGTTGCTGCCCCTGGCGGAT
GAACTGGCGAAACTCCAGGAAGCCCTCCAGGAGAAAGAGGCCGCCTTCGCCGGGGTGGTCAAAATCGGTCGTACCGAGCT
CCAGGACGCCGTGCCGGTAACCCTGGGGCAGGAATTCGGCGCTTACGCCCAGGCCATTTCCCGGGACCGCTGGCGCCTCT
ATAAAGTTGAAGAGCGCCTGCGCCAGGTAAACCTGGGTGGCACCGCCACCGGCACCGGCCTTAACGCCCCCCTGAAGTAC
ATCTACCTGGTCAACGACTACCTGCGCCGCCTTACAGGAATAGGCCTGGCCCGGGCGGAGAATATGATTGACGCCACCCA
GAATATGGACGTCTTTGTGGAGGTCTCTGGTCTGGTCAAGGCTGCCGCCGTTACCATGCACAAAATAGCCTCCGACCTGC
GTTTTATGGCCGCCGGCCCCCGGGGCGGCCCGGCGGAGATCAATTTGCCGGAACGCCAGGCGGGATCCTCCATCATGCCC
GGCAAAGTCAATCCCGTCATCCCGGAGATGGTCAGCCAGGTAGCCATGCAGGTCATGGCCAATGATTACTTGATCGCCAT
GGCTGCCAGTCAGGGCCAGCTGGAGCTCAATCCCTTTGCCCCCCTTATTGCCCATACCTTGCTGGAATCCCTGGCCATGC
TGGCGGCAGCGGCCCGGATATTCCGCACCGAGTGTATCACGGGTATAACCGCCAACCCCGAGCGCTGCCAGGAACTCCTG
GCCGTGAGCCCGGCCCTGGCTACGGCCCTGCTGCCCTATATTGGCTACGAGAAGGCCACGGAAGTAGTGCGGGAAGCCGT
GGTTTCCGGCCGATCAATAAAAGAAATAGTTCTAGAAGAAGGGTATTTGACCTCTGACGAACTGGAAAACGTCTTAACCC
CGGCCGCCATGACCAAACCGGGAACCGTCGGAGCCGTAAAGCAGGGAATAAAGGAGAAGGGAAAAGCGTAA

Upstream 100 bases:

>100_bases
TGGTAGGCATATTATCGCTAAATTATCCCGGAAGCGATTGGCATTTAATTGGCCCGTCGCTTTTTGTTTGCTAAAATAGG
GGTAGAGGAAGGGATTGTCC

Downstream 100 bases:

>100_bases
CTGGTTGAGGACGAACCTTGCCCCCACCGTTTCCAGCGCCTATGATAGACTGCAAGCATATCATAGGTTGGGGGTGTTCG
AAATGACCAGAATTGGTATT

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MSTRQEHDLLGTREVPATAYYGIHTLRAAENFNVSRARVHPELIKALATVKEAAARANLDLGYLPAEKGRAIITACQEVA
RGELADQFFLDAYQGGAGTSTNMNVNEVIANRALEILGRPKGDYATIHPIDHVNLHQSTNDVYPTAMRVAAIRLLLPLAD
ELAKLQEALQEKEAAFAGVVKIGRTELQDAVPVTLGQEFGAYAQAISRDRWRLYKVEERLRQVNLGGTATGTGLNAPLKY
IYLVNDYLRRLTGIGLARAENMIDATQNMDVFVEVSGLVKAAAVTMHKIASDLRFMAAGPRGGPAEINLPERQAGSSIMP
GKVNPVIPEMVSQVAMQVMANDYLIAMAASQGQLELNPFAPLIAHTLLESLAMLAAAARIFRTECITGITANPERCQELL
AVSPALATALLPYIGYEKATEVVREAVVSGRSIKEIVLEEGYLTSDELENVLTPAAMTKPGTVGAVKQGIKEKGKA

Sequences:

>Translated_476_residues
MSTRQEHDLLGTREVPATAYYGIHTLRAAENFNVSRARVHPELIKALATVKEAAARANLDLGYLPAEKGRAIITACQEVA
RGELADQFFLDAYQGGAGTSTNMNVNEVIANRALEILGRPKGDYATIHPIDHVNLHQSTNDVYPTAMRVAAIRLLLPLAD
ELAKLQEALQEKEAAFAGVVKIGRTELQDAVPVTLGQEFGAYAQAISRDRWRLYKVEERLRQVNLGGTATGTGLNAPLKY
IYLVNDYLRRLTGIGLARAENMIDATQNMDVFVEVSGLVKAAAVTMHKIASDLRFMAAGPRGGPAEINLPERQAGSSIMP
GKVNPVIPEMVSQVAMQVMANDYLIAMAASQGQLELNPFAPLIAHTLLESLAMLAAAARIFRTECITGITANPERCQELL
AVSPALATALLPYIGYEKATEVVREAVVSGRSIKEIVLEEGYLTSDELENVLTPAAMTKPGTVGAVKQGIKEKGKA
>Mature_475_residues
STRQEHDLLGTREVPATAYYGIHTLRAAENFNVSRARVHPELIKALATVKEAAARANLDLGYLPAEKGRAIITACQEVAR
GELADQFFLDAYQGGAGTSTNMNVNEVIANRALEILGRPKGDYATIHPIDHVNLHQSTNDVYPTAMRVAAIRLLLPLADE
LAKLQEALQEKEAAFAGVVKIGRTELQDAVPVTLGQEFGAYAQAISRDRWRLYKVEERLRQVNLGGTATGTGLNAPLKYI
YLVNDYLRRLTGIGLARAENMIDATQNMDVFVEVSGLVKAAAVTMHKIASDLRFMAAGPRGGPAEINLPERQAGSSIMPG
KVNPVIPEMVSQVAMQVMANDYLIAMAASQGQLELNPFAPLIAHTLLESLAMLAAAARIFRTECITGITANPERCQELLA
VSPALATALLPYIGYEKATEVVREAVVSGRSIKEIVLEEGYLTSDELENVLTPAAMTKPGTVGAVKQGIKEKGKA

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=465, Percent_Identity=43.2258064516129, Blast_Score=364, Evalue=1e-100,
Organism=Escherichia coli, GI87082375, Length=462, Percent_Identity=47.1861471861472, Blast_Score=407, Evalue=1e-114,
Organism=Escherichia coli, GI1787896, Length=472, Percent_Identity=42.3728813559322, Blast_Score=351, Evalue=6e-98,
Organism=Caenorhabditis elegans, GI17553882, Length=463, Percent_Identity=41.9006479481641, Blast_Score=343, Evalue=9e-95,
Organism=Caenorhabditis elegans, GI32565146, Length=330, Percent_Identity=40.6060606060606, Blast_Score=242, Evalue=3e-64,
Organism=Saccharomyces cerevisiae, GI6324993, Length=462, Percent_Identity=43.0735930735931, Blast_Score=356, Evalue=5e-99,
Organism=Drosophila melanogaster, GI24640179, Length=461, Percent_Identity=40.5639913232104, Blast_Score=342, Evalue=2e-94,
Organism=Drosophila melanogaster, GI24640177, Length=461, Percent_Identity=40.5639913232104, Blast_Score=342, Evalue=3e-94,
Organism=Drosophila melanogaster, GI24662684, Length=465, Percent_Identity=39.5698924731183, Blast_Score=329, Evalue=2e-90,
Organism=Drosophila melanogaster, GI78710009, Length=462, Percent_Identity=38.7445887445887, Blast_Score=321, Evalue=6e-88,
Organism=Drosophila melanogaster, GI24583245, Length=463, Percent_Identity=36.9330453563715, Blast_Score=300, Evalue=2e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 51350; Mature: 51219

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTRQEHDLLGTREVPATAYYGIHTLRAAENFNVSRARVHPELIKALATVKEAAARANLD
CCCCCHHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCC
LGYLPAEKGRAIITACQEVARGELADQFFLDAYQGGAGTSTNMNVNEVIANRALEILGRP
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCC
KGDYATIHPIDHVNLHQSTNDVYPTAMRVAAIRLLLPLADELAKLQEALQEKEAAFAGVV
CCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIGRTELQDAVPVTLGQEFGAYAQAISRDRWRLYKVEERLRQVNLGGTATGTGLNAPLKY
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHH
IYLVNDYLRRLTGIGLARAENMIDATQNMDVFVEVSGLVKAAAVTMHKIASDLRFMAAGP
HHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCC
RGGPAEINLPERQAGSSIMPGKVNPVIPEMVSQVAMQVMANDYLIAMAASQGQLELNPFA
CCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCEEECCHH
PLIAHTLLESLAMLAAAARIFRTECITGITANPERCQELLAVSPALATALLPYIGYEKAT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHH
EVVREAVVSGRSIKEIVLEEGYLTSDELENVLTPAAMTKPGTVGAVKQGIKEKGKA
HHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHCCHHCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
STRQEHDLLGTREVPATAYYGIHTLRAAENFNVSRARVHPELIKALATVKEAAARANLD
CCCCHHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCC
LGYLPAEKGRAIITACQEVARGELADQFFLDAYQGGAGTSTNMNVNEVIANRALEILGRP
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCC
KGDYATIHPIDHVNLHQSTNDVYPTAMRVAAIRLLLPLADELAKLQEALQEKEAAFAGVV
CCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIGRTELQDAVPVTLGQEFGAYAQAISRDRWRLYKVEERLRQVNLGGTATGTGLNAPLKY
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHH
IYLVNDYLRRLTGIGLARAENMIDATQNMDVFVEVSGLVKAAAVTMHKIASDLRFMAAGP
HHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCC
RGGPAEINLPERQAGSSIMPGKVNPVIPEMVSQVAMQVMANDYLIAMAASQGQLELNPFA
CCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCEEECCHH
PLIAHTLLESLAMLAAAARIFRTECITGITANPERCQELLAVSPALATALLPYIGYEKAT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHH
EVVREAVVSGRSIKEIVLEEGYLTSDELENVLTPAAMTKPGTVGAVKQGIKEKGKA
HHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHCCHHCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3096982 [H]